Research on The Development Strategy of O2O Family Hotel Based on Customer Satisfaction Survey ——A Case of Family Hotel in Xiamen

近年来，“互联网+”的模式遍地开花，各行各业都在尽可能地将自身的业务向互联网行业靠拢，旅游业也不例外。在法定节假日的助推下，顾客对于旅游投资规模不断扩大，各地通过旅游经济来改善当地民生和拉动经济增长成效显著，住宿行业也得到如火如荼的发展。随着O2O模式爆发式增长，与旅游业、住宿行业密切相关的家庭旅馆行业发展也顺利搭上O2O的顺风车，成为主要受益者之一。 全文共分为六个部分。第一章阐述选题背景及意义、简要介绍研究目的、内容和框架；第二章介绍O2O模式、顾客满意度等相关概念，结合家庭旅馆行业发展情况，论述互联网与家庭旅馆之间的关系；第三章研究厦门旅游业、住宿行业、家庭旅馆行业的发展现状，指出厦门...In recent years, the mode of " Internet plus" blossom everywhere, in all walks of life as much as possible in their own business to move closer to the Internet industry, the tourism industry is no exception. In the boost of staturoy holiday, the customer for the scale of tourism investment continues to expand, all over through the tourism economy to improve the local people's livelihood and pullin...学位：工商管理硕士院系专业：管理学院_工商管理硕士(工商管理硕士)学号：1792010115087

The Research on Mesoporous Alumina-Supported Nickel Oxides Catalysts for Oxidative Dehydrogenation of Propane to Propene:

低碳烷烃（C1-C4）的选择氧化是当今催化研究领域面临的巨大挑战之一。而在其中，丙烷氧化脱氢制丙烯（ODHP）是一个具有重大研究价值与工业应用前景的反应。到目前为止，应用于该反应且有较好性能的催化剂主要以钒基、钼基催化剂为主。但该类催化剂所需的反应温度较高，一般在550oC以上。由于该反应的产物丙烯较反应物丙烷更为活泼，因此前者在反应条件下极易深度氧化，使反应产物丙烯的选择性降低。因此，降低所需反应温度，合成制备具有高选择性高活性的低温催化剂具有重要的意义。针对上述反应，本论文制备了一系列氧化铝负载氧化镍催化剂，系统考察了制备方法对催化剂结构、表面性质以及催化性能的影响，研究了催化剂的构效关系...The selective oxidation of low carbon alkane is one of the greatest challenges of the present catalysis research, in which the oxidative dehydrogenation of propane (ODHP) is fairly crucial because of its promising industrial potential. Until now, the vanadium- and molybdenum-based catalysts have been regarded as the main and better catalysts for this reaction. But the reaction temperature are usua...学位：理学硕士院系专业：化学化工学院化学系_物理化学(含化学物理)学号：2052009115139

A Study on Service Marketing Strategies of China Public Library

公共图书馆是各类型图书馆中最为重要的一种类型，是民主社会保障公民平等地获取知识和信息的社会机构。但自上个世纪80年代以来，我国公共图书馆界遭遇的最大困境之一却是经费紧缺，由此引发了很多问题。图书馆界不少人士进行分析，提出多种可能原因并提出一些应对措施。国家不重视公共图书馆事业可能是几种原因合力的结果，但是对公共图书馆来说能做的只有从自身找原因。 从我国公共图书馆的使命和公共图书馆服务的根本属性看，公共图书馆属于公共服务业。在公共图书馆工作中应用服务营销的理论和方法是必然和必要的，并且是提高自身服务水平和改善服务质量、摆脱困境的途径之一；也是公共图书馆发展核心竞争力以应对当今瞬息万变的环境、以...The public library which is the most important types of various libraries is a social organization to guarantee the citizen to obtain knowledge and information equally in democratic society. Since 1980's, the main predicament of the public library is funding shortage, which induced many problems. The people in library circle analyzed the problem and proposed some measures .Country do not attach im...学位：管理学硕士院系专业：管理学院企业管理系_企业管理(含财务管理、市场营销、人力资源管理)学号：K20051412

Programmed cryopreservation of the amphioxus Branchiotoma belcheri embryos

文昌鱼在进化和发育生物学中有重要科学研究价值,然而由于文昌鱼实验室繁殖受产卵季节等因素的限制,以其胚胎为材料的研究工作受到局限,胚胎冷冻保存是解决这一问题的有效途径之一。本文首次对文昌鱼(Branchiostoma japonicum)胚胎的程序化冷冻保存进行了研究,通过筛选合适的程序化抗冻液、适宜的胚胎发育阶段,以及比较不同平衡处理时间、植冰温度、保存温度和洗脱时间对文昌鱼胚胎冷冻保存的影响。结果表明:几种抗冻液中,A2的效果较好,对胚胎的毒性小;原肠中期胚胎较其它时期对抗冻液的耐受力更强,适宜进行冷冻保存;胚胎在抗冻液中的最佳平衡处理时间为40-50min;冷冻降温时,适宜的植冰温度为-7.0℃--10.0℃;植冰后进行程序化冷冻,目前能稳定得到存活胚胎的最低保存温度为-15℃;此外,不同的洗脱时间对胚胎的存活率无显著影响。根据优化后的条件,对文昌鱼胚胎进行程序化冷冻保存(-196℃)实验,获得1粒存活的胚胎,并孵化出膜,发育至两鳃裂时期,幼鱼共存活了8d,但形态畸形。结果提示文昌鱼胚胎冷冻保存的可行性,但需要进一步优化包括抗冻液在内的各种条件,以提高存活率。In the present study, we investigated the possibility of cryopreservation of amphioxus Branchiostoma japonicum embryos. We found that programmed freezing solution A1 was good for the cryopreservation of amphioxus embryos. The test of toxicity tolerance of embryos at various developmental stages towards the solution A1 showed that embryos at the metaphase of gastrulation were more tolerant of the cryoprotectant solution than embryos at other stages. The optimal equilibration time of embryos treated with A1 was 40-50 min. We also compared the effects of seeding temperature, cryopreservation temperature and elution time during the process. The survival rates after seeding at -7℃ and -10℃ were 46% and 42% respectively, but no embryo survived after seeding at -12℃. Some embryos could survive preserved at -15℃, but all embryos perished at lower temperatures. When elution time varied, the survival rate of amphioxus embryos did not vary significantly. Similar to fish, the membrane permeability of amphioxus embryos to water and cryoprotectants is low, which is probably a major obstacle to successful cryopreservation, and causes lethal intracellular ice formation. We used A1 as a programmed freezing solution to cryopreserve different embryonic stages, and stored them in liquid nitrogen (-196℃). Of those embryos, one was successfully recovered and developed to the two-gill-slit stage. Eventually, it died due to morphological abnormality after surviving for eight days. These results indicate the possibility of cryopreservation methods for amphioxus embryos.国家自然科学基金(No.30470938和No.30570208);; 福建省自然科学基金(No.D0510002);; 厦门市科技局科技计划重点项目(No.3502Z20042015)资助~

Comparative Analysis of Complete Mitochondrial DNA Control Region of Four Species of Strigiformes

利用Long-PCR和PrimerWalking的方法对鸮形目的短耳鸮、长耳鸮、纵纹腹小鸮、灰林鸮4种鸟类的线粒体调控区进行了全序列测定。结果表明:短耳鸮的调控区长度为3290bp;长耳鸮为2848bp;纵纹腹小鸮为2444bp;灰林鸮为1771bp。短耳鸮的调控区长度是4种鸮中最大的,并且是目前已知最大的鸟类线粒体调控区。这4种鸮类调控区的基本结构和其他鸟类相似,按照碱基变化速率的不同可以分为3个区:碱基变化速率较快的外围区域Ⅰ、Ⅲ和保守的中间区域Ⅱ。这4种鸟类调控区的3′端均存在大量的串联重复序列,短耳鸮为126bp单元重复7次和78bp单元重复14次;长耳鸮为127bp单元重复8次和78bp单元重复6次;纵纹腹小鸮有3个重复单元,分别为89bp单元重复3次、77bp单元重复4次和71bp单元重复6次;灰林鸮仅有1个单元的串联重复为78bp重复5次。调控区中串联重复序列可能是由链的滑动错配产生,另外这些重复序列都能形成热力学稳定的多重茎环二级结构,而且在重复序列中还发现一些保守基序,这说明重复序列可能具有一定的生理功能,影响调控区的调控功能从而影响线粒体基因组的复制和转录。The sequence of the whole mitochondrial (mt) DNA control region (CR) of four species of Strigiformes was obtained. Length of the CR was 3 290 bp, 2 848 bp, 2 444 bp, and 1 771 bp for Asio flammeus, Asio otus, Athene noctua, and Strix aluco, respectively. Interestingly, the length of the control region was maximum in Asio flammeus among all the avian mtDNA control regions sequenced thus far. In addition, the base composition and organization of mtDNA CR of Asio flammeus were identical to those reported for other birds. On the basis of the differential frequencies of base substitutions, the CR may be divided two variable domains, Ⅰ and Ⅲ, and a central conserved domain, Ⅱ. The 3′ end of the CR contained many tandem repeats of varying lengths and repeat numbers. In Asio flammeus, the repeated sequences consisted of a 126 bp sequence that was repeated seven times and a 78 bp sequence that was repeated 14 times. In Asio otus, there were also two repeated sequences, namely a 127 bp sequence that was repeated eight times and a 78 bp sequence that was repeated six times. The control region of Athene noctua contained three sets of repeats: a 89 bp sequence that was repeated three times, a 77 bp sequence that was repeated four times, and a 71 bp sequence that was repeated six times. Strix aluco, however, had only one repeated sequence, a 78 bp sequence that was repeated five times. The results of this study seem to indicate that these tandem repeats may have resulted from slipped-strand mispairing during mtDNA replication. Moreover, there are many conserved motifs within the repeated units. These sequences could form stable stem-loop secondary structures, which suggests that these repeated sequences play an important role in regulating transcription and replication of the mitochondrial genome.This work was supported by the National Natural Sciences Foundation of China (No. 30470936)

凝胶推进剂模拟液静电雾化行为规律研究

针对凝胶推进剂雾化困难的问题,引入静电喷射技术雾化凝胶推进剂。以水凝胶模拟液为介质,探究静电雾化技术的可行性及试验条件,当收集距离2~5 cm、供液速率10~30μL/h时,凝胶模拟液可实现稳定雾化,收集液滴直径小于100μm,散射角分布在8°~36°之间,收集液滴直径和散射角均随收集距离的增大和针管直径的减小而减小;同样条件下,凝胶粘度越小,散射角和雾化液滴直径越小。在此基础上,针对单针管推力小的问题,分析使用多针管喷头进行凝胶推进剂静电雾化的可行性及其雾化区域分布,结果表明2号凝胶模拟液用于多喷头雾化可得到良好的雾化区域分布,适用于凝胶推进系统之中。国防科技重点实验室基金项目(61427040203162704004

Neighboring / Adjacent Coupling Relationship Between Urbanization and Farmland Safety Based on the Decoupling Theory

当前我国快速城市化推进对半城市化、农村等近程区域分布的耕地产生直接、强烈的影响,也对耕地安全造成强大的胁迫效应。为此,构建城市化-耕地安全综合评; 价指标体系,分别计算城市化指数和耕地安全胁迫指数,并基于脱钩理论方法对城市化水平与耕地安全水平的近程耦合关系进行量化分析。以江苏省连云港市为例,; 定量分析20002014年间其城市化水平与耕地安全的脱钩-耦合关系及耕地安全对城市化进程的响应程度。结果表明,; 20002014年间,连云港市城市化指数持续增高,耕地安全胁迫指数波动降低;; 20002007年为强脱钩、弱脱钩、扩张负脱钩及扩张脱钩的波动状态;; 20082014年呈更加强烈的波动状态,包括20082012年的强脱钩和2013年的扩张负脱钩状态,这反映出区域发展规划、土地利用规划和城市总体; 规划的实施对这一典型近程耦合系统产生了较大影响。研究期内连云港市城市化水平大幅提高,耕地安全所受胁迫程度不断降低,两者虽总体呈强脱钩关系,但仍需; 注意近程耦合结果波动性较大所揭示的不确定性问题,需要谨慎权衡城市化相关政策对耕地安全的影响。与过去的宏观、单一指标模型相比,基于近程耦合关系提出; 的多指标脱钩-耦合模型更具综合性,能更加全面、精细化分析快速城市化进程与耕地安全保护之间的复杂关系,为半城市化地区景观安全格局评估提供支撑,为城; 市化与耕地保护之间的协调发展提供决策支持。The current rapid development of urbanization in China has directly and; seriously affected farmlands distribution in its para-urbanized; neighboring /adjacent regions,and also posed severe stresses on safety; of these land. It is,therefore,essential to build up a comprehensive; urbanization and farmland safety assessment indicator system,work out; quantitatively urbanization index and farmland safety stress index,and; analyze quantitatively neighboring coupling relations between; urbanization and arable land safety based on the decoupling theory and; method. A case study was conducted of Lianyungang City of Jiangsu; Province to explore quantitatively coupling-decoupling relationship; between urbanization and farmland safety and response of farmland safety; to the progress of urbanization during the period from 2000 to 2014.; Results show that during the study period,urbanization index of; Lianyungang City increased steadily,while farmland safety stress index; fluctuated with a declining trend. Their relationship displayed a wavy; trend from 2000 to 2007,i.e. strong decoupling-weak decoupling-expanding; negative decoupling-expanding connection and a strong fluctuation curve; from 2008 to 2014,including strong decoupling during 2008-2012 and; expanding negative decoupling in 2013,which reflects strong impacts of; the implementation of the regional development,land use and city overall; programs on this typical neighboring / adjacent coupling system. During; the study period,Lianyungang City made a huge and steady progress in; urbanization, while reducing fluctuation of its stress on farmland; safety. Though the two posed a strong decoupling relationship,the; problem of uncertainty reflected in the huge fluctuation of the result; of the neighboring /adjacent decoupling calls for high attention,and the; influence urbanization had on farmland safety needs to be weighed; prudently. Compared to the macroscopic single indicator models used in; the past,the multi-index decoupling-coupling model built for the; neighboring coupling relationship is more comprehensive and capable of; analyzing the complex relationship between rapid urbanization and; protection of farmland safety in a more holistic and finer way.; Furthermore,it may provide theoretical support to landscape safety; assessment of para-urbanizd regions and to decision-making on harmonized; development of both urbanization and protection of farmland.国家自然科学基金; 厦门大学大学生创新创业训练计划; 中国科学院大学生创新实践训练计

Diversity of bacterial community structure and its driving factors in three bays of Bohai Sea

[Background] Sustainable development of coastal ecosystem has become one of the most important concerns for people nowadays. Riverine output and anthropogenic interrupt have important impacts on the coastal environment. [Objective] In this study, we collected 12 samples from three transects including Bohai Bay, Liaodong Bay and Laizhou Bay to explore the microbial community and diversity in summer of 2015. [Methods] DNA was extracted from water samples by using DNA extraction kit. Samples were analyzed by Illumina HiSeq sequencing technology. We compared the differences among these three transects according to the analysis results. [Results] The diversity index and rarefaction curves showed significant differences among these three transects. The order of diversity value was Laizhou Bay>Bohai Bay>Liaodong Bay. The distribution of the dominant community was as follows: the proportion of Proteobacteria, Bacteroidetes, Cyanobacteria, Actinobacteria and Planctomycetes in the Bohai Bay was 39.8%, 25.7%, 22.4%, 5.85% and 4.38%, respectively. The dominant community proportion in Liaodong bay was Proteobacteria (37.8%), Bacteroidetes (25.7%), Cyanobacteria (17.8%), Actinobacteria (10.4%) and Planctomycetes (5.64%). While in Laizhou Bay there were only four dominant communities as follows: Proteobacteria (59.0%), Bacteroidetes (17.5%), Cyanobacteria (8.2%), Actinobacteria (7.88%). By using the principal component analysis (PCA) and Heatmap correlation analysis, we found that environmental factors were key roles in controlling the microbial diversity in the Bohai Sea. Among them, the concentration of nitrate was particularly significant according to the Mantel test analysis. [Conclusion] The microbial diversity in the three bays of Bohai Sea was very rich and multifarious. The population structure and species in the Laizhou Bay is the most complex and abundant among these three bays, and then it is Bohai Bay and Liaodong Bay. There was a significant correlation among microbial diversity, environmental factors and the spatial distribution. Above all, this study will provide a theoretical basis for further protection and ecological development of Bohai Sea

护士倒班前后智力活动的比较

受试者为20—35岁倒班和正常白班护士共46名。倒班组在上小夜班前和次日大夜班后分别给予一次智力活动测验,正常白班组两次测验的时间和时间间隔与倒班组相同。测验结果表明:倒班组两次测验仅在“数字符号”项目有部分显著性差异;而正常白班第二次测验在“数字符号”和“计数”两个项目,均较第一次测验取得显著进步。这提示倒班对于注意、记忆和反应操作速度可能有些影响