In situ FTIR Spectroscopic Studies of (Bi)sulfate Adsorption and Electrocatalytic Reaction of Aspartic Acid on Nanostructured Metal Electrodes

本论文运用电化学循环伏安法(CV)和原位红外反射光谱(insituFTIRS)系统地研究了硫酸阴离子在各种反射率基底上的Pt纳米粒子电极(nm-Pt/S(n)，S=GC，Pt，Au)表面的吸附，以及不同pH值溶液中门冬氨酸(Asp)在本体Au和纳米Au电极表面的电催化过程，主要结果如下： 1、硫酸阴离子在纳米Pt电极上的吸附。首次发现所制备的纳米Pt电极对硫酸阴离子的吸附表现出异常红外效应(AIREs)，即吸附态的硫酸阴离子在nm-Pt/S(n)电极上给出谱峰方向倒反、谱峰强度增强的红外光谱特征。该研究不仅首次将AIREs现象由强吸附物种(如CO、CN-和SCN-等)拓展到弱吸附物种(硫酸阴...Cyclic voltammetry (CV) and in situ FTIR spectroscopy (in situ FTIRS) were applied to the study of the adsorption of (bi)sulfate on Pt nanoparticles substrated on materials with different IR reflectivity (nm-Pt/S(n), S = GC, Pt, Au) and electrocatalytic reaction of aspartic acid (Asp) on bulk and nanostructured Au electrodes. The main results are summarized below. 1. Study of the adsorption of (b...学位：理学博士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：B20032502

Analysis and Design of Personnel Management Information System for a College

经济和技术的快速发展，带动着社会各方面的快速发展，高等教育制度面临着新一轮的改革，这样一来，高校人事管理制度的完善和创新对于高等教育的发展有着重要作用。现代人事管理系统都是以三大技术（网络技术、信息技术以及计算机技术）为基础的前提下建立起来的，人事管理系统的建设为高校人事管理带来了前所未有的改观和贡献，促进了高校人事管理的科学化、现代化和统一化，大大提高了高校人事管理效率。 本论文利用现有较成熟的计算机技术并结合高校人事管理的实际需求以及国内外发展现状，完成了高等院校人事管理系统的分析和设计工作。系统较充分地考虑了高校人事管理的日常管理需求、管理人员的方便性、互操作性。不仅能够为用户提供很好...The increasingly development of economic and technology, driving the rapid development of various aspects in the society, system of higher education is facing a new round of reform, so the improvement and innovation of higher institution personnel management system plays an important role. Modern personnel management system is based on three major technology, network technology, information techno...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201223006

从政治参与的角度考察我国坚持陪审制度的现实性

陪审制度的本质是一种重要的民主政治的政治思想和司法功能的体现,陪审制度的确立和完善,是人类走向民主文明的司法形式之一。本文认为重新认识陪审制度的价值双重性,彰显陪审制度的政治价值与司法价值,在推进公众政治参与需求下,完善我国的人民陪审制,已然成为法学理论界及司法实务界应重新认识的重要课题

Ⅰ级粉煤灰对水泥净浆开裂的影响

采用环约束法,试验研究了Ⅰ级粉煤灰对不同水胶比下水泥净浆开裂的影响。试验研究发现:Ⅰ级粉煤灰在低水胶比(0.24)情况下对净浆开裂几乎没有影响,当水胶比为0.32和0.40时抗裂作用明显;较高水胶比(0.32、0.40)情况下,Ⅰ级粉煤灰对净浆的抗裂性能有较好的改善作用,在20%～65%掺量范围内,随着掺量的增大,净浆的开裂龄期延长,抗裂性能提高

Assessment of impact of intensive livestock cultivation on water environment in mountain and hilly areas of South China

[摘要]选择南方山地丘陵区典型规模畜禽养殖集中地为研究区域, 通过对该研究区域周边地表水及地下水的 调查采样, 分析主要污染物时空变异及污染物形态之间的变化, 并对周边水环境进行初步评价。结果表明: 规模化畜禽养殖区域的浅层地下水和地表水已经受到严重污染; 养殖场的环保处理设施与养殖规模不配套 造成大量污染物进入地表水环境; 生物氧化塘的护堤和塘底未经防渗处理, 造成下游地下水污染; 养殖场排 放的污水经厌氧和好氧生物处理后富含有机质、氨氮、可溶态有机磷, 利于作物吸收和生长, 应尽量循环使 用。指出由于养殖污水变化较大, 为避免二次环境污染, 必须处理达标后结合配方施肥技术按需回用农田。[Abstract]By selecting a typical site of intensive livestock cultivation in a mountain area of South China as the study area, and investigating and sampling surface and groundwater in the study area, the spatial and temporal variat ions of the main pollutants and changes among pollutant forms were analyzed. The environmental effects of intensive livestock cultivation on surrounding water environments were evaluated. The results indicated that the shallow groundwater and surfacewater were heavily contaminated in the intensive livestock cultivation area. Large amounts of the pollutants entered the surface water, due to the fact that the wastewater treatment facility did not match the scale of livestock cult ivation. The lack of liner treatment of the bank and liner of the lagoon resulted in groundwater pollution. The effluent wastewater from the culturing farm after anaerobic and aerobic biologic treatment was full of organic matter, ammonia nitrogen, and dissolved organic phosphorus, which aided the growth of plants and should be recycled. Because of the significant change of the wastewater component, and in order to avoid secondary pollution, the wastewater should be recycled to land combined with precision fertilizer formula technology after treatment to bring it to the standard.福建省重大科技项目(2002H009)；福州大学人才基金项目(XRC-0611

Electrochemical behavior of p-dihydroxybenzene in DMPC biomimetic membrane

在金电极上构筑一种双肉豆蔻磷脂酰胆碱(DMPC)仿生浇铸膜。经椭圆偏振仪和电化学方法测量证实, DMPC仿生膜是较为致密的多层膜,同时对苯二酚能够透过DMPC仿生膜到达金电极表面进行氧化反应。结果表明,对苯二酚不仅可在脂质膜中进行电子转移,而且是一种重要的生物分子。反应在仿生环境内进行,也为研究生物小分子在真正的生物体内的反应提供有益的帮助。本文探讨对苯二酚在仿生膜修饰电极上的电化学行为,为生物膜中的电子转移过程提供了十分重要的信息。The casting method to form dimyristoyl phosphatidyl choline(DMPC)mimetic biomembrane on an Au electrode is proposed.The results of ellipsometer and electrochemical methods confirmed that the film is a compact membrane that can block the electron transferring process,p-Dihydroxybenzene can reach the electrode surface and occur redox reactions by passing through transient defects in individual bilayers.Such defects occur in biomembranes and are accentuated by relatively small electric fields.The electrochemical behavior of p-dihydroxybenzene in this membrane was also investigated,p-Dihydroxybenzene is biologically important molecule because of its function of transferring electrons in lipid layers.This reaction occur in the bionic environment,which provide the useful help to study the real reaction of small molecules in the biological organisms.Studies on the electrochemical behavior of p-dihydroxybenzene in the DMPC biomimetic membrane provide useful information for elucidating biological electron transfer processes concerning lipid layers.国家自然科学基金项目(20833005,20873116)。~

纤维蛋白原在Pt电极上的界面电化学研究

应用电化学方法和电化学原位红外反射光谱(electrochemical in-situ FTIR)等研究了纤维蛋白原在Pt电极上的界面电化学行为.结果表明:纤维蛋白原在Pt电极上的吸附使电极的析氢与氧脱附过程减弱,影响程度随扫速的增加而增强;同样纤维蛋白原的吸附会降低亚铁氰化钾-铁氰化钾电对的氧化还原反应可逆性和电流;在-0.1~0.6V(vs.SCE)扫描范围内没有出现纤维蛋白原的特征"氧化还原"峰.电化学原位红外反射光谱测试表明纤维蛋白原在0.3~0.5V(vs.SCE)间发生化学反应,有新的产物生成

马铃薯遗传转化系统的优化及禽流感病毒(H5N1)基因的导入

通过对以马铃薯 (Solanum tuberosum L .)块茎为受体的农杆菌转化系统进行优化 ,筛选出使三个品种 (鲁引 1号、克新 3号、优金 )的受体均可得到高频分化的培养基 :MS+2 .0 mg/L BA+3.0mg/L ZT+0 .5mg/L NAA+0 .5mg/L GA.对几种农杆菌转化条件的比较研究发现 ,菌体用 Ms+0 .5mg/L GA液体重悬并稀释 4倍 ,与受体于 2 8℃ ,10 0 r/min振荡侵染 2 0～ 30 min,共培养时在分化培养基上加 30 μ mol/L AS(乙酰丁香酮 ) ,16 h弱光照共培养 3d,可使马铃薯品种鲁引 1号的转化效率较常规方法提高 5～ 7倍 .将禽流感病毒 (H5N1)基因与 35S启动子及 nos终止子构建表达载体 p130 1H5N1,直接法转入农杆菌 EHA10 5,用此菌株转化马铃薯得到再生植株 .基因组总 DNA经 PCR和 Southern杂交证明目的基因已整合到马铃薯基因组

ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage

RhoB作为抑癌蛋白通过诱导肿瘤细胞的凋亡在抑制肿瘤的发生发展中发挥着重要作用，并且与肿瘤耐药性密切相关，但关于RhoB如何促进细胞死亡的分子机理的研究仍不清楚。在本研究中，该团队发现在DNA单链损伤情况下，ATR-Chk1信号通路的激活，会使RhoB被Chk1磷酸化，该磷酸化修饰会使RhoB从细胞质膜解离下来进入细胞质中，进而被SUMO化修饰。SUMO化修饰后的RhoB会与TSC2形成复合物，并将TSC2复合物带到溶酶体上，引起细胞自噬的发生。 该文共同第一作者为刘明冬、曾涛玲和张新，通讯作者为王洪睿教授和赵同金教授。【Abstract】DNA damage can induce autophagy; however, the underlying mechanism remains largely unknown. Here we report that DNA damage leads to autophagy through ATR/Chk1/RhoB-mediated lysosomal recruitment of TSC complex and subsequent mTORC1 inhibition. DNA damage caused by ultraviolet light (UV) or alkylating agent methyl methanesulphonate (MMS) results in phosphorylation of small GTPase RhoB by Chk1. Phosphorylation of RhoB enhances its interaction with the TSC2, and promotes its sumoylation by PIAS1, which is required for RhoB/TSC complex to translocate to lysosomes. As a result, mTORC1 is inhibited, and autophagy is activated. Knockout of RhoB severely attenuates lysosomal translocation of TSC complex and the DNA damage-induced autophagy. Reintroducing wild-type but not sumoylation-resistant RhoB into RhoB−/− cells restores the onset of autophagy. Hence, our study identifies a molecular mechanism for translocation of TSC complex to lysosomes in response to DNA damage, which depends on ATR/Chk1-mediated RhoB phosphorylation and sumoylation.This work was supported by the National Natural Science Foundation of China (U1605222, 81472459, 31671223), the National Key Research and Development Project of China (2016YFC1302400, 2016YFA0502003), the Fundamental Research Funds for the Central Universities (20720140550, 20720160070), the National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China (J1310027), the Project 111 sponsored by the State Bureau of Foreign Experts and Ministry of Education (B12001), the National Natural Science Foundation of China (31601132) to T.Z., the National Natural Science Foundation of China (81402290) to Q.L., and the National Natural Science Foundation of China (U1405223) to X.D