磁共振胰腺脂肪定量测定预测糖耐量异常及2型糖尿病

目的探讨磁共振胰腺的脂肪含量测定预测糖耐量异常（IGT）及2型糖尿病（T2DM）的可行性。方法108名受试者（43男，65女；年龄47.9±12.1岁），包括T2DM（n=27），IGT（n=29）和葡萄糖耐量正常者（NGT；n=53）。NGT组进一步分为<40岁的NGT-年轻组和 ≥40岁的NGT-年长组。所有受试者均进行针对胰岛素抵抗和β细胞功能障碍的标准实验室检测。利用MRI非对称回波三点法水脂分离-定量（IDEAL-IQ）技术测定胰腺脂肪含量。分析胰腺脂肪体积分数（PFVF）与实验室检测参数之间的相关性，并分析PFVF预测IGT和T2DM的可行性。结果T2DM患者的PFVF显著高于其余各组， IGT患者PFVF次之，NGT组PFVF最低。Logistic回归分析提示PFVF是血糖异常（IGT及T2DM）的独立危险因素。ROC曲线分析显示PFVF预测血糖异常（IGT及T2DM）的临界值为5.68%（P < 0.001， AUC：0.871， 敏感度92.7%，特异度71.7%）。结论MRI脂肪定量测定技术可以测定胰腺的脂肪含量，为血糖异常（IGT和T2DM）的预测提供无创性的生物指标

SURVEY OF MIXED INFECTIONS OF FISH-BORNE TREMATODES IN PINYUAN COUNTY AND DNA(RAPD)ANALYSIS

目的　调查广东省平远县棘隙吸虫及其他鱼源性吸虫混合感染情况。方法　对鱼类宿主取鱼鳃和鱼肉等组织 ,镜检囊蚴 ;用水洗过筛沉淀法粪检虫卵 ,部分虫卵阳性动物解剖检查。采用DNA -RAPD技术 ,比较福建棘隙吸虫广东株与福建株。结果　查出华支睾吸虫 (Clonorchissinensis)、东方次睾吸虫 (Metorchisorientalis)、福建棘隙吸虫 (Echinochasmusfujianensis)、日本棘隙吸虫 (E japonicus)、抱茎棘隙吸虫 (E perfoliatus)和钩棘单睾吸虫 (Haplorchispumilio) 6种。第二中间宿主为麦穗鱼等 11种 ,感染率 48 3% (182 / 377) ,有 92 4%的阳性鱼混合感染 2种以上吸虫囊蚴。保虫宿主狗、猫感染率 79 2 % (19/ 2 4) ,每只阳性动物均有 2种以上吸虫混合感染。 15个引物在福建棘隙吸虫广东株与福建株两者共获DNA片段 185个 ,共享度 (F) =0 984,遗传距离 (D) =0 0 16。结论　当地为人兽共患的多种鱼源性吸虫混合感染区。形态学观察和分子生物学检测结果相印证 ,福建棘隙吸虫广东株与福建棘隙吸虫福建株为同一虫种。Aim To seek the possible existence of mixed infections of Echinochasmus fujianensis and other fish-borne tremtodes in Pinyuan county,Guangdong.Methods Metacercariae of trematodes were detected from gills,muscles and some other tissues of freshwater fish and examintd under microscopy Sedimentation pocedures were used to observe parasite ova in stool Adult worms were obtained from definitive animal hosts upon dissection Experimental animal infection were performed from metacercariae of E fujianensis strains in Fujian and Guangdong,and it's DNA analysis Random amplified Polymorphic DNA(RAPD).Results Six species of trematodes were found,including Clonorchis sinensis,Metorchis orientalis,E fujanensis, E japonicus,E perfoliatus and Haplorchis pumilio Eleven species of freshwater fish comprising Pseudorasbora parve were identified as the second intermediate hosts,with an average positive rate of 48 33%(182/377) The overall mixed infection animal were contracted with 2 or more species Dogs and cats were detected to be reservoir hosts,the total infection rate being 79 2%(19/24).92 4% of infecded fish were contracted with 2 or 6 speies of the metacercariae of trematodes 185 DNA fragments were obtained by 15 primers,F=0 894 and D=0 016 between E fujianensis Guandong and Fujian strain Conclusion There are six species of fish-borne trematodes which may cause infections in human and animal in the regions E fujianensis Guangdong stain and Fujian strain are the same species福建省科技计划基金!资助项目 (No 98-Z - 1 56);; 福建省“百千万人才”人选培养资

中国高等植物受威胁物种名录

2008年,环境保护部和中国科学院联合启动了《中国生物多样性红色名录——高等植物卷》的编制工作。通过这项工作,我们依据IUCN濒危物种红色名录标准对中国野生高等植物的濒危状况进行了全面评估,编制了中国高等植物红色名录。2013年9月,该名录以环境保护部、中国科学院第54号公告形式发布,即《中国生物多样性红色名录—