影响中晚期肝细胞肝癌手术切除预后的多因素分析

探讨影响中晚期肝细胞肝癌手术切除预后的因素。方法对130例中晚期大肝癌随访1～7年,采用单因素、多因素分析统计不同预后因素对患者生存率的影响。结果手术后1,3 ,5年生存率分别81.7%,24.3%,18.4%。单因素分析提示影响预后的因素为肝癌大小、是否早期复发、肝硬化情况、输血量;多因素分析提示肝癌大小、肿瘤早期复发是影响肝癌术后的预后因素。结论：中晚期肝癌手术切除预后仍不理想,重视围手术期处理,预防术后早期复发有望提高手术疗效 [英文摘要]Objective To study the prognostic factors in patients who received hepatectomy for large hepatocellular carcinoma(HCC). Methods 130 patients operated for large HCC were followed up for 1～7 years. Twenty possible factors were analyzed by Kaplan-Meier Log rank estimate. A multivariative survival analysis of these individal variable was undertaken using the cumulative survival rate by the computers COX proportional hazard. Result The overall cumulative survival rate at 1,3,5 years was 81.7%,24.3%,18.4% re..

Potential effects of CRM1 inhibition in mantle cell lymphoma

Fujian Provincial Department of Science Technology [2009-CXB-57/ 2011J01252]; Bureau of Science & Technology of Xiamen, China [3502Z20094012]Mantle cell lymphoma (MCL) is an aggressive histotype of B-cell non-Hodgkin lymphoma. The disease has no known cure, which prompts the urgent need for novel therapeutic agents. Chromosomal region maintenance 1 (CRM1) may play a role in human neoplasia and serve as a novel target of cancer treatment. This study summarizes MCL pathogenesis and determines the involvement of CRM1 in the regulation of several vital signaling pathways contributing to MCL pathogenesis, including the pathways of cell cycle progression, DNA damage response, phosphoinositide kinase-3, nuclear factor-kappa B activation, and chromosomal stability. A preclinical study is also presented to compare the CRM1 status in MCL cell lines and primary MCL cells with normal B cells, as well as the therapeutic efficiency of CRM1 inhibition in MCL in vitro and in vivo, which make these agents potential targets of novel MCL treatments

Research on soil erosion amount and sediment delivery ratio in the hilly red soil region of southern China

依据"层次类剔法"的研究思路,估算了赣江各水文站控制流域的年均侵蚀量与泥沙输移比。结果表明,赣江流域年均侵蚀量1923.0万t/a,平均侵蚀模数237.6t/km2·a;流域内中、低山地貌类型区泥沙输移比大于0.9,丘陵和平原区介于0.5~0.9之间,流域平均为0.54;且土壤侵蚀强度和泥沙输移比分别与流域面积没有规律性的趋势性关系

The scale relationship of sediment discharge,erosion amount and sediment delivery ratio in drainage basin:A case study in the Ganjiang River Basin

输沙量、侵蚀量与泥沙输移比的流域尺度转换研究是当前流域侵蚀产沙研究领域的前沿课题,旨在通过尺度转换理论将坡面小区试验研究成果转换到流域的更大范围。以赣江流域实测输沙量和计算侵蚀量与泥沙输移比数据为基础,探讨了该流域3个变量的流域尺度关系,进而研究分析了3个变量尺度转换的可能性。3个变量与流域面积的关系散点图和相关方程都反映了这3者与流域面积不存在明显的相关关系,相悖于前人反比关系的结论。文章还阐述了流域面积的内涵及输沙量、侵蚀量和输移比的影响因素与流域面积的关系,发现3个变量的影响因素与流域面积不存在尺度效应。由此推断在赣江流域输沙量、侵蚀量和泥沙输移比实现尺度转换存在的可能性不大。这一研究结论是否成立或是否具有普遍性意义还有待于更多流域的研究成果来进一步证实

柔性夹紧装置及抛光设备

本实用新型提供了一种柔性夹紧装置及抛光设备，其柔性夹紧装置安装到抛光设备的本体上，用于夹紧被抛光工件，包括电动缸夹紧器、上法兰盘、下法兰盘、底座板、第一弹性装置和第二弹性装置，电动缸夹紧器固定在上法兰盘上，上法兰盘通过第一弹性装置与底座板连接，下法兰盘通过第二弹性装置与底座板连接，被抛光工件的一端通过电动缸夹紧器夹紧，另一端安装到下法兰盘上。其抛光设备含有上述柔性夹紧装置。本实用新型的柔性夹紧装置及抛光设备，通过第一弹性装置和第二弹性装置使抛光砂轮与被抛光工件保持一种均匀的抛光力进行接触抛光，当抛光力过大时，被抛光工件通过第一弹性装置和第二弹性装置主动回弹避让，防止冲击接触而损伤

Optimization of the Wet Transfer Technique of Graphene

石墨烯的转移过程决定着石墨烯的品质,进而对石墨烯基器件的性能有重要的影响。针对化学气相沉积（CVD）法生长的石墨烯在湿法转移过程中存在的问题,在常规湿法转移的基础上进行了优化研究。实验结果表明：基体背面石墨烯的刻蚀工艺可以有效解决铜箔残留问题;采用二次涂胶工艺可以大幅降低石墨烯的裂痕破洞密度;超声波处理有效提升了石墨烯表面残胶的去除效率。优化后的转移工艺可以明显降低石墨烯产品中的杂质数量和缺陷密度,提升石墨烯的表面洁净度,显著地提高石墨烯质量。The transfer process of graphene plays an important role in determining the quality of graphene,which further exerts an important influence on the performances of graphene-based devices.For resolving the problem in the wet transfer process of graphene grown by the chemical vapor deposition（CVD）method,the optimized procedure was researched based on the conventional wet transfer of graphene.The experimental results show that the residue of copper is effectively eliminated by an extra etching of the graphene deposited on the back of copper.The densities of surface cracks and holes are largely reduced with the two-step coating process.The removal efficiency of the residual polymer on the surface of graphene is significantly enhanced by the ultrasonic treatment.The optimized transfer procedure can obviously reduce the amount of impurities and defect density of graphene and significantly improve the surface cleanliness and quality of graphene.厦门市科技项目（3502Z20143003

Preparation of Single Layer Graphenes via an Optimized CVD Method

作为碳原子家族的最新成员,二维结构的石墨烯具备优异的物理化学性质和广阔的应用前景,成为新材料研究领域的热点研究对象。对化学气相沉积（CVD）法进行了优化,成功制备得到了高质量的单层石墨烯。优化后的实验工艺为：首先对铜箔进行化学抛光和退火预处理;然后将预处理后的铜箔加工成荷包状;氢气（H2）氛围下,以甲烷（CH4）为碳源,对石墨烯进行化学气相沉积;当甲烷和氢气体积流量分别为10 cm3/min和20 cm3/min时,在1 030℃条件下生长20 min制备得到最终样品。扫描电子显微镜（SEM）和激光喇曼光谱表征的结果显示：该方法制备的样品为大面积连续的单层石墨烯。As the latest member of the carbon family,the graphene with two-dimensional structure becomes a hot research subject in the new material research field due to its outstanding physical and chemical properties and wide application prospect.The single layer graphene with high quality was prepared successfully by the optimized chemical vapor deposition（CVD）method.In the optimized experiment process,the chemical polishing and annealing pre-treatment of the Cu foil were carried out firstly,then the pre-treated Cu foil was fabricated into a cavity shape;the CVD growth of the graphene was conducted with the methane（CH_4）as the carbon source under the hydrogen（H_2）atmosphere.The final samples were prepared at 1 030 ℃for 20 min with the CH_4 and H_2volume flow rates of 10 cm^3/min and 20 cm^3/min,respectively.The results characterized by the scanning electron microscope（SEM）and Raman spectroscopy show that the single layer graphene with high quality and large area can be obtained via this optimized CVD procedure.厦门市科技项目（3502Z20143003

Role of Notch expression in premature senescence of murine bone marrow stromal cells

National Natural Science Foundation of China [30570773]The aim of the present study was to investigate the role of the Notch signaling pathway in premature senescence of murine bone marrow stromal cells in vitro. The intracellular domain of Notch 1 (ICN) was transfected into cultured murine bone marrow stromal cells by lipofectamine transfection. After three days, the proliferation of transfected cells was measured by MTT assay. Cell cycle distribution was analyzed by flow cytometry. Senescence-associated beta-galactosidase (SA-beta-gal) was measured, and the percentage of positive cells was evaluated by assessing 1000 cells in random fields of view. The expressions of p53 and p21(Cip1/Waf1) were analyzed by both RT-PCR and Western blot analysis. The results showed that activation of Notch signaling inhibited proliferation of murine bone marrow stromal cells with induction of G(1) arrest, increased the percentage of SA-beta-gal positive cells, and upregulated p53 and p21(Cip1/Waf1) mRNA and protein expression levels. Thus, the activated Notch signaling could induce premature senescence of bone marrow stromal cells through the p53-p21(Cip1/Waf1) pathway. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved

Parthenolide induces apoptosis and lytic cytotoxicity in Epstein-Barr virus-positive Burkitt lymphoma

Fujian Provincial Department of Science and Technology [2009-CXB-57, 2011J01252]; Bureau of Science and Technology of Xiamen, China [3502Z20094012]Burkitt lymphoma (BL) has been reported to be strongly associated with Epstein-Barr virus (EBV) infection. The fact that EBV is generally present in cancer cells but rarely found in healthy cells represents an opportunity for targeted cancer therapy. One approach is to activate the lytic replication cycle of the latent EBV. Nuclear factor (NF)-kappa B is thought to play an essential role in EBV lytic infection. Elevated NF-kappa B levels inhibit EBV lytic replication. Parthenolide (PN) is a sesquiterpene lactone found in medicinal plants, particularly in feverfew (Tanacetum parthenium). The aim of the present study was to analyze the effect of PN on the survival of Raji EBV-positive lymphoma cells. Raji cells were treated with 0,4 or 6 mu mol/l PN for 48 h. MTT assay and western blot analysis were performed to evaluate the findings. Results showd that PN suppressed the growth of the EBV-positive BL cell line, Raji, and activated the transcription of BZLFI and BRLFI by inhibiting NF-kappa B activity. Most notably, when PN was used in combination with ganciclovir (GCV), the cytotoxic effect of PN was amplified. These data suggest that the induction of lytic EBV infection with PN in combination with GCV may be a viral-targeted therapy for EBV-associated BL