Research on preparation of silica based vectors and transfection in vivo

本文旨在以硅氧烷纳米材料作为基础，引入阳离子聚合物，制备有机-无机杂化纳米基因载体。本文工作主要分为以下三个方面：1.以羧甲基壳聚糖、有机硅烷（GPSM）作为反应物，以氨水作为反应介质，在水相中制得羧甲基壳聚糖-硅氧烷（CMG）纳米颗粒。采用电子透射显微镜及粒径仪对CMG纳米颗粒的形貌、尺寸和表面电位进行表征。通过红外光谱、热失重分析等方法，对CMG纳米颗粒的结构进行了分析，并探讨了该基因载体的合成机制。为了提高该载体的功能性及结合DNA的能力，通过共价键连的方法将具有逃逸溶酶体功能的KALA活性多肽修饰到CMG纳米颗粒表面（CMG-KALA）。通过激光共聚焦、荧光光谱以及红外光谱证明KALA...This paper was aimed to prepare organic-inorganic hybrid nano-gene vector based on silica nano materials and cation polymers for gene delivery. The following three aspects were discussed in this paper:1. CMG nanoparticles were synthesized through a rapid one-step aqueous synthetic approach with carboxymethylchitosan, GPSM and ammonia system. The morphology, size and surface potential were characte...学位：理学硕士院系专业：材料学院生物材料系_生物医学工程学号：2452006115267

电沉积钙磷盐涂层及其影响因素的研究

发展在低温下电沉积钙磷盐的方法,制备了可以提高医用钛表面生物活性的涂层。考察了电流密度和温度的变化对涂层性质和形貌的影响,并应用扫描电镜(SEM)、X射线衍射(XRD)、傅立叶-红外光谱(FT-IR)对合成的生物材料进行分析和表征,探明了电沉积钙磷盐涂层主要因素的影响作用。结果表明,反应温度可导致钙磷盐涂层的形貌和组成的变化,电流密度则影响钙磷盐晶体的生长方向

Synthesis and cellular uptake mechanism of Tat decorated Au-Au_2S nanoparticles

［中文文摘］为了实现对肿瘤的靶向性药物/基因治疗,通过化学还原法制备了细胞穿膜肽Tat修饰的Au-Au2S纳米药物载体。采用透射电镜、表面增强拉曼光谱仪、紫外分光光度计对Tat/Au-Au2S纳米粒子进行表征,采用流式细胞仪、激光共聚焦显微镜研究Tat/Au-Au2S纳米粒子的穿细胞膜机制。理化分析结果表明,Tat可通过Au—S键接枝于Au-Au2S纳米粒子表面,直径约50 nm的Tat/Au-Au2S纳米粒子具有近红外敏感性。细胞内化途径示踪物共定位分析和抑制剂阻断实验表明,Tat/Au-Au2S纳米粒子以脂筏介导的巨胞饮途径进入Hela细胞,而以受体和脂筏共介导的巨胞饮途径进入骨髓间充质干细胞(BMSCs)。［英文文摘］　To realize an efficient and targeted cancer therapy by the drug/ gene carrier , cell penet rating peptide Tat decorated Au2Au2 S nanoparticles were prepared by a redox method. Transmission elect ron microscopy ( TEM) ,surface2enhanced Raman scattering ( SERS) and UV2vis spect rometer were used for characterizing Tat/ Au2Au2 S nanoparticles , and confocal laser scanning microscope (CLSM) and flow cytometer (FACS) were used to investigate the mechanism of cellular uptake. The chemicophysical result s indicate that Tat peptide could be conjugated onto Au2Au2 S nanoparticles via Au —S bonds , and Tat/ Au2Au2 S nanoparticles present as 50 nm2 diameter sphericities with NIR sensitivity. Co 2location and endocytosis inhibition experiment s suggest that Tat/ Au2Au2 S nanoparticles may enter Hela cells via a lipid raf t mediated endocytosis pathway , whereas via a combined endocytosis pathway of lipid raf t2dependent and receptor2dependent into bone marrow st romal cells (BMSCs).973计划项目(2007CB935603); 国家自然科学基金(30670559);福建省自然科学基金(2006J0121);福建省新世纪优秀人才计划项目

Engineering practice of the treatment of wastewater from flexible printed circuit board factories

介绍了厦门某电子科技有限公司废水处理工程实例。针对该线路板厂废水来源的不同,依据分类收集、先预处理再综合处理的原则对废水进行合理的细化分类,对不同的水质进行不同的物化处理。工程试车结果表明,采用本工艺路线处理后的柔性印制电路板废水出水水质达到或优于GB8978—1996一级排放标准。An example of the engineering practice of the wastewater treatment at an electronic technology company in Xiamen is introduced. Considering the different characteristics of wastewater sources at the circuit board factory, and based on classifying the collections, the principle of classifying the wastewater is followed and the wastewater is treated properly and detailedly by pre-treatment firstly and then by comprehensive treatment. Wastewater with different water quality is treated by different physicochemical treatment. The practice results show that when flexible printed circuit board wastewater is treated by this process,the effluent water quality meets or superior to the requirement of the first class of the standard,GB 8978—1996

Electrophoretic Deposition of HA/CNTs Composite Coating

采用电泳沉积的方法在医用金属钛表面沉积纳米羟基磷灰石(HA)/多壁碳纳米管(MWCNTs)复合涂层.力学拉伸实验表明,所制备的纳米HA与MWCNTs的复合涂层可显著增强其与基底材料的结合力.体外细胞培养试验证明,电泳沉积制备的复合涂层具有良好的生物相容性.可望成为一种新的综合性能良好的硬组织生物材料.In order to enhance the bonding strength of hydroxyapatite (HA) coating to a metal substrate,and also improve its biocompatibility,the electrophoretic deposition(EPD) technique has been developed to prepare a composite coating of HA/multi-walled carbon nanotubes(MWCNTs) on a titanium substrate.It is indicated that after sintering at 700℃ for 2 h the adhesive strength of the prepared composite coating to the titanium substrate is increased as high as 35.44 MPa.And it is also demostrated,in vitro cell culture test,that the HA/MWCNTs composite coating is of a good biocompatibility.It is promising to develop a new hard tissue biomaterial with satisfied mechanical and biological properties.国家自然科学基金(20273055);; 教育部博士点基金(20020384012);; 国家863计划(2003AA302230);; 福建省科技重大项目(2003I001)资

Effect of the etching treatments on titanium surface morphology

发展电化学刻蚀和化学刻蚀技术,对钛表面进行处理,并应用扫描电镜、X射线衍射方法对其表面进行表征,探讨电化学刻蚀钛表面形成微观结构的机理。结果表明,经刻蚀后钛表面形成了纳米级微观结构,提高了表面粗糙度,可增强生物材料涂层与钛基底的结合强度。In this work,the techniques of the electrochemical etching and the chemical etching have been developed for the titanium surface modification.The etched titanium surfaces were characterized by scanning electron microscopy(SEM) and X-ray diffraction(XRD).The results indicated that microporous microstructures were formed on the titanium surfaces,which was beneficial for increasing the adhesion strength between the titanium surface and other biomaterial coatings.国家自然科学基金资助项目(20273055);; 国家高技术研究发展计划(863计划)资助项目(2003AA302230

Sediment transport patterns in the eastern Beibu Gulf based on grain-size multivariate statistics and provenance analysis

通过对北部湾东部海域表层底质样的粒度和重矿物分析,获得了粒度和矿物组成特征。对粒度进行因子分析获得了4个主控因子,其中因子2和3分别代表细颗粒的悬移质组分和陆源粗颗粒推移质组分,指示来自琼州海峡和北部湾西岸、西北岸的物源贡献,而且因子2的高得分区与弱环流背景相吻合;因子4反映了粤西和海南岛东岸沿岸流的物源贡献。采用重矿物组合,将研究区沉积物的物质来源划为4个分区。分别对4个物源分区使用粒径趋势分析模型,把它们合并后获得了整个研究区的趋势矢量,其中海湾北部沉积物输运汇聚中心的位置与余环流的中心位置基本一致,海南岛西部海域沉积物显示为向北输运的特征,与地貌、水动力特征基本吻合,在一定程度上可消除物源混合造成的影响。在使用粒径趋势分析模型获取沉积物净输运方向时,需要综合考虑物源差异和空间尺度对沉积物粒径趋势的影响。Sediment samples collected from the eastern Beibu Gulf were analyzed in the laboratory to obtain grain-size distributions and mineral components.Four main control factors are obtained using a factor analysis.Factor 1 is contributed by 2.5Φ-3.75Φ and 5.75Φ-7.75Φ.Factor 2 respects fine suspended load component,indicating that the provenance contribution from the Qiongzhou Strait and the high score area is consistent with the weak circulation.Factor 3 is a indicator of terrigenous coarse bedload component from the west and northwest coast of the Beibu Gulf.Factor 4 can reflect the transportation by the coastal current along the east coast off Hainan Island and the west of Guangdong Province.The study area can be separated into four subareas of the sediment sources on the basis of heavy mineral analysis.A grain-size trend analysis model is separately used for the four subareas to estimate the net sediment transport pathways,which can remove the affects induced by the sediment mixing from different sources.A converge center of grain-size trend vectors in the area of the west of Hainan Island might result from the mixing of different sources of sediments,instead of sediment transport.Actually,the northward transport dominates this area of the west of Hainan Island.Another converge center in the northern area is well agreement with the local geomorphology and large-scale ocean circulation pattern.Thus,the application of grain-size trend should consider the effect of sediment sources and spatial scales in order to derive the reasonable net sediment transport pathways.国家和海南省“九2八”专项资助(908-01-ST09;HN908-02-05);国家大学生创新训练计划项目资助(061028405);国家基础科学人才培养基金项目资助(J0630535

The Application of Geostatistics to Analysis of Grain Size Trend in the Eastern Beibu Gulf

在北部湾东部海域采集表层沉积物71个,通过粒度分析获得其粒度参数(平均粒径、分选系数、偏态),用克里格插值法将不规则采样站位的粒度参数内插为规则网格分布的相应粒度参数。采用地统计法分析其空间相关性,计算度量空间相关性范围的参数,即半方差图中的变程值。结果表明,使用地统计分析获得的粒度参数变程值物理意义较为明确,可作为粒径趋势分析模型的特征距离,其中分选系数变程值作为特征距离的计算结果与前人的海流、沉积物输运信息更为吻合;这在一定程度上消除了传统方法(试算法或经验估计法)获取特征距离可能造成的模型计算误差。采用不同间距插值时得到的粒径趋势矢量具有不同的空间分辨率,其中高分辨率的细化图所反映的海底沉积物净输运趋势与余流和环流等所反映的沉积物输运细节特征吻合较好,低分辨率的概化图可大致反映该区域沉积物的总体输运趋势。地统计分析的结果对未来研究工作中采样间距的选取也具有指导意义。A total of 71 surficial sediment samples are collected in the eastern Beibu Gulf, and then the moment parameters (i.e. mean size, sorting coefficient and skewness) are obtained by grain size analysis. By using kriging interpolating methods, the regular meshes of grain size parameters are calculated. A geostatistic analysis is applied to study the spatial autocorrelation for these parameters; while the range, a parameter in the semivariogram metering the bound of the spatial autocorrelation, is estimated. It shows that the range for sorting coefficient is physically meaningful, and also the obtained grain size trends are consistent with the annual ocean circulation pattern and sediment transport rates from previous studies. Thus, the range from the sorting coefficient variogram can be used as the characteristic distance in the grain size trend analysis model, which may remove the bias caused by traditional determination of characteristic distance (e.g. based on experience or testing methods). The sediment transport patterns are retrieved on the basis of grain size parameters interpolated with varied distances, which indicates that the small interpolation distance can produce better transport pattern with high resolution and give more detailed information. Hence, the results from geostatistic analysis can be guidable in determination of sampling stations density in the future field work.国家908专项(908-01-ST09);; 国家大学生创新训练计划项目(SIT-05);; 教育部“新世纪优秀人才支持计划”(NCET-06-0446);; 国家基础科学人才培养基金(J0630535)~

水合氧化锆的晶化方式对Pt/WO_3-ZrO_2异构化活性的影响

采用不同方法制备了一系列具有一定晶相结构的水合ZrO2,考察了制备方法对水合ZrO2晶化方式及Pt/WO3-ZrO2催化剂催化正己烷异构化活性的影响.采用X射线衍射,Raman光谱和NH3-程序升温脱附对催化剂进行了表征.结果表明,水合ZrO2的晶化方式与制备时氧气的存在与否有关,也大大影响了催化剂的异构化活性.以无氧条件下制备的水合ZrO2为载体时,Pt/WO3-ZrO2催化剂具有较高的异构化活性,而以在空气气氛中制得的水合ZrO2为载体时,Pt/WO3-ZrO2几乎无异构化活性