利用real-time RT-PCR研究大型蚤对铜绿微囊藻毒素合成基因转录水平影响

近年来关于浮游动物与微囊藻相互作用的研究逐渐被关注,其中有的研究认为浮游动物能够诱导产毒细胞毒素含量的变化.微囊藻毒素是由微囊藻毒素合成基因编码翻译的,目前关于浮游动物对微囊藻毒素合成基因相对表达的影响并无报道,本文首次通过实时定量逆转录PCR方法研究铜绿微囊藻PCC7806产毒相关基因mcyB和mcyD在大型蚤胁迫下相对表达变化.结果显示微囊藻mcyB、mcyD基因相对表达均有上调,表明铜绿微囊藻PCC7806通过上调产毒基因的转录水平达到对大型蚤的诱导防御,从而为浮游动物与微囊藻相互作用研究提供新的依据

基于GDS-15评价老年卒中后抑郁状态的影响因素及其纵向分析（Influencing Factors and Longitudinal Analysis of Post-Stroke Depression in the Elderly Based on GDS-15）

目的 基于简版老年抑郁量表（geriatric depression scale 15，GDS-15）评估老年卒中后抑郁（post-stroke depression，PSD）的影响因素，并纵向分析其抑郁状态的变化情况。 方法 回顾性分析2019年1月—2021年12月合肥市第三人民医院收治的老年首发急性缺血性卒中（acute ischemic stroke，AIS）患者的临床资料，记录患者出院时的GDS-15评分以评估PSD发生情况。将GDS-15评分＞10分的患者纳入PSD组，≤10分的患者纳入非PSD组。比较两组临床资料，使用多因素logistic回归分析评估老年AIS患者发生PSD的危险因素，并对出院后随访时间≥12个月患者的GDS-15评分进行纵向分析。 结果 共纳入168例老年AIS患者，其中PSD组57例，非PSD组111例。PSD组大专及以上学历患者的占比低于非PSD组（P=0.042），入院时NIHSS评分＞4分（P=0.007）、入院时改良巴塞尔指数（modified Barthel index，mBI）评分＜60分（P=0.003）及入院时老年营养风险指数（geriatric nutritional risk index，GNRI）≤98（P=0.021）的占比高于非PSD组。多因素logistic回归分析显示，入院时NIHSS评分＞4分（OR 2.489，95%CI 0.017～0.995，P＜0.05）、入院时mBI评分＜60分（OR 3.357，95%CI 1.883～5.986，P＜0.05）及入院时GNRI≤98（OR 2.818，95%CI 1.537～5.165，P＜0.05）是老年AIS患者发生PSD的危险因素，而大专及以上学历是老年AIS患者发生PSD的保护因素（OR 0.519，95%CI 0.017～0.995，P＜0.05）。168例老年AIS患者中，有69例出院后随访时间≥12个月，出院后12个月GDS-15评分、mBI评分及GNRI高于出院时（P均＜0.01），而NIHSS评分低于出院时（P＜0.01）。 结论 老年AIS患者出院后抑郁水平较高，入院时神经功能损伤严重、日常活动能力受限明显及营养状态不佳的患者，更易发生PSD；而大专及以上学历的老年AIS患者PSD发生风险较低。Abstract: Objective To evaluate the influencing factors of post-stroke depression (PSD) in the elderly based on the geriatric depression scale 15 (GDS-15) and to longitudinally analyze the changes in depressive status. Methods A retrospective analysis was performed on the clinical data of elderly patients with first-episode acute ischemic stroke (AIS) admitted to the Third People’s Hospital of Hefei from January 2019 to December 2021. All patients completed the GDS-15 questionnaire at discharge to evaluate the occurrence of PSD. Patients with a GDS-15 score>10 were included in the PSD group, while those with a score≤10 were included in the non-PSD group. Clinical data were compared between the two groups, and multivariate logistic regression analysis was used to assess the risk factors for PSD in elderly patients with AIS. Longitudinal analysis of GDS-15 scores was performed for patients with a follow-up period of ≥12 months after discharge. Results A total of 168 elderly AIS patients were enrolled, including 57 patients in the PSD group and 111 patients in the non-PSD group. The proportion of patients with an education level of junior college or above in the PSD group was lower than that in the non-PSD group (P=0.042). The proportions of patients with an admission NIHSS score>4 points (P=0.007), an admission modified Barthel index (MBI) score4 points (OR 2.489, 95%CI 0.017～0.995, P<0.05), an admission MBI score<60 points (OR 3.357, 95%CI 1.883～5.986, P<0.05), and an admission GNRI≤98 (OR 2.818, 95%CI 1.537～5.165, P<0.05) were risk factors for PSD in elderly patients with AIS, while an education level of junior college or above was a protective factor (OR 0.519, 95%CI 0.017～0.995, P<0.05). Among the 168 elderly patients with AIS, 69 patients had a follow-up period of≥12 months after discharge. At 12 months after discharge, GDS-15 score, MBI score, and GNRI were higher than those at discharge (P all<0.01), while NIHSS score was lower than that at discharge (P<0.01). Conclusions Depressive levels in elderly patients with AIS may increase after discharge. Those with severe neurological impairment, significant limitations in activities of daily living, and poor nutritional status at admission are more likely to develop PSD, whereas those with an education level of junior college or above have a low risk of PSD

25MeV／u~（40）Ar＋~（159）Tb反应中等质量碎片的热核蒸发

在２０°至１５５°范围内测量了２５ＭｅＶ／ｕ的４０Ａｒ轰击１５９Ｔｂ靶产生的单举中等质量碎片（３≤Ｚ≤９）能谱．后角区中等质量碎片能谱具有明显的统计蒸发特性，不同元素的能谱可用相同参数的单个运动源模型很好地拟合．拟合提取的源速度和核温度等源参数与非完全熔合所形成热核的反冲速度和核温度近似一致，表明这些不同的中等质量碎片共同起源于热核的统计蒸发．使用统计程序ＧＥＭＩＮＩ计算了碎片角分布和电荷分布．The inclusive energy spectra of intermediate-mass fragments(IMF)(3≤Z≤9)have been measured in the range from 20°to 155°for 40Ar+159Tb reaction at 25MeV/u.At backward angles,the spectra of IMF show a characteristic of statistic evaporation and can be well fitted by a moving source with same parameters.The obtained source velocity and temperature are in agreement with the corresponding values of hot nuclei produced in an incomplete-fusion process.Therefore,it seems that there IMF are statistically evaporated f...国家科委基础研究,国家自然科学基

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^