Expression of recombinant hARRG cDNA in E.coli and purification of hARRG protein

目的　构建含重组人类抗砷相关基因 (humanarsenicresistencerelatedgene ,hARRG)的表达载体 ,诱导其在转化菌表达 ,分离纯化表达蛋白 ,研究该蛋白质的理化性质、抗砷功能和免疫活性 ,深入研究人类对砷化物的抵抗作用。方法　将hARRGcDNA开放阅读框亚克隆到原核表达载体Pet11C中 ,用异丙基 - β -D -硫代半乳糖苷 (IPTG)诱导表达蛋白质 ,利用阴离子交换柱Sepharose纯化蛋白质 ,SDS -PAGE胶电泳观察结果。 结果　将hARRGcDNA成功亚克隆到原核表达载体Pet11C中 ,并成功在大肠杆菌中表达 ,表达的hARRG蛋白占菌体蛋白的 5 %左右 ,该蛋白质被分离纯化。结论　原核表达载体Pet11C可以在大肠杆菌中表达hARRGcDNA ,可用阴离子交换柱Sepharose纯化抗砷相关蛋白质Objective To construct expression vector of the recombinant human arsenic resistance related gene (hARRG),induce its expression in DE\-3 and isolate and purify expression product,for studying the physiochemistry characteristic,function and immune activity of the protein,and further researching the arsenic resistant effects of human.Methods hARRG cDNA was subcloned into prokaryotic expression vector Pet11C.The recombinant protein expression was induced by IPTG,then,the protein was purified by anions Ion-exchange column Sepharose and examined by SDS-PAGE gel.Results hARRG cDNA was successfully subcloned into prokaryotic expression vector Pet11C and expressed in E.coli and the protein was purified by anions Ion-exchange column successfully.Conclusion Pet11C excpression vector containing hARRG cDNA wassuccessfully constructed,the cell DE\-3 transformed with expression vector capable of expression the gene and a hARRG protein could be purified by anions Ion-exchange column Sepharose.国家自然科学基金资助项目 (30 0 60 0 74

Catalytic Effect of Cl~- on Electrodeposition of Binary Zinc Alloys With Iron group Metals

在氯化物电解液中以定电量 (10C cm- 2 ) ,恒温 4 0℃电沉积Zn Fe族元素二元合金 .沉积产物用原子吸收光谱分析 .根据分析结果 ,作出相应的电流效率、百分含量及部分极化曲线 .镀层形貌采用扫描电镜 (SEM)观察 ,发现 :Zn Fe族元素合金的共沉积为异常共沉积 .Cl- 对铁族元素有催化作用 .随着Cl- 浓度的增加 ,降低了铁族元素的过电位 ,促进了铁族元素金属的沉积 .Zinc iron group metal alloys were deposited under coulostatic (10C.cm -2 ) at 40 ℃ chloride baths. The deposited alloys were quantitatively analyzed by Atomic Absorption Spectrometry. The current efficiency, percent content and partial polarization curves were obtained by the results. The morphology of the deposits was observed by means of Scanning Electron Microscopy(SEM). We observe that the electrodeposition of zinc iron group metal alloys is one of the best known examples of anomalous codeposition. We found the catalytic effect of Cl - on the electrodeposition behavior of iron group metal. Overpotential of iron group metal droped abruptly at high Cl - concentration, while transition current density of Zn increased gradually.作者联系地址：上海大学理学院化学系!上海宝山,200436,上海大学理学院化学系!上海宝山,200436,上海大学理学院化学系!上海宝山,200436,上海大学理学院化学系!上海宝山,200436,上海大学理学院化学系!上海宝山,200436Author's Address: Shanghai Univ., Shanghai Bao shan, 200436,Chin

Effect of SCN~- on the Transition Current of Zn during the Electrodeposition Zn-Co Alloys

　在硫酸盐电解液中的Zn＿Co合金共沉积,存在着由正常共沉积转变成异常共沉积的Zn转移电流密度,该值随着电解液中所含SCN-浓度的增加而增加,pH值的增加而减小.这主要是因为SCN-降低了Co的析出过电位,但不影响Zn及H2的析出过电位之故.For co_deposition of Zn_Co alloys at sufphate baths with micro_concentration of SCN- the transition current of Zn was found with the deposition process changing from normal Co_deposition to anomalous co_deposition, and the value of transition current increased with increasing SCN- concentration, but decreased with increasing value of pH. The existence of SCN- decreases the evolution overpotential of Co metal, but has no effects on the evolution overpotential of Zn metal and H2.作者联系地址：上海大学理学院化学系,上海大学理学院化学系,上海大学理学院化学系 上海200436 ,上海200436 ,上海200436Author's Address: Shanghai University, Shanghai 200436 Chin

Effect of Cl - on the Transition Current Density of Zn during the Electrodeposition of Zn-Ni Alloys

在氯化物电解液中Zn_Ni合金共沉积存在着由正常共沉积转变成异常共沉积的Zn转移电流密度 ,该转移电流密度随着Cl-浓度的增加而增加 ,而与溶液pH值基本无关 .这是由于Cl-的存在降低了Ni的析出过电位 ,并对Ni的析出有催化作用之故There exists a transition current density of Zn when the normal co_deposition of Zn_Ni alloys at chloride baths turns to anomalous type.The value increases with Cl - concentration, but is independent of pH, this is because the existence of Cl - decreases the evolution overpotential of Ni metal and has a catalytic effect on it.作者联系地址：上海大学理学院化学系,上海大学理学院化学系,上海大学理学院化学系 上海200436 ,上海200436 ,上海200436Author's Address: Shanghai University,Shanghai 200436,Chin

高性能辐向热压磁环及其制备方法

本发明为一种高性能辐向热压磁环及其制备方法，利用快淬方法制备的薄带破碎后在600℃～800℃热压成为全密度各向同性磁体，在温度达到700℃～900℃，通过浮动双向压制工艺该磁体热挤压成环状，热压环的取向为辐向取向，其晶粒尺寸最大至500nm。其中两步热压工艺均是在高真空下，或者达到高真空后充入氩气保护下进行的。本发明得到的磁环具有较低的氧含量，从而使磁环具有较高的磁性能。浮动双向压制减小了塑性流变的阻力，从而使磁环具有较高的磁性能和一致性，并且可以有效抑制磁环开裂