靶向Neuropilin-1的抗肿瘤研究进展

Neuropilin-1(NRP-1)又称神经鞭毛素蛋白,是一个大小为130～140 kD的非酪氨酸激酶跨膜蛋白,参与脊椎动物胚胎的神经和心血管系统发育。它作为一个多功能受体调节VEGF(血管内皮生长因子),PDGF(血小板衍生生长因子),bFGF(成纤维生长因子)等细胞因子的信号通路,与肿瘤血管新生和肿瘤转移密切相关。NRP-1被认为是一个新的肿瘤治疗靶点,近年来针对NRP-1为靶点的药物研究有不少新进展。本文主要介绍NRP-1的生物学特征,对肿瘤作用以及相关药物研究

靶向Neuropilin-1的抗肿瘤研究进展

Neuropilin-1(NRP-1)又称神经鞭毛素蛋白,是一个大小为130～140 kD的非酪氨酸激酶跨膜蛋白,参与脊椎动物胚胎的神经和心血管系统发育。它作为一个多功能受体调节VEGF(血管内皮生长因子),PDGF(血小板衍生生长因子),bFGF(成纤维生长因子)等细胞因子的信号通路,与肿瘤血管新生和肿瘤转移密切相关。NRP-1被认为是一个新的肿瘤治疗靶点,近年来针对NRP-1为靶点的药物研究有不少新进展。本文主要介绍NRP-1的生物学特征,对肿瘤作用以及相关药物研究

抗人NRP1单克隆抗体的活性鉴定及初步应用

目的鉴定抗人神经菌毛素1(neuropilin1,NRP1)单克隆抗体的生物活性并利用该抗体检测常见肿瘤细胞株NRP1蛋白的表达情况。方法腹水法制备4株抗NRP1单克隆抗体并用rProtein A亲和柱纯化抗体,间接ELISA检测4株抗体的滴度水平,Western blot分析4株抗体结合NRP1蛋白的特异性,流式细胞术分析4株抗体结合NRP1蛋白的亲和力,共聚焦免疫荧光实验进一步验证抗体结合NRP1蛋白的能力;细胞免疫组织化学染色检测14株肿瘤细胞表达NRP1蛋白的情况。结果 4株抗体均能特异结合NRP1蛋白,其中A6结合NRP1能力最强,能结合在表达NRP1蛋白的细胞膜表面。细胞免疫组织化学染色结果显示NRP1在HepG2、C6、HEK293、BEL-7402、MDA-MB-453呈高水平表达,在U87、MGC803、MCF7、MDA-MB-231中表达水平相对较弱,而在U251、BGC823、H6、HT-29的表达介于两者之间,在SMMC-7721细胞中未检测到表达。结论 4株抗NRP1单克隆抗体均能特异结合NRP1蛋白,多种肿瘤细胞株均不同程度表达NRP1蛋白,为进一步探讨NRP1蛋白与肿瘤发生发展的关系奠定了基础

策勒绿洲四种杨树的生态生理学特性的研究

本文通过四种杨种（新疆杨Populus alba L.var. Pyramidalis Bge.、箭杆杨Populus nigra L var. thevestina (Dode) Bean、胡杨Populus euphratica Oliv和灰杨Populus pruinosa. Schrank）的清晨带叶小枝水势月变化、叶片中叶绿素含量的月变化、光合作用和蒸腾作用的日变化、P-V曲线及持水力的测定分析，得出了如下初步结论：1 在测定的四种杨柳树中，新疆杨清晨水势变幅最大。这说明新疆杨对土壤对水分含量的变化最为敏感。新疆杨短枝叶的水势低于长枝叶，主要是由于短枝叶叶龄远大于长枝叶所致。其余三种杨树水势的月际变幅较大，说明了它们调节体内水份状况的能力较强。2 新疆杨和箭杆杨是人式引进的栽培种，而胡杨和灰杨为当地的乡土种。根据P-V曲线有关参数及持水力的测定值，人工栽培种新疆杨和箭植树杨的抗旱性能低于终年土种胡杨和灰杨，这是胡杨和灰杨长期适应当地生态环境条件的结果3 四种杨种的水分利用效率的排序是：胡杨>箭杆杨>灰杨>新疆杨。从这四种杨树的综合生态生理指标来看，胡杨和灰杨具有较高的水分利用效率和相对较好的适应干旱的机制，具备生长和生存上的优势。但因育苗困难，上前尚难于作为主要造林树种；箭植树杨对水分胁迫较为敏感，而新疆具有较强的体内水势调节能力，在塔南春季非常缺水的条件下，应以新疆杨作为首选造林树种。而在能充分满足灌溉的条件下，应首选箭杆杨，因为箭杆杆杨的水分利用效率高于新疆杨。Ecophysiological characteristics of Populus alba L. var. Pyramidalis Bge., Populus nigra L var. thevestina (Dode) Bean、Populus euphratica Oliv and Populus pruinosa Schrank has been studied in this paper. The monthly changes of predawn water potential of xylem and content of Chlorophyll, diurnal course of photosynthesis rate and transpiration rate, P-V curves and water-holding ability of four poplars were measured. Our results suggest: 1 The variant range vaule of predawn water potential of xylem of P. alba L. var. Pyramidalis was the greatest among four poplars., which showed that P. alba L. var. Pyramidalis is the most sensitive to the change of content of soil water among four poplars. The predawn water potential of short-shoot leaves of P. alba L. var. Pyramidalis is lower than that long-shoot leaves of P. alba L. var. Pyramidalis. This resulted from the leaf age of short-shoot leaves of P. alba L. var. Pyramidalis is older than leaf age of long-shoot leaves of P. alba L. var. Pyramidalis And the variant range values between mouthes of predawn water potential of xylem of P. nigra L var. thevestina, P. euphratica and P. pruinosa were smaller, which showed that they had relative strong ability to regulate water condition in their bodies. 2 P. alba L. var. Pyramidalis and P. nigra L var. thevestina were introduced poplar species. While P. euphratica and P. pruinosa are native species. According to the parameters of P-V curves and the measured vaules of water-holding ability, the ability of drought-resistance of P. alba L. var. Pyramidalis and P. nigra L var. thevestina(introduced species) is weaker than that of P. euphratica and p. pruinosa(native species). This was the result that P. euphratica and P. Pruinosa adjust the environment for a long time. 3 The order of water use efficiency (WUE) of four poplar is: P. euphratica> P. nigra L var. thevestina> P. pruinosa> P. alba L. var. Pyramidalis. From all the standards of ecophyology of four poplars, P. euphratica and P. pruinosa have the higher WUE and the better regime of drought-resistance. But it is difficulty to cultivate the seedlings of P. euphratica and P. pruinosa. For P. alba L. var. Pyramidalis has relative stronger ability to adjust water potential in its body, we should choose it as the important species to form the system of shelter-forest in the shortage of water in Spring in the North of Takalamakan Desert.. And we Should choose P. nigra L var. thevestina as the important species to form the system of shelter-forest under the circumstance of good water supply, because the WUE of P. nigra L var. thevestina is higher than that of P. alba L. var. Pyramidalis

骆驼刺根系生态学

生长指标测定、样品采集分析的基础上，对骆驼刺从种子萌发到开花结果的整个生活史中形态可塑性、盐分的适应特征、化学计量学、根系的拓扑结构、垂直根系生长的水分阈值、生物量的分配策略、伊苏模型、植被修复的最佳灌溉量、生物固氮和氮素分配、水分来源、分株规律等方面进行了系统的定位研究。研究成果将为干旱区深根植物对水分的适应途径和响应策略提供理论依据，同时将为荒漠生态系统的植被修复提供技术方法。 本书可供根系生态学、植物生态学、植物生理生态学等领域的科研人员和其他相关专业本科生、研究生以及关注深根植物、植被修复的各级生产和管理人员参考。<br /