Study on the government's role and exit mechanism in the investment and operation of the large convention center

会展业的发展对世界经济起到了很好的助推作用，会展场馆作为重要的物质载体，一直被誉为会展经济发展的火车头。社会的进步，新技术的不断应用与提高，也使得会展场馆的设施和功能日趋多样化，会展场馆的经营管理受到越来越多的重视。目前，业界普遍认为，由于会展场馆，特别是大型会展场馆的公益性特征，政府应该是会展场馆的唯一投资方，大型会展场馆的投资运营都应该由政府来发挥其主导作用。而同时，随着会展业的国际化发展战略，大型会展场馆的投资运营出现了市场化运作趋向，政府开始逐渐退出对大型会展场馆的投资和运营。在这种背景下，政府在会展场馆，尤其是大型会展场馆中的角色应如何思考和定位，政府的退出方法与退出后的职能定位成为...The development of the exhibition industry has played a very good thrusting role to economy. Convention centers, as the most important material carrier of the exhibition's economic development, has been known as the locomotive of the exhibition economy. The progress of the society and the continuous application of new technologies also make the facilities and functions of convention centers become...学位：旅游管理硕士院系专业：管理学院旅游系_旅游管理学号：1782010115432

Loop-Mediated Isothermal Amplification(LAMP) for Detection of Alicyclobacillus acidoterrestris in Foods

目的:利用环介导等温扩增技术建立食品中酸土环脂芽孢杆菌快速检测方法。方法:针对酸土环脂芽孢杆菌16S序列设计特异引物,再优选反应体系,用显色法检测实验结果。结果:该方法能够在63℃条件下1 H内检出食品中酸土环脂芽孢杆菌,所设计的引物有良好的特异性;灵敏度达6.7 Cfu/M l(弱阳性)。结论:该方法具有高效、特异性强和敏感性高等特点,可满足酸土环脂芽孢杆菌快速检测筛选的要求。Purpose: A loop-mediated isothermal amplification(LAMP) method was established for the detection of Alicyclobacillus acidoterrestris in foods.Methods: After optimization of the reaction conditions of LAMP including the concentrations of primers, reaction time and amplification temperature, the LAMP method was developed, and its sensitivity and specificity were evaluated.Results: The method was capable of rapidly and specifically detecting A.acidoterrestris in foods within 1 hour at a constant temperature of 63 ℃.The sensitivity of the method was 6.7 CFU/m L and the specificity was 100%.Conclusions: The LAMP method is efficient, highly sensitive and specific, and suitable for the rapid detection of A.acidoterrestris in various food samples.福建省漳州市自然科学基金项目(ZZ2012J16