The role of jumping translocation breakpoint gene promoting instability of genome and the biological function of its protein and potential clinical value

染色体不稳定性是大多数肿瘤细胞特别是实体瘤细胞和体外转化细胞的基本特征。其表现形式包括整条染色体的获得或缺失，杂合缺失，染色体易位重排，基因扩增等。目前认为染色体不稳定是肿瘤形成的始发因素，出现在肿瘤进展的早期阶段。体内外各种损伤刺激导致的基因组DNA链的断裂是染色体不稳定的根本原因。我们的研究发现，跳跃易位断裂点基因（JTB）在胃癌、肝癌中存在广泛的基因扩增和基因易位行为，而基因的扩增和易位是DNA双链断裂后异常修复的最终结果。JTB基因的断裂易位将导致其编码跨膜结构域的基因序列丢失，从而表达出潜在分泌型蛋白。我们利用染色体步移技术鉴定出JTB基因易位后产生的融合基因的转录表达。并对潜在分泌...Chromosome instability is the basic characteristic in most tumor cells especially of solid tumor and transformed cells in vitro. It is in the form of whole chromosome gain or loss, loss of heterozygosity, chromosome translocation rearrangements and gene amplification. At present, chromosomal instability is the initial factor of tumor formation, appeared in the early stage of tumor progression. Var...学位：理学博士院系专业：医学院_生理学学号：2452011015412

HBsAg inhibit the translocation of JTB into Mitochondrion in HepG2 cell line , and play a potential role in HCC progression.

研究表明跳跃易位断裂点（JTB）基因在肝癌组织中呈现高水平表达，而在其他多种恶性肿瘤中因基因发生的不平衡转位而使其表达量下调。目前关于JTB蛋白在肝癌发生发展中所发挥的作用的研究尚不充分。我们的研究表明JTB可以与乙肝病毒表面抗原（HBsAg）相互结合，这种相互结合在肝癌发生发展中所起的作用正是我们研究的重点内容。 我们通过构建稳转细胞的方法来研究JTB与HBsAg之间的相互作用及对细胞功能学的影响，以HepG2肝癌细胞为基础建立了干扰JTB基因的HepG2细胞株及稳转HBsAg的HepG2细胞株等一系列工具细胞。主要任务在于检测各种细胞株之间抗凋亡能力的强弱及相关分子机制的研究。 我们观...Jumping translocation breakpoint (JTB) is found a significant gene expression level in malignant liver tissues. However JTB suffers an unbalanced translocation in many other types of cancer that its expression was suppressed. No comprehensive analysis on its function in human hepatocellular carcinoma (HCC) has been performed to date. We previously found that JTB protein can bind to HBV surface ant...学位：医学硕士院系专业：医学院临床医学系_内科学学号：2452008115343

Ethical Reflection on Standardized Management of Intestinal Microbiota Transplantation

肠道微生态系统是人体健康所必需的重要且复杂的生物系统。肠道微生态系统的紊乱参与人体多种疾病的发生发展过程。而肠菌移植技术可有效调节肠道菌群，修复失衡的肠道微生态，为当今多种胃肠道及胃肠以外疾病的治疗带来新的突破。然而，目前尚缺乏完整的、系统性的肠菌移植技术管理规范。就肠菌移植技术规范化管理所涉及的内容进行探讨，并从医学伦理学角度思考规范化管理中所涉及的伦理问题，以期推动肠菌移植技术的临床应用。Intestinal microecology is an important and complex biological system necessary for human health. Its disorder is involved in the development of various diseases of human body. The technology of intestinal microbio- ta transplantation can effectively regulate the intestinal flora, repair the imbalance of the intestinal microecology, and bring a new breakthrough for the treatment of many diseases of gastrointestinal tract and outside gastrointestinal tract. However, there is still no systematic and complete management standard for intestinal microbiota transplanta- tion technology. This paper discussed related content involved in standardized management of intestinal microbiota transplantation technology and reflected the ethical problems involved in standardized management from the perspec- tive of medical ethics, in order to promote the clinical application of intestinal microbiota transplantation technology