High-throughput Analysis of Protein−Protein Interaction by High Sensitivity Flow Cytometry Combined with GFP Reporter

蛋白质相互作用伴随着生命活动的整个过程，是任何生命体结构和生命活动的基础。研究蛋白的相互作用有助于蛋白质功能的分析、相互作用网络的构建以及药物的开发等问题的解决。人们通常采用免疫共沉淀法、表面等离子体共振法以及荧光共振能量转移等方法研究蛋白的相互作用，然而这些方法通常在体外进行试验，需要对蛋白质进行提取、纯化等繁琐步骤，在一定程度上影响了蛋白的正常生理状态，不能反映蛋白的真实情况。近年来随着生物技术的发展，酵母双杂交系统得到了广大研究者的青睐，该系统采用分子生物学的方法将所需研究的蛋白质基因片段构建到相应的质粒中，蛋白质在细胞内表达，通过相互作用激发报告基因的转录表达，进而通过分析报告基因来判...Protein interaction, involoved in the whole process of life activity, is the foundation of any living body structure and life activity. Study on the interaction of proteins is of great significance for the analysis of protein function, interaction network construction, drug development and other issues to be solved. Generally, protein interaction is studied by immunoprecipitation, surface plasmon ...学位：工学硕士院系专业：化学化工学院_化学工程学号：2062014115148

最大后验贝叶斯估算法制定华法林个体化给药案例探讨

目的:探讨临床药师在华法林个体化给药方案制定及实施监护过程中发挥的作用。方法:临床药师采用最大后验贝叶斯估算法制定华法林患者的给药方案,并对方案实施过程进行了监护,及时识别了药物相互作用导致的INR升高,避免了出血不良反应的发生,同时也及时发现了患者漏服药物导致的国际标准化比值(INR)降低,避免了患者再次发生血栓。结果:最大后验贝叶斯估算法可以较好地估算和指导临床用药,对于个体化给药方案具有极大的参考价值。结论:根据基因检测结果选择合适的算法为华法林患者制定了个体化给药方案后,临床药师还需要对整个给药方案实施过程提供全程化的监护,以便及时发现疾病、药物相互作用、依从性不佳等导致的INR异常波动,从而提供抗凝效果,减少出血和栓塞等不良反应。福建省科技计划引导性项目(编号:2015Y0020

厦门地区非饱和残积土抗剪强度的试验研究

非饱和残积土是厦门地区诸多填方工程的工程用土,而岩土工程的强度与稳定性问题都涉及到非饱和土的抗剪强度及其指标.采用滤纸法测定非饱和残积土的基质吸力,通过一系列的直接剪切试验,测定不同初始含水量状态下土的抗剪强度及其强度指标.试验结果表明:基质吸力随着含水量、体积含水量或饱和度的增加而减小.当含水量小于16%,体积含水量大于35%或者饱和度大于70%时,基质吸力对含水量的变化非常敏感.在低基质吸力区,抗剪强度随着基质吸力的增加显著增加,表观粘聚力随基质吸力的增大而线性增加;在高基质吸力区,强度随基质吸力的增加变化不大,表观粘聚力的增长速率减小.表观内摩擦角仅在基质吸力较小时增加而后可近似为某一定值

南海中深层动力格局与演变机制研究进展

南海是连接印度洋-太平洋的最大边缘海,在季风、海峡水交换以及复杂地形影响下,南海环流呈现出独特的三层结构以及远强于大洋的混合特征.理论与观测表明,南海内潮、内孤立波以及强风等过程是强混合的动力来源.在南海强混合作用下,南海发育了活跃的中深层动力系统,一方面促进了南海与大洋之间的水体交换,另一方面调控上层风生环流,使得南海环流显著区别于其他热带与副热带海盆.南海活跃的中深层环流所具有的物质搬运能力又显著影响着南海的地质沉积、生物地球化学循环等过程.中国对深海研究持续投入,在南海中深层环流动力学研究方面取得了显著的成果,文章就该方面进行总结,并对南海深海环流未来研究设想进行初步探讨

A New Random Walk Simulation Model for Study of Diffusion Behavior of Single Particle Within Two-Dimensional Space

分子的扩散行为是微观化学的重要研究领域. 影响扩散行为的因素很多，但是目前各个因素的具体影响效果还不明确. 作者基于随机行走理论建立了分子在二维空间的扩散模型，依据此模型自主开发了模拟软件以及数据分析系统，并利用该模拟软件系统研究了势垒尧横向速度等因素对扩散行为的影响，验证了该模型的可靠性，证明根据该模型可以得到和实验尧理论相吻合的结果. 该软件有望成为模拟微观化学扩散行为的潜在平台，如电化学以及膜过滤过程中的扩散.Research on diffusion behaviors is of significant value in that it is closely related to transport phenomena in micro-chemistry. However, the effects of variables on diffusion are still unclear. Here, we developed and programmed a simulation methodology along with data analysis, which was capable to simulate the diffusion of a particle within twodimensional heterogeneous space in large timescale; the effects of periodically arranged impenetrable barriers of specific shape and lateral drifting velocity on diffusion behavior were studied. As well as standard mean square displacement analysis, a new method, the appearance probability distribution method, was introduced, which revealed whether the particle tended to be present at certain positions. This article introduced the construction of the simulation model and demonstrated the validity of the model. The results showed that our model fit qualitatively well with experiments and theories. The model was proved to be an excellent potential platform for simulating the diffusion behaviors in micro-chemistry, such as the diffusion process in electrochemistry as well as nanofiltration membrane.This work was supported by National Basic Research Program of China (973 Program, 2010CB732400), the National Natural Science Foundation of China (NSFC) (20821063, 20873063, 51071084, and 21273113), the Natural Science Foundation of Jiangsu Province (BK2010389).This work was supported by National Basic Research Program of China (973 Program, 2010CB732400), the National Natural Science Foundation of China (NSFC) (20821063, 20873063, 51071084, and 21273113), the Natural Science Foundation of Jiangsu Province (BK2010389).作者联系地址：南京大学 化学化工学院, 生命分析化学国家重点实验室, 江苏 南京 210008Author's Address: State Key Lab of A nalytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210008, China通讯作者E-mail：[email protected]

analysis of fault location in software compatibility testing

为了有效降低软件兼容性测试的测试成本,提高测试精度和简化测试过程,设计了一种基于组合测试的建模方案。基于该建模方案,提出了一种软件兼容性测试的故障定位分析方法。该方法首先针对需要进行兼容性测试的软件集建立组合测试模型;然后用组合测试用例生成工具Cascade对该模型生成测试用例集,并执行这些测试用例集得到结果;最后将这些测试用例及其执行结果作为组合测试错误定位工具Facil的输入,从而精确定位出发生兼容性错误的软件组合。实验表明,该方法步骤简捷,结果有效、可靠,在一定程度上降低了测试成本,提高了测试精度,简化了测试过程。国家自然科学基金No.60903049This paper proposes a new model for fault location about software compatibility testing based on combinatorial testing in order to lower cost, improve accuracy and simplify process during test. Based this model, this paper proposes a method to analyze fault location in compatibility testing. First of all, the model is built for softwares which need to do compatibility testing. After that, the tool Cascade is used to generate test cases for this model which are executed and get results. Finally, these test cases and execution results are input as the tool of Facil which is used for fault location about combinatorial testing. Facil will precisely find software set which causes the compatibility fault. Experiments show that the process of this method is simple and the result is effective and reliable. To some extent, it lowers cost, improves accuracy and simplifies process during test