The effect of homogeneous retail agglomeration upstairs on rateable values

Thesis (B.Sc)--University of Hong Kong, 2009.Includes bibliographical references (p. 99-102).published_or_final_versio

血管紧张素Ⅱ及醛固酮阻滞剂对心肌梗死大鼠骨桥蛋白表达的影响

【目的】研究血管紧张素Ⅱ及醛固酮阻滞对心肌梗死大鼠非梗死区心肌组织骨桥蛋白（OPN）表达的影响。【方法】将心肌梗死后24h存活大鼠随机分为3组：盐水组（15只，5mL／d），培哚普利组（18只，2mg／kg·d）和螺内酯组（17只，20mg／kg）。灌胃给药；另设假手术组（15只）作对照。分别于心肌梗死后6周：导管法测定左室有创血流动力学；组织学方法检测非梗死区胶原纤维沉积和心肌细胞横径；Western blot检测非梗死区心肌组织OPN表达。【结果】①假手术组大鼠心肌组织Western blot未检测到OPN表达，心肌梗死大鼠6周后非梗死区心肌组织有大量OPN表达，培哚普利及螺内酯治疗均能显著抑制该蛋白的上调，差别均有统计学意义（P均〈0．01）。②与假手术组相比，所有心肌梗死大鼠均出现显著的心肌间质纤维沉积，左室重量指数增大，非梗死区心肌细胞横径增加，差别均有统计学意义（P均〈0．01）。与盐水组相比，培哚普利及螺内酯组心肌问质纤维沉积减轻，左室重量指数及非梗死区心肌细胞横径降低，差别均有统计学意义（P均〈0．01）。③与假手术组相比，所有心肌梗死大鼠6周后左室内收缩压（LVSP）和±dp/dtmax均显著下降，左室舒张末期压（LVEDP）显著上升，差别均有统计学意义（P均〈0．01）；与盐水组相比，培哚普利与螺内酯组大鼠心功能显著改善，差别均有统计学意义（P均〈0．01）。【结论】心肌梗死后大鼠非梗死区心肌组织出现大量OPN表达及显著的心室重构；血管紧张素Ⅱ及醛固酮阻滞均能显著抑制心肌梗死大鼠OPN的表达，并能改善心肌的纤维化，改善心脏功能

铅胁迫对双孢蘑菇积累铅及抗氧化酶活性的影响

铅（Pb）是环境中最普遍的重金属污染物之一，不仅直接毒害土壤生物，破坏生态结构，还可以通过食物链迁移转化，危害人体健康，因此Pb污染的治理迫在眉睫。大型真菌具有强的金属富集能力及较高的相对年生物量，因此在对Pb污染的修复中有一定优势。本文以双孢蘑菇菌丝为研究对象，采用马铃薯葡萄糖琼脂培养的方法，研究不同浓度Pb胁迫对双孢蘑菇菌丝体的生长状况、形态以及机体内抗氧化酶系统的影响。结果表明，在实验浓度下Pb对双孢蘑菇菌丝体的生长率无显著性影响，且菌丝体中Pb含量随着培养基中Pb浓度的增加而升高，达到10.40 μg/g。扫描电镜和透射电镜结果显示，Pb胁迫下菌丝体的细胞结构受到一定破坏，细胞膜透性增加。X射线能谱分析观察得到细胞中的黑色颗粒为铅，表明Pb在细胞内的富集。菌丝体中丙二醛含量随Pb浓度的增加而增加，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）等抗氧化酶活性随着Pb浓度的增加有不同程度的反应。在酶活力达到最大时，相比对照组分别增加了56.45%、61.36%、13.08%和10.59%，说明双孢蘑菇通过调节自身抗氧化酶系统适应Pb胁迫环境，据此在Pb污染的生物修复中具有应用潜力。福建省教育厅中青年教师教育科研项目（JAT170364）;;\n国家自然科学基金项目（31530008和318704832）资

普鲁兰基肿瘤靶向性纳米粒子的制备、稳定性和体外释放

背景:普鲁兰多糖以其独特的优点在纳米递药系统领域受到越来越多的关注,但是,以普鲁兰多糖为材料进行改性制备的肿瘤靶向的纳米药物载体仍有待进一步研究与开发。目的:观察纳米粒子和载药纳米粒子的体外稳定性及所包载药物的释放特征,初步评价其作为纳米药物载体的潜力。方法:应用透析法制备乙酰普鲁兰叶酸偶合体纳米粒子,以表阿霉素为模型药物,制备乙酰普鲁兰叶酸偶合体/表阿霉素载药纳米粒子(FPA/EPI),应用储存法考察其稳定性,应用透析袋法观测体外释放特征。结果与结论:乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI的粒径分别为(204.2±10.9)nm和(273.4±11.0)nm,在蒸馏水和体积分数10%胎牛血清中表面电位均较低,乙酰普鲁兰叶酸偶合体纳米粒子在水溶液中粒径1年内未见显著改变。载药纳米粒子对所包载的药物表阿霉素进行很好地释放,pH5.0磷酸盐缓冲液中释放速度明显高于pH7.4;乙酰普鲁兰叶酸偶合体纳米粒子和FPA/EPI制备容易,稳定性好,初步说明了两种粒子可望成为新型肿瘤靶向药物递药系统

Development and application of CK-MB specific monoclonal antibodies

本研究拟建立肌酸激酶同工酶MB(CK-MB)特异性单克隆抗体(mAb)的研制方法,对抗CK-MB单抗进行评价分类及性质鉴定,并初步建立CK-MB; 定量检测试剂。以CK-MB抗原免疫BALB/c小鼠,利用常规单抗制备技术,使用间接和捕获ELISA差异筛选法筛选单抗。利用肌酸激酶同工酶(CK-; MM/BB/MB)抗原对所制备单抗的抗原识别表位进行鉴定,另通过免疫印迹法及合成CK-MM、CK-BB差异性的线性表位肽鉴定对所制备的单抗进行评; 价分类。使用双抗体夹心ELISA方法筛选检测CK-MB抗原的配对mAb,并初步建立CK-MB定量检测试剂。使用74例临床标本初步评价该试剂与罗氏; 试剂的检测一致性。最终,我们成功筛选到22株稳定分泌抗CK-MB抗体的杂交瘤细胞株,这些单抗可以分为线性、偏构象的CK-MB和CK-MM或者CK; -BB交叉的单抗以及与CK-MB特异反应的偏构象型单抗,并使用偏构象型单抗研制出CK-MB定量检测试剂,该试剂与罗氏试剂相关系数r达到0.930; 9。综上所述,本研究建立了研制CK-MB偏构象型特异性单抗的筛选方法,通过对所筛选的单抗进行分析鉴定并建立了CK-MB定量检测试剂,与罗氏试剂检; 测结果符合率高。The aim of this study is to develop creatine kinase isoenzyme MB (CK-MB); specific monoclonal antibodies (mAb), and characterize the monoclonal; antibody and further development of quantitative detection assay for; CK-MB. The BALB/c mice were immunized with purchased CK-MB antigen, then; monoclonal antibodies were prepared according to conventional hybridoma; technique and screened by indirect and capture ELISA method. To identify; the epitopes and evaluate the classification, purchased creatine kinase; isoenzyme MB (CK-MM/BB/MB) antigen was used to identify the epitopes,; with immunoblotting and synthetic CK-MM and CK-BB in different linear; epitope. A double antibody sandwich ELISA was applied to screen the mAb; pairs for CK-MB detection, and the quantitative detection assay for; CK-MB was developed. We used 74 cases of clinical specimens for; comparison of our assay with Roche's CK-MB assay. We successfully; developed 22 strains of hybridoms against CK-MB, these mAbs can be; divided into linear, partial conformational CK-MB, CK-MM or CK-BB cross; monoclonal antibody and CK-MB specific reaction with partial; conformational monoclonal antibody, and CK-MB quantitative detection; assay was developed by using partial conformational monoclonal antibody.; The correlation coefficient factor r of our reagent and Roche's was; 0.930 9. This study established a screening method for CK-MB partial; conformational specific monoclonal antibody, and these monoclonal; antibodies were analyzed and an established quantitative detection assay; was developed. The new assay had a high concordance with Roche's.厦门市科技计

The Application of Metabolomics for Gestational Diabetes Mellitus

妊娠期糖尿病(gdM)易造成子痫前期、羊水过多、巨大儿等多种并发症。目前在妊娠中晚期检测血糖筛查gdM,确诊患者接受治疗的时间短,母婴健康存在严重隐患。代谢组学(METAbOlOMICS)是一种定量考察生命系统受内外界刺激或基因修饰后的代谢应答规律的学科。将代谢组学的方法应用于gdM研究,有望实现gdM的早期诊断,理解gdM的致病机制,对gdM的防治和母婴预后都有重要的意义。综述代谢组学方法在gdM及相关疾病研究中的应用。Gestational diabetes mellitus(GDM) easily lead to some complications,such as preeclampsia,polyhydramnios,macrosomia,and so on.The traditional diagnostic protocols for GDM mainly rely on detecting elevated glucose levels in blood,often late in the second trimester.Therefore the treatment time for the patients is not enough and the serious risk to maternal and child health raise.Metabolomics is an approach that investigates the metabolic responses of living systems to internal or external stimuli or genetic modification.The application of metabolomics to GDM research will help to interpret the pathogenesis of GDM.In addition,it can also provide the theoretical and experimental support for prevention and treatment of GDM as well as the prognosis of mothers and their infants.The characteristics of the common detection methods and the metabolomics studies of GDM were analyzed and compared in detail in this paper,and the metabolomic studies in GDM was further reviewed.国家自然科学基金(81201143;81371639); 中央高校基本科研业务费(2013121007

Advertising in the PRC: local adaptation of advertising strategy addressing the needs & aspirations of Chineseconsumers

published_or_final_versionBusiness AdministrationMasterMaster of Business Administratio

环境丰容对灰大袋鼠行为的影响

对南京市红山森林动物园内圈养的19只灰大袋鼠群体进行了环境丰容试验。试验内容为观察环境丰容对灰大袋鼠行为的影响,环境丰容内容包括在外运动场堆放红土和悬挂毛绒玩具。结果表明丰容前和丰容后2 d内动物的活动、玩耍行为明显增加且差异极显著(PPP>0.05)。在观察周期内灰大袋鼠群体休息行为发生频率最高占到总行为的39%。同时分析了灰大袋鼠群体的休息、活动和社群行为发生频率和时间的关系