Primary Investigation of Optical Molecular Chemosensors for biological thiols and HClO

摘要 分子传感器的重要特征就是能够将物质的化学组成信息转换为可测量的光电信号，因而具有灵敏度高、操作简便、易于实现快速检测的优点。在生命科学的高端研究领域具有无可代替的作用，分子传感器不断受到重视和发展。本文在前人工作的基础上根据一些特异性化学反应设计合成了若干光学分子传感器。本论文共分四章，分别包括以下内容： 第一章，绪论，首先简要介绍了光学分子传感器的基本概念；其次重点介绍了巯基氨基酸、次氯酸的光学分子传感器的研究现状和进展；最后，对这些相关研究进行分析总结，结合本实验室的工作基础和现有条件，提出本论文的研究设想。 第二章，研究了哌嗪取代七次甲基花菁作为巯基氨基酸光学分子传感...Abstract Optical molecular chemosensors (OMCSs) obtain one of the most important point is that it can transform the information of chemical composition in substance to the analytical useful signals. So it has the advantages of high sensitivity, easily operate and fast detection. OMCSs are highly applicable to advanced researches in life sciences. Therefore, OMCSs are attracting much current resea...学位：理学硕士院系专业：化学化工学院_分析化学学号：2052010115153

Study of Multifunctional Corrosion Inhibition of Zn-Al Layered Double Hydroxides and Its Derivatives for Steel in Concrete

钢筋腐蚀是引起钢筋混凝土结构过早失效的主要原因，是当今腐蚀与防护领域研究的热点和难点问题之一。开展钢筋混凝土的腐蚀防护研究具有重要的理论和实际意义。混凝土的碳化和氯离子的侵蚀是导致混凝土中钢筋腐蚀的主要因素。添加钢筋缓蚀剂是一种经济而有效的腐蚀防护措施。针对混凝土中钢筋腐蚀的特点，本论文工作发展了一种层状双金属化合物（及衍生物），多功能钢筋缓蚀剂，利用各种方法对缓蚀剂性能和功能进行评价，并初步研究了缓蚀剂对钢筋的缓蚀机理。主要取得以下研究结果： 1.在碳酸盐溶液环境下制备了Zn-AlCLDHs，发现NaCl溶液经Zn-AlCLDHs浸泡处理3h后，钢筋的腐蚀电流密度明显降低。利用Cl-探针和...The corrosion of steel is one of the main reasons causing the premature failure of reinforced concrete construction, and it has become a research focus in the field of corrosion and protection in the world. It is significant and urgent to further study the corrosion and protection of steel in concrete from theoretical and practical aspects. The carbonation of concrete and ingress of Cl- are known ...学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：2052009115142

Study on the legal issues related to Seaworthiness

船舶适航历来是海上货物运输承运人的一项基本首要义务，然而由于国际公约和各国法律在规定上的含糊不明，长久以来这一问题存在着不少误区和争议。基于此种原因，本文试图通过系统分析理清船舶适航性方面存在的一些疑问，希望能对我国的海运实践起到一定的借鉴作用。 本文分为引言、正文、结语三个部分。正文内容分为四章： 第一章“船舶适航性的历史演进”，以时间为序，通过对各时期代表性海商海事法的相关条文进行分析，展示了适航性的萌芽、进步和变革的历程。 第二章“船舶适航性的涵义及时间界定”，从客观层面（船体适航、船员适航、载货处适航）和主观层面（承运人是否尽谨慎处理义务）分析了适航的涵义。通过对开航前和开航时的...As a focus of international maritime law society and transporting circle， the seaworthiness of vessel has always been a basic and paramout obligation of engage in carriage of goods by sea. However， since the ambiguity of the well as the difference in practice， there have been lots of controversy and misunderstandings regarding to this issue for a long time. For this reason， this article starts fro...学位：法律硕士院系专业：法学院法律系_法律硕士(JM)学号：1292006115065

浅谈法律解释中形式合理与实质合理

在对疑难案件的裁判过程中,对不同判决方案预测其社会效果并权衡其利弊得失是隐含于法律解释过程中的思维过程,其中所隐含的法律解释逻辑及深层理论基石是形式合理性与实质合理性之间的博弈。而能动的运用社会学解释方法能够巧妙地解决法律解释中形式合理与实质合理这一矛盾之间的张力

重组人血管内皮生长抑制因子对人肺腺癌裸鼠移植瘤的放射效应影响

目的:探讨重组人血管内皮抑制因子(recombinant human vascular endothelial growth inhiloitor-192,rhVEGI-192)对人肺腺癌裸鼠移植瘤的放射增敏作用。方法:采用原核表达rhVEGI-192,获得目的蛋白。通过肿瘤倍增时间,计算药物的增敏系数。通过建立人肺腺癌裸鼠移植瘤模型,荷瘤裸鼠随机分为4组:对照组、10Gy、rhVEGI-192、rhVEGI-192+10Gy。采用6MV-X线进行照射,照射剂量为10Gy。获得移植瘤标本,利用免疫印迹法检测移植瘤中VEGF(vascular endothelial growth factor)的表达变化。结果:SDS电泳结果显示,目的蛋白位于22k D左右。10Gy照射时,重组人血管内皮抑制因子的EF(enhancement factor)值为1.5。和空白对照组相比,rhVEGI-192组和10Gy组移植瘤的生长受到抑制(P<0.001),rhVEGI-192+10Gy组移植瘤生长显著抑制(P<0.001),rhVEGI-192+10Gy组移植瘤较10Gy组有明显生长抑制。和空白组相比,rhVEGI-192组VEGF表达减少,而10Gy组VEGF表达变化不明显,rhVEGI-192+10Gy组VEGF表达明显减少。rhVEGI-192+10Gy和rhVEGI-192组相比,VEGF表达减少。结论:rhVEGI-192联合照射能够减少VEGF的表达。这可能是rhVEGI-192的增敏机制之一。中国人民解放军南京军区医学科技创新项目(编号:No.12MA061

A Shadow Detection Algorithm Research Based on HSV Space Features and Texture Features

为了提高检测和跟踪算法的准确性,提出了一种基于阴影在HSV空间下的特点和纹理特征的阴影检测与去除算法。该算法针对阴影区域覆盖的地面和未被阴影区域覆盖的地面纹理特征基本不变的特点,先检查序列图像中的运动区域,然后在运动区域内,根据阴影在HSV空间下的特点和纹理特征对阴影进行检测与去除,为运动目标的后续处理排除阴影干扰。实验结果表明,该算法能够很好地抑制噪声,有效检测、去除图片中运动目标的阴影。In order to improve the accuracy,this paper proposes a shadow detection and removal algorithm based on the features of HSV space and texture.Either the area is covered by the shadow or not,it always has the same texture feature.Based on this point,the algorithm firstly detect the moving area in series images,and then in moving area,based on the different characteristics of shadow when in HSV space and its texture feature,it is detected from the moving target and it is removed disturbance for the following processing.Experimental results show that the proposed algorithm can detect the noise and shadow and remove them effectively

Inhibitory effect of anti-human NRP-1 monoclonal antibody on hepatocellular carcinoma cell line HepG2 and its mechanism in vitro

目的研究抗人神经纤毛蛋白-1(Neuropilin1,NRP-1)单克隆抗体对肝癌Hep G2细胞的生长抑制作用及其机制。方法小鼠腹水法制备抗NRP-1单克隆抗体(NRP-1 m Ab)并用r Protein A亲和柱纯化抗体,间接ELISA检测抗体的滴度水平。Western blot检测NRP-1 m Ab对Hep G2细胞的特异性,细胞免疫荧光和流式细胞术检测NRP-1蛋白在肝癌细胞株Hep G2上的表达,MTT法检测NRP-1 m Ab对Hep G2的生长抑制作用,Western blot检测ERK1/2、P-ERK1/2、Akt、P-Akt蛋白的表达水平。结果 SDS-PAGE和间接ELISA检测纯化的NRP-1 m Ab纯度为95%以上,效价为1×10~(-6);Western blot检测结果显示NRP-1 m Ab可与Hep G2细胞膜上的NRP-1蛋白特异性结合。细胞免疫荧光染色结果显示NRP-1定位于Hep G2细胞膜,流式细胞术结果显示NRP-1蛋白在Hep G2细胞上表达水平较高;MTT法检测结果显示NRP-1 m Ab对Hep G2细胞有生长抑制作用。Western blot检测到在不同浓度NRP-1 m Ab作用下,Hep G2细胞裂解液P-ERK1/2、P-Akt蛋白的条带信号逐渐减弱。结论纯化的NRP-1m Ab能抑制Hep G2细胞的生长,其抑制作用是通过EGF和HGF信号通路实现的。The aim of the experimental is to investigate the inhibitory effect of anti-human nerve cilia protein1(Neuropilin-1,NRP-1) monoclonal antibody(NRP-1 mAb) on hepatocellular carcinoma cell line HepG2 and its mechanism in vitro.Anti-human NRP-1 monoclonal antibody(NRP-1 mAb) was prepared from mouse ascites and purified by rProteinA affinity column assay.The titer of antibody was determined using indirect ELISA assay;the characteristic of NRP-1 mAb binding to NRP-1 was determined using Western blotting;the expression of NRP-1protein in hepatocellular carcinoma cell line HepG2 was determined using immunofluorescence assay and flow cytometry assay.Growth inhibition of HepG2 cells treated with different concentrations of NRP-1 mAb was determined using MTT assay,while Western blotting was used to detect the expression levels of ERK1/2,P-ERK1/2,Akt and P-Akt proteins.The results of SDS-PAGE and indirect ELISA showed that the purity of purified NRP-1mAb was more than 95%and the titer was 1×10~(-6).Western blotting analysis suggested that NRP-1 mAb could bind specifically to NRP-1 on HepG2 cell;immunofluorescence staining showed that NRP-1 was located in the membrane of HepG2 cells.Flow cytometry analysis showed that the expression level of NRP-1 on HepG2 cell was relatively high.Western blotting analysis suggested that P-ERK1/2 and P-Akt expression levels were down-regulated after having incubated HepG2 cells with different concentrations of NRP-1 mAb.In conclusion,NRP-1 mAb could inhibit the growth of HepG2 cells(P<0.05),and its inhibitory effect is achieved by reducing the P-ERK1/2 and P-Akt expression.南京军区医学科技创新项目(12MA061,15MS104

Distribution Characteristics of Polycyclic Aromatic Hydrocarbons in Seawater from the South Yellow Sea

2007年9月进行了2007南黄海海洋环境调查,并采集各个调查站位的表层、中层、底层海水及表层沉积物。利用液-液萃取法对采集到的样品进行前处理,并用气相色谱-质谱法检测海水中的多环芳烃。结果显示,监测站位中表层海水中总多环芳烃的含量范围是15.76--233.39ng/l,南黄海中部海水中多环芳烃的组成以2环的萘为优势组分;各种多环芳烃的含量与国际生物学组织或国家制定的评价水生生物暴露于水体的安全食用标准相比虽未超标,但个别组分如荧蒽的含量已达到生态毒理评价标准。Marine surface sediments and sea water samples from the surface, middle and bottom layers were collected from the South Yellow Sea of China.The samples were pretreated by liquid-liquid extraction and the 16 polycyclic aromatic hydrocarbons (PAHs) in the samples were determined by gas chromatography-mass spectrometry (GC-MS).The results show that the range of total PAHs is 15.76～233.39 ng/L in surface water and PAHs in the water samples from central South Yellow Sea are dominated by naphthalene (2 rings).Content of PAHs compounds don′t exceed the safety guidelines proposed by International Biology Organization or governments, but the individual compound, such as fluoranthene content has reached eco-toxicological evaluation criteria.国家海洋局节能减排专项资助;中国海监技术支撑项目资助;海洋公益性项目资助(200705011

粗糙铂电极上甲酸吸附氧化的电化学原位表面增强拉曼光谱研究

采用循环伏安法和电化学原位表面增强拉曼光谱 (SERS)技术研究甲酸的解离吸附与氧化行为 .首次报道了甲酸吸附、解离和氧化的电化学原位SERS谱 ,发现甲酸在粗糙铂电极上能自发解离吸附 ;首次成功地获得了粗糙铂电极上甲酸吸附解离的强吸附中间体CO和活性中间体COOH的表面增强拉曼光谱 ,同时首次检测到甲酸氧化最终产物CO2 的拉曼光谱信号 ,从分子水平证实甲酸解离吸附反应的双途径机理

Concentration and distribution of polycyclic aromatic hydrocarbons in surface sediments collected in the Southern Yellow Sea

采用气相色谱与质谱联用(gC/MS)技术,在一个航次内对南黄海表层沉积物中16种优先监控的PAHS的污染状况进行了调查,采用菲/蒽、荧蒽/芘、荧蒽/(荧蒽+芘)、吲哚芘/(吲哚芘+苯并(g,H,I)苝)等特定比值对PAHS来源进行了分析.结果表明,南黄海表层沉积物中检出PAHS的总含量为90.4~732.65ng·g-1,各站点均以4~6环为主;与其它站位相比,倾废区的H01站位受到PAHS污染较为明显,无论是16种PAHS总量还是高分子量组分最高值都出现在该站点,虽然该海区沉积物中PAHS的含量没有超出生物影响低值,但苯并(b)荧蒽、吲哚芘和苯并(g,H,I)芘等一些没有最低安全标准的PAHS也有不同程度的检出,对海洋生物具有潜在的毒副作用.PAHS可能来源于原油、生物和煤燃烧造成的污染.The concentrations of sixteen representative Polycyclic Aromatic Hydrocarbons(PAHs) in surface sediment samples collected from the Southern Yellow Sea were determined by GC-MS.The distribution and potential sources of PAH contamination in the region were investigated.The types of PAHs found in the surface sediments were mainly 4～6 ring PAHs,and they are unevenly distributed among the different sampling sites covering an area of 139 km2.The area is known for continuous waste dumping activities in recent years,and indeed sites adjacent to such activities were found to have high levels of PAHs.Some high molecular weight PAH compounds such as benzo$$b$$fluoranthene,dibenzo$$a,h$$anthracene and benzo$$g,h,i$$perylene were detected in some samples.The health implication of these contaminants needs careful assessment since toxicity guidelines are not available for these species.The potential sources of PAHs are discussed using various source-specific PAH indexes such as Phenathrene/Anthracene、Fluoranthene/Pyrene、Fluoranthene/(Fluoranthene+Pyrene) and Indeno [1,2,3-cd] pyrene/(Indeno[1,2,3-cd] pyrene+Benzo [g,h,i] perylene).The composition of the observed PAHs reflects that they originate from both petroleum utilization and incomplete combustion.海洋公益性行业科研专项(No.200705011);中国海监技术支撑体系项目;2008年海洋环境保护及节能减排专项---