--1H-NMR Spectroscopy-based Metabonomic Research on Serum of Model Rats of Wilson's Disease

目的:以基于核磁共振(nMr)的代谢组学方法对WIlSOn病大鼠模型及正常对照组大鼠血清进行研究,分析血清中小分子代谢物的变化,从小分子代谢物层面上探讨WIlSOn病的内在机制,以更加清楚的认识本病。方法:22只雄性WISTAr大鼠,体重(180±20)g,随机被分为模型组(n=11)和健康对照组(n=11),采用铜负荷法制作WIlSOn病大鼠模型,以核磁共振(nMr)技术对大鼠血清进行检测。采用MESTrE-C 2.3软件及自编软件对谱图进行手动调相、基线校正和谱峰对齐。对样品进行分段积分,将积分数据归一化后构成数据矩阵,并利用PCA方法对数据矩阵进行统计分析。结果:相对于正常对照组,模型组大鼠血清甜菜碱(bETAInE)、氧化三甲胺(TAMO)、低密度脂蛋白(ldl)、极低密度脂蛋白(Vldl)、葡萄糖(gluCOSE)含量有显著降低,胆碱(CHOlInE)、胆碱磷酸(PHOSPHOrylCHOlInE)的含量有所降低,乳酸(lACTATE)、谷氨酰胺(gluTAMInE)、糖蛋白(glyCOPrOTEIn)有显著升高,肌氨酸+肌氨酸酐(CrEATInE+CrEATInInE),精氨酸(ArgInInE)有所升高。这些发生改变的代谢物可以作为Wd的小分子代谢标志物,为进一步研究Wd的内在代谢机制提供参考。Objective:Applying 1H nuclear magnetic resonance(1H-NMR)spectroscopy-based metabonomic approach to investigate the changes of small molecular metabolites in the serum from the rats of the model group of Wilson's disease contrasted with those of the control group.Exploring the pathogenesis of Wilson's disease from small molecular aspect.Methods:22 male Wistar rats[weight=(180±20)g]were divided into two groups randomly,the model group(n=11)and the control group(n=11),with the models established with excessive copper method.The serum was tested with 1H-NMR technology.The spectra were edited with MestRe-C2.3 and self-programmed software and then principal component analysis(PCA)was applied to differentiate the two groups.Results:Choline and phosphorylcholine concentrations were found to be lower and TAMO+betaine,LDL,VLDL and glucose were significantly lower in the serum of the model group.While creatinine and arginine concentrations were found to be higher and lactate,glutamine and glycoprotein were significantly higher in the model group.The small molecular metabolites above may contribute to the discrimination,and serve as references for further research on WD pathogenesis.“十一五”国家科技支撑计划分课题重大疑难疾病中医防治研究项目(2006BA104A02

Dynamic simulation of gas hydrate reservoirs in the Shenhu area,the northern South China Sea

为进一步了解南海北部神狐海域天然气水合物的成藏匹配条件,利用典型二维地震剖面,构建了该区的地质模型,并对其进行了天然气水合物成藏动力学的模拟。研究结果表明:神狐海域具备天然气水合物成藏的温度、压力条件;生物气和热解气的资源潜力巨大,满足水合物形成的气源条件;运移条件优越,有利于天然气水合物的聚集成藏。并提出了该区天然气水合物的成藏模式。The north slope of the South China Sea is structurally characterized by a passive continental margin and has various Cenozoic oil-and gas-bearing depositional basins,where hydrocarbon resources are abundant,and the geological and tectonic settings,the temperature and pressure regimes as well as the methane-generative potential of thick organic-rich sediments are all favorable for the formation of gas hydrate.As a result,this region is also an ideal area for studying gas hydrate accumulations.In order to understand natural conditions of the gas hydrate formation,based on the typical 2-dimensional seismic image of the Shenhu area,a geological model of gas hydrate reservoirs was established and a dynamic simulation of gas hydrate accumulations was carried out as well.The results indicated that the temperature and pressure of the Shenhu area are appropriate for gas hydrate accumulations,source rocks there have a huge gas-generative potential and hydrocarbon migration conditions are favorable,providing an abundant gas source and good pathways for the gas hydrate formation.A forecasting model of the gas hydrate formation was given based on basin analysis,indicating that the Shenhu area is a better prospect area of gas hydrate because of its huge potential of gas hydrate resources.国土资源部公益性行业科研专项(200811014)“天然气水合物成藏的控制因素研究”;国家自然科学基金项目(No.40930845)“南海北部深水盆地油气渗漏系统与天然气水合物富集机制研究”;国家重点基础研究发展规划(973)项目(2009CB219501)“南海北部天然气水合物成藏的气源条件研究”联合资

In-situ Coupling Technology of Adsorptive Desulfurization and Biodesulfurization

吸附脱硫和生物脱硫技术具有条件温和、操作简单和成本低的优点，被认为是最具有应用前景的柴油深度脱硫技术。吸附-生物原位耦合脱硫工艺是耦合了吸附脱硫的速率快和生物脱硫的选择性高的优点的新型工艺。该耦合工艺是通过在微生物脱硫细胞上吸附纳米脱硫吸附剂来实现的。本文在模拟体系中对吸附-微生物催化原位耦合脱硫工艺进行了研究。 首先，考察了常用脱硫吸附剂&gamma;-Al2O3，Na-Y分子筛和活性炭在原位耦合脱硫工艺中的脱硫效果。结果表明，Na-Y分子筛和活性炭均不能与德氏假单胞杆菌R-8进行耦合脱硫。纳米结构的&gamma;-Al2O3能够有效地吸附到细胞表面，快速地从油相中吸附DBT，传递给细菌进行生物降解，从而提高脱硫速率。 其次，优化了纳米&gamma;-Al2O3的合成工艺和纳米&gamma;-Al2O3的原位耦合脱硫工艺。分别用溶胶法和过氧化氢法制备得到了纳米&gamma;-Al2O3，其中过氧化氢法制备的&gamma;-Al2O3吸附性能更好。在过氧化氢法制备过程中，经过共沸蒸馏过程得到的&gamma;-Al2O3吸附能力和与德氏假单胞杆菌R-8耦合脱硫速率分别为经过常规水热处理得到的&gamma;-Al2O3 的1.12倍和1.5倍。在纳米&gamma;-Al2O3与R-8耦合体系中，&gamma;-Al2O3吸附剂的最佳用量在1~2 g Al2O3/ g dry cell之间，最佳脱硫pH范围为5~8。质量为1g的纳米&gamma;-Al2O3与德氏假单胞杆菌R-8耦合能将脱硫速率提高到纯R-8脱硫速率的2.7倍。 为了消除&gamma;- Al2O3纳米颗粒的在水相中的团聚现象，采用阿拉伯胶(GA)对&gamma;- Al2O3纳米颗粒进行了表面修饰。阿拉伯胶通过空间位阻作用，使&gamma;-Al2O3颗粒能在水相中稳定分散。500mg未经修饰的&gamma;-Al2O3与R-8进行耦合脱硫，将脱硫速率提高到了纯R-8脱硫速率的1.23倍，同等质量经过GA修饰的&gamma;-Al2O3能将耦合速率提高到纯R-8脱硫速率的1.77倍。即GA改性能使&gamma;-Al2O3的耦合速率得到提高，可有效减少耦合脱硫中&gamma;-Al2O3的用量。</p

Управление финансовыми рисками организации : выпускная бакалаврская работа по направлению подготовки: 38.03.01 - Экономика

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运用大涡模拟对超音速氧碘化学激光器内混合过程的优化研究

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普适计算环境下的设备请求代理模型

为了给普适计算环境提供一个统一的应用架构和模式,从普适计算研究现状出发,通过借鉴对象请求代理技术提出了设备请求代理模型,建立了设备基件的远程映射管理和统一调度机制,有效降低了普适计算环境下应用程序的开发难度和兼容传统应用程序

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目前泛在设备接口不统一,并且大都不具备互感知和互操作能力。本文通过接口抽象技术提供设备间的统一操作接口,从固件层上解决了泛在设备间的互感知和互操作问题,并成功应用到智能家居系统中。该系统具有较强的扩展性和可靠性,应用成本较低。基金申请人:史兴国|目名称:泛在设备的固件通用接口|基金颁发部门:国家863计划(2009AA011901