The association between IgG1,IgG2 subclasses toward human papillomavirus16-like particles in the serum and cervical intraepithelial neoplasia

背景与目的:免疫球蛋白g(IMMunOglObulIn g,Igg)是血清和细胞外液中含量最高的免疫球蛋白,其某个亚类的升高,可能与宫颈上皮内瘤变(CErVICAl InTrAEPITHElIAl nEOPlASIA,CIn)的发生、发展及转归有密切相关。然而对于CIn患者血清人乳头瘤病毒样颗粒(HuMAn PAPIllOMAVIruS VIruS-lIkE PArTIClES,HPV VlPS)Igg1及Igg2抗体变化目前尚不明确。本研究旨在探讨CIn患者血清中HPV16VlPS-Igg1、Igg2亚类抗体的变化及其与不同级别宫颈病变的关系。方法:采用ElISA法检测HPV感染患者HPV感染患者CIn患者及子宫平滑肌瘤或宫颈炎患者血清中HPV16VlPS-Igg和HPV16VlPS-Igg1、Igg2亚类抗体的表达水平。结果:患者血清中HPV16VlPS-Igg和Igg1抗体含量随宫颈病变级别增高而增加,差异有统计学意义(P1),分别为100.00%、87.50%和75.00%,而CInⅡ~Ⅲ组中仅为9.52%,与前3组相比,差异有统计学意义(P1),可能与HPV清除和宫颈病变逆转有关。Background and purpose:The maximum content of immunoglobulin G was found in serum and extracellular fluid.The increase of some immunoglobulin G subclasses may closely correlate with the occurrence,development and the outcome of cervical lesions.However,the changes of IgG subclasses toward human papillomavirus 16-like particles(HPV16VLPs) in the serum of patients are still under investigation.This paper aimed to study this issue and also analyze the relationship between the expression of IgG subclasses and different grades of cervical lesions.Methods:The expression of IgG subclasses in 32 human papillomavirus,30 cervical intraepithelial neoplasia(CINⅠ) ,43 CINⅡ-Ⅲ,24 hysteromyoma and chronic cervicitis were examined by ELISA.Results:The absorbance value of HPV16-IgG,IgG1 increased with the grade of CIN(P1) from control group was 100%,87.50% for HPV infection group,75% for CINⅠ group,compared with that from CINⅡ-Ⅲ patients(9.52%) (P1) in serum from low grade cervical lesions group and normal control group,might indicate the clearance of HPV infection and the regression of cervical lesions