Construction of the Drosophila with overexpression of kinase domain of hEphB4 and its application in drug screen

hEphB4是体内最大的受体酪氨酸激酶家族hEph中的一员，它在体内正常情况下，与它的配体ephrin一起，参与胚胎期血管生成和血管再生。它的缺失会造成老鼠在胚胎期死亡。近几年的研究证明，hEphB4的过表达与肿瘤的发展有密切的关系。它参与到肿瘤血管的生成，促进了肿瘤组织获得营养。它的激酶结构域参与了相关信号通路的转导，导致细胞的无限增殖。因此，很多研究都在尝试去得到hEphB4的激酶抑制剂。在这方面，已经取得了很多进展，但是目前所得到的抑制剂存在一个共同的问题。对激酶活性的抑制是有效的，但是特异性很差，同时抑制了其他激酶的活性。 为了获得特异性更高的hEphB4的激酶抑制剂，我们将建立一个...HEphB4 , a member of Eph receptors which are the largest family of receptor tyrosine kinases. HEphB4 and its ligand Ephrin-B2 is nessesary in the maturation of the vessel of the embro and the regeneration of new vessels. Loss of hEphB4 cause the death of mouse in the embryo.Recent years it is reported that up-regulation of hEphB4 is correlated to the progression of the tumor.In fact hEphB4 play t...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：2172009115217

Characterization of Coenzyme Q and Preliminary Investigation on Its Effect on Life-span in Drosophila melanogaster

辅酶Q是存在于线粒体内膜上的脂溶性电子传递体,它是呼吸链的重要组成部分.辅酶Q在不同生物体内其侧链异戊二烯单位的数目不同,目前对黑腹果蝇(drOSOPHIlA MElAnOgASTEr)中辅酶Q的存在形式和含量还不清楚.利用高效液相色谱法分析鉴定了模式生物黑腹果蝇中辅酶Q的种类及含量.实验结果表明:黑腹果蝇中辅酶Q9与Q10并存,辅酶Q9的含量约为Q10的2.5倍.辅酶Q含量具性别差异,其中辅酶Q9在雌性黑腹果蝇中含量较雄性约高30%,而辅酶Q10在雌性果蝇中则比雄性果蝇高约33%;辅酶Q9与Q10含量在辅酶Q合成酶COQ2的突变体中明显降低并且COQ2突变体的寿命较野生型略短.这些实验结果将为利用黑腹果蝇模型研究辅酶Q的功能提供重要基础.Coenzyme Q is an essential component of the mitochondrial respiratory chain as a lipid-soluble electron transporter located in the inner membrane of mitochondria.The number of isoprenoid subunits in the coenzyme Q side chain varies in different species.The content of coenzyme Q in Drosophila has not been reported yet.In the present report,we analyzed the content of coenzyme Q in adult Drosophila by HPLC.We provided evidence that Drosophila contains both CoQ9 and CoQ10.The amount of CoQ9 is about 2.5 folds than that of CoQ10.We further showed the content of CoQ9 and CoQ10 in females are respectively about 30%,33% higher than that in males.In addition,both concentrations of CoQ9 and CoQ10 are significantly reduced in the mutant of CoQ synthase gene coq2 which appeared with shortened life-span.These results provided a good basis to further investigate in vivo biological functions of CoQ in Drosophila.国际科技合作项目(2009DFA32760