Study on Tissue Culture and Mechanisms of Adventitious Branches Induction and Inorganic Carbon Acquisition in Gracilaria lichenoides

大型海藻组织培养（seaweedtissueculture，STC）技术可以解决大型海藻栽培中的诸多问题，主要包括高品质藻种的保存、大规模育苗生产以及基因工程研究等。然而，STC还面临着很多困难，例如：愈伤组织（callus）诱导与再分化困难、且形成率低；对于很多藻种，切块培养后会快速极性再生不定芽（adventitiousbranches，ABs）而非callus；同时，这一过程会因藻种的不同而对外源植物激素的敏感性有所不同。研究表明，转录因子、MicroRNA（miRNA）和内源植物激素等因素在细胞脱分化与再分化、植物极性建成以及抗逆机制等生物过程中扮演着重要角色。而正是由于STC缺乏这方...SeaweedTissue culture (STC) could solve the problems associated with Gracilaria cultivation, including the consistent supply of high-quality seed stock, strain improvement, and genetic engering. However, STC lags behind that of higher plants because of the paucity of genomic information. The main morphological response to wounding of seaweed explants in vitro is the direct production of adventitio...学位：工学博士院系专业：化学化工学院_生物化工学号：2062013015380

Study of the transgenic cyanophyta without antibiotic resistance markers

蓝藻是一类具有广泛适应性的光能自养型的原核生物，其所含营养丰富，是人类未来理想的食品；同时，蓝藻作为基因工程受体具有许多优点；但转基因产品中的标记基因的存在是多余的，甚至是有害的，因此要培育具有安全标记基因或者去除选择标记基因的转基因产物；而增强型绿色荧光蛋白（EGFP）被证明是一种安全标记基因，因此本研究以安全标记基因EGFP为标记基因，去除抗生素标记基因，并与流式细胞技术（FACS）相结合，开发无抗性标记的转基因蓝藻。 本次研究的第一部分，设计引物用PCR技术扩增得到钝顶螺旋藻（Spirulinaplatensis）cpcB基因的上游启动片段PcpcB，将此片段插入载体pEGFP中的5＇...Blue green algae are photoautotrophic organisms with many special properties, it has abundant nutrition; Meanwhile, blue green algae have unique nature on cell's organization、metabolizability、heredity, etc., and it has a lot of advantages as genetic engineering receptor. However the existence of the marker genes in the transgenic production are surplus, even harmful, Enhanced Green Fluorescent Pro...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_水生生物学学号：20042602

Design and Simulation of 6 DOF Platform System Based on Virtual Reality

在运载器运动控制领域，运动仿真平台的出现对运动控制技术的发展起着举足轻重的作用。作为模拟运载器运动的机构，运动仿真平台有着广泛的应用：(1)可以作为航空飞行模拟器，(2)可以作为机器人的模拟运动机构，(3)在娱乐界可以作为体感模拟娱乐机，(4)用作飞机、船舶、潜艇、航天器等运动载体中相关仪器设备的试验。Stewart平台机构是运动仿真平台中最著名的一种并联机构平台，Stewart平台的出现始于1965年德国学者Stewart发明的具有六自由度运动能力的并联机构飞行模拟器。目前经典的Stewart平台机构由上、下两个平台和六个可伸缩的支腿以及它们之间的连接铰链构成，其下平台通常为基台（Base-...In the field of motion control, motion simulating platform plays a significant role to improve the development of motion control technology. As a structure for simulating motions, motion simulating platform has various applications, including: simulating the flying simulator, being the simulating structure of robots, simulating the entertaining machine and doing experiments in motion carriers such...学位：工学硕士院系专业：计算机与信息工程学院自动化系_控制理论与控制工程学号：20033100

Research progress on the objectification, quantitation and standardization of tongue manifestation in traditional Chinese medicine

舌诊是中医诊断学的重要组成部分,舌象在中医临床治疗中具有重要的诊断意义与辨证价值。传统中医舌象易受主客观因素影响,难以客观化、定量化、标准化,严; 重制约舌象临床应用及发展。随着医学、计算机等现代技术的迅猛发展,中医舌象客观量化研究取得了长足进步,并被应用于基础及临床研究。文章对近几年中医舌; 象采集分析系统、舌象定量化研究、现代仪器应用与舌象基础研究进行概述,为舌象应用于临床治疗提供客观、科学的思路与方法。Tongue diagnosis is an important part of traditional Chinese medicine; (TCM), and the tongue manifestation is also important in the diagnosis; and syndrome differentiation in TCM clinical treatment. However, due to; the lack of objective, quantitative and standardized valuation, with it; being susceptible to subjective and objective factors, the application; and development of traditional tongue diagnosis is limited for a long; period. Recently, with the rapid development of medical and other modern; technology, the objectification, quantitation and standardization of TCM; tongue manifestation have been received great progress, including the; progress in basic and clinical researches. Recent researches on the; tongue image acquisition and analysis system, the quantitation of tongue; manifestation, the application of modern instruments and the basic; research in tongue manifestation were reviewed to provide certain; objective and scientific ideas and methods for TCM tongue manifestation.教育部新世纪优秀人才支持计划; 中国博士后科学基金面上项

开放式基金,大众理财的首选

开放式基金是投资基金一个主要品种，指发行总额不固定，基金单位总数随时增减，投资者可按基金报价在国家规定的营业场所申购或者赎回基金单位的投资基金。由于开放式基金的某些特点，所以它广受投资者的欢迎。本文将着重向投资者介绍开放式基金的理财特点以及如何投资于开放式基金

虫药验方治疗骨性关节炎之痹证

骨性关节炎为临床的常见病,随着对骨性关节炎认识的不断深入,临床上更注重中药的治疗。但是骨性关节炎的延绵疼痛等一系列症状,采用常规的辨证方法不能起到很好的疗效。可见其发病的机制不简单。通过临床实践发现,骨性关节炎属于久痹、顽痹的范畴。久痹、顽痹属肝脾肾三脏受损所致,此症虽然亦为肝脾肾三脏受损,但其病变的中心却在于肝,本质在于肾精亏虚。精虚则木强,木强亦谓之疏泄过。故采用蜈蚣5条,全蝎15g,穿山甲20g,僵蚕30g,蛇蜕30g,乳香30g,没药30g,上为细末,以草乌30g煎汤,以潜镇息风、祛风息风、化痰解瘀是本病的治本之道,应贯穿本病治疗始终。临证应根据矛盾主次之间进行辨证论治,提高临床疗效。国家自然科学基金面上项目(No.81373999)~

Anti-demodecidosis activity and skin safety ofJatropha curcas.1 leaves extract in vitro

目的 探讨麻风树叶治疗蠕形螨病的应用价值。方法 用80%乙醇热回流提取法提取麻风树叶提取液。取蠕形螨感染者面部皮脂,分离并鉴定蠕形螨备用。设不同浓度麻风树叶实验组、2%浓度的甲硝唑对照组和生理盐水对照组,进行体外抗螨实验。pH仪测定不同浓度麻风树叶提取物pH值。设麻风树叶实验组和75%乙醇对照组,用健康家兔进行皮肤刺激实验和急性皮肤毒性实验。结果 50、25、12 mg/ml麻风树叶组与2%甲硝唑组毛囊蠕形螨死亡时间分别为（1.55±0.67）min、（1.61±0.67）min、（2.47±0.80）min和（1.20±0.48）min。50、25 mg/ml麻风树叶组以及2%甲硝唑组两两之间差异无统计学意义（P均〉0.05）。50、25、12 mg/ml麻风树叶提取液pH值分别为6.07±0.73、6.27±0.82、6.35±0.83,对家兔完整皮肤及破损皮肤刺激评分均为0,且无明显毒性。结论 麻风树叶提取物具有较强的体外抗蠕形螨活性且具有皮肤安全性。Objective To test the application value of the extract of Jatropha curcas.l leaves in treatment of demodecidosis. Methods Jatropha curcas.1 leaves were extracted with 80% ethanol by using heat reflux method. Facial sebum specimen from demodecidosis-infected patients were used to isolate and identify demodecidosis. Different concentration groups of Jatropha curcas.l leaves, 2% metronidazole control group and physiological saline control group were defined. The anti-demodecidosis experiment was performed in vitro. The pH value of different concentrations of Jatropha curcas.l leaves extract was determined. Skin irritation test and acute skin toxicity test were carried out in healthy rabbits, and Jatropha curcas.1 leaves and 75% ethanol served as experiment and control groups, respectivelyl Results The mite-killing time was （1.55-4-0.67） min with 50 mg/ml Jatropha curcas.l leaves extract, （1.61-4-0.67） min with 25 mg/ml Jatropha curcas.l leaves extract, （2.47-4-0.80） min with 12 mg/ml Jatropha curcas.l leaves extract, and （1.20-4-0.48） min with 2% metronidazole. There was no significant different between 50, 25 mg/ml Jatropha curcas.l leaves extract groups and 2% metronidazole group （P 〉 0.05）. The pH value was 6.07＋0.73 of 50 mg/ml Jatropha curcas.l leaves extract, 6.27-1-0.82 of 25 mg/ml Jatropha curcas.l leaves extract and 6.35-4-0.83 of 12 mg/ml Jatropha curcas.1 leaves extract. Score for irritation to normal and wounded rabbit skin was both 0, and acute toxicity test showed no significant toxicity. Conclusions Jatropha curcas.l leaves extract shows a remarkable activity to demodecidosis with skin safety in vitro.漳州市自然科学基金（ZZ2014J33

月湖底泥疏浚后底栖动物群落的恢复及其与环境的关系

研究对月湖底泥疏浚后底栖动物群落动态进行逐月的周年调查,并分析了底栖动物密度、生物多样性与环境因子的关系,结果表明:疏浚导致大型底栖动物基本消失,现存量从疏浚前的(4387±885)ind·m-2降低至(80±21)ind·m-2。疏浚后,寡毛类成为受干扰系统恢复过程中的先锋种类,在春季(4月)和秋冬之际(11月)出现2个密度高峰,分别为(1010±230)ind·m-2和(1538±408)ind·m-2,而摇蚊幼虫在疏浚一年后的秋冬季密度达到高峰(2021±612)ind·m-2,二者均基本恢复到疏浚前的密度水平。种类组成与疏浚前相似,优势种类数较疏浚前多,7月份以前,以霍甫水丝蚓占绝对优势,7月份以后以长足摇蚊占绝对优势。生物多样性在秋冬季恢复到疏浚前水平。寡毛类、摇蚊幼虫的密度、生物多样性与湖水溶氧、透明度呈显著正相关,与水体营养水平(TN、TP、有机碎屑)呈显著负相关(P<0.05)。分析认为疏浚后底栖动物群落的季节变化与动物的生命周期(繁殖和生长)密切相关,而营养水平不是限制动物种群密度分布的主要因子。从底栖动物群落的恢复情况来看,疏浚后的底质环境更有利于底栖动物群落的生存和底栖生态系统的重建

我省发行地方债券势在必行

一、我省发行地方政府债券的客观依据首先,发行地方政府债券是解决我省建设资金不足的有效途径。国家财政近年来给福建追加了不少投资,有力地支援了我省的经济建设,但目前国家财力有限,不可能拨款过多,无法满足我省经济发展需要;银行信贷近年来筹集了大量建设资金,但银行的长期资金来源也很有限,而在目前总需求大于总供给的条件下,银行信贷还面临紧缩的任务,不可能拿出更多的钱来支援本省建设;地方企业和乡镇企业近年来随着开放搞活,自筹资金、发行股票、债券,虽然解决了部分资金需要,但数量有限,加之一些基