Experimental Investigation on the Early Stage Spray Characteristics with Biodiesel and Diesel

[EN] The early stage spray characteristics have a great impact on the secondary atomization progress, and thus affect the engine combustion and emission performances. The experimental investigation of the early stage spray behaviors with biodiesel and diesel was carried out by employing a laser-based Mie-scattering method. The results show that the spray tip penetration for biodiesel is higher than that for diesel at the early stage spray under the same injection pressure. Moreover, the early stage spray tip penetration can be longer under high injection pressures for two fuels. Besides, the early stage spray cone angle for biodiesel is narrower than that for diesel, and the spray cone angle is especially higher than biodiesel by 25.8% after start of injection time of 0.01ms. Furthermore, under the same injection condition, the difference of early stage spray area between diesel and biodiesel is not obvious, while the spray volume for biodiesel is larger than that for diesel, and also the spray volume can be enlarged by increasing injection pressure for both fuels.This work was supported by the contribution of China postdoctoral fund projects [grant number2013M530236]; The projects of ‘Six talent peak’ [grant number 2014-ZBZZ-014]; Research start-up found projects of Jiangsu university [grant number 13JDG104]; Natural Science Foundation of Jiangsu Province of China [grant number BK20150520]；The Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD]Yu, S.; Yin, B.; Wen, S.; Li, X.; Jia, H.; Yu, J. (2017). Experimental Investigation on the Early Stage Spray Characteristics with Biodiesel and Diesel. En Ilass Europe. 28th european conference on Liquid Atomization and Spray Systems. Editorial Universitat Politècnica de València. 473-479. https://doi.org/10.4995/ILASS2017.2017.4651OCS47347

Critical Roles of microRNA-141-3p and CHD8 in Hypoxia/Reoxygenation-Induced Cardiomyocyte Apoptosis

Background: Cardiovascular diseases are currently the leading cause of death in humans. The high mortality of cardiac diseases is associated with myocardial ischemia and reperfusion (I/R). Recent studies have reported that microRNAs (miRNAs) play important roles in cell apoptosis. However, it is not known yet whether miR-141-3p contributes to the regulation of cardiomyocyte apoptosis. It has been well established that in vitro hypoxia/reoxygenation (H/R) model can follow in vivo myocardial I/R injury. This study aimed to investigate the effects of miR-141-3p and CHD8 on cardiomyocyte apoptosis following H/R. Results: We found that H/R remarkably reduces the expression of miR-141-3p but enhances CHD8 expression both in mRNA and protein in H9c2 cardiomyocytes. We also found either overexpression of miR-141-3p by transfection of miR-141-3p mimics or inhibition of CHD8 by transfection of small interfering RNA (siRNA) significantly decrease cardiomyocyte apoptosis induced by H/R. Moreover, miR-141-3p interacts with CHD8. Furthermore, miR-141-3p and CHD8 reduce the expression of p21. Conclusion: MiR-141-3p and CHD8 play critical roles in cardiomyocyte apoptosis induced by H/R. These studies suggest that miR-141-3p and CHD8 mediated cardiomyocyte apoptosis may offer a novel therapeutic strategy against myocardial I/R injury-induced cardiovascular diseases

Experimental Analysis of Grease Friction Properties on Sliding Textured Surfaces

There is comprehensive work on the tribological properties and lubrication mechanisms of oil lubricant used on textured surfaces, however the use of grease lubrication on textured surfaces is rather new. This research article presents an experimental study of the frictional behaviours of grease lubricated sliding contact under mixed lubrication conditions. The influences of surface texture parameters on the frictional properties were investigated using a disc-on-ring tribometer. The results showed that the friction coefficient is largely dependent on texture parameters, with higher and lower texture density resulting in a higher friction coefficient at a fixed texture depth. The sample with texture density of 15% and texture depth of 19 μm exhibited the best friction properties in all experimental conditions because it can store more grease and trap wear debris. The reduction of friction is mainly attributable to the formation of a stable grease lubrication film composed of oil film, transfer film and deposited film, and the hydrodynamic pressure effect of the surface texture, which increases the mating gap and reduces the probability of asperity contact. This result will help in understanding the tribological behaviour of grease on a textured surface and in predicting the lubrication conditions of sliding bearings for better operation in any machinery

Factors driving the implementation of the ‘Local New Year’ policy to prevent COVID-19 in China

Abstract This study examines the contradiction caused by the ‘local new year’ policy, that is, the conflict between the pandemic prevention policies and people’s emotional demands during the Spring Festival, based on the normalisation of pandemic prevention and control. It focuses on the scientific logical relationship with the contradiction that people voluntarily support ‘local new year’, to explore the primary driving factors of their willingness. By evaluating the migrant workers in large cities, the primary influencing factors were screened, and the primary dynamic factors and their relationship were obtained using the Logit logical selection model and maximum-likelihood estimation. The study identified, ‘whether social and entertainment activities are planned in migrant cities’, as the primary driving factor, followed by ‘whether there are relatives (elderly /children) at home’, and ‘contracting the infection during travel’. In view of this conclusion, this study further proposes corresponding policy suggestions: Relevant measures should be adopted according to different regions and the current situation of the pandemic in combination with the characteristics of the episodic and local nature of the pandemic. ‘Local new year’ is encouraged from the perspective of enriching people’s emotional needs for spiritual entertainment and care. This study provides a new perspective and theoretical basis for the research and formulation of policies related to the normalisation of pandemic prevention and control in China and worldwide, and has a certain practical reference value

Critical role of Tim-3 mediated autophagy in chronic stress induced immunosuppression

Abstract Background Psychological and physical stress can either enhance or suppress immune functions depending on a variety of factors such as duration and severity of stressful situation. Chronic stress exerts a significantly suppressive effect on immune functions. However, the mechanisms responsible for this phenomenon remain to be elucidated. Autophagy plays an essential role in modulating cellular homeostasis and immune responses. However, it is not known yet whether autophagy contributes to chronic stress-induced immunosuppression. T cell immunoglobulin and mucin domain 3 (Tim-3) has shown immune-suppressive effects and obviously positive regulation on cell apoptosis. Tim-3 combines with Tim-3 ligand galectin-9 to modulate apoptosis. However, its impact on autophagy and chronic stress-induced immunosuppression is not yet identified. Results We found remarkably higher autophagy level in the spleens of mice that were subjected to chronic restraint stress compared with the control group. We also found that inhibition of autophagy by the autophagy inhibitor 3-methyladenine (3-MA) significantly attenuated chronic stress-induced alterations of pro-inflammatory and anti-inflammatory cytokine levels. We further elucidated that 3-MA dramatically inhibited the reduction of lymphocyte numbers. Moreover, chronic stress dramatically enhanced the expression of Tim-3 and galectin-9. Inhibition of Tim-3 by small interfering RNA against Tim-3 significantly decreased the level of autophagy and immune suppression in isolated primary splenocytes from stressed mice. In addition, α-lactose, a blocker for the interaction of Tim-3 and galectin-9, also decreased the autophagy level and immune suppression. Conclusion Chronic stress induces autophagy, resulting with suppression of immune system. Tim-3 and galectin-9 play a crucial regulatory role in chronic stress-induced autophagy. These studies suggest that Tim-3 mediated autophagy may offer a novel therapeutic strategy against the deleterious effects of chronic stress on the immune system

Effect of textured DLC coatings on tribological properties of titanium alloy under grease lubrication

To improve the tribological properties of titanium alloy surface and promote the functional application of titanium alloy, the synergistic anti-friction and wear-resistant effect of laser micro-textures and diamond-like carbon (DLC) coatings on titanium alloy surface under grease lubrication were investigated in this paper. Micro-textures and DLC coatings were fabricated on the surface of titanium alloy by Nd:YAG laser and magnetron sputtering technology. Effects of different surface treatment methods and micro-textures parameters on tribological properties of titanium alloy samples was studied. The results showed that, compared with the smooth titanium alloy sample, the friction coefficient of micro-textured sample, DLC coated sample and textured DLC coated sample decreased by 43.7%, 75.8%, 80.6% respectively. The surface of textured coated titanium alloy had the best tribological properties with a friction coefficient of 0.0799. The wear on the surface of the titanium alloy sample treated by laser micro-textures and DLC coatings was obviously improved. Compared with the severe adhesive wear on the surface of the titanium alloy sample, the surface of the textured coated titanium alloy sample was slightly scratched, and the wear volume was decreased by 97.5%. Meanwhile, DLC coatings with 30% textures density and 20 μ m textures depth showed the best anti-friction effect as well as the optimal hydrodynamic lubrication effect. Under the condition of grease lubrication, the surface of textured DLC coated titanium alloy substrate presented favorable anti-friction and wear-resistance effects, which revealed that reasonable micro-textures density and depth could more effectively exert the lubricating and anti-friction properties of textured DLC coatings

Bnip3 Mediates Doxorubicin-Induced Cardiomyocyte Pyroptosis via Caspase-3/GSDME

Aims: This study was aimed to investigate the role of GSDME-mediated pyroptosis in cardiac injury induced by Doxorubicin (DOX), and to evaluate the role of BH3-only protein Bcl-2/adenovirus E1B 19-kDa-interacting protein 3 (Bnip3) in regulation of DOX-induced pyroptosis. Main methods: HL-1 cardiomyocytes and C57BL/6J mice were treated by DOX to establish DOX-induced cardiotoxicity in vitro and in vivo models, respectively. Cell transfection was applied to regulate the expression of caspase-3, GSDME and Bnip3. Western blot was used for measuring expression of protein level. LDH-cytotoxicity assay was used to detect the LDH release. The Flow cytometry analysis was used to detect the cell death. Echocardiography was used to determine the cardiac function. HE staining was used for observing pathological feature of heart tissues. Key findings: Our results showed that GSDME-mediated pyroptosis was involved in DOX-induced cardiotoxicity in vivo. We showed that HL-1 cardiomyocytes exposed to DOX exhibited morphological features of pyroptosis in vitro. We also showed that DOX induced activation of caspase-3 and eventually triggered GSDME-dependent pyroptosis, which was reduced by the silence or inhibitor of caspase-3. We further showed that knockdown of GSDME inhibited DOX-induced cardiomyocyte pyroptosis in vitro. Finally, DOX increased the expression of Bnip3, whereas silencing of Bnip3 blunted cardiomyocyte pyroptosis induced by DOX, which was regulated through caspase-3 activation and GSDME cleavage. Significance: Our findings revealed a novel pathway that cardiomyocyte pyroptosis is regulated through Bnip3-caspase-3-GSDME pathway following DOX treatment, suggesting that Bnip3-dependent pyroptosis may offer a novel therapeutic strategy to reduce cardiotoxicity induced by DOX