口蹄疫病毒3AB基因的表达及活性分析

通过重叠PCR合成口蹄疫病毒3AB基因,构建原核表达载体pGEX-5X-1/3AB,转化大肠杆菌BL21(DE3),IPTG诱导表达。重组蛋白主要以包涵体的形式表达,将包涵体洗涤溶解后,采用Na2+离子金属螯合亲和层析柱纯化,逐步透析法复性。ELISA实验表明,目的蛋白能与猪口蹄疫阳性血清发生特异性反应。本研究为建立以基因工程产品为抗原、鉴别诊断自然感染和免疫动物的方法提供了技术条件

Electrocatalysis of the SiC particle-modified glassy carbon electrode for the oxidation of adrenaline

The electrocatalytic properties of the SiC particle-modified glassy carbon electrode (MGC) for adrenaline oxidation were studied by cyclic voltammetry (CV), double-potential step chronocoulometry (DPSCC), and electrochemical impedance spectroscopy (EIS) techniques in McIlvaine buffer solution. It was shown that the electrode modified with SiC particles markedly displayed an electrocatalytic effect on the process of the electrochemical reaction of adrenaline, i.e., the activity and the reversibility of the MGC electrode has been significantly improved. This was attributed to the adsorption effect of the electroactive adrenaline molecules on the MGC electrode surface

猪圆环病毒2型抗体免疫金标检测试纸的研制

应用酶联免疫原理和胶体金层析技术,采用特殊的生产工艺,在玻璃纤维膜包被胶体金标记PCV-2抗原,在硝酸纤维素膜上检测线和对照线处分别包被PCV-2抗原和兔抗PCV-2抗体,制成猪圆环病毒2型抗体免疫金标检测试纸。当待检样品阳性时,在检测线处形成抗原抗体的免疫复合物而凝聚显色;当待检样品阴性时,检测线处不形成抗原抗体免疫复合物不显色。整个试验过程只需15min。试纸与ELISA试剂比较,两者都具有微量、特异、准确的优点,且金标试纸独具操作方便、快速和结果直观、容易判定的优点

A Preliminary Study on the Electrochemical Fabrication and Electrical Measurements of Semiconductor Nanostructures on Microchips

纳米电子学是纳米科技的重要科学基础，将成为21世纪信息时代的科学核心。纳米电子学的重要研究内容包括纳米结构的加工制造技术和具有量子效应的电子器件的设计与开发。随着器件尺寸的减小，纳米电子器件将会出现一些具有特征的基本现象，如电导量子化、库仑阻塞、负微分电阻、整流效应、开关效应、量子相干效应等。纳米电子器件在结构上有一个共同的特点，即它们都有一个尺寸在5~100nm之间的由半导体或金属纳米材料组成的“岛”，岛被势垒所包围，以阻止电子的进出。纳米电子器件的制造通常被描述为遵循两条路径：自上而下(top-down)和自下而上(bottom-up)。前者主要依赖于传统的半导体微／纳机械加工技术（MEM...Nanoelectronics is an important area of research in nanotechnology andnanoscience, in which nano structures with electronics functionality are designedand fabricated. Nanomater-scale devices, as the next generation devices of theelectronics, have experienced worldwild attention and rapid development recently.Nanoelectronic devices approach the namometer and the molecular scale, the bulkproperties ...学位：博士后院系专业：化学化工学院化学系_应用化学学号：XH200300

Self-assembled monolayer of o-aminothiophenol on Fe(110) surface: a combined study by electrochemistry, in situ STM, and molecular simulations

Electrochemical measurements, in situ scanning tunneling microscopy (STM) observation, and molecular mechanics (MM) simulations were performed to study the physiochemical properties such as the corrosion-inhibition effect and the optimal packing structure of o-aminothiophenol (OATP) self-assembled monolayer (SAM) formed on Fe(110) surface in 0.1 M NaClO4 solution. The formation of OATP SAMs drastically depressed the Faradaic processes at the Fe(110) surface and reduced the electrical double-layer capacitance at the electrode/electrolyte interface, revealing the anticorrosion property and the blocking behavior of OATP adlayers. Two-dimensional ordered molecular arrays of OATP on Fe(110) surface with a p(2 x 2) commensurate structure were observed by STM measurements. MM calculations showed that the p(2 x 2) packing pattern is indeed the preferable structure for OATP molecules adsorbed on Fe(110), in accordance with STM experiments. The OATP SAM on Fe(110) is 0.50 nm in thickness and with a dielectric constant of similar to7.0. (C) 2004 Elsevier B.V. All rights reserved

猪圆环病毒2型ORF1和ORF2基因的克隆与表达

根据GenBank猪圆环病毒2型基因序列,设计合成两对特异性引物,对厦门株-1进行PCR扩增,得到ORF1和ORF2基因。将PCR产物酶切后,插入到pET-22b载体中,构建原核表达载体pET-22b/ORF1、pET-22b/ORF2,转化大肠杆菌BL21(DE_3),经IPTG诱导表达,收集茵液进行SDS-PAGE分析。确定重组蛋白主要以包涵体的形式表达,包涵体洗涤溶解后,采用Ni~(2+)离子金属螯合亲和层析柱纯化蛋白,逐步透析法进行复性。Western blot和ELISA分析结果表明Rep蛋白和Cap蛋白均能与猪圆环病毒2型阳性血清发生特异性反应

微芯片上Ag_2S电化学纳米开关的构筑及电学测量的初步研究

初步探索了利用电化学方法“自下而上”地构筑了Au/Ag/Ag2S-Au固体电化学纳米开关,并确定了较适宜的开关工作条件.小于1 nm的Ag2S-Au间隔是Ag2S开关器件的关键结构,以保证电子的量子遂穿和间隔中Ag凸起的生长与收缩.测量结果表明,Ag2S开关转换具有较好的可逆性和稳定性,开关转换电流相差3个数量级以上