Determination of Glycyrrhizin and Liquiritin in Licorice(Glycyrrhiza) by HPLC Method

目的测定甘草中甘草酸和甘草苷的含量。方法采用反相高效液相色谱法,DENALIC18色谱柱(VYDAC238DE5415,120A,5μm,4.6 mm×150 mm),梯度洗脱,流动相A∶H2O,流动相B∶1.5%HAc-M eOH,流速1.0 m.lm in-1,检测波长在32.2 m in从330 nm换为252 nm,从35 m in换为330 nm,柱温40℃。结果甘草苷的浓度在10~50μg.m l-1之间与峰面积线性关系良好,平均回收率为99.97%,方法精密度RSD=0.12%(n=5),甘草酸的浓度在50~90μg.m-l1之间与峰面积线性关系良好,平均回收率为100.25%,方法精密度RSD=0.13%(n=5)。结论该方法可用于甘草中甘草酸和甘草苷的同时含量测定。ObjectiveTo determine the content of glycyrrhizin and liquiritin in licorice(Glycyrrhiza).MethodsRP-HPLC method was estabilished.The analytical column was DENALI C18(VYDAC 238DE5415,120A,5μm,4.6 mm×150 mm),and gradient elution with A:H_2O,B:1.5% HAc-MeOH as mobile phase.The flow-rate was 1.0 ml·min~(-1).The detection wavelength was from 330nm to 252 nm at the 32.2 min,and then returned to 252 nm at the 35 min.The column temperature was 40℃.ResultsThe content of liquiritin was good linear related to its peak area in range of 10～50 μg·ml~(-1),and the average recovery of the method was 99.97%,the RSD of precision test was 0.12%(n=5).The content of glycyrrhizin was good linear related to its peak area in range of 50～90 μg·ml~(-1),and the average recovery of the method was 100.25%,the RSD of precision test was(0.13)%(n=5).ConclusionThe method can be used for determing the content of glycyrrhizin and liquiritin in licorice at the same time.国家自然科学基金2003重点项目(No.20235020

Discussion on the Feasibility Applied Fingerprint Chart in Quality Control of Chinese Herbs Pieces

　为促进中药饮片质量的研究,通过对文献资料的归纳、分析,对应用各种方法建立中药标准炮制品的指纹图谱库,并以指纹图谱相似度来评价中药饮片质量的可行性进行综合评述。中药指纹图谱在实现对中药饮片进行科学、客观、公正、有效的质量控制方面将起到重要的作用。In order to promoting the quality research of Chinese herbs pieces, the authors summed up and analyzed many literature information, and synthetically commented the feasibility applied the similarity of fingerprint chart in quality control of Chinese herbs pieces. It would show a important effect in impersonally and effectively controlling the quality of Chinese herbs pieces

HPLC指纹图谱应用于炙甘草的炮制研究

目的:应用HPLC指纹图谱规范炙甘草的炮制。方法:采用反相高效液相色谱法,DENALI C18色谱柱(VYDAC238DE5415,120A,5μm,4.6mm×150mm),以3%(v/v)HAc溶液和甲醇为流动相,梯度洗脱,柱温40℃,检测波长252nm。结果:炙甘草的HPLC指纹图谱有20个共有峰。传统炒制法炮制的炙甘草样品有一部分峰的重现性RSD值>3%;烘制法炮制的样品重现性有2个共有峰相对峰面积比的RSD>3%;微波法炮制的样品重现性符合指纹图谱的要求,20个共有峰相对峰面积比的RSD值均小于3%。结论:烘制和微波加热的炮制方法,可控性强,样品的指纹图谱重现性高,可以作为替代传统炒制工艺的新工艺。国家自然科学基金2003重点项目(20235020

黄土高原聚湫沉积旋回、土壤侵蚀及区域差异

黄土高原聚湫/淤地坝内的沉积物是黄土再侵蚀搬运的直接结果,以高堆积速率和多沉积旋回为特征,是认识黄土高原地球关键带过程和历史的理想载体之一。如何鉴定并有效划分沉积旋回是利用聚湫/淤地坝沉积序列恢复流域土壤侵蚀和地表环境过程的基础。本文选取地处黄土高原中部丘陵沟壑区(陕西靖边)和高塬沟壑区(甘肃合水)的两个聚湫为代表,利用高分辨率XRF岩芯扫描仪获取两个聚湫上部近代沉积序列的元素分布,提出划分沉积旋回的地球化学指标,并识别年际冻融层,获得旋回产沙模数及年际产沙量,进而讨论土壤侵蚀与强降水频率和强度的关系及区域差异。结果表明,沉积旋回的下部粗颗粒层均以高Zr为特征,上部细颗粒层则富集Al、Fe、Rb、Ca和Sr等造岩元素,由此提出Rb/Zr比值可有效划分沉积旋回。根据Rb/Zr比值分布,靖边和合水聚湫上部4m和2m沉积序列可分别划分为35个和18个沉积旋回。结合细颗粒层的~(137)Cs比活度分布,划分的沉积旋回个数可分别与1963年以来两个地区强降水次数一一对应。靖边聚湫的年际沉积旋回数量和产沙模数均高于合水,是黄土高原丘陵沟壑区砂黄土更易遭受侵蚀的结果;在次降水量为50mm的强度下,靖边流域的产沙模数约为合水流域的两倍左右。重要的是,两个流域的产沙模数均与强降水次数和强度紧密相关,旋回产沙模数随降水强度线性增加。聚湫沉积旋回记录的侵蚀历史及区域差异将有助于深入认识黄土高原地球关键带中侵蚀风化、水文和生态环境变化等历史与过程。</p