A preparation for the remediation of petroleum hydrocarbon pollutants and its application

本发明属于土壤修复技术领域，具体的说是涉及一种修复石油烃污染物的制剂及其应用。制剂为微生物固定于裂解获得的生物炭材料中；其中，炭材料中微生物的单载量为3.2‑6.5×1010cfu/g；所述微生物为不动杆菌HC8‑3S和迪次氏菌CN‑3。本发明复配的表面活性剂清洗液清洗效率高，土壤残留小。制备的生物炭固定化微生物材料具有固定率高、生产成本低、促进土壤微生态环境改良、对环境不造成二次污染等优点，用于石油及石油产品污染土壤的修复

Rhizosphere microbial community structure and driving mechanism of Zostera marina and Zostera japonica in Swan Lake, Weihai

【Objective】 This research aims to investigate the diversity of microbial community structure in different seagrass growth periods, and to analyze the internal factors that lead to the differences in microbial community structure. [Methods] The rhizosphere sediments of seagrass and unvegetated areas surface sediments were collected. High throughput sequencing (Illumina MiSeq Platform) was used to analyze the microbial community characteristics of rhizosphere and unvegetated areas at different seagrass growth stages. [Results] The difference in microbial community structure was driven by the growth period of seagrass and whether seagrass was colonized. At the maturation stage of seagrass, Propionium was significantly enriched in the roots of Z. marina and Z. japonica, with a relative abundance of 11.58% and 14.26%, respectively. In the seedling stage of seagrass, Desulfobulbaceae was enriched at the seagrass roots (Z. marina: 2.299%, Z. japonica: 4.092%). The relative abundance of Sulfurovum was higher in the rhizosphere during the decline stage of seagrass (Z. marina: 5.624%, Z. japonica: 3.749%). In addition, the growth period of seagrass had the greatest explanatory power for the differences in microbial community structure (R~2=0.20335,P=0.002). The results of PICRUSt2 function prediction showed that all functional genes showed the same trend in different growth stages of seagrass, but the abundance showed the result of seedling stage>mature stage>decline stage. [Conclusion] The microbial community structure of seagrass bed sediments in Swan Lake presented different diversity characteristics at different growth stages of seagrass, with obvious rhizosphere effect and no significant difference in the rhizosphere microbial community of different species of seagrass, which was not species-specific

Rhizosphere microbial community structure and function of Zostera japonica in the distribution area of seagrass beds in the Yellow Sea and Bohai Sea

Zostera japonica is a unique Asian seaweed species and has important ecological value. In recent years, Zostera japonica has been continuing to degenerate, causing widespread concern among researchers. [Objective] We hypothesized that the rhizospheric microorganisms were closely related with the healthy growth of Zostera japonica. We explored the diversity and function of microbial community in root of Zostera japonica at coastal zone of Yellow sea and Bohai sea, and intrinsic relationship with seagrass. [Methods] We collected surface sediment samples of Zostera japonica roots and unvegetated areas from three locations including Dongying, Weihai, and Dalian city. We investigated the rhizosphere microbial community structure by sequencing on Illumina HiSeq300 platform and analyzed the correlation between microbial community structure and environmental parameters. [Results] The most dominated phyla widely distributed in the rhizospheric surface sediments of Zostera japonica included: Proteobacteria (41.1%), Cyanobacteria (15.4%), Bacteroidetes (12.6%), Actinobacteria (9.3%). Comparisons of different regions (Weihai, Dongying, and Dalian) or sample types (rhizosphere vs. non-rhizosphere) showed significant differences in microbial communities, mainly due to the presence of sulfate-reducing bacteria and nitrogen-fixing bacteria found in the rhizosphere. Total nitrogen, total carbon, total organic carbon, clay, were all significantly correlated to rhizospheric bacterial community composition and distribution. [Conclusion] From a functional point of view, the differences between groups was mostly related to sulfur and nitrogen cycling, and sulfate reducing bacteria play a key role in maintaining the ecological health of Zostera japonica.</p