Identification and Biological Characteristics Analysis of a Wild Pleurotus pulmonarius

本研究以野外采集获得的1株野生侧耳属真菌为对象,对其进行rDNA-ITS序列扩增测序并分析后初步确定其为肺形侧耳(Pleurotus; pulmonarius),并与本实验室中现有的秀珍菇菌株序列进行随机扩增多态性DNA; (RAPD)、ISSR、相关序列扩增多态性(SRAP)的综合分析后进一步确定该野生菌株与其他菌株间具有一定的遗传距离,是一株新的秀珍菇菌株。以福; 建省认定的秀珍菇品种秀迪1号为对照菌株,对该菌株进行生物学特性进行研究,结果表明,其菌丝可在温度为5~45°C的范围内生长,最适生长温度为30°; C;菌丝可生长的pH范围为pH; 3~10,最适pH值为7。在含可溶性淀粉、酵母粉为碳氮源的培养基中,菌丝生长速度快,长势好。该野生菌株生长所需的最适碳、氮源及生长条件与对照菌株; 相似,这为其进一步的驯化栽培提供一定的理论基础。In this study, a wild pleurotus strain collected in the forest was; selected as the research object. We sequenced its rDNA-ITS sequence and; analyzed the result to preliminarily classified it as Pleurotus; pulmonarius. Also, the comparision of the random amplified polymorphic; DNA (RAPD), ISSR and sequence related amplified polymorphism (SRAP) with; the strain sequence of other available Pleurotus geesteranus in our; laboratory showed that this strain had certain genetic distance with; other tested strains, which indicated that it was a new Pleurotus; geesteranus strain. The Pleurotus pulmonarius No.l approved by Fujian; province was selected as control group, and its biological; characteristics was analyzed, the result of which showed that its; mycelium could grow at a temperature of 5~45℃, and the optimum growth; temperature was 30°C. The pH range of the mycelium growth was 3 to 10,; and the optimum pH value was 7. In the substrate containing soluble; starch and yeast powder as carbon and nitrogen source, the mycelium grew; fast and good. The most suitable carbon, nitrogen sources and growth; conditions of this wild strain were similar to the control strain, which; provided a theoretical basis for the further domestication and; cultivation of this strain.福州市农业科技项

Preparation,Identification and Purification of Antibodies Against Four GnRHs

用3-马来酰亚胺基苯甲酸琥珀酰亚胺酯(MbS)法将人工合成的4种不同来源的促性腺激素释放激(gnrH)(即章鱼gnrH、海鞘gnrH-Ⅰ、七鳃鳗gnrH-Ⅰ和七鳃鳗gnrH-Ⅲ)分别与匙孔戚血蓝蛋白(klH)偶联后作为抗原,免疫新西兰大白兔获得gnrH抗体.用间接ElISA方法检测抗血清效价,并用WESTErn blOT鉴定其特异性.制备抗原肽亲和纯化柱,纯化多克隆抗体.结果显示,4种多克隆抗体效价高达1∶500 000(体积比),并能和相应的gnrH多肽特异性结合,纯化后的抗体在SdS-PAgE显示为单一条带.通过上述方法,获得4种高效价、高纯度的gnrH抗体,为低等动物gnrH的研究提供了理论依据.Four immunogens was prepared by conjugation of four synthetic GnRH peptides(octopus GnRH,tunicate GnRH-Ⅰ,lamprey GnRH-Ⅰand lamprey GnRH-Ⅲ) and carrier protein key-hole limpet hemocyanin(KLH) respectively by MBS method.Antibody production against each immunogen was initiated in vivo by emulsifying them in Freund′s adjuvant and administering them to 2-month-old male New Zealand rabbits by hypodermal injection.The titer of antiserums was tested by indirect ELISA,and the results showed that the titer of four antibodies was 1∶500 000.The results of Western blot analysis showed that each antibody could specifically bind to the corresponding GnRH peptide.Finally,antiserums were purified by affinity purification,and the purified antibody showed a single band in SDS-PAGE.These datas suggest that four GnRH antibody with high effect and high purity were produced,and they provide an useful tool for studying GnRH of lower animal.国家自然科学基金资助项目(41076081

Immunohistochemical Study of GnRH Receptor in the Nervous Organs of the Male Swimming Crab,Portunus trituberculatus

采用MAXVISIOnTM免疫组织化学方法,以兔抗人gnrH受体的多克隆抗体,对雄性三疣梭子蟹(POrTunuS TrITubErCulATuS)的神经器官(视神经节、脑神经节和胸神经团)进行免疫组织化学定位.结果显示:gnrH受体的免疫阳性物质在雄性三疣梭子蟹视神经节、脑神经节和胸神经团的多个部位都有较为广泛的存在,定位在神经细胞的细胞质和神经髓质中,不同神经器官gnrH受体的免疫阳性强度有一定的差异.三疣梭子蟹神经器官存在gnrH受体的免疫阳性物质,为gnrH参与其神经调节作用提供了形态学依据.The immunohistochemical technique of MaxVisionTM method and human GnRH receptor antibody against rabbit were used in this study to find out the locations of immunoreactive substances in the nervous organs of the male swimming crab,Portunus trituberculatus.The results revealed that:the immunoreactive substances of GnRH receptor were widely detected in many parts of the optic ganglion,cerebral ganglion and thoracic ganglion mass of the male crab.The immunoreactive substances located in the cytoplasm of neurosecretory cells and the neuropils.In addition,there were differentions in the intensity of immunoreaction in these three nervous organs.The immunoreactive substances of GnRH receptor existed in the nervous organs could provide morphologic evidence for GnRH participating in the neuroendocrine regulation in the swimming crab.国家自然科学基金项目(40776084;41076081

Preliminary study of gonadotropin-releasing hormone receptor (GnRH receptor) in the optic ganglion of the female swimming crab (Portunus trituberculatus) by using immunohistochemical technique

【中文摘要】 为了研究促性腺激素释放激素(GnRH)受体在甲壳动物视神经节中的存在及分布情况,采用MaxVisionTM免疫组织化学方法,以兔抗人GnRH受体的多克隆抗体,对不同发育期雌性三疣梭子蟹的视神经节进行了免疫组织化学定位。结果显示,GnRH受体的免疫阳性物质在视神经节的多个部位,视神经层、视外髓、视内髓、视端髓及X器中都有较为广泛的存在,在视外髓、视内髓与X器处的神经分泌细胞中尤为明显。不同发育期的雌性三疣梭子蟹视神经节GnRH受体的免疫阳性分布位置相似,免疫阳性强度存在一定的差异。三疣梭子蟹视神经节存在的GnRH受体免疫阳性物质,为GnRH参与视神经节调节作用提供了形态学依据。 【英文摘要】 Gonadotropin-releasing hormone (GnRH) is a kind of very important and crucial hormone in neuroregulation and reproduction.In order to elucidate whether the GnRH receptor exists or not,and to investigate its locations in the crustacean-peculiar optic ganglion as well,the immunohistochemical technique of MaxVisionTM method was used in this study.The locations of immunoreactive substances in the optic ganglion of the female swimming crabs,Portunus trituberculatus were found out in different vitellogenesis stag...国家自然科学基金项目(40776084,41076081

超大孔聚合物微球的亲水改性及在快速生化分离中的应用

随着生物医药技术的高速发展,对生物下游产业中的分离纯化要求也随之提高,因此发展快速、高效的分离技术成为研究热点。分离介质作为分离纯化技术的核心一直是众多学者的关注焦点。多年来,以多糖为基质的生化分离介质是最常用的层析介质,如美国通用公司的Sepharose~(GE)和Sephadex,该介质具有生物相容性好、比表面积高、化学稳定性好等特点,但由于多糖基质的特性,其质地软、孔径小(孔径20-30nm)的性质不利于大尺寸的生物分子的快速传质~([1])。为解决这一传质问题,本课题组采用反胶团溶胀法~([2,3])制备了超大孔聚合