Study on the sperm-mediated gene transfer technology of silkworms

本课题研究了家蚕精子介导基因转移技术（交配囊注射法〕能否有 效地将外源基因导入并整合于家蚕基因组中使之稳定遗传以及转移的夕「 源DNA浓度、线性化与否等对外源基因转移效率的影响。 研究中在同源重组绿色荧光蛋白表达载体pG350的基础上，以 pMD18-T为质粒载体，用egfp基因替换gfp基因，构建了家蚕核型多角 体病毒早期即刻蛋白基因启动于BmNPV－IE控制下的增强型绿色荧光蛋 白表达载体pMD-Fib－IE-EGFP。 在前后3年中，本研究采用本实验室建立的蚕蛾交配囊直接注射法 （将外源DNA直接注入处女蛾交配囊中再与同种雄蛾交配产卵),先后 进行了2次家蚕精子介导基因...This study is on the effiency of sperm-mediated gene transfer for foreign gene introduced into and intergrated in the silkworm's genome and inherited by the offsprings and on the influence on gene transfer of the concentration, conformation and sequence of exogenous DNA. In the study, on the basis of the homogenous recombinant plasmid pG350, an EGFP exapssion vector under the guidance of BmNP...学位：理学硕士院系专业：生命科学学院生物学系_动物学学号：19992602

Analysis of zeotropic mixtures used in OTEC Rankine cycle system

In the ocean thermal energy conversion systems, the work capacity of ammonia is strong,but it is wet working fluid, with the undesired properties of being toxic, flammable and poor security.Based on the principle of complementarity of the thermodynamic p

双浮体波能装置的功率转换特性研究

双浮体波能装置属于漂浮振荡浮子式技术的一种形式,研究其功率转换特性对于波能装置的发展具有重要意义。基于线性波理论和粘性阻尼理论,建立了装置的双自由度受迫振动方程,并以波能装置的外部阻尼作为优化变量,推导了最佳阻尼条件下波能装置吸收的最大功率表达式; 采用HydroStar 计算功率表达式中的附加质量、阻尼系数和波浪激励力,进一步求解出波能装置所吸收的功率和俘获宽度比,文中还结合实际海况分析了双浮体的主要几何参数变化对波能装置功率转换特性的影响

STUDY OF WORKING FLUID FOR CLOSED OTEC SYSTEM

The properties of working fluids have significant effect on performance of closed ocean thermal energy conversion （OTEC） Rankine cycle. With ammonia as comparative object, taking R245ca, R245fa, R236ea, R236fa, butane, isobutene, pentane and isopentane a

闭式海洋温差能发电系统的工质研究

The properties of working fluids have significant effect on performance of closed ocean thermal energy conversion （OTEC） Rankine cycle. With ammonia as comparative object, taking R245ca, R245fa, R236ea, R236fa, butane, isobutene, pentane and isopentane a

The Noninvasive DNA Extraction and Detection of Silkworms

对家蚕和蓖麻蚕的蚕蜕和蛾翅分别采用SDS裂解法和动物组织基因组试剂盒提取法提取DNA ,并与常规方法以蚕蛹提取的DNA一起进行RAPD扩增图谱比较、分析 ,结果表明用非损伤性方法提取的DNA的RAPD图谱和用常规方法提取的DNA的RAPD图谱基本一致To obtain DNA noninvasively from the silkworms Bombyx mori and Philosamia Cynthia ricini, we collected the sloughs of fifth instar and the wings of moth, and then extracted DNA by SDS and the DNA extraction kit of animal tissue and purified them. These DNA samples were analyzed by RAPD with 8 arbitrary primes. The results indicated that all the DNA samples had good quality, and that the RAPD pattern of the DNA samples from the silkworms Bombyx mori and Philosamia Cynthia ricini were respectively almost identical.国家自然科学基金资助项目 (39870 410

剥落形状对齿轮啮合刚度及动力学特性的影响

传统的剥落齿故障研究主要探讨剥落齿在不同剥落深度和不同剥落宽度的情况下齿轮刚度变化，具有较大的局限。针对此问题，对剥落形状、剥落形状分布对齿轮刚度和动力学的影响进行了研究。首先，取剥落图形在分度圆上，在此条件下取不同剥落图形。然后，在传统势能法基础上，考虑齿轮啮合位置变化时剥落宽度变化，以此为基础求出不同剥落图形的刚度变化曲线；再结合动力学变化，对于不同故障振动加速度信号进行分析，说明剥落形状因素影响齿轮相关指标，使得剥落齿轮与正常齿轮难以判别，指标相互混叠。提出使用概率密度函数与峭度值相结合的方法，利用概率密度函数曲线判断齿轮故障状态。发现此种方法可以有效抵消形状因素对于指标的干扰，具备较高可行性及判别能力

EXPERIMENTAL STUDY ON LINEAR POWER GENERATING PRINCIPLE OF FLOATING WAVE ENERGY CONVERSION

The floating direct drive wave energy device was introduced in this paper. The model of device and wave tank experiment was designed according to the similarity theory, and the effects of different wave height, different periods and different loads on ou

漂浮式波浪能直线发电原理试验研究

The floating direct drive wave energy device was introduced in this paper. The model of device and wave tank experiment was designed according to the similarity theory, and the effects of different wave height, different periods and different loads on ou