Physio-biochemical effects and mechanisms of acidified seawater on several copepods

由于海水对CO2的吸收以及海洋封存CO2导致的海水酸化已经成为全球性的环境问题，其不仅引起海水化学性质的改变，而且对海洋生物的生存、生长和繁殖产生影响。浮游动物在海洋物质循环和能量流动中占有重要地位，研究浮游动物对海水酸化的响应将有助于我们更好的理解海水酸化对生物产生的影响。本研究选取中华哲水蚤、太平洋纺锤水蚤、刺尾纺锤水蚤、瘦尾胸刺水蚤等几种桡足类作为研究对象，比较CO2酸化和HCl酸化对桡足类的24h和48h的急性毒性(LC50pH)，并分析二者之间的差异；采用实验生态学的方法，研究了为期8d不同酸化水平(pCO2800、2000、5000和10000ppm)对桡足类存活率、产卵率以及卵孵...Ocean acidification induced by absorbing CO2 or sequestration CO2 into the deep-sea has become a serious environmental issue. It’s not only changed the seawater chemical characteristic, but also impacted the survival, growth and reproductive performance of marine organisms. Zooplankton, as one of the most important parts of marine organisms, plays an indispensable role during the marine material c...学位：理学博士院系专业：海洋与环境学院海洋学系_海洋生物学学号：2242007015357

design of requirements negotiation tool based on winwin theory

WinWin理论提出项目成功必须让项目的所有涉众达到共赢.实践证明Easywinwin,ARENA,ARENA-M这些基于Win-Win理论的协商工具在获取最初需求时非常有效,然而它们在支持需求的扩展,涉众特别是用户共享知识以及随着项目的进行更新赢条件及共识有一定局限性.WikiWinWin是基于JspWiki引擎设计开发的需求协商工具.这个工具的主要优势在于加强了涉众交流和共享知识,能够很有效地支持WinWin需求协商模型.在设计方面,JspWikiWinWin有效利用JspWiki的插件机制完成了对WinWin协商模型的支持

An Evolutionary Approximation Algorithm for Strip Rectangular Packing Problem

提出了利用近似算法求解二维矩形装箱问题的最小高度的一种方法。该方法基于启发式递归策略和遗传算法。利用启发式递归策略把所有大小各异的矩形都装入宽度固定的矩形容器中,并计算装完后所需容器的高度,用遗传算法的进化能力优化高度,使得所需容器的高度尽可能小。计算数据证明这种方法能够得到很好的结果,特别是对数据量大的测试问题,效果更好。An evolutionary approximation algorithm to find a minimum height for two-dimensional strip rectangular packing problem is presented. This method is mainly based on heuristic strategies and genetic algorithm.The heuristic strategies are used to calculate the height of the packing order,and then the evolutionary capability of genetic algorithm to reduce the height is used.The computational results on well-known benchmark problems show that the presented approximation algorithm can compete with other algorithms based on genetic algorithm.Especially for large test problems,it performs better.厦门大学院士启动基金资助项目(XX01109

Impacts of CO_2-driven acidified seawater on the muscle and oocyte ultrastructure of two marine copepods

利用实验生态学方法及透射电子显微镜技术对培养于CO2酸化海水中的中华哲水蚤和刺尾纺锤水蚤肌肉和卵母细胞超微结构进行了观察。结果表明:两种桡足类肌丝排列的有序性均随酸化的加剧而降低,较高CO2浓度海水会对桡足类产生一定程度的氧化损伤;桡足类卵母细胞的电子密度随酸化的加剧而降低,球形颗粒出现内缩或瓦解,酸化可能会导致桡足类卵母细胞发生过量凋亡,从而影响卵的质量。The muscle and oocyte ultrastructure of Calanus sinicus and Acartia spinicauda which exposed to CO2-driven acidified seawater in laboratory-controlled were observed under the transmission electron microscopy.The results showed that the orderly of myoneme of these two copepods were decreased with the concentration of CO2 increased.The higher cCO2(0.20%,0.50% and 1.00%) had oxidative damage on the copepods.The electron-dense of oocytes of copepods were decreased with the increased cCO2.The spherical granule were concentrated or collapsed.The elevated cCO2 could induce the apoptosis and impact the quality of eggs of copepods.中国二氧化碳海底封存能力评估与风险控制技术预研究(200805029

一种绳驱动并联康复机器人空间安全墙分析及回归轨迹规划方法

在步态康复训练期间，偏瘫或中风患者容易发生肌肉痉挛，从而导致不能支撑自身而失去平衡，造成二次伤害。针对上述问题，设计了一种由8根绳索驱动的6自由度并联康复机器人，不仅能对患者进行步态训练，还能对患者实施有效的防跌倒保护。首先，对机器人构型进行描述，并利用封闭矢量四边形法建立其运动学模型；其次，结合绳索驱动的特性，依据静力学分析，提出一种机器人力控工作空间的概念及其求解方法。为了避免患者失去平衡后受到二次伤害，基于力控工作空间，进一步提出了一种空间轨迹安全墙，对患者可能会出现的待保护位形进行了分类，并分别研究了其回归安全墙内的空间轨迹路径规划方法。最后，选取了两类不同的待保护位形，研究其沿回归轨迹运动时绳索长度和绳索拉力的变化情况，验证了回归安全墙轨迹规划方法的有效性和合理性

Proteomic study of the effects of acidified seawater on Calanus sinicus

运用蛋白质组学相关技术对暴露于酸化海水中的中华哲水蚤全蛋白进行分析,结果表明,对照组、CCO20.08%和CCO20.20%处理组中华哲水蚤的双向电泳图谱上可以分别分辨出1 191、1 117和946个蛋白点,选取其中43个差异蛋白进行MAldI-TOf/TOf质谱鉴定,成功鉴定出23个差异蛋白,这些蛋白主要与蛋白合成和分解、能量代谢、dnA分子修复以及解毒过程有关。This study compared proteim profiles of Calanus sinicus cultured under CCO20.08% and CCO20.20%CO2-acidified seawater for 4days using aproteomic approach,and identified the differentially expressed proteins.The results are shown that,1 191,1 117 and 946protein spots of C.sinicusin control,CCO20.08%and CCO20.20%groups were detected in the two-dimensional electrophoresis gels,respectively.The 43 protein spots were selected based on their differential expression between control and CCO20.08%group,CCO20.20%group,and 23 proteins of which were identified by MALDI-Tof/Tof mass spectrometry.The data demonstrated that these differentially expressed proteins were associated with protein synthesize and proteolysis,energy metabolism,DNA repaired and detoxified of organisms.国家自然科学基金“不同生态类型的海洋桡足类对硅藻的利用与适应的生态遗传学研究”面上项目(41276132

Comparative Study on the Acute Toxicity of Ocean Acidification Driven by CO_2 and HCl on Several Marine Copepods

利用实验生态学的方法研究了由二氧化碳和盐酸引起的海水酸化对几种桡足类的急性毒性,计算了24和48 HlC50(以PH值表示).结果表明:二氧化碳酸化条件下,几种桡足类的24和48 HlC50分别为PH 5.85--6.49和PH 5.93--6.69;盐酸酸化条件下,24和48 HlC50分别为PH 5.02--5.69和PH 5.25--6.12.裂区设计方差分析表明,二氧化碳酸化对桡足类的毒性显著高于盐酸酸化的毒性.此外,各种桡足类对海水酸化的耐受性具有高度的种类特异性:营底栖生活的日本虎斑猛水蚤的耐受性明显高于其他浮游性种类;在营浮游性生活的种类中,植食性种类(中华哲水蚤)对酸化的耐受性要高于杂食性和肉食性种类.本研究结果为进一步研究海水酸化对桡足类生理生化影响提供参考依据.The acute toxicity of ocean acidification induced by CO2 or HCl on several marine copepods was investigated by using the experimental ecological method.The results showed that,the 24 h and 48 h LC50 of copepods were pH 5.85 to 6.49 and pH 5.93 to 6.69 in the CO2-driven acidification groups,while were pH 5.02 to 5.69 and pH 5.25 to 6.12 in the HCl-driven groups.The split plot ANOVA indicated that the acute toxicity of CO2-induced acidification on the copepods was significant higher than that of HCl-induced acidification.Furthermore,the sensitivity of copepods to seawater acidification was species-specific.The benthic copepod,Tigriopus japonicus,had higher tolerance to seawater acidification than the planktonic ones,and the herbivorous copepod,Calanus sinicus,had higher tolerance than the omnivorous and carnivorous copepods among the planktonic copepods.The obtained data also provide important reference for the further study on the impacts of ocean acidification on the physiological and biochemical of copepods.海洋公益性行业科研专项项目(200805029

全硅片上光互连用波导

较详细地分析了用于全硅片上光互连所用光波导（如多晶Si／SiO2、Si／SiO2、Si3N4/SiO2）需满足的基本条件、制作方法以及损耗机制，总结了目前的研究进展

甲烷氧化偶联制C_2烃La_2O_3基催化剂的研究

本文的实验结果表明,具有开放型本征构型的La_2O_3对甲烷氧化偶联反应有相当好的活性和选择性,在750℃、原料气空速6.O×10~4ml.g~(-1).h~(-1),CH_4:O_2:N_2=3.7:l.0:9.0(V/V)的反应条件下,氧接近全部转化,甲烷转化率达25.4％,C_2烃选择性为43.6％,添加碱土金属(Ca、Sr、Ba)氧化物或碳酸盐能显著地改善催化剂的性能,在上述反应条件下,60mol％Ba-La_2O_3催化剂的C_2烃选择性达到58％