Portfolio: SONG Yong-Hong: Burning Realities 宋永红：灼热的现实

This Portfolio features 1) numerous essays on the artist (in both Chinese and English); 2) images of the artist\u27s selected works (with captions); and 3) a brief bibliographical account of the artist. (Jerry Wu\u2723).https://digital.kenyon.edu/zhoudocs/1242/thumbnail.jp

Electrochemical oxidation of isopropanol on Pt microdisc electrode

采用微电极伏安技术，研究异丙醇在PT微盘电极（rd＝10μM）上氧化的稳态和暂态的电化学行为，得出一系列重要的动力学参数：异丙醇氧化反应的反应电子数n为1.95，扩散系数d为3.22x10-5CM2/S，传递系数β值为0.12，电极体系的交换电流密度J0为3.0x10-4A/CM2，标准速度常数kS为1.60x10-4CM/S。由计算结果我们可以推测，异丙醇在PT微盘电极上酸性水溶液体系中的氧化过程由稳态传质扩散及电化学极化联合控制，其中，异丙醇分子到电极表面的扩散吸附为快速步骤，吸附的异丙醇分子在电极表面脱氢为慢步骤；异丙醇在PT微盘电极上的电化学氧化为异丙醇脱氢生成丙酮的2电子转移反应，并由此推测其可能的反应途径。The　stable　and　transient　electrochemical　behavior　of　isopropanol　oxidation　on　Pt　microdisc　electrode(rd=10μm)　were　investigated　by　voltammetric　microelectrode　technique.A　series　of　an　important　dynamic　parameters　were　obtained:n=1.95,D=3.22×10-5cm2/s,β=0.12,J0=3.0×10-4A/cm2,Ks=1.60×10-4cm/s(n——number　of　electrons　involved　in　oxidation　process;D——diffusion　coefficient;β——transfer　coefficient;J0——exchange　current　density;Ks——standard　rate　constant).From　the　results,it　can　be　considered　that　the　processes　of　isopropanol　oxidation　on　Pt　microdisc　electrode　in　the　acidic　aqueous　solution　were　simultaneously　controlled　by　steady　state　mass　transfer　diffusion　and　electrochemical　polarization.In　the　case　of　the　processes　of　isopropanol　diffuses　and　absorbs　to　the　surface　of　electrode　are　fast　step,and　the　processes　of　the　absorbed　isopropanol　dehydrogenate　on　the　surface　of　the　electrode　are　slow　step.The　results　show　that　the　main　oxidation　reaction　is　a　two　electrons　transfer　reaction　of　isopropanol　hydrogenation　to　acetone.The　probable　electrochemical　oxidation　mechanism　were　proposed

The Full Length cDNA Cloning and Expression Analysis of Catalase from the Kuruma Shrimp(Marsupenaeus japonicus)

采用反转录PCr(rEVErSE TrAnSCrIPTIOn PCr,rT-PCr)及CdnA末端快速扩增(rAPId-AMPlIfICATIOn Of CdnA EndS,rACE)技术,在日本囊对虾(MArSuPEnAEuS JAPOnICuS)肝胰腺组织克隆到过氧化氢酶(CATAlASE,CAT)基因CdnA全序列.日本囊对虾CAT基因(MJCAT)CdnA全长1 802bP,开放阅读框(OPEn rEAdIng frAME,Orf)长1 563bP,编码520个氨基酸,理论等电点为7.26,分子质量为58.9ku.同源建模分析表明,MJCAT蛋白分子结构高度保守.同源性比对后发现,MJCAT基因氨基酸序列与中国明对虾(fEnnErOPEnAEuS CHInEnSIS)相似性最高(94.2%),其次是凡纳滨对虾(lITOPEnAEuS VAnnAMEI)(90.9%).随后的聚类分析表明,其与中国明对虾和凡纳滨对虾聚为一支.荧光定量结果显示,MJCAT基因在检测的多种组织中均有表达,其中以肝胰腺中表达量最高,其次为眼柄、肠、鳃、胃、心脏和血细胞,在肌肉中的表达水平最低.白斑综合征病毒(WHITE SPOT SyndrOME VIruS,WSSV)感染12H后,肝胰腺MJCAT基因的表达量逐渐升高,48H上调至最高的表达水平.研究表明,MJCAT基因在维持机体稳态及抗氧化防御反应过程中发挥着重要的作用.The catalase gene(MjCAT)from the hepat opancreas of Marsupenaeus japonicuswas cloned using reverse transcription PCR(RTPCR)and rapid-amplification of cDNA ends(RACE)methods in the present study.The full length cDNA of MjCATconsisted of 1 802bp with an open reading frame(ORF,1 563bp)encoded 520amino acid resides.The molecular mass and isoelectric point of MjCAT protein were predicted to be 58.9ku and 7.26respectively.Homology modeling revealed that the potential spatial structure of MjCAT protein was highly conserved.By homologous analysis,the amino acid sequence of MjCAT had the highest similarity(94.2%)with that fromFenneropenaeus chinensis,and followed by Litopenaeus vannamei(90.9%).Then phylogenetic analysis indicated that its was first clustered together with F.chinensis and L.vannamei.Real-time quantitative PCR results displayed that MjCATtranscripts could be detected in all the tested tissues,with the highest expression level in the hepatopancreas,then in eyestalk,intestine,gills,stomach,heart and hemocytes,and the lowest in muscle.After white spot syndrome virus(WSSV)infection,the expression level of MjCATin the hepatopancreas began to increase gradually after12hand reached apeak at 48h.These findings suggested that MjCAT was of pivotal importance for the maintenance of internal homeostasis and antioxidant defense in M.japonicus.国家高技术研究发展计划(863)项目(2012AA10A409); 国家虾产业技术体系建设项目(CARS-47

克氏原螯虾壳虾青素提取条件的优化

以新鲜克氏原螯虾壳为原料,比较了冷冻干燥、常温风干和烘干3种前处理方式对克氏原螯虾壳虾青素的提取效果。采用单因素试验研究了提取溶剂、料液比例、浸提温度、浸提时间、提取次数对虾青素提取效果的影响,用紫外分光光度法测定虾青素的含量。结果表明,丙酮的萃取效果优于无水乙醇和石油醚,风干和烘干处理的虾壳虾青素提取量显著低于冷冻干燥处理,最佳提取条件为：料液比1∶20（g∶mL）、温度30℃、时间2 h、重复提取2次。最佳提取条件下2次提取的加标回收率为99.2%,新鲜虾壳虾青素平均提取量为148.2μg/g

萘及其衍生物对普通小球藻的毒性效应

本文研究了荣及其衍生物（萘、1-萘酚、2-萘酚和1-萘胺）对普通小球藻的生长、叶绿素，含量、光合强度和呼吸强度的影响，萘、1-萘酚和1-萘胺在低浓度下能促进普通小球藻的生长，高浓度则抑制藻的生长；2-萘酚在实验浓度下都抑制藻的生长．萘、1-萘酚、2-萘酚和1-萘胺的96hEC50分别为98．06、11．87、13．39和6．95mg／L．萘及其衍生物对叶绿素含量和光合强度的影响比对生长的影响强，而对叶绿素含量的影响又比对光合强度的影响强．可以这样认为：萘及其衍生物对普通小球藻生长的抑制是通过抑制藻叶绿素

The nurse practice environment in primary health care

Orientador: Edinêis de Brito GuirardelloTese (doutorado) - Universidade Estadual de Campinas, Faculdade de EnfermagemResumo: Estudos em ambientes hospitalares apontam associações entre percepções de atributos do ambiente da prática profissional, síndrome de burnout e as variáveis: satisfação profissional, qualidade do cuidado, intenção de deixar o trabalho atual e intenção de deixar a enfermagem, destacando que esses achados podem contribuir na construção de indicadores de qualidade do serviço e gestão de recursos humanos. No entanto, não existem estudos dessa natureza, no Brasil, em atenção primária à saúde. Esta pesquisa teve por objetivo avaliar percepções do enfermeiro acerca do ambiente da sua prática na atenção primária à saúde de um município no interior do estado de São Paulo. Trata-se de estudo transversal. Para a coleta de dados foi utilizada uma ficha de caracterização pessoal e profissional acrescida das variáveis: satisfação no trabalho, percepção da qualidade do cuidado, intenção de deixar o trabalho atual e intenção de deixar a enfermagem; o Nursing Work Index Revised para analisar: autonomia, controle sobre o ambiente da prática, relação profissional cordial entre enfermeiro e médico e suporte organizacional e; o Inventário de Burnout de Maslach para analisar: exaustão emocional, despersonalização e realização pessoal. Participaram 198 enfermeiros. Os resultados evidenciaram médias entre 1,94 a 2,65 para as subescalas do Nursing Work Index Revised (variação de 1 a 4 pontos); 27,98% de enfermeiros com exaustão emocional alta; 32,14% com despersonalização alta; 38,69% com realização pessoal reduzida; 62,63% satisfeitos no trabalho; 34,85% insatisfeitos; 78,97% avaliaram a qualidade do cuidado como boa; 13,33% avaliaram-na como ruim; média da intenção de deixar o trabalho atual de 28,40/100 milímetros e média da intenção de deixar a profissão de 14,40/100 milímetros. Também foram evidenciadas 23 correlações significantes. O estudo sugere que o ambiente da prática profissional do enfermeiro em atenção primária à saúde é parcialmente favorável (1/3) para as percepções de: autonomia, relação profissional cordial entre enfermeiro e médico e suporte organizacional e parcialmente desfavorável (2/3) para controle sobre o ambiente da prática profissional; aproximadamente um terço dos enfermeiros apresentam nível alto de burnout e outro um terço, nível moderado. Percepções do enfermeiro acerca do ambiente da prática influenciam a ocorrência da síndrome de burnout, a satisfação no trabalho, a percepção da qualidade do cuidado e a intenção de deixar o trabalho atual, e sentimentos de burnout influenciam as mesmas variáveis e a intenção de deixar a enfermagem. Esses achados dão visibilidade à situação de trabalho do enfermeiro na atenção primária à saúde, contribuem para esclarecer a influência dos fatores organizacionais no trabalho e na construção de indicadores para avaliação de serviços de saúde, recursos humanos em enfermagem e saúde do trabalhador; apontam para a necessidade de avaliar o modelo da prática profissional do enfermeiro, o modelo de prestação de cuidados e a mensuração de resultados das dimensões qualitativas do trabalho do enfermeiro na atenção primária à saúde, fundamentados no conceito de vigilância em saúde e auxiliados pela estratégia da educação permanenteAbstract: Studies in hospital settings show associations among perceptions of the attributes of professional practice environment, burnout and the variables: job satisfaction, quality of care, intention to leave the current and intention to leave nursing work, noting that these findings can help construction of indicators of quality of service and human resource management. However, no studies of this nature in Brazil in primary health care. This research aimed to evaluate perceptions of nurses about their practice in primary health care in a municipality in the state of São Paulo environment. This cross-sectional study. To collect data characterizing a form of personal and professional plus variable was used: job satisfaction, perception of quality of care, intention to leave the current job and intention to leave nursing; the Nursing Work Index Revised to analyze: autonomy, control over practice environment, friendly professional relationship between nurse and physician and organizational support, and the Maslach Burnout Inventory to analyze: emotional exhaustion, depersonalization and personal accomplishment. Attended the survey 198 nurses. The results showed averages between 1.94 to 2.65 for the subscales of the Nursing Work Index Revised (ranging from 1 to 4 points); 27.98% of nurses with high emotional exhaustion; 32.14% with depersonalization; 38.69% with reduced personal accomplishment; 62.63% satisfied at work; 34.85% dissatisfied; 78.97% rated the quality of care as good; 13.33% rated it as bad; mean intention to leave the current job 28.40/100 mm and the intention to leave the profession of 14.40/100 mm average. Were also observed 23 significant correlations. The study suggests that the environment of professional nursing practice in primary health care is partially positive (1/3) to perceptions of: autonomy, cordial professional relationship between nurse and physician and organizational support and partially unfavorable (2/3) for control over the environment of professional practice; approximately one third of nurses have a high level of burnout, and another third, moderate level. Perceptions of nurses about the practice environment influence the occurrence of burnout, job satisfaction, perception of quality of care and intention to leave the current job, and feelings of burnout influence the same variables and intention to leave nursing. These findings provide visibility to the work situation of nurses in primary health care, help to clarify the influence of organizational factors at work and the construction of indicators for the evaluation of health services, nursing human resources and occupational health; point to the need to assess the model of professional nursing practice, the model of care and measurement of results of the qualitative dimensions of the work of nurses in primary health care, based on the concept of health surveillance and aided by education strategy permanentDoutoradoEnfermagem e TrabalhoDoutora em Enfermage

两种绿藻辅助饲喂对克氏原螯虾生理活性的影响

以克氏原螯虾为研究对象,测定了在正常投喂人工颗粒饲料的基础上辅助添加小球藻、刚毛藻饲喂克氏原螯虾的生理活性。结果表明:试验期间添加小球藻(1.0×10^6—1.0×10^7 cells·L^-1)、刚毛藻(0.06—0.08 g·L^-1)对克氏原螯虾的生长与存活率均无显著影响(P>0.05),但肌肉超氧化物歧化酶(SOD)和肝胰脏酸性磷酸酶(ACP)的活性显著降低(P<0.05);机体的血细胞密度显著增加(P<0.05),外骨骼和肌肉中虾青素的含量极显著增加(P<0.01);单独添加刚毛藻肌肉虾青素含量也显著提高(P<0.05),血清、肝胰脏和肌肉碱性磷酸酶(AKP)的活性极显著提高(P<0.01),肝胰脏酸性磷酸酶的活性极显著降低(P<0.01)。摄食小球藻和刚毛藻对于克氏原螯虾的抗逆活性和免疫能力具有促进作用,养殖水体中适度添加小球藻和刚毛藻有助于克氏原螯虾的健康与活力

三峡水库沉积物不同赋存形态磷的时空分布

为了认识三峡水库沉积物磷的赋存状况,利用磷形态标准测试程序SMT法对干流和三条代表性支流(香溪河、大宁河、小江)的柱状沉积物进行了总磷(TP)、无机磷(IP)、有机磷(OP)、铁/铝磷(Fe/Al-P)、钙磷(Ca-P)的测定,结果表明干流沉积物TP含量为781~1026 mg·kg-1,支流沉积物TP含量为382~1085 mg·kg-1.TP主要由IP组成,OP所占比例较低;IP主要由Ca-P组成,Fe/Al-P所占比例较低.干流TP含量空间差异不显著,但各赋存形态磷的含量普遍高于支流,支流中香溪河磷含量高于大宁河和小江.垂直方向上各赋存形态磷含量在不同沉积深度没有明显规律;TP、IP、Ca-P三者变化趋势较一致,主要受Ca-P含量的影响.鉴于支流的独特水文条件,相比于干流,更应警惕支流沉积物磷的释放风险及其对水体的环境化学效应