Determination of amino acids in Alisma orientale by pre-column derivatization of RP-HPLC

目的　分析不同产地泽泻中氨基酸含量及其特征性。方法　采用邻苯二甲醛 (OPA)、氯甲酸芴甲酯(FMOC)联合柱前衍生RP HPLC测定泽泻中的 17种氨基酸。结果　氨基酸质量浓度与峰面积呈良好的线性关系 ,r均在 0 99以上。不同产地泽泻中氨基酸含量有一定差别。结论　该方法适宜泽泻药材中氨基酸含量的测定。Object To determine the contents of amino acids a nd study the characters of Alisma orientale (Sam. Juzep.) from d ifferent habitats. Methods The samples of A. orientale from thr ee different areas were derived with o-phthaldialdehyde (OPA) a nd 9-fluorenylmethyl chloroformate (FMOC) in pre-column, and separated by RP- HPLC. Seventeen kinds of amino aci ds were determined. Results The concentration of amino acids and peak areas achieve d a nice linear relations (r is higher than 0.99). There were differences of the amino acid in A. orientale from diffe rent habitats. Conclusion This method is suitable for the determination of am ino acid in A. orientale.国家自然科学基金 2 0 0 3重点项目 ( 2 0 2 3 5 0 2 0 ) ;; 福建省泽泻GAP研究项目 ( 2 0 0 2Y0 2 4

Determination of Glycyrrhizin and Liquiritin in Licorice(Glycyrrhiza) by HPLC Method

目的测定甘草中甘草酸和甘草苷的含量。方法采用反相高效液相色谱法,DENALIC18色谱柱(VYDAC238DE5415,120A,5μm,4.6 mm×150 mm),梯度洗脱,流动相A∶H2O,流动相B∶1.5%HAc-M eOH,流速1.0 m.lm in-1,检测波长在32.2 m in从330 nm换为252 nm,从35 m in换为330 nm,柱温40℃。结果甘草苷的浓度在10~50μg.m l-1之间与峰面积线性关系良好,平均回收率为99.97%,方法精密度RSD=0.12%(n=5),甘草酸的浓度在50~90μg.m-l1之间与峰面积线性关系良好,平均回收率为100.25%,方法精密度RSD=0.13%(n=5)。结论该方法可用于甘草中甘草酸和甘草苷的同时含量测定。ObjectiveTo determine the content of glycyrrhizin and liquiritin in licorice(Glycyrrhiza).MethodsRP-HPLC method was estabilished.The analytical column was DENALI C18(VYDAC 238DE5415,120A,5μm,4.6 mm×150 mm),and gradient elution with A:H_2O,B:1.5% HAc-MeOH as mobile phase.The flow-rate was 1.0 ml·min~(-1).The detection wavelength was from 330nm to 252 nm at the 32.2 min,and then returned to 252 nm at the 35 min.The column temperature was 40℃.ResultsThe content of liquiritin was good linear related to its peak area in range of 10～50 μg·ml~(-1),and the average recovery of the method was 99.97%,the RSD of precision test was 0.12%(n=5).The content of glycyrrhizin was good linear related to its peak area in range of 50～90 μg·ml~(-1),and the average recovery of the method was 100.25%,the RSD of precision test was(0.13)%(n=5).ConclusionThe method can be used for determing the content of glycyrrhizin and liquiritin in licorice at the same time.国家自然科学基金2003重点项目(No.20235020

近海生态系统碳汇过程、调控机制及增汇模式

海洋是地球上最大的碳库,发挥着全球气候变化缓冲器的作用.蓝色碳汇,简称蓝碳,即由海洋生态系统捕获的碳(主要是有机碳),是海洋储碳的重要机制之一.; 蓝碳最初认识的形式是可见的海岸带植物固碳.其实之前没有得到足够重视的、看不见的微型生物(浮游植物、细菌、古菌、病毒、原生动物)占海洋生物量90%; 以上,是蓝碳的主要贡献者.中国陆架边缘海占国土总面积的1/3,碳汇潜力巨大,亟待研发.本文以近海生态系统碳汇过程、调控机制及增汇模式为主线,论述; 了近海生态系统结构与碳循环功能特征、碳汇形成过程与机理,并结合近海碳汇在沉积记录中的地史过程演变探讨了自然过程和人类活动对碳汇的可能影响,展望了; 碳汇工程在增加近海海洋储碳能力方面的应用前景.国家重大科学研究计划项目; 国家重点研发计划项目; 国家自然科学基金项目; 国家海洋局全球变化与海气相互作用专项项

七条建议,提升中年人幸福感

10月10日是世界精神卫生日。中年人是社会的中坚力量,精神心理健康的重要性不言而喻。心理幸福感也称心理满意度,是衡量心理健康水平的重要标志。人到中年,面临一系列挑战,心理幸福感会受到多方面因素影响。在心理学上,幸福感可通过多个方面进行观察和衡量,包括心态平衡、成长进步、目标价值、人际适应、家庭氛围、身心健康、自我接受、社会适应等。相关调查显示,中年人在以上各方面的体验均有提升空间。那么,中年人如何才能切实提升心理幸福感?本刊邀请精神心理领域专家结合中年人生活实际,从不同角度进行剖析、提出建议。</p