Synthesis of Ru(dpp)_3(ClO_4)_2 doped polyacrylonitrile nanoparticles and its applications in ratiometric pH sensing

采用乳液聚合法制备了掺杂有4,7-二苯基-1,10-邻菲咯啉钌(ru(dPP)3(ClO4)2)的聚丙烯腈纳米颗粒(ru-PAn).经扫描电子显微镜(SEM)表征,制备的ru-PAn的尺寸为135±15 nM,呈规则球型,尺寸分布均匀且在水中的分散性较好.实验考察了氧气、共存离子和PH值对其荧光性质的影响以及其荧光稳定性.以异硫氰根荧光素(fITC)为PH荧光指示剂、ru-PAn为参比信号,初步建立了一种比率荧光PH检测的方法.In this paper Ru(dpp)3(ClO4)2 doped polyacrylonitrile nanoparticles(Ru-PAN) were prepared using emulsion polymerization method.Morphology characterizations of the nanoparticles were performed using scanning electron microscope,the particle diameter was about 135±15 nm.The interference of the oxygen,coexisting ions,pH and photo-stability were investigated.A new ratiometric fluorescence pH sensing system was established via fluorescein isothiocyanate(FITC) as pH-sensitive dye and Ru-PAN as a reference.国家自然科学基金资助项目(20975085

Theory of Double Mass Curves and Its Applications in Hydrology and Meteorology

在水文气象要素一致性及其长期演变趋势研究中,双积累曲线(DMC)方法以简单、直观、实用而被广泛应用。近30年来我国学者应用该方法分析水土保持措施、土地利用变化的水沙效应取得了良好效果。双累积曲线方法的理论基础是分析两个要素(或变量)应具有正比关系。故在应用时要注意分析的要素应该具有相同物理成因或具有明确的因果关系,参考变量应该是不受其他因素影响的、自然变化的正确值,特别是在河流水沙分析中,降雨量需用具有代表性的面平均降雨量

Clinical characteristics of Haemophilus influenzae and Branhamaceae catarrhaliinfections and analysis of drug susceptibility

目的分析厦门地区流感嗜血菌和卡他布兰汉菌的临床特征及其耐药性,为临床治疗提供依据。方法对2008年1月-2012年12月分离580株流感嗜血菌和185株卡他布兰汉菌进行分析,细菌鉴定采用VITEk-2COMPACT全自动分析系统,药敏检测采用ATbTM HAEMO药敏条,β-内酰胺酶检测采用CEfInASE纸片,数据采用WHOnET 5.6软件进行分析。结果流感嗜血菌与卡他布兰汉菌主要来自儿科住院患儿,以≤4岁儿童为主,流感嗜血菌和卡他布兰汉菌的高发季节分别为春季和冬季;流感嗜血菌产β-内酰胺酶率为47.1%、流感嗜血菌对氨苄西林耐药率高达52.1%,对头孢克洛、磺胺甲噁唑/甲氧苄啶、四环素耐药率≥15.0%;流感嗜血菌的产β-内酰胺酶菌株和非产β-内酰胺酶菌株均对环丙沙星、左氧氟沙星、头孢噻肟、阿莫西林/克拉维酸的敏感率≥80.0%;卡他布兰汉菌产β-内酰胺酶率为52.9%,对氨苄西林耐药率为10.8%,其对阿莫西林/克拉维酸、氯霉素、头孢呋辛、头孢噻肟、利福平、头孢克洛、左氧氟沙星、四环素、磺胺甲噁唑/甲氧苄啶的耐药率均≤5.0%。结论流感嗜血菌和卡他布兰汉菌主要来自儿科患儿,检出率受季节变化影响;其对氨苄西林的耐药率较高;流感嗜血菌有较高的β-内酰胺酶检出率,但对临床常用药物阿莫西林/克拉维酸、四环素的敏感性较高。OBJECTIVE To observe the clinical characteristics of Haemophilus influenzae and Branhamaceae catarrhali infections in Xiamen area and analyze the drug resistance so as to provide guidance for clinical treatment.METHODS Totally 580 strains of H.influenzae and 185 strains of B.catarrhali that were isolated from Jan 2008 to Dec 2012 were analyzed,then the bacterial identification was performed by using VITEK-2Compact automatic analyzer,the drug susceptibility testing was performed with the use of ATBTM HAEMO reagent,theβ-lactamase was detected by using Cefinase disk,and the data were statistically analyzed by means of WHONET 5.6software.RESULTS The H.influenzae and B.catarrhali strains were mainly isolated from the hospitalized children in pediatrics department,and the children aged no more than 4years were the major population;H.influenzae and B.catarrhali strains were prevalent in spring and winter.The isolation rate of theβ-lactamase-producing H.influenzae was 47.1%,the drug resistance rate of the H.influenzae to ampicillin was 52.1%,and the drug resistance rates to cefaclor,trimethoprim-sulfamethoxazole,and tetracycline were 15.0% or above;the drug susceptibility rates of both theβ-lactamase-producing H.influenzae strains and the non-β-lactamase-producing H.influenzae strains to ciprofloxacin,levofloxacin,cefotaxime,and amoxicillin-clavulanic acid were no less than80.0%.The isolation rate of theβ-lactamase-producing B.catarrhali strains was 52.9%,the drug resistance rate to ampicillin was 10.8%,and the drug resistance rates to amoxicillin-clavulanic acid,chloramphenicol,cefuroxime,cefotaxime,rifampin,cefaclor,levofloxacin,tetracycline,and trimethoprim-sulfamethoxazole were no more than 5.0%.CONCLUSIONThe H.influenzae and B.catarrhali strains are mainly isolated from the children in pediatrics department,of which the isolation rate are influenced by the season;the drug resistance rates of the strains to ampicillin are high.The isolation rate of theβ-lactamase-producing H.influenzae strains is high,but the strains are highly susceptible to the commonly used antibiotics such as amoxicillin-clavulanic acid and tetracycline.国家自然科学基金资助项目(81000762); 福建省自然科学基金资助项目(2010D018); 福建省卫生厅青年基金资助项目(2010-2-90

应变自组装InAlAs量子点材料和红光量子点激光器

通过提高量子点材料的质量和优化量子点激光器材料的结构，研制出高质量的应变自组装InAlAs/AlGaAs/GaAs量子点材料和低温连续激射的红光量子激光器。器件性能达到国际同类研究的最好水平

The analysis of the genotyping of plasmid-mediated AmpCβ-lactamases produced by clinical strains of Escherichia coli and Klebsiella pneumoniae

目的针对该院临床分离大肠埃希菌和肺炎克雷伯菌质粒型AMPC酶基因型进行研究分析。方法收集2011年7月至2012年8月对头孢西丁不敏感无重复临床分离大肠埃希菌和肺炎克雷伯菌共176株,采用聚合酶链反应(PCr)法和扩增全基因序列分析大肠埃希菌和肺炎克雷伯菌产AMPC酶基因型。结果 PCr结果显示,AMPC酶基因(AMPC基因)阳性率为18.2%主要以dHA型为主,阳性率为59.4%,CIT型为37.5%,EbC为3.1%;其中,大肠埃希菌AMPC基因阳性率为11.4%,以CIT型为主,阳性率为77.8%,dHA型和EbC型阳性率均为11.1%;肺炎克雷伯菌AMPC基因阳性率为23.7%,以dHA型为主,阳性率为78.3%,CIT型为21.7%。基因序列结果显示,dHA型有18株为dHA-1基因型和1株摩根摩根菌AMPC基因型,一致率97.0%,CIT型有10株为CMy-2基因型,1株CMy-42基因型和1株CMy-4基因型;EbC型为阴沟肠杆菌AMPC基因型,一致率为99.0%。将32株基因序列提交gEnbAnk,均被接受,其登录号为kJ127248~kJ127279。结论该院临床分离大肠埃希菌AMPC基因主要以CMy-2型为主,而肺炎克雷伯菌主要以dHA-1型为主。Objective To investigate the genotype and epidemiology of plasmid-mediated AmpCβ-lactamases produced by the clinical strains of Escherichia coli and Klebsiella pneumoniae.Methods A total of 176 clinical nonrepetitive cefoxitin non-sensitivity isolates of Escherichia coli and Klebsiella pneumoniae was collected from July 2011 to August 2012.Polymerase chain reaction(PCR)for AmpC enzyme gene amplification and DNA sequencing were carried out for genotype of AmpC beta-lactamases.Results The results of PCR showed that the positive rate of ampCof the 176 strains of Escherichia coli and Klebsiella pneumoniae AmpC was 18.2%,mainly DHA type,counting for 59.4%,CIT counting for 37.5%,EBC counting for 3.1%.The positive rate of ampC of Escherichia coli was 11.4%,mainly CIT type,counting for 77.8%,the positive rates of DHA type and EBC type both were11.1%.The positive rate of ampCof Klebsiella pneumoniae were 23.7%,mainly DHA type,counting for 78.3%,CIT type counting for 21.7%.The results of DNA sequencing showed that there were 18 strains DHA-1type and 1strain ampCgene type of Morganella morganii in DHA type strains,the concordance rate was 97.0%,10 CIT type strains was CMY-2type,1strain was CMY-42,one strain was CMY-4type,EBC type was ampCgene type of Enterobacter cloacae,the concordance rate was 99.0%.A total of 32 strains of gene sequencing were registered as KJ127248-KJ127279 in GenBank.Conclusion The main genotypes of plasmid-mediated ampCenzyme produced by Escherichia coli and Klebsiella pneumoniae were CMY-2and DHA-1respectively.福建省自然科学基金项目(2013D002); 福建省卫生厅青年基金项目(2010-2-90

Clinical value of fluorescent PCR-probe melting analysis in detection of plasmid-mediated AmpC β-lactamase genes

目的探讨多重荧光PCR-探针熔解分析法应用于临床分离大肠埃希菌和肺炎克雷伯菌中ampC耐药基因的检测方法,并评价该方法的临床应用价值。方法收集医院2009年7月-2010年6月的临床分离菌株,首先采用头孢西丁纸片法进行耐药表型筛选,再同时应用传统PCR技术与荧光PCR-探针熔解曲线法对临床分离菌株进行检测,并对ampC耐药基因扩增产物进行DNA测序。结果经头孢西丁纸片法筛选出176株对头孢西丁不敏感临床分离菌株,其中97株为肺炎克雷伯菌、79株为大肠埃希菌;在176株临床分离株中,荧光PCR-探针熔解分析法检测出36株ampC耐药基因阳性菌株,包括18株DHA型、12株CIT型和5株EBC型,还有1株肺炎克雷伯菌同时含有DHA型和EBC型;而传统PCR技术检出32株ampC耐药基因阳性,两个检测结果的符合率为97.7%;DNA测序后经BLAST比对,荧光PCR-探针熔解分析法检测ampC基因型与所检测目的基因型一致。结论荧光PCR-探针熔解分析法能高效检测出质粒介导ampC耐药基因,其方法简便、敏感性高、特异性强,具有良好的临床应用价值。OBJECTIVE To explore the clinical value of multiplex real-time PCR-probe melting analysis in detection of ampC drug resistance gene in clinical isolates of Escherichia coli and Klebsiella pneumoniae.METHODS The clinical isolates were collected from Jul 2009 to Jun 2010;the drug resistance phenotypes were screened by using cefoxitin disk agar diffusion method,the clinical isolates were detected by means of the traditional PCR technique and fluorescent PCR-probe melting curve method,and the DNA sequencing was performed for the amplified products of the ampC drug resistance gene.RESULTS Totally 176 clinical isolates that were not sensitive to cefoxitin were screened out by using cefoxitin disk method,including 97 K.pneumoniaeisolates and 79 E.coli isolates.Of the 176 clinical isolates,36 were detected positive for the ampC drug resistance gene by using the fluorescent PCR-probe melting analysis method,including 18 strains positive for DHA,12 strains positive for CIT,5strains positive for EBC,and 1strain positive for both DHAand EBC;while 32 strains were detected positive for the ampC drug resistance gene by the traditional PCR technique,the coincidence rate of the two detection results was97.7%;the DNA sequencing analysis showed that by contrast to BLAST,the ampCdrug resistance gene detected by the fluorescent PCR-probe melting analysis method was consistent with the target genotype.CONCLUSIONThe fluorescent PCR-probe melting analysis method can effectively detect the plasmid-mediated ampCdrug resistance gene,it is simple,highly sensitive and specific and worthy to be promoted in hospitals.国家自然科学基金资助项目(81000762);; 福建省自然科学基金资助项目(2013D002);; 福建省卫生厅青年基金资助项目(2010-2-90);; 厦门科技局基金资助项目(3502z20089003