香豆素-半花菁-苯硼酸对唾液酸的识别与光学传感

以共轭连接的香豆素和半花菁为信号报告基团,以苯硼酸为识别基团,设计合成了一种对唾液酸具有选择性识别的比色型荧光传感分子,命名为CHB,并采用吸收和荧光光谱方法研究其对唾液酸(N-乙酰神经氨酸)及其他糖类分子(如葡萄糖、蔗糖和麦芽三糖等)的响应.结果表明,CHB仅对N-乙酰神经氨酸表现出显著的吸收和荧光光谱响应,并通过高分辨质谱研究证实二者以1∶1计量比通过共价作用结合形成复合物.N-乙酰神经氨酸与苯硼酸的共价作用和静电作用共同影响了香豆素-半花菁共轭体系的分子内电荷转移,从而引起显著的溶液颜色变化和高选择性的光谱响应,因此CHB可用于N-乙酰神经氨酸的可视化检测.国家自然科学基金(21435003,91427304,21521004,J1310024

Study on Direct Reduction in Fluidized Bed and Magnetic Separation of Fine Distributed Hematite

随着我国钢铁工业规模的不断扩大，我国己成为世界上最大的铁矿石进口国，进口铁矿石的数量己占我国铁矿石需求总量的一半以上。2005年之后，进口铁矿石的价格大幅度提高，对我国钢铁工业的发展产生巨大的压力，开发利用我国自有的铁矿石迫在眉睫。而我国自有铁矿石品位低，嵌布粒度细，目前的选矿技术很难对这部分铁矿石进行利用。 磁化焙烧是处理难选赤铁矿比较有效的方法之一，但是，对于细嵌布粒度的铁矿石，磁化焙烧－磁选已不能将其分离。因为只有当矿石颗粒被磨到很细时才能达到单体解离，但此时铁矿颗粒和脉石的比磁化系数差很小，在磁场中，铁矿颗粒所受的磁场力小于机械力，所以铁矿颗粒随水流排入尾矿，降低了选矿指标。 直接还原是将铁矿石直接还原为金属铁。金属铁与脉石的延展性差异大，对还原矿进行磨矿，金属铁被磨为具有较大比磁化系数的铁片，脉石被磨为粒径很小的颗粒而脱离铁片，两者的比磁化系数差异显著。磁选时，金属铁片所受的磁场力大于它所受的机械力，被留在磁场中，而脉石不受磁场力作用，随水流排入尾矿。 本文选取云南省召夸镇的铁矿石进行实验，该矿属低品位赤铁矿，且嵌布粒度细、分布不均。实验中采用流化床作为磁化焙烧和直接还原装置，分别以CO-N2和H2-N2作为还原气，考察磁化焙烧－磁选和直接还原－磁选的影响因素。结果表明：直接还原－磁选所得精矿指标要明显优于磁化焙烧－磁选所得指标，精矿品位由51.69%提高到了73.79%，而回收率基本不变。以H2-N2做还原气时还原矿金属化率为86.52%，比由CO-N2作还原气时的64.08%提高了20%。将所选矿石在H2-N2气氛中的流化床中进行直接还原所得还原矿进行二次磨矿，然后在磁选管中磁选，矿石品位由26.78%提高到了73.79%，铁回收率也可达到76.10%，比按传统的磁化焙烧－磁选方法所得结果有较大的提高。 从实验中可以得出，流化床中直接还原效率高。磁选前的磨矿不仅增大了单体解离度，也增大了脉石和矿石比磁化系数的差，有利于后续的磁选。直接还原－磁选工艺对于细粒级难选铁矿的选矿有较明显的效果

单片微型计算机在单模光纤定点衰耗测试仪中的应用

本文从硬件和软件两方面介绍了单片微型计算机在单模光纤定点衰耗测试仪中的应用。并对光纤参数测试仪表的微型化和智能化作了初步探讨

云南召夸赤铁矿流化床直接还原—磁选试验

召夸赤铁矿原矿品位低,嵌布粒度细。采用流化床作为直接还原装置,以H2与N2的体积比为1∶4的混合气体作为还原气,对召夸赤铁矿进行直接还原—磁选试验。结果表明:在700℃下将磨矿细度为-150目占92%的原矿直接还原70min,可得金属化率为85.89%的还原矿。将还原矿在球磨机中细磨至平均粒径为3.98μm,在场强为63.66kA/m的磁选管中磁选,可得品位为73.04%,回收率为77.28%铁精矿

Dual Flrorescence of Anilino Substituted Benzanilides in Non-polar Solvent Cyclohexane——Direct Spectroscopic Evidence for the Excited-state Charge Transfer

The f luo rescence spect ra in cyclohexane of a series of benzan ilides (BA s) with different p a ra2sub st ituen t s at the an ilino mo iety revealed that BA s em it ted dual f luo rescence, w ith one no rm al w eak em ission at ca. 330 nm and an abno rm ally long2w avelength em ission ranging f rom 460 to 550 nm. The long2w avelength em ission w as found st rongly dependen t of the elect ron donat ing capacity of the sub st ituen t at the an ilino mo iety and a st ronger elect ron donat ing sub st ituen t led to a red2sh if t in the em ission. The energies of the long2w avelength em ission of the BA s w ere co rrelated to the ox ida2t ion po ten t ials of the dono rs, EDöD + , and a n ice linear co rrelat ion w as found w ith a slope of + 0166, w h ich clearly po in ted to the charge t ran sfer character of the em issive state fo r the long2w avelength e2 m ission and the h igh decoup ling ex ten t of the charges in the CT state. Th is is the f irst repo rt that show s the direct evidence fo r the CT natu re. The resu lt sm igh t be of sign if icance in understanding the photophysics of pep t ide and p ro tein and in designing novel fluorescent chemosensors.国家自然科学基金 (批准号 :2 9975 0 2 32 0 175 0 2 0 );教育部优秀青年教师奖励计划 (2 0 0 1年 );德国大众基金 (批准号 :I/770 72 )资助

Bacterial diseases of Haliotis diversicilor supertexta in Dongshan,Fujian

本文分离纯化了 1 999年春东山县患病九孔鲍的 2株主要病原菌 ,进行了回归感染、药敏试验、病变组织的超薄切片观察 .结果表明此次暴发性流行鲍病的致病菌主要是溶藻弧菌和副溶血弧菌 .在所进行的 48种药物的药敏试验中 ,2株菌仅对氯霉素和复方新诺明等 8种药物共同敏感 ,药物联合抗菌试验还表明复方新诺明与磺胺甲基异恶唑等有协同作用 ,氯霉素与复方新诺明等有加成作用 .A serious epidemic of Haliotis diversicilor supertexta was broken out in Donshan of Fujian during spring in 1999. Two strains isolated from diseased H. diversicilor supertexta were used to do artificial infection experiments with the healthy abalones and confirmed to be pathogenic. According to their morphological and biochemical characteristics, two strains of the isolates were identified as Vibrio alginolyticus and V. parahaemolyticus . Among the 48 detective drugs, few drugs were effective to both strains and only four drugs could promote each other. Vibrioes resulted in this epidemic of the abalones. Besides this, adverse environment was also an important factor. Good cultural environments, strongly immune abilities of the abalones and suitable medicines can prevent and cure diseases of abolones

适用于低速UWB的多径接收算法改进

叉指数优化选择是传统MMSE-Rake（最小均方差Rake接收机）算法的重要组成部分,广泛应用于UWB（超带宽）Rake接收机中。为了提高在低速传输条件下UWB系统对多径信号接收的快速识别能力,提出了一种基于优化抽头系数的MBER（最小误比特率）改进算法,该算法可在不降低多径分辨率的前提下,通过简化Rake叉指数实现快速定位。仿真结果表明,在低速CM3（NLOS信道）和CM4（特殊情况下NLOS信道）多径模拟信道传输条件下,改进后的算法在性能上优于传统MMSERake算法,不仅降低了Rake接收机的复杂度,还提高了信号接收的实时性

Spring Explosive Epidemic Disease of Abalone in Dongshan District

对 1999 年春季福建省东山县九孔鲍的暴发性流行病进行调研和检测，对细菌性病原进行分离纯化、回归感染确定致病原、对致病菌进行形态（包括电镜和光镜观察）和生理生化鉴定；对病毒性病原进行电镜负染和超薄切片观察、以及病理变化分析.结果表明引起此次东山养殖九孔鲍暴发性流行病的主要致病原是 3 种球状病毒（50 nM 、110 nM 、150 nM ）、溶藻弧菌和副溶血弧菌.还探讨了这些病菌和病毒的致病性，并以 49 种药敏纸试验寻求防治的有效药物.An explosive epidemic disease of abalone Haliotis diversicolor supertexta was broken out in Dongshan district during the period from February to May in 1999.Both bacteria and virus isolated from the diseased abalone were injected into healthy abalone to check the pathogens of this serious disease.Two vibrios ( Vibrio alginolyticus、V.paralaemolyticus ) and three forms of spheroid virus (50 nm、110 nm、150 nm) were identified by physiological、biochemical and electron microscopical methods.49 antibiotics were also tested to see the sensitivities to these pathogens so as to seek the prevention and cure of this serious disease

Preliminary study on chemical cues in the conspecific aggregation of Styela canopus

研究了不同浓度的冠瘤海鞘成体被囊萃取物和幼体萃取物及不同的幼体密度对冠瘤海鞘(Styelacanopus)幼体附着和变态的影响.结果表明,冠瘤海鞘被囊萃取物和幼体萃取物在5%和10%的浓度下均促进冠瘤海鞘幼体的附着和变态,但被囊萃取物的诱导效果明显强于幼体萃取物;幼体密度高于25只/cm3时,促进冠瘤海鞘幼体的附着和变态;幼体密度低于10只/cm3时,幼体的附着和变态不受密度影响.这些结果提示海区中冠瘤海鞘的同种群聚可能与其被囊或幼体内含诱导幼体附着和变态的化学因子有关.The effects of conspecific adult tunic extracts and larval tissue extracts in different concentrations and the effects of different larval density on settlement and metamorphosis in the Styela canopus larvae were investigated.Experiments show that conspecific adult tunic extracts and larval tissue extracts both were able to induce Styela canopus larvae to settle and metamorphose in the concentrations of 5% and 10%.It is found that Styela canopus larvae are induced to settle and metamorphose at densities of more than 25 larvae per millilitre and the settlement and metamorphosis are not influenced at densities of lower than 10 larvae per millilitre.All results suggest that the ability of chemical factors in adult tunic extracts or larval tissue extracts to induce settlement and metamorphosis may explain the conspecific aggregation of Styela canopus in nature.国家自然科学基金资助项目(49976034)

Influences of marine adhesive bacteria on settlement and metamorphosis of Styela conopus Savigny larvae

从冠瘤海鞘、皱瘤海鞘及乳突皮海鞘的被囊表面及其附着基和附近的海水中分离到290株细菌,根据其形态和生理生化特征,将其鉴定至弧菌属、肠杆菌属和无色杆菌属等10个属.分析海鞘被囊表面及其附着基和海水中的细菌组成,并从各属中挑出的29株细菌中分离筛选出附着能力较强的9株.将这9株细菌分别制成不同的单种细菌黏膜及9种细菌混合黏膜,检测细菌黏膜对冠瘤海鞘幼体附着和变态的影响.结果表明不同细菌黏膜对冠瘤海鞘幼体的附着和变态起不同的作用.无色杆菌属H-13显著地促进海鞘的附着和变态;弧菌属的H-4对海鞘的附着和变态均起抑制作用;弧菌属H-9明显地促进附着却强烈抑制海鞘幼体变态;其余细菌仅仅对海鞘幼体附着或变态的一个过程起作用或是对两者都没有明显效果.One hundred and eighty bacteria isolated from surface of tunics of Styela conopus Savigny, Styela plicata Lesueur, Molgula manhattensis Delay and their adhesive substrates, as well as 110 ones from nearby water, were investigated . On the basis of their morphological, biochemical and physiological characteristics, these bacteria belong to 10 genera and over 60% bacteria are Gram-negative. In bacteria from surfaces of sea squirt's tunics and their adhesive substrates, dominant colonies are Pseudomonas, while Aeromonas are primary in bacteria from water. Nine strains were screened out from 180 bacteria for their great ability of attachment. Then these 9 bacteria were cultured to form different bacterial films respectively, together with a mixed artificial film made of all 9 strains so that influences of these artificial films on settlement and metamorphosis of Styela conopus Savigny larvae were studied. It is revealed that different types of artificial films have dissimilar effects on settlement and metamorphosis of Styela conopus Savigny larvae. On one hand, 5 kinds of bacterial films, H6, H9, H13, H15 and the mixed film remarkably stimulate larva attachment, while H4 strongly inhibited and others are without evident influences. On the other hand, in 10 bacterial films, H4, H7, and H9 obviously restrain metamorphosis of lava of Styela conopus Savigny, but more larva are metamorphic when they grew on H13 and the mixed bacterial film. It illustrated that settlement and metamorphosis of Styela conopus Savigny larva were two correspondingly independent processes during these two courses, different effects were shown when larva grew on the same bacterial film.国家自然科学基金资助项目(49976034)