Asiatic clam (Corbicula fluminea)–based dynamic biosensor for real-time in situ monitoring waterborne metals

本論文提出一嶄新淡水亞洲蜆為基礎之線上生物監測系統作為生物鑑定工具，提供一即時且有效低成本量測法以偵測自然水資源中銅或鎘濃度。本系統引用亞洲蜆每日貝殼開闔韻律活動變化作為生物終點。本系統之合成乃根據生物預警系統之基本原理建置，主要有三階段;首先採用一以機率為基礎之方法模式去描述亞洲蜆暴露於重金屬銅或鎘下之貝殼開闔行為律動變化。由前人發表之文獻資料重新分析亞洲蜆對暴露於重金屬下之開闔行為反應，並以一經驗三參數希爾模式重新建構且描述出亞洲蜆隨反應時間改變的劑量反應動力方程式。結合亞洲蜆隨反應時間改變的劑量反應動力方程式、時變之半數受影響的效應濃度(EC50)方程式及以一三參數對數常態特性所迴歸之亞洲蜆每日開闔行為律動的函數，可成功地模擬亞洲蜆暴露於含有重金屬銅及鎘的水域中，隨時間變化的貝殼開闔律動反應。接著以此機率為基礎之方法結合亞洲蜆時變的劑量與開闔行為反應關係，建構出一以圖控式程式語言(LabVIEW 7.0)所編譯而得的動態蜆機制，藉此達到估測水域中時變之重金屬銅及鎘濃度變化，並提供即時檢測生物毒性效應之技術。 第二階段採用可描述生物可獲取率之生物配體模式以聯結重金屬銅的急性毒對亞洲蜆開闔行為所造成的毒性效應。藉由重新分析過去文獻中所發表之亞洲蜆馴養在實驗室中所獲得每天律動的資料及其劑量與開闔行為反應之動力關係，顯示以此生物配體模式為基礎的希爾關係方程式能更佳地描述出水域中自由活性銅離子濃度與亞洲蜆開闔反應之間的關係。本論文所提出亞洲蜆銅毒性效應與生物配體之整合模式，顯示水域自由活性銅離子能鍵結在特定的生物配體(亞洲蜆的鰓)上並降低貝類開闔濾食行為，並指出引起特定之生物效應乃需一定程度金屬累積於此生物配體上。利用此推導之亞洲蜆銅毒性效應與生物配體之整合模式，並依據當地水質條件可預測水域中時變之半數受影響的效應濃度EC50方程式及在任何時間金屬銅加入下亞洲蜆律動變化反應關係，此種方式顯現出此模式有潛力應用於發展成一生物監測系統當作一生物鑑定工具進行線上量測水域系統中銅濃度。 在第三階段本研究整合水化學原理及以生物配體模式為基礎之特定pH下時變劑量反應模式，結合所擬合之每日亞洲蜆開闔律動函數，發展一具亞洲蜆銅毒性效應與生物配體整合模式之動態蜆程式機制，可模擬亞洲蜆在不同水域中暴露於含銅環境下，其時變之開闔律動狀態。本研究之動態蜆程式機制加入因水溫及酸鹼值而異之水域環境條件補償機制，可精確地完整反應該動態蜆之銅毒檢測機制對實際環境狀態之適用性。此動態蜆合成機制係經由LabVIEW程式語言在個人電腦上模擬驗證系統之功能性，其系統測試結果顯示該程式機制在水溫及酸鹼值變動影響下，均能間接地以亞洲蜆之開闔反應去推估水中時變之銅離子活性並進一步計算因地而異之銅濃度。 在本研究中此貝類開闔量測技術為基礎之亞洲蜆生物監測系統，其設計模擬上所運用之虛擬儀控技術大大地降低在建構過程中之開發成本、設計時間及錯誤。此外，藉由增加此調整系統中時間反應及門檻濃度之重要功能，可改善即時的金屬毒性檢測技術。最後本論文所完成之以亞洲蜆銅毒性效應與生物配體之整合模式為基礎的動態蜆合成，對於銅毒性的線上生物監測提供了更佳之量化判斷，並可輔助技術發展隨地而異的生物預警系統，保護當地水域生態環境，且有助於養殖亞洲蜆管理策略之應用。The goal of this dissertation is to develop a novel freshwater clam Corbicula fluminea-based on-line bimonitoring system as a bioassay tool to offer a real-time and cost-effective method to measure copper (Cu) and cadminm (Cd) concentrations in natural water resources. The proposed system used sublethal changes in the daily valve closing activites of C. fluminea as a biological endpoint and built upon the basic principles of biological early warning system (BEWS) model in three phases. In the first phase, a probabilistic-based approach describing the valve closure behavior of C. fluminea in response to Cu and Cd was developed. The valve closure response data from published literature was reanalyzed to reconstruct the response time-dependent dose-response profiles based on an empirical three-parameter Hill equation model. The reconstructed dose-response profiles and EC50-time relationships associated with the fitted daily valve opening/closing rhythm characterized by a three-parameter lognormal function were integrated to successfully predict the time-varying bivalve closure rhythm in response to waterborne Cu/Cd. A probabilistic-based methodology associated with the time-varying dose-response relationships of valve closing behavior is incorporated into the mechanisms of a dynamic clam compiled by a LabVIEW graphic control program language in a personal computer (PC). It allows the parsimony estimation of the time-varying waterborne Cu/Cd concentrations for on-line providing the performances of the toxicity detection technique. Secondly, the biotic ligand model (BLM) describing the bioavailability was employed to link between acute Cu toxicity and its effect on valve closure behavior of freshwater clam C. fluminea based on the published experimental data of C. fluminea closure daily rhythm and dose-response profiles. The results show that a BLM-based Hill model best describes the free Cu2+-activity−valve closure response relationships. The proposed Cu-BLM-Corbicula model shows that the free ionic form of waterborne Cu bind specifically to a biotic ligand (i.e., clam gills) and impair normal valve closure behavior, indicating that a fixed-level of metal accumulation at a biotic ligand is required to elicit specific biological effects. The site-specific EC50(t) and valve closure rhythm at any integrated time was demonstrated to obtain a good prediction, indicating that the proposed model has the potential to develop a biomonitoring system as a bioassay tool to on-line measure waterborne Cu levels in aquatic systems. In the third phase, the principles of water chemistry were integrated into the modified BLM-based pH-specific concentration-time-response model. Through the combination of the Hill model-based dose-time-response function and the fitted daily rhythm function of valve closure, the constructed Cu-BLM-Corbicula-based programmatic mechanism can be used to simulate the valve closure rhythm exposed to copper in various time-varying scenarios. The compensatory mechanism under temperature-specific and pH-specific aquatic environmental conditions was incorporated into the constructed Cu-BLM-Corbicula model-based dynamic Cu detection mechanism to precisely and completely reflect the suitability for practical environmental statuses. The performance for a system testing in a dynamic clam synthesis was also demonstrated by employing a LabVIEW software in a PC. The simulation results reveal that the developed Cu-BLM-Corbicula-based programmatic mechanism can be used to indirectly estimate the time-varying waterborne Cu ion activity under the influence of water temperature and pH to further evaluate the local-specific waterborne Cu concentration. In the present study, an important system function for adjusting the time response and threshold concentration was added to improve the real-time metal toxicity detection technique. The virtual instrumentation techniques to design and simulate a C. fluminea-based biomonitoring system based on a valvometric conversion technique were adopted to greatly reduce the costs, development time and errors in implementing procedures. This proposed Cu-BLM-Corbicula model-based dynamic clam synthesis has been completed to provide a better understanding to quantify on-line measurement of Cu toxic effect on bivalve health to technically assist in developing a defensible site-specific BEWS for the protection of aquatic ecosystems, and may foster applications in clam farm management strategies.TABLE OF CONTENTS 口試委員審定書 i 誌謝 ii 中文摘要 vi Abstract viii Table of Contents xi List of Tables xiv List of Figures xv Nomenclature xxiv Chapter 1 Introduction 1 Chapter 2 Background and Research Objectives 2 2.1 Background 2 2.2 Research objectives 5 Chapter 3 Literature Review 6 3.1 Biomonitoring systems 6 3.2 Freshwater clam (Corbicula fluminea) 10 3.3 Biological response behavior 15 3.4 Mathematical models 25 3.4.1 Toxicodynamic model 25 3.4.2 Free ion activity model 27 3.4.3 Biotic ligand model 32 Chapter 4 Materials and Methods 37 4.1 Model development 37 4.1.1 Data reanalysis 37 4.1.2 Effect analysis 39 4.1.3 Exposure analysis 41 4.1.4 Statistical analyses 42 4.1.5 Mechanism development 43 4.1.5.1 Algorithm 43 4.1.5.2 Methodology 43 4.1.5.3 Dynamic artificial clam 47 4.2 BLM-based detection mechanism 54 4.2.1 Model construction 58 4.2.2 Model prediction 60 4.2.3 Uncertainty analysis 61 4.2.3.1 Model parameterization 61 4.2.3.2 Monte Carlo analysis 62 4.3 Clam-based biosensor synthesis 63 4.3.1 Principles and methodologies 63 4.3.2 Dynamic clam responsive mechanism 66 4.3.3 Cu-BLM-Corbicula model-based dynamic detection mechanism 69 4.3.4 Clam-based biological early warning system 72 Chapter 5 Results and Discussion 78 5.1 Effect and exposure assessments 78 5.2 Response predictions 83 5.2.1 Bivalve monitoring interface design 83 5.2.2 System testing 88 5.3 Primary detection mechanism 93 5.3.1 Data interpretation 93 5.3.2 Practical Operation 97 5.3.2.1 Suitability 97 5.3.2.2 Rapidity and sensitivity 98 5.4 Enhanced detection mechanism 100 5.4.1 Field measurements 100 5.4.2 Cu-BLM-corbicula-based dose-response relationships 103 5.4.3 Predicted site-specific EC50(t) and valve closure behavior 108 5.4.4 Alternatives 111 5.4.5 Applications 113 5.5 Cu-BLM-Corbicula-based dynamic clam synthesis 115 5.5.1 Windows of practical simulation 115 5.5.2 Simulation of a biological early warning system 121 5.5.2.1 An alarm-threshold concentration 121 5.5.2.2 Site-specific aquatic environmental conditions 123 5.5.3 System sensitivity and dynamic response time 130 5.5.4 Surrogate response signal 134 5.5.5 System false-alarm rate 136 Chapter 6 Conclusions 137 Chapter 7 Suggestions for Future Research 140 Bibliography 141 Curriculum Vitae 15

Clinical morbidity of resorbable plates and screws for internal fixation in orthognathic surgery

published_or_final_versionDentistryMasterMaster of Dental Surger

Applications of The Equipment and System for Greenhouse Microclimates Control

本文目的在於延續溫室微氣候環控中簡易感測系統之製作與裝置。以實地裝設於宜蘭農工專校新建溫室，進行環控操作，將實際所面臨及遭遇到的技術問加以克服及設計改良。該溫室環控以溫濕度控制模式為主體寫成C語言程式，配合個人電腦多功能實驗界面卡（內含類比／數位轉換、數位／類比轉換、數位訊號輸出入及計數器等界面）、多工及放大器界面卡、固態電譯驅動卡等電腦周邊界面裝置作系統連結及運轉，加上以感溫IC(AD 590)計測室內外乾濕球、土層、披覆材內外及作物葉面等溫度並自設小型農業微氣象觀測站（包括電氣直示型通風溫濕度計測箱、太陽電池日射計、風杯～發電機風速計及感雨器）計測室外微氣象。經實地操作運轉證明，此裝置組合滿足溫室設施樣本控制系統之控制方式並有效調節室內溫濕度環境。其造價低及施工技術已有模式可循，極適合推廣至微氣候環控要求較高之精密溫室。 The purpose of this study is to install a simple sensing system for microclimates control in a fan-pad type greenhouse. The simple sensing system has been installed in a greenhouses located at Horticlture Department, National I-lan Institute of Agriculture & Technology. The system also has been operated to solve the problems such as stability and malfuction related to the system¡¦s operation. The system is mainly classified as the temperature- humidity control mode 1 and has been modified to satisfy the control requirements. A computer program written by C- Language has been created to model the temperature-humidity control process. The equipment consists of 1 Personal Computer (PC), 1 PC multifunction lab card (involve 12 analog / digital converters, 2 digital/analog converters, 1 digital signal input/output-and-counter card), 1 multiplexer & amplifier card, SSR & relay driver board, 21 temperature sensors (AD590) and some other microclimate sensors. T type thermalcouple is being substituted by AD590 type temperature sensor to measure the temperature of soil layers, cover material, dry-wct bulb (both indoor and outdoor) and the leaf surfaces ; and a significant result has been found in this study. For the purpose of measuring microclimates, the sensors which include 1 electric-directed type ventilation temperature-humidity sensor, 1 solar- cell type radiation sensor, 1 goblet- generator type wind-speed sensor and 1 rain inductor are installed in a Agricultural Meteorological Data Acqusition station. This study shows that the set-up system well controls the temperature and humidity in the greenhouses. Two other two contibutions are found from this study (1) the cost of the set-up system is as low as $10,000, and (2) the experience gained from the installation provides us much knowledge for future application. As a result, the system set-up in this study can be applied to the greenhouses which require a strictly environmental control

Theoretical Study on Rational Dosage of Prescriptions and herbs for Chronic Disease (In Chinese)

慢病的防治是我國重大公共衛生問題，中醫藥防治慢病具有獨特的優勢。本文從慢病多虛、慢病多痼、慢病致變分析中醫藥用量和治療的特點，提出慢病方藥用量理論構建的必要性和可能性。慢病中醫用量規律和治療模式的研究，對構建科學的慢病用量理論，指導慢病中醫藥治療具有積極的作用