193 research outputs found
A Study on the Seafarer Compensation System for Occupational Hazards in Taiwan and Comparative Reviews of the Related Laws in Mainland of China and Japan
内容摘要 海运带动经济发展,商船船舶为海运经营的主要工具,船员则为船舶营运的尖兵。船员的工作充满了危险,常有意外(包括伤病及死亡)发生,因此船员工作安全和伤病的议题,自始至终都是个不可完全避免的问题,船员的工伤(职业灾害)补偿问题受到关注。本研究之主题为“台湾船员工伤补偿制度研究--兼两岸及日本相关立法比较”,研究之对象为台湾船员,梳理船员各种意外保障措施,阐析当意外发生时,船员享有哪些权益;以国际宏观角度审视台湾地区的补偿措施是否跟得上国际水平;也比较两岸与日本的工伤(职业灾害)补偿制度,俾了解台湾船员在伤病与抚恤方面的保障是否足够,并提出立法与实践之完善建议。 全文除绪论和结语外,正文...ABSTRACT Ocean shipping drives the development of economy. The merchant ship is the major facility in the operation of ocean shipping, and the crew is the key of ship operation. However, the job of crew is filled with risks usually, and the accidents including diseases and death occur frequently. Therefore, the issues regarding the safety of crew job and disease are unavoidable questions, and the...学位:法学博士院系专业:法学院_民商法学学号:1362011015446
硝基芳烃类污染物对水生态系统的毒理研究述评
硝基芳烃主要通过废水、粉尘、蒸气等形式污染环境,影响人体健康.从其对水生生物(包括生产者、消费者和分解者)的形态结构、生理生化、分子机制和对水生态系统的影响等方面综述了它们的生态毒害和致毒机理
Fabrication and Characterization of Pt Catalyst on PANI/Nano Carbons Composite Carriers with High Surface Activity
科技与经济的高速发展让能源危机和环境污染问题日益受重视,燃料电池作为“二十一世纪的清洁能源”日益成为关注和研究的焦点。直接甲醇燃料电池(DMFC)以甲醇作为燃料,具有来源丰富、价格便宜、在常温下是液体、易携带和储存且能量密度高等优点,将成为比氢燃料电池更具有吸引力的一类质子交换膜燃料电池(PEMFC)。阳极催化剂是DMFC关键的组成部分。目前铂(Pt)是DMFC体系中最适的催化剂,但利用效率低和CO中毒是主要问题。针对这些问题,本论文通过制备出具有高表面活性的聚苯胺/纳米碳复合材料,以之作为铂金属载体,使金属粒子在载体表面呈均匀的分散状态,提高了Pt催化剂的利用率;Pt粒子与聚苯胺之间存在的协...With the rapid development of technology and economy, the energy crisis and environmental problems are received increasing attention. Fuel cell as the "Clean Energy In the 21st Century" is increasingly becoming a concern and research focus. Direct methanol fuel cell (DMFC),using methanol as fuel, being abundant sources, low prices, liquids at room temperature, easy to carry and store and higher en...学位:理学硕士院系专业:化学化工学院材料科学与工程系_高分子化学与物理学号:20043600
Nitrification Performance of Saline Sewage Treatment Using Biofilter Packed with Oyster Shell
随着经济的迅速发展,人们对水资源的利用量越来越大。然而,目前世界各地的淡水资源告缺,因而探索和利用新水源成为全世界亟待解决的问题。占世界总水量96.5%的海水,若能被用于日常生活,将极大地缓解人们的用水紧张问题。但由于海水的独特性质,使得海水污水的处理和处置存在一些问题,若能将这些问题解决,则为海水的利用提供了可能。 本研究采用曝气生物滤池工艺,以牡蛎壳填料为生物载体,含盐生活污水与含海水污水为处理对象,在SBR运行条件下,系统考察了进水盐度、曝气时间、水力停留时间以及进水pH等操作因子对该工艺硝化性能的影响。 具体的研究结果归纳如下: (1)生活污水中的NaCl浓度从2g/L逐渐增大到...With the rapid development of the economy, the utilization of water resource is increasing. However, the shortage of fresh water resource is worldwide currently. Therefore, how to develop an alternative water resource has become an urgent task worldwide. Seawater accounts for the most of all water resources, so the problem of water supply could be greatly alleviated if seawater is used in our dail...学位:工学硕士院系专业:海洋与环境学院环境科学与工程系_环境工程学号:2262009115117
拟穴青蟹抗菌肽Crustin新变体的表达特性与抗菌功能
Crustins是一类较早发现并广泛分布在甲壳动物中且富含半胱氨酸的阳离子抗菌肽,能够参与抗细菌的免疫应答。从拟穴青蟹(Scylla paramamosain)中克隆获得Crustin基因,命名为SpCrus1a,其cDNA序列全长744 bp,开放阅读框编码113个氨基酸,成熟肽分子量10.03 kDa,等电点8.30。表达分析结果发现其转录本主要存在于鳃、卵巢、上皮组织中,脂多糖刺激后SpCrus1a会上调表达。体外合成SpCrus1a的第29~47位氨基酸进行抗菌活性实验发现,其对革兰氏阳性菌具有较强的抗菌活性,对被测的革兰氏阴性菌抗菌活性较弱或无抗菌活性。合成肽Sp Crus1a29-47能够在较短时间内杀死绝大多数的金黄色葡萄球菌,2 h内杀死全部的金黄色葡萄球菌。扫描电镜发现合成肽Sp Crus1a29-47可造成金黄色葡萄球菌表面结构崎岖不平,高浓度Sp Crus1a29-47造成细菌的大量死亡。上述结果表明,SpCrus1a是抗菌肽Crustins的新变体。国家自然科学基金(U205123,31172438);;厦门南方海洋研究中心科技项目(14PYY050SF03
Nitrification of saline sewage using oyster shell packing biofilter
采用牡蛎壳为曝气生物滤池填料,以含nACl的生活污水为处理对象,在Sbr操作条件下,系统考察进水nACl浓度、曝气时间及进水PH值等对硝化性能的影响。结果表明,进水nACl浓度为10~15 g/l时,平均氨氮去除率可稳定在97%以上;较高浓度nACl对亚硝酸化菌活性影响较弱,对硝酸化菌活性影响较强,特别是在日曝气时间少于12 H时,其出水中亚硝氮的含率大于50%;当进水PH值在6~9变化时,反应器内PH值可稳定在6.5~7.5,硝化性能良好,表明牡蛎壳填料可为硝化反应提供碱度。The performance on which oyster shell packing biofilter removed ammonia from saline sewage was investigated using different parameters,such as influent NaCl concentration,aeration time and pH.In sequencing batch experiments,97 percent of ammonia in sewage was removed at concentrations of NaCl ranging from 10 to 15 g/L,indicating that ammonia oxidizing bacteria could tolerate a high salinity.In contrast,nitrite oxidizing bacteria exhibited sensitive to this salinity,resulting in nitrite accumulation.Over 50 percent of nitrite accumulation rate was observed at aeration time of less than 12 h.In the influent pH of 6 ~ 9,nitrification could proceed successfully at a favourable pH of 6.5 ~ 7.5,suggesting that oyster shell played a very important role in alkalinity supply.深圳市科技研发资金资助项目(JCYJ20120830153030587); 深圳虚拟大学园专项经费资助项目(2012
Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells
AIM: To investigate the effects of tachyplesin on the cell cycle regulation in human hepatcarcinoma cells. METHODS: Effects of tachyplesin on the cell cycle in human hepatocarcinoma SMMC-7721 cells were assayed with flow cytometry. The protein levels of p53, p16, cyclin D1 and CDK4 were assayed by immunocytochemistry. The mRNA levels of p21(WAF1/CIP1) and c-myc genes were examined with in situ hybridization assay. RESULTS: After tachyplesin treatment, the cell cycle arrested at G(0)/G(1) phase, the protein levels of mutant p53, cyclin D1 and CDK4 and the mRNA level of c-myc gene a were decreased, whereas the levels of p16 protein and p21(WAF1/CIP1) mRNA increased. CONCLUSION: Tachyplesin might arrest the cell at G(0)/G(1) phase by upregulating the levels of p16 protein and p21(WAF1/CIP1) mRNA and downregulating the levels of mutant p53, cyclin D1 and CDK4 proteins and c-myc mRNA, and induce the differentiation of human hepatocacinoma cells
Effects of tachyplesin on proliferation and differentiation of human hepatocellular carcinoma SMMC-7721 cells
AIM: To investigate the antitumor activities of tachyplesin on human hepatocellular carcinoma (HCC) cells. METHODS: Tachyplesin, isolated from acid extracts of Chinese horseshoe crab ( Tachypleus tridentatus) hemocytes, was used to treat the human HCC cell line SMMC-7721. Effects of tachyplesin on the proliferation of SMMC-7721 cells were measured with trypan blue dye exclusion test and HE staining. The morphology and ultrastructure of the cells were examined by light microscopy and transmission electron microscopy, respectively. The activities of gamma-glutamyltransferase (gamma-GT) and tyrosine aminotransferase (TAT) were assayed with biochemical methods. The levels of alpha fetoprotein (alpha-FP), proliferating cell nuclear antigen (PCNA), p21(WAF1/CIP1) and c-myc were examined by immunocytochemistry. RESULTS: After treatment with tachyplesin 3.0 mg/L, the proliferation of SMMC-7721 cells was inhibited significantly, with the cell growth inhibitory rate amounted to 55.57% and the maximum cell mitotic index declined by 43.68%. The morphology and ultrastructure underwent restorational alteration. The activity of gamma-GT declined while TAT activity increased obviously, and the levels of alpha-FP and PCNA decreased. Moreover, the expression of p21(WAF1/CIP1) protein was up-regulated and that of c-myc protein was down-regulated. CONCLUSION: Tachyplesin could effectively inhibit the proliferation of hepatocarcinoma cells, reverse the malignant morphological and ultrastructural characteristics, alter the levels of enzymes and antigens, regulate the expression of differentiation-associated oncogene and tumor suppressor gene, and induce hepatocarcinama cell differentiation
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