Experimental study on effect of Changrun Formula in regulating expression of AQP3 and AQP9 in colon mucosa of functional constipation rats

目的:观察肠润方对功能性便秘模型大鼠结肠黏膜AQP3、AQP9表达的影响,探究其调控AQP3、AQP9表达的分子机制。方法:60只SD大鼠随机分为空白组、模型组、阳性对照组(麻仁丸组)、肠润方组、肠润方联合P38MAPK抑制剂SB203580组。用复方地芬诺酯制造大鼠便秘模型,采用免疫组织化学及实时荧光定量PCR(q RT-PCR)检测大鼠便秘模型近端及远端结肠黏膜AQP3、AQP9的表达;Western Blot检测信号传导通路相关分子P38MAPK及P-P38MAPK的表达。结果:造模成功后,模型组近端结肠黏膜AQP3表达较空白组明显上升,而远端结肠黏膜AQP9表达较空白组明显下降(t值分别为3.148和7.069,P值均0.05)。肠润方联合P38MAPK抑制剂SB203580治疗后,近端结肠黏膜AQP3 m RNA水平显著上升(t=5.922,P0.05). The level of AQP3 m RNA in proximal colonic mucosa was significantly increased(t=5.922, P<0.01), and AQP9 m RNA was significantly decreased in distal colonic mucosa(t=4.038, P<0.01); The relative expression of P-P38/P38 protein was significantly decreased(t=19.419, P<0.01). Conclusion: The therapeutic action ofChangrun Formula on functional constipation rat models might through inhibiting the expression of AQP3 in proximal colon, and accelerating on expression of AQP9 in distal colon, and the regulating mechanism might relate with the inhibition on P38 MAPK and AKT phosphorylation.福建省自然科学基金项目(No.2012D043)~

湛江湾和平水母属2新种（软水母亚纲，锥螅水母目，和平水母科）

采自广东湛江湾海域软水母亚纲(Leptomedusae Claus,1877)、锥螅水母目(Conica Broch,1916)、和平水母科(Eirenidae Haeckel, 1879)、和平水母属(Eirene Eschscholtz,1829) 2新种,即大腺和平水母新种(E. macrogonia Huang, Sun et Liu,sp. nov.)和湛江和平水母新种(E. zhanjiangensis Huang, Zhang et Zao, sp.nov.)。其鉴别特征如下:(1)大腺和平水母,新种;伞扁于半球形,胶质中等厚;胃柄基部塔状,胃柄较长,伸出伞腔口外;垂管长度短于口唇,4个发达口唇边缘呈齿状皱褶;生殖腺发达波状弯曲,着生于辐管下伞部,从胃柄基部延伸到伞缘外;19～24条缘触手,5～8个缘疣,触手基部呈球状,无排泄乳突;每2条触手间或触手和缘疣间有1个平衡囊,每个平衡囊有1～2个平衡石;4条辐管,1条环管。(2)湛江和平水母,新种;伞半球形,胶质厚;胃柄基部呈塔形,末端变窄;垂管较长,约等于口唇长度,口唇边缘有齿状皱褶;生殖腺带状,从胃柄基部延伸至近伞缘;缘触手多,70～125条,触手基球有排泄乳突;平衡囊数目约为触手数的1/2;4条辐管,1条环管。国家海洋局海洋公益性科研专项（No.201505027

偶-偶Er同位素核的γ自由度效应

根据 Davydov 和 Filippov 的非轴对称转子模型,从实验的 E(2_2~+)/E(2_1~+)以及 E(2~+_1)值,提取了偶-偶 Er 同位素核的四极形变β与非轴对称γ参数.结果表明,Davydov 和 Filippov 关于非轴对称转子的理论,与过渡区核的低激发谱的实验结果相符合,表明在~(157)Tm 中非轴对称性是重要的.Based on the non-axial rotor model given by davydov and Filippov,the deformation parameter β and non-axial parameter γ of even-even Er isotopes are determined from the energy of the first 2~+ level and the energy ratio of the second 2~+ level and the first one.The present work indicates that this model is consistent with the experimental results in the transitional region.It also shows that the non-axial effects on ~(157)Tm nuclide are of importance

关于奇ATm核1／2~＋［411］转动带的系统分析

壳模型计算表明，１／２＋［４１１］出现于１５７Ｔｍ核的基态可能是由非轴对称形变造成的．但是新近从１５７Ｙｂ衰变纲图给出的１５７Ｔｍ低激发谱中，指认了一个建立在１／２＋［４１１］带头上的基态转动带，并认为该带的性质是轴对称的，提取了该带的惯性参数与脱耦合参数．通过对奇ＡＴｍ核１／２＋［４１１］转动带的系统分析，强调了在１５７Ｔｍ核中非轴对称γ自由度效应的重要性。The nuclear spin of grotmd state in 157Tm is I=1/2 This spin may be explainedby a non-axisymmetric quadruploe deformationγof 157Tm The recent experiment ofEC/β+ decay of 157 Yb does not support this explanation.However,thenonaxisymmetric property of 1/2+[411] band in 157Tm is confirmed by our systematic analysis of 1/2+[411] rotational bands in odd-A Tm nuclides

一种具有石墨烯结构的非晶碳膜及其制备方法

本发明提供了一种具有新型特殊结构的非晶碳膜，该非晶碳膜分为上下两层，下层是非晶碳膜，上层位于下层表面、是厚度约为0.5～10nm的多层石墨烯结构薄膜，这为制备石墨烯提供了一种全新的思路。采用磁控溅射沉积法，包括高功率脉冲磁控溅射沉积或普通直流磁控溅射沉积，以高纯石墨为靶材，通过调节衬底负偏压、脉冲磁控溅射电压等参数，在惰性气体保护氛围中，在衬底上溅射沉积0.5～180min而得到该表面具有多层石墨烯结构的非晶碳膜。与现有的石墨烯制备技术相比，本发明能够简单、高效地制得大面积石墨烯，并且工艺环保、对衬底要求较低、无需催化剂、低温沉积、成本低，因此具有重要潜在的研究应用价值

多通道时间序列谱获取系统

本文介绍了一个基于 IBM PC 机与 CAMAC 的多通道时间序列谱获取系统的原理和实际实现,并进行了详细的性能分析和比较.n this paper,the principle and realization of an acquisition system for multicannel time sequence spectra are discribed.The system is based on IBM personal computer and CAMAC.The detailed performance analysis and comparison show that for the experiments in which the time sequence spectra should be acquired,it has many advantages over the general purpose muhiparameter data aequistion system

用单能γ射线源校刻β塑料闪烁体能谱仪

本文介绍了一种简单、方便而又能精确刻度β塑料闪烁体能谱仪的实用方法.实验使用了~(22)Na、~(137)Cs、~(54)Mn和~(207)Bi等4个γ标准源对β塑料闪烁体能谱仪进行了精确的能量刻度,选取了β能谱仪对单能γ射线响应的康普顿能谱后缘的半高点作为能量参照点.并根据此结果分析处理了~(90)Sr-~(90)Y源的β能谱,其端点能量为2.298±0.90MeV

对称裂变中惯性张量的计算

为了研究核裂变动力学,在假设核是无旋、不可压缩的液滴情况下,采用两参数的广义卡西尼卵形线液滴模型计算了对称裂变中惯性张量.In order to study the dynamics of symmetric fission,the inertia tensor is com- puted based on twopara meter generaelzed Cassinion oval curve dropter model and the assumiption that nucleus is an irrotational and incompressible fluid

新丰中子核素~（185）Hf的研究Ⅱ．合成和鉴别中的快放射化学分离

介绍了在新丰中子核素￣（１８５）Ｈｆ合成和鉴别过程中快放射化学分离流程的研制和实验。用沉淀剂使Ｈｆ生成ＢａＨｆＦ＿６沉淀而与大量的靶物质钨及其他伴随产物分离，所得沉淀制源后，测其γ放射性。整个流程典型分离时间约６－７ｍｉｎ，化学回收率约５３％，去污系数完全满足新丰中子核素￣（１８５）Ｈｆ的合成和鉴别的要求