抗SARS-CoV N蛋白单克隆抗体的特异性及其识别抗原表位的初步鉴定

为了明确抗SARS-CoVN蛋白单克隆抗体的特异性,并鉴定其识别表位,首先在E.coli中表达了人类冠状病毒229E(HCoV-229E)和OC43(HCoV-OC4)N蛋白,用Westernblotting和间接免疫荧光方法分别检测了4株抗SARS-CoVN蛋白单克隆抗体(1-1C2、1-1D6、2-8F11和2-2E5)与HCoV-OC43和HCoV-229E及其N蛋白的交叉反应情况,而后应用12种重组截短型SARS-CoVN蛋白对上述4种单克隆抗体的识别表位进行了初步定位。结果显示:(1)在4株抗N蛋白单克隆抗体中,1-1C2、1-1D6和2-2E5不与HCoV-OC43和HCoV-229E及其N蛋白发生交叉反应,为SARS-CoVN蛋白特异性抗体;(2)2-8F11、1-1D6和2-2E5针对的抗原表位位于SARS-CoVN蛋白的aa30-60,1-1C2针对的抗原表位则位于SARS-CoVN蛋白的aa170-184。这一研究为阐明SARS-CoVN蛋白的免疫学特征,建立特异性免疫诊断技术和研究其致病机制提供了必要的依据和材料

Numerical simulations on thermocapillary migrations of nondeformable droplets with large Marangoni numbers

In this paper, the study on isolated spherical drops in thermocapillary migrations in zero gravity is carried out with a novel numerical scheme to accomplish long-tank simulations in a very short computing domain, and the full migrating phenomena with fairly large Marangoni numbers (up to 400) are discussed in detail. Larger Marangoni numbers lead to more complicated migrating processes, and longer distances for the drops to reach their final stable migrating velocities (U-F). There is nontrivial difference between the U-F values in theoretical analysis, numerical simulations, and space experiments, and the most possible reason is the assumed different migrating distances to reach steady states in different investigations

不变形双滴的热毛细迁移及相互作用

对微重力下不变形双滴的非定常热毛细迁移运动进行了数值模拟,采用了有限差分方法对动量方程和能量方程进行离散,使用波前追踪法捕捉运动的不变形液滴界面.研究显示双滴的排列方式对它们的迁移规律和相互作用影响很大,其中影响任一个液滴运动的最主要的因素是另一个液滴的存在所引起的温度场的扰动

Thermocapillary Migration and Interaction of Two Nondeformable Droplets

对微重力下不变形双滴的非定常热毛细迁移运动进行了数值模拟,采用了有限差分方法对动量方程和能量方程进行离散,使用波前追踪法捕捉运动的不变形液滴界面.研究显示双滴的排列方式对它们的迁移规律和相互作用影响很大,其中影响任一个液滴运动的最主要的因素是另一个液滴的存在所引起的温度场的扰动

Soil Quality Evaluation Under Different Land Use in Southern Ningxia Mountain Area

运用层次分析法对研究区域的土壤进行了较为全面、较为科学的评价依据隶属函数确定了各评价指标的隶属度,实现了原始数据的归一化处理;同时也利用主成分分析法确立了各评价因子的权重分配问题。通过对研究区域土壤质量的评价,充分表明了土地利用方式不同对于土壤的改良效果存在明显差别灌木林地>天然草地>农地>人工草地>果园

Study on the pedogenesis characteristics and soil taxonomy in the wind-water erosion crisscross zone of north of Shaanxi

对陕西北部神木、榆林、定边等县区 11个典型剖面的分析研究表明 ,本地区土壤存在不同程度的退化现象 ,表现为土壤机械组成粗化 ,有机质含量较低 ,氮磷营养成分贫乏 ,土壤交换性能较差等。在系统分类中共检索出 5个土纲 ,7个亚纲 ,11个土类。发生分类与系统分类结果之间既存在着差异 ,也有一定的对应关系

脂肪醛去甲酰加氧酶(ADO)的酶学及结构-功能关系研究

脂肪醛去甲酰加氧酶(aldehyde-deformylating oxygenase,ADO)催化脂肪醛转化为脂肪烃,是蓝细菌脂肪烃合成途径中的一个关键酶,属于类铁蛋白超家族。研究其结构-功能关系,对拓宽类铁蛋白超家族的催化多功能性以及构建高效合成脂肪烃类生物燃料的基因工程蓝细菌具有重要的意义

Thermocapillary Migration Of Nondeformable Drops

In this paper, we present a numerical study on the thermocapillary migration of drops. The Navier-Stokes equations coupled with the energy conservation equation are solved by the finite-difference front-tracking scheme. The axisymmetric model is adopted in Our simulations, and the drops are assumed to be perfectly spherical and nondeformable. The benchmark simulation starts from the classical initial condition with a uniform temperature gradient. The detailed discussions and physical explanations of migration phenomena are presented for the different values of (1) the Marangoni numbers and Reynolds numbers of continuous phases and drops and (2) the ratios of drop densities and specific heats to those of continuous phases. It is found that fairly large Marangoni numbers may lead to fluctuations in drop velocities at the beginning part of simulations. Finally, we also discuss the influence of initial conditions on the thermocapillary migrations. (C) 2008 American Institute of Physics