康精汤治疗精液不液化症的临床观察

厦门市卫生局科技项目WSK052

Clinical Study of Senile Hypertension Treated by the Method of Tonifying Kidney and Activating Blood

探讨补肾活血法治疗老年性高血压病的临床疗效。治疗组30例用补肾活血法联合络活喜,对照组30例单用络活喜,观察降压疗效、症状疗效、内皮素、血栓素B2变化情况。结果显示治疗组疗效明显优于对照组,收缩压、症状积分、内皮素、血栓素B2下降明显优于对照组。提示补肾活血法能有效降低老年性高血压患者的收缩压、内皮素及血栓素B2水平,改善临床症状。To observe the curative effect of the method of tonifying kidney and activating blood in treating senile hypertension,30 patients of treatment group were treated with the prescription of tonifying kidney and activating blood plus Norvasc and 30 patients of control group were treated with Norvasc alone,the changes of blood pressure,clinical symptoms,endothelin,thromboxane B2 were observed.The results showed that the curative effect of treatment group was better,the systolic blood pressure,symptomatic scores,endothelin and thromboxane B2 were lower than that of control group.Conclusion: The method of tonifying kidney and activating blood can effectively lower systolic blood pressure,endothelin,thromboxane B2,and improve clinical symptoms of patients with senile hypertension

滇山茶提取物在化妆品中的应用

介绍了我国特色原产植物滇山茶提取物中含有的主要化学成分和提取工艺,对其在化妆品中的应用进行了综述,指出滇山茶提取物在化妆品中具有广阔应用前景和潜在经济价值

Effect of Kangquan Recipe on Apoptosis Regulatory Genes bax and bcl-2mRNA in Prostate of Rats

目的探讨康泉方对实验性大鼠前列腺组织凋亡调控基因bax mRNA、bcl-2 mRNA的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃给药30天后,处死大鼠取前列腺组织并测量湿重,RT-PCR法检测各组前列腺腹叶组织bax mRNA、bcl-2 mRNA的表达。结果与模型组比较,康泉各剂量组前列腺湿重显著下降(P0.05);与模型组比较,康泉各剂量组bax mRNA及bax/bcl-2显著升高(P0.05)。结论康泉方对大鼠良性前列腺增生有明显的治疗作用,其作用机理可能通过调节前列腺组织bax mRNA、bcl-2 mRNA表达,促进前列腺细胞凋亡,并呈明显量效关系。Objective To investigate the effect of Kangquan Recipe (KQR) on apoptosis regulatory genes bax and bcl-2 mRNA in prostate of rats. Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration. The model rats were killed and prostate glands were removed for examination after being treated with administration of KQR by gastrogavage for 30 days. The wet weight of prostate was measured and the mRNA expressions of bax and bcl-2 in rats' tissue of abdominal lobe of prostate were determined by RT-PCR. Results Compared with the model group, wet weight of prostate was lower significantly in the groups treated with different dosages of KQR (P0.05). Compared with the model group, the expressions of bax mRNA and ratios of bax/bcl-2 were significantly higher and the expressions of bcl-2 mRNA significantly lower in the KQR treated groups (P0.05). Conclusion KQR shows an obvious treatment effect on rats with benign prostatic hyperplasia, the mechanism might be through effectively regulating the expressions of bax mRNA and bcl-2 mRNA in prostatic tissue to accelerate the cell apoptosis of prostate in obvious dose-effect manner.福建省中医药科研项目(No.wzy0616);; 厦门市医学科研项目(No.WSK0526);; 厦门市卫生青年创新课题(No.WQK0510

Effect of Kangquan Recipe on Basic Fibroblast Growth Factor of Prostate Tissue in Rats

目的探讨补肾通络的康泉方对实验性大鼠前列腺组织碱性成纤维细胞生长因子(basic fibro-blast growth factor,bFGF)表达的影响。方法采用大鼠去势后注射丙酸睾丸酮致前列腺增生法造模,同时灌胃中药康泉方30天后,取大鼠前列腺组织,采用免疫组化和逆转录聚合酶链式反应(RT-PCR)法分别检测各组前列腺腹叶组织bFGF表达。结果与模型组比较,康泉高、中剂量组bFGF和bFGFmRNA表达明显降低(P0.05)。结论康泉方能够降低实验性良性前列腺增生大鼠前列腺组织bFGF表达。Objective To investigate the effect of Kangquan Recipe(KQR)on basic fibroblast growth factor(bFGF)in prostatic tissue of rats.Methods Benign prostatic hyperplasia model rat was established by injecting testosterone after castration.After being administered with KQR by gastrogavage for 30 days,the model rats were killed and their abdominal lobe prostate glands were taken for determining the protein and mRNA expressions of bFGF using immunohistochemical method and RT-polymerase chain reaction(RT-PCR)respectively.Results The expressions of bFGF and bFGF mRNA were significantly lower in the model rats being treated with high or medium dose of KQR than those in the untreated model rats(P0.05).Conclusion KQR can effectively decrease the expression of bFGF in prostatic tissue of experimental benign prostate hyperplasia model rats.福建省中医药科研项目(No.wzy0616);; 厦门市医学科研项目(No.WSK0526);; 厦门市卫生青年创新课题(No.WQK0510