The association between the antioxidant property and ROS system in murine corneal epithelial progenitor cells

目的：活性氧（ROS）是体内产生的一种高活性分子，低浓度可以调节细胞代谢，基因转录和磷酸酶的活性，高浓度则会导致氧化损伤。目前，有关干细胞自身特性的研究越来越受到学者们的重视，但既往文献中关于干细胞对氧化应激反应以及应激条件下细胞内相关酶活性的变化规律却很少报道。本课题聚焦于，在过氧化氢（H2O2）诱导的氧化应激条件下，活性氧生成关键因子NOX4和抗氧化酶核转录因子NRF2在小鼠角膜上皮祖细胞（TKE2）和成熟的小鼠角膜上皮细胞（MCE）中的不同作用及相关机制。 方法：利用小鼠角膜上皮祖细胞（TKE2）和成熟小鼠角膜上皮细胞（MCE）建立过氧化氢（H2O2）诱导的氧化应激细胞模型。首先我们通...Purpose: Reactive oxygen species (ROS) is a molecular of high activity produced in human body. It can regulate metabolism, gene transcription and phosphatase activity at low concentrations,while cause oxidative damage at higher concentrations.Nowadays,the stem cell propertities has increasingly aroused the public concern,but there are still few papers published about the the reponse and ...学位：医学硕士院系专业：医学院_药理学学号：2452013115356

Study on Press Infringement and Press Self-discipline Mechanism

新闻自由是公民的基本权利之一，是通过新闻传播媒介表现出来的言论、出版自由，是新闻传播媒介和公民在法律规定或认可的情况下，搜集、采访、写作、传递、发表、印刷、发行、获知新闻或其它作品的自主性状态。作为言论自由与出版自由的衍生态，新闻自由的内涵随着媒介发展和司法实践不断得以扩充和丰富，成为一项宪法性、制度性、结构性的基本权利，并被誉为“民主政治的基石”。 由于权利范围的不断扩张，直至形成滥用之势，严重损害了社会公共利益和公民个人的基本权利，尤其是对后者名誉权、隐私权的侵害，严重破坏了新闻媒介与社会公众之间的和谐关系，新闻媒介开始遭到社会公众的强烈谴责并迫使政府欲以法律手段对其行为进行规制。 在...As a basic right of citizens, freedom of press is the freedom of speech and the freedom of publication represented by mass media and a state of self-determination of mass media and citizens to collect、interview、write、publish、print、circulate、obtain the news and other production under the regulation and authorization of the law. With the development of media and judicature, the connotation of press ...学位：法律硕士院系专业：法学院法律系_法律硕士(JM)学号：20040822

Molecular cloning of the cDNA encoding prophenoloxidase from larvae of Pieris rapae and inhibitory effects of compounds on its activity

酚氧化酶(phenoloxidase，PO，EC.1.14.18.1)在昆虫的变态发育和免疫系统中起着重要的作用，是昆虫生命活动中的重要调节酶，该酶主要参与吞噬和黑化过程，以及伤口愈合和角质硬化。PPO(prophenoloxidase)是无活性的酶原，通过蛋白级联反应激活形成PO。在本研究中，我们首次克隆了来自菜青虫（鳞翅目：粉蝶科）五龄幼虫的PPO1和PPO2的cDNA全长序列。主要实验结果如下： 1.PrPPO1的cDNA全长为3307bp，编码蛋白由682个氨基酸组成，分子量为78.5kDa，PI为6.18；PrPPO2的cDNA全长为2384bp，编码蛋白由691个氨基酸组成，分子...Phenoloxidase (PO, EC.1.14.18.1) acts as an important role during the process of insect growth and immune defense. The enzyme is involved in encapsulation and melanization processes as well as wound healing and cuticle sclerotization. PO is an active form of prophenoloxidase (PPO) after proteolytic cleavage by serine proteinase(s). In this study, we have cloned two full-length cDNA of PPO1 and PPO...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2162007115205

Metabolic Response of Basal Ganglia in Copper-laden Rat to Gandouling Based on ~1H NMR Spectroscopy

采用核磁共振氢谱(1H NMR)技术与多元统计分析相结合的方法,对肝豆灵对大鼠基底节铜损伤的调节机制进行研究。24只雄性Wistar大鼠随机分为正常组、模型组和肝豆灵组,每组8只,以铜负荷法造模。从造模第7周开始,肝豆灵组大鼠以肝豆灵灌胃。数据分析显示:与正常组比较,模型组的基底节细胞凋亡指数显著增高(p<0.01);基底节组织中的谷氨酰胺、尿苷、苏氨酸含量升高(p<0.05),甘露醇、腺苷、N-乙酰天冬氨酸、谷氨酸、N-乙酰谷氨酸(NAG)、天冬酰胺、乙酸、天冬氨酸、肌醇、三磷酸腺苷(ATP)含量降低(p<0.05);与模型组相比,肝豆灵组的基底节细胞凋亡指数显著降低(p<0.01);基底节组织中的谷氨酰胺、尿苷、乙酸、天冬氨酸含量降低(p<0.05),甘露醇、腺苷、苏氨酸、N-乙酰天冬氨酸、谷氨酸、N-乙酰谷氨酸、天冬酰胺、肌醇、ATP含量升高(p<0.05)。研究结果表明,肝豆灵可影响铜负荷大鼠基底节的代谢,对铜损伤具有一定的修复作用,其机制可能是通过调节氨的解毒和兴奋性氨基酸类神经递质的代谢,干预能量代谢,恢复神经元和神经胶质细胞功能。1H NMR spectroscopy combined with multivariate statistical method was introduced in this paper to explore the regulation effect of Gandouling on basal ganglia injury in copper-laden rats. 24 male Wistar rats were randomly divided into control group,model group and Gandouling group,with8 rats in each group. The rat model was established by copper-laden method. From the 7th week of modeling,Gandouling group was given Gandouling formula via gavage. Compared with the control group,the index of apoptosis cells in basal ganglia of model group increased significantly( p < 0. 01),and glutamine,uridine and threonine in basal ganglia were increased( p < 0. 05) while mannitol,adenosine,N-acetylaspartate, glutamate, N-acetylglutamine asparagine, acetate, aspartate, myoinositol and ATP were decreased( p < 0. 05). Compared with the model group,the index of apoptosis cells in basal ganglia of Gandouling group was significantly decreased( p < 0. 01),and glutamine,uridine,acetate and aspartate were reduced( p < 0. 05) while mannitol, adenosine, threonine,N-acetylaspartate,glutamate,N-acetylglutamine,asparagine,myo-inositol and ATP were elevated( p < 0. 05). The results showed that Gandouling could influence the metabolism of basal ganglia in copper-laden rats,which has a certain therapeutic effect on damage caused by copper. The mechanism may be restoration of the functions of neurons and glial cells,through adjusting the detoxification of ammonia and metabolism of excitatory amino acid neurotransmitter and interfering the energy metab-olism.国家自然科学基金(81202691,81201143,81371639);; 福建省自然科学基金(2015Y0032

Design and Implementation of Egg Seam Recognition System Based on Visual Detection Technology

在当前社会中食品安全的重要性毋庸置疑，人们对于农副产品的品质要求越来越高,因为它们直接关系到人们的身体健康与经济利益。随着计算机技术、网络技术和信息技术的迅速发展，图像检测与处理技术几乎在各个行业，甚至在人们的日常生活中得到了广泛的应用，并发挥着越来越重要的作用。它就是研究用计算机代替人们自动地去处理大量的图像信息，解决人类视觉器官的分辨能力容易受环境、情绪、疲劳等因素的影响，从而部分代替人工劳动，大大提高生产效率。 如今社会状态下的鸡蛋几乎都是在集中状态下的工业生产方式，这也就造成的在巨大的数量基数上采用人工区分鸡蛋是否有裂缝的复杂性与困难度，采用人工区分必将耗时费力浪费大量资源。本课题重...It is undoubtedly that food safety is of great importance in the current society, and subsidiary agricultural products quality undoubtedly is directly related to people's physical health and economic interests. With the rapid development of computer technology, network technology and information technology, image processing technology are widely used in almost every industry, even in people's dail...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201023058

The Study on Effectiveness of the Minimum Wage---Taking ShenZhen as an Example

我国实行最低工资制度的基本目的是保护底层劳动者的合法劳动权益，满足低收入劳动者的基本生活需求，使他们共享经济发展的成果，并最终实现经济、社会的和谐发展。但是，随着近些年我国经济的快速发展，物价不断上涨，最低收入劳动者的生活需求不断丰富，作为保护最低收入劳动者的最低工资政策能否跟上形势的变化，究竟实效如何，能否切实地保护弱势群体的合法劳动权益，能否满足当前经济形势下最低收入劳动者的生活需求？这些都需要我们对最低工资的实效性进行实证研究。 本文首先简单地对最低工资的相关概念、法规进行相关说明，然后对国内、外最低工资的研究文献进行了简单梳理。一方面，基于生存工资理论，重点分析了最低工资的内涵；另一...The basic goal of China to carry out the minimum wage system is to protect the legitimate rights and interests of vulnerable groups,to meet the basic material needs of the vulnerable workers,to let them share the fruits of economy developments,and ultimatly realize the healthy development of economy.However,with the rapid development of economy in our country in recent years, the prices rise month...学位：经济学硕士院系专业：经济学院_西方经济学学号：1532010115203

The Study on Effectiveness of the Minimum Wage---Taking ShenZhen as an Example

我国实行最低工资制度的基本目的是保护底层劳动者的合法劳动权益，满足低收入劳动者的基本生活需求，使他们共享经济发展的成果，并最终实现经济、社会的和谐发展。但是，随着近些年我国经济的快速发展，物价不断上涨，最低收入劳动者的生活需求不断丰富，作为保护最低收入劳动者的最低工资政策能否跟上形势的变化，究竟实效如何，能否切实地保护弱势群体的合法劳动权益，能否满足当前经济形势下最低收入劳动者的生活需求？这些都需要我们对最低工资的实效性进行实证研究。 本文首先简单地对最低工资的相关概念、法规进行相关说明，然后对国内、外最低工资的研究文献进行了简单梳理。一方面，基于生存工资理论，重点分析了最低工资的内涵；另一...The basic goal of China to carry out the minimum wage system is to protect the legitimate rights and interests of vulnerable groups,to meet the basic material needs of the vulnerable workers,to let them share the fruits of economy developments,and ultimatly realize the healthy development of economy.However,with the rapid development of economy in our country in recent years, the prices rise month...学位：经济学硕士院系专业：经济学院_西方经济学学号：1532010115203

New Exploration on the reasons for prosperity of Five-character poem in Wei-jin period

魏晋时期五言诗之兴盛是中国诗歌发展中的重要一环，本文在对魏晋五言诗进行计量分析的基础上，结合前人研究成果，对其时五言诗兴盛原因做重新探究。全文共分五个部分，具体构架如下： 导论部分以逯钦立先生《先秦汉魏晋南北朝诗》为依据，对魏晋五言诗的创作情况做计量分析，概括介绍研究魏晋五言诗兴盛原因的价值和现状。 第一章探讨魏晋五言诗兴盛的外部原因。主要分析时代文化思潮和外在激励机制对魏晋五言诗兴盛的促进作用。 第二章探讨魏晋五言诗兴盛的内部原因。首先，从宏观角度看，魏晋五言诗兴盛符合文体因时更替的规律，其次，从微观角度分析五言诗的诗体优势。 第三章探讨文人选择对魏晋五言诗兴盛的促进作用，这是本文的...The prosperity of Five-character poem, which manifests in Wei-jin period in the development of Chinese poetry is an important link of wei and jin dynasty, this paper aims at the basis of wuyan poem, which manifests measurement statistics, combining previous research achievements of its thriving, reasons for that, hope to explore more far reaching conclusion. The article is divided into five parts,...学位：文学硕士院系专业：人文学院中文系_中国古代文学学号：1022008115242

Towards an Interactive Model of Online Learner’s Dictionaries with a Qualitative Analysis of the Online OALD8 as an Example

学习型词典的设计目的是帮助学生学习外语。二十世纪下半叶，由于英语教学的蓬勃发展，英语学习型词典应运而生，并逐渐为词典学界所重视。近几年，词典学界密切关注在线学习型词典的崛起，并着力于探讨如何利用在线词典提高词汇记忆和词汇习得。虽然在线英语学习型词典汇集了很多语义学、文化和语法方面的信息，但是大多数英语学习者未能充分运用这些知识，甚至不知道这些信息的存在。未能恰当使用在线词典导致英语学习者在记忆词汇时出现困难。在线英语学习型词典功能强大，因为它们不仅有无限的空间和容量，而且能为英语学习者的词汇查询提供广泛改进的可能。由于探讨二者关系的研究尚在少数，因此结合在线学习型词典，研究提升词汇习得和词汇记...Learner’s dictionaries are designed for students learning a foreign language. Due to the blossoming of teaching English as a foreign language (EFL) in the second half of the twentieth century, English learner’s dictionaries came into existence and had gradually been noticed in the lexicographic world. In recent years, the rise of online learner’s dictionaries has led to questions about how to incr...学位：文学硕士院系专业：外文学院_英语语言文学学号：1202011115264

The Application of Metabolomics for Gestational Diabetes Mellitus

妊娠期糖尿病(gdM)易造成子痫前期、羊水过多、巨大儿等多种并发症。目前在妊娠中晚期检测血糖筛查gdM,确诊患者接受治疗的时间短,母婴健康存在严重隐患。代谢组学(METAbOlOMICS)是一种定量考察生命系统受内外界刺激或基因修饰后的代谢应答规律的学科。将代谢组学的方法应用于gdM研究,有望实现gdM的早期诊断,理解gdM的致病机制,对gdM的防治和母婴预后都有重要的意义。综述代谢组学方法在gdM及相关疾病研究中的应用。Gestational diabetes mellitus(GDM) easily lead to some complications,such as preeclampsia,polyhydramnios,macrosomia,and so on.The traditional diagnostic protocols for GDM mainly rely on detecting elevated glucose levels in blood,often late in the second trimester.Therefore the treatment time for the patients is not enough and the serious risk to maternal and child health raise.Metabolomics is an approach that investigates the metabolic responses of living systems to internal or external stimuli or genetic modification.The application of metabolomics to GDM research will help to interpret the pathogenesis of GDM.In addition,it can also provide the theoretical and experimental support for prevention and treatment of GDM as well as the prognosis of mothers and their infants.The characteristics of the common detection methods and the metabolomics studies of GDM were analyzed and compared in detail in this paper,and the metabolomic studies in GDM was further reviewed.国家自然科学基金(81201143;81371639); 中央高校基本科研业务费(2013121007