The suction effusion fluid is useful as a sample for blood biochemistry

皮膚を抜気吸引して得られる微量の浸出液に注目し, その生化学検査試料としての有用性を検討するために実験を行った.対象は雑種雌成犬とし, 検査項目は血漿クレアチニン, BUNに特に注目した.1)吸引に適した回路内圧は300 mmHgであり, 皮膚の同一部位で行った6時間の吸引実験では, 継続して安定した液量が得られた.2)尿毒症モデルでは, 臨床上問題となる全濃度域で, 浸出液のクレアチニン, BUN濃度は血漿濃度とよく一致した.3)吸引実験後の皮膚は1週間後に良好な角質の再生を示し, 4週間後には完全に元の状態に回復していた.4)吸引浸出液の血漿クレアチニン, BUN濃度測定試料としての信頼性は高く, とくにバイオセンサ試料として適していると考えられたWe examined whether the suction effusion fluid could be used as a new sample for blood biochemistry. The fluid was obtained from the skin surface of female mongrel dogs by transcutaneous suction after removal of the horny substance. The appropriate intraluminal pressure for suction was 300 mmHg at which the effusion fluid flow rate was 0.6 microliter/min/cm2. The blood biochemistry examination revealed that the creatinine (Cr) and urea (BUN) concentrations in the suction effusion fluid were similar to those in plasma and that the protein and lipid concentrations were much smaller than those in plasma. The six-hour study showed that the effusion fluid could be obtained for hours at the same skin area and that the Cr and BUN concentrations were consistently close to those in plasma. In uremia models, the Cr and BUN concentration in the suction effusion fluid was similar to and had an extremely high correlation (r = 0.985 and 0.982 respectively) to that in plasma through out the range for clinical practice. The skin biopsy revealed that the regeneration of the horny substance had started at one week after the suction and complete recovery was seen at four weeks. The invasiveness of the suction seemed to be small. The suction effusion fluid is a reliable material for measurement of Cr and BUN in plasma and, as it contains much less proteins and lipids, will be a good sample for biosensors

Eukarya 18S rRNA gene diversity in cryoconite on the Russel Glacier, Greenland

第32回極域生物シンポジウム12月1日（水） 国語研究所 ２階講

モノアザテトラチオエーテルを含有する温度感応性高分子による銀(I)イオンの抽出挙動

A novel temperature sensitive extraction copolymers, 9-aza-3,6,12,15-tetrathiaheptadecane-derivatized N-isopropylacrylamide polymer (ATH-NIPAAm), has been synthesized in various incorporation ratios. The concentration of monoaza-tetrathioether groups in the copolymers was determined by ICP-AES measurements. The temperature-elevated extraction experiments revealed that Silver (I) ion was extracted efficiently into the polymer phase above the lower critical solution temperature (LCST). As the ratio of monoaza-tetrathioether groups in the polymer increased, the extraction efficiency of silver (I) ion was increased

Phase synchronization between collective rhythms of globally coupled oscillator groups: noisy identical case

We theoretically investigate collective phase synchronization between interacting groups of globally coupled noisy identical phase oscillators exhibiting macroscopic rhythms. Using the phase reduction method, we derive coupled collective phase equations describing the macroscopic rhythms of the groups from microscopic Langevin phase equations of the individual oscillators via nonlinear Fokker-Planck equations. For sinusoidal microscopic coupling, we determine the type of the collective phase coupling function, i.e., whether the groups exhibit in-phase or anti-phase synchronization. We show that the macroscopic rhythms can exhibit effective anti-phase synchronization even if the microscopic phase coupling between the groups is in-phase, and vice versa. Moreover, near the onset of collective oscillations, we analytically obtain the collective phase coupling function using center-manifold and phase reductions of the nonlinear Fokker-Planck equations.Comment: 15 pages, 7 figure

Distribution and population structure of euphausiids off Adélie Land in the Southern Ocean during austral summer 2002/03, 2004/05 and 2007/08

第32回極域生物シンポジウムポスター発

Nesting behavior of Harpagifer antarcticus (Notothenioidei, Harpagiferidae) reared in an aquarium

第32回極域生物シンポジウムポスター発