可溶性TAT PTD-Ngb融合蛋白的原核表达、鉴定与纯化及其对皮质神经元的跨膜转导

【目的】构建含蛋白转导域（protein transduction domain,PTD）与脑红蛋白（neuroglobin,Ngb）融合基因的表达质粒,原核表达可溶性TATPTD-Ngb,并鉴定、纯化,检测TATPTD-Ngb对皮质神经元的跨膜转导功能。【方法】提取SD大鼠脑组织总RNA,通过RT-PCR法构建编码TATPTD-Ngb的融合基因,克隆入pET28b原核表达载体,转化入大肠杆菌BL21（DE3）pLysS,诱导表达,表达产物进行SDS-PAGE、Western-blot分析及可溶性鉴定,利用所表达的目的蛋白上的6个组蛋白用Ni-NTA琼脂进行亲和层析纯化,将不同浓度纯化的融合蛋白与原代培养皮质神经元共孵育2h,用Western-blot分析融合蛋白的跨膜转导。【结果】成功构建了含有TATPTD-Ngb的原核表达载体,插入片段500bp,成功表达并纯化了可溶性TATPTD-Ngb融合蛋白,相对分子质量约为20k,Westernblot鉴定显示表达蛋白具有抗原性,并具有转导入原代培养皮质神经元的功能,随给予蛋白浓度的增高进入神经元内的融合蛋白量增高。【结论】可溶性融合蛋白TATPTD-Ngb可转导入皮 质神经元，对进一步研究Ngb的功能及蛋白转导技术的应用奠定了基础

高校大学生增强环境保护意识的方法浅析

科技进步及社会发展加大了对环境的利用，资源环境受到前所未有的压力，保护环境关系到社会未来的发展。大学生作为国家未来建设的重要力量，培养大学生环保意识，提升环保技能，有利于创造更加美好的生活环境和建设美丽新中国，对促进人与自然和谐共生有重要意义。关键词：保护环境；大学生；人与自然；和谐共生</jats:p

Complex of 3-Amino-1,2,4-Triazole and Na_2WO_4 as An Inhibitor for Brass Corrosion

应用交流阻抗和极化曲线测试3-氨基-1,2,4-三氮唑(ATA)和钨酸钠复合缓蚀剂对黄铜在3%NaCl溶液中的缓蚀作用.结果表明,ATA对黄铜有缓蚀作用,并以7.5 mg.L-1ATA的缓蚀效果最好,缓蚀率为87.46%,以7.5 mg.L-1ATA和0.15 mg.L-1Na2WO4配成复合缓蚀剂则对黄铜具有很好缓蚀协同效应,缓蚀效率达91.82%,属阴极型缓蚀剂.AC impedance and polarization methods were used to study the inhibition effect of ATA and the complex of ATA and Na2WO4 for brass corrosion in 3% NaCl solution.The results indicated that ATA had inhibition effect for brass corrosion.ATA showed the best inhibition effect for brass corrosion at the ATA concentration of 7.5 mg·L-1,and the inhibition efficiency was 87.46%.At the ATA concentration of 7.5 mg·L-1 and at the Na2WO4 concentration of 0.15 mg·L-1,the complex showed optimum inhibition effect for brass corrosion,and also showed synergistic effect and the inhibition efficiency reached 91.82%.The complex of ATA and Na2WO4 was cathodic corrosion inhibitor.作者联系地址：上海大学应用化学系;上海电力学院环境工程系,上海高校电力腐蚀控制与应用电化学重点实验室;上海电力学院环境工程系上海高校电力腐蚀控制与应用电化学重点实验室;Author's Address: 1.Department of Chemical Engineering,Shanghai University,Shanghai 200444,China;2.Department of Environment Engineering,Shanghai University of Electric Power,Shanghai 200090,Chin

Determination of the number of ψ(3686) events taken at BESIII

The number of ψ(3686) events collected by the BESIII detector during the 2021 run period is determined to be (2259.3±11.1)×106 by counting inclusive ψ(3686) hadronic events. The uncertainty is systematic and the statistical uncertainty is negligible. Meanwhile, the numbers of ψ(3686) events collected during the 2009 and 2012 run periods are updated to be (107.7±0.6)×106 and (345.4±2.6)×106, respectively. Both numbers are consistent with the previous measurements within one standard deviation. The total number of ψ(3686) events in the three data samples is (2712.4±14.3)×10^