Researche into the Effects of Coastal City Travel Images on Traveler’s Satisfaction and Revisited Intention

随着我国旅游业的蓬勃发展，滨海旅游也成为旅游业新的增长点，各滨海城市都非常重视旅游资源的开发，然而国内关于滨海旅游的研究起步较晚，关于滨海旅游意象的研究也很少。本文将城市意象理论应用于滨海旅游城市开发，探讨滨海城市旅游意象与游客满意度、游客重游意向的关系。在总结梳理已有文献的基础上，提出滨海城市旅游意象与游客满意度、游客重游意向的关系模型，继而提出了关于旅游意象和顾客满意及重游意向关系的假设，并通过问卷调查，获取相关数据进行实证研究，以此问卷为测量工具，运用统计分析软件对样本进行描述性分析和信度效度检验，最后通过回归分析对研究模型进行了假设检验。得出如下结论：（一）信息资料、游客体验对认知意象...With the booming development of china's tourism, the coastal travel has become a new growth in tourism. The coastal cities attach great importance to the tourism resource development, but the domestic research on the coastal travel starts fairly late and there was little research on travel images. The thesis applies the city image theory to the tourism development of coastal city and discusses the...学位：管理学硕士院系专业：管理学院旅游系_旅游管理学号：X200715400

Consumer Behavior Research of Vegetarian Restaurant ——With an Emphasis on Eastern Coastal Areas

素食餐厅消费者行为分析是运用科学方法，有目的、有计划地系统收集、记录、整理和分析有关素食消费者市场活动状况的完整资料，为素食行业预测提供资料数据，为经营决策、制定计划提供依据。本篇论文主要通过对中国东部沿海地区素食人群随机发放调查问卷并进行SPSS数据统计分析，结合消费者行为及市场营销等理论知识，从受访者社会经济背景、素食消费行为、消费者对素食餐厅食品质量的态度及饮食行为与再次消费意愿等方面来对素食消费群体进行分析。得出如下结论： 在本次调查的受访者中，消费者以女性居多，在年龄和职业的分布上，青壮年及学生和服务业族群是素食餐厅的主要顾客，消费者的教育程度集中在大学以上学历，个人平均月收入30...Consumer behavior analysis on vegetarian restaurant is the process of collecting, recording, collating and analysing the complete data relevant to marketing activities of vegetarian consumers purposefully and designedly, to provide data for the prediction of vegetarian industry and offer accordance to the managerial decisionmaking and planning with scientific methods. This thesis analyses the vege...学位：工商管理硕士院系专业：管理学院工商管理教育中心（MBA中心）_工商管理硕士(MBA)学号：1792010115083

美国经济波动与中美贸易相关性分析

从实证的角度,检验了中国对美国的出口额与美国GDP增长率的相关程度,得出中国对美国出口额与美国经济增长率之间呈现弱的负相关并且两者之间没有经济的因果关系的结论。并且从需求与供给两方面对产生上述现象的原因进行相应的经济解释,提出了保持并深化比较优势、努力减少贸易摩擦、提高对美出口商品的档次等政策建议。教育部资助项目(03JB790003

Identification of Plant Resistance Gene with Random Forest

为了解决传统基于同源序列比对的抗性基因识别方法中假阳性高、无法发现新的抗性基因的问题,提出了一种利用随机森林分类器和k-MEAnS聚类降采样方法的抗性基因识别算法。针对目前研究工作中挖掘盲目性大的问题,进行两点改进:引入了随机森林分类器和188维组合特征来进行抗性基因识别,这种基于样本统计学习的方法能够有效地捕捉抗性基因内在特性;对于训练过程中存在的严重类别不平衡现象,使用基于聚类的降采样方法得到了更具代表性的训练集,进一步降低了识别误差。实验结果表明,该算法可以有效地进行抗性基因的识别工作,能够对现有实验验证数据进行准确的分类,并在反例集上也获得了较高的精度。The traditional homology sequence alignment based approaches usually have high false positive rate and consequently new resistance genes are difficult to be identified.This paper presents a resistance gene identification approach by applying random forest classifier and K-Means under-sampling method.In order to solve the aimless problem in gene-mining research,two main contributions are provided.Firstly,it introduces random forest and 188 dimension features to identify resistance genes,accordingly the sample statistic learning approach can efficiently capture the internal characteristic of resistance genes.Secondly,it selects a more representative training subset and reduces the identification errors for solving the serious imbalanced classification during the training process.The experimental results indicate that the approach can efficiently identify the resistance genes,not only precisely clas-sifying the existing experimental verified data,but also obtaining high accuracy on the negative sample dataset.国家自然科学基金(60932008;61172098;60871092;61001013);中央高校基本科研业务费专项资金(HIT.ICRST.2010022);高等学校博士学科点专项科研基金(201003446)---

Characteristics and trend of older men who have sex with men from 2010 to 2014 in Jiangsu province

目的:了解江苏省老年男男性行为(MSM)人群特征及变化趋势,为制定更有效的干预措施提供科学依据。方法:采用横断面调查对2010—2014年老年MSM进行监测,分析高危行为,HIV、梅毒感染状况的变化趋势。结果:共监测2010—2014年老年MSM 1 185例,浴池/桑拿/足疗/按摩等传统MSM活动场所所占比重从70.7%下降到29.3%,艾滋病相关知识知晓率、近6个月同性肛交发生率、同性肛交安全套坚持使用率分别从77.1%、55.4%、20.7%上升到91.2%、72.6%、38.5%,现症梅毒阳性率从21.7%下降到11.8%;性病、HIV阳性率未见明显下降趋势。结论:江苏老年MSM人群性活跃度高,安全套坚持使用率低,HIV阳性率维持高位。对这类人群应加强安全套推广,开展新的综合干预方式。Objective: To understand the characteristics and trend on HIV related high risk behaviors,HIV and syphilis prevalence among old men who have sex with men( MSM) in Jiangsu province from 2010 to 2014,so as to provide information for the development of intervention strategies for old MSM. Methods: Using cross sectional study,we sampled 1 185 old MSM between 2010 and 2014. We analyzed the trend on HIV related risk behaviors,HIV and syphilis prevalence. Results: The proportion of traditional sample sites,such as bath / sauna / massage room,decreased from 70. 7% to 29. 3%. The rate of knowledge,attitude,practice( KAP) related to HIV,anal sex in latest six months,and habitual condom use between old MSM increased from 77. 1%,55. 4%,20. 7% to91. 2%,72. 6%,38. 5% separately. The prevalence of syphilis decreased from 21. 7% to 11. 8%. There was no significant difference on the trend of HIV and sexually transmitted diseases. Conclusions: The rates of sexually activity and HIV prevalence were pretty high,but the rate of habitual condom use was low in old MSM. Based on the results,condom use should be strengthened and new intervention measures should be developed

Overexpression of VTE1 from Arabidopsis Resulting in High Vitamin E Accumulationand Salt Stress Tolerance Increase in Tobacco Plant~ *

维生素E在动物细胞内具有抗氧化等重要作用,但在植物体内的功能却鲜为人知.本实验利用CaMV35S启动子与来源于拟南芥的编码生育酚环化酶(TC)的cDNA(VTE1)构建的嵌合表达载体,以根癌农杆菌介导的叶盘法转化烟草W38.实验结果表明,具有卡那霉素抗性的再生植株经RT-PCR检测,得到了与阳性对照一致的495bp的目标片段;转基因植株的VE含量比对照植株高2倍左右,个别株系高达11倍.实验还发现,在耐盐性实验中转基因植株对盐的抗性明显高于野生型烟草;同时,在不同盐浓度(150、250mmol/L)胁迫下转基因植株VE含量比未转化植株增加了1.3~1.8倍,首次证明VTE1与植物耐盐性之间的关系.图7参30Vitamin E is an essential antioxidant in the human cells, while its function in plants is unknown. In this study, tobacco W38 was transformed with a construct containing a cDNA insert encoding tocopherol cyclase (VTE1) from Arabidopsis under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco plants were confirmed by RT-PCR, which yielded the same PCR product of 495 bp as the positive control. Compared to the wild type controls, up to 11 times of vitamin E were accumulated in different transgenic lines. Interestingly, the over-expression of VTE1 dramatically increased the salt stress tolerance in tobacco plants. To our knowledge, it is first reported that VTE1 can increase salt stress tolerance in plants. Fig 7, Ref 30国家自然科学基金重点项目~

Study on removal boron from solargrade silicon with hydrometallurgy

湿法提纯作为冶金法制备太阳能级硅的前处理工序,可以去除大部分金属和硼杂质。研究了以氢氟酸-硫酸混合酸为浸出剂,有机溶剂甲醇作为后处理剂,去除硅粉中硼杂质的方法。采用电感耦合等离子体发射光谱仪(ICP)等对产品进行表征。酸浸过程优化工艺条件:硫酸质量分数为55%,氢氟酸质量分数为7%,酸浸温度为70℃、酸浸时间为4 H、液固质量比为8∶1。酸浸后可使硅粉中的硼杂质质量分数由6.893x10-6降至3.867x10-6,去除率为41.9%。在酸浸基础上采用有机溶剂甲醇作为后处理剂,杂质硼质量分数降至3.84x10-6,去除率为44.29%。从硼酸浸后形成的产物入手探索提高硼去除率的方法,实验验证了该方法的可行性,为研究湿法冶金预处理太阳能级硅提供了新的参考。As a pretreatment unit for preparing solargrade silicon(SG-Si) by metallurgic method,wet purification could remove most metallic impurities and nonmetallic impurities,such as boron.Experiment researched a new method to remove boron from SG-Si with mixed hydrofluoric acid-sulfuric acid as leaching agent and with organic solvent methanol as posttreatment agent.Samples were characterized by ICP and other analysis methods.When SG-Si powder had been leached at optimized conditions as follows:mass fraction of sulfuric acid was 55%,mass fraction of hydrofluoric acid was 7%,reaction temperature was 70 ℃,reaction time was 4 h,and liquid-solid mass ratio 8∶1,it was found that mass fraction of impurity boron in SG-Si was reduced to 3.867×10-6 from 6.893×10-6 and the removal rate was 41.9% after acid leaching;on the basis of the former procedure,mass fraction of impurity boron was reduced to 3.84×10-6 and the removal rate was 44.29% when treated with organic solvent.Experiment proved the feasibility of the method and provided a new reference for researching on the pretreatment of SG-Si by hydrometallurgy

低浓度三氯乙烯诱发L-02 肝细胞蛋白质组异常表达

【: 目的】研究低剂量三氯乙烯对人 L-02 肝细胞蛋白质表达谱的影响, 以助于阐明三氯乙烯引起细 胞早期应答反应的分子机制。【方法】L-02 肝细胞暴露于低剂量( 3 μmol/L) 三氯乙烯24 h 后, 提取细胞总蛋 白, 双向电泳分离蛋白质, 软件分析凝胶图像, 基质辅助激光解吸电离飞行时间串联质谱(MALDI-TOF-TOFMS) 对相关异常变化斑点进行鉴定。【结果】和对照组比较, 低剂量三氯乙烯处理后L-02 肝细胞蛋白质表达发 生改变, 初步鉴定出7 个差异蛋白。低剂量三氯乙烯刺激后, 上调的蛋白有核糖体样蛋白( similar to ribosomal protein) 和SET protein, 下调的蛋白有异柠檬酸脱氢酶( isocitrate dehydrogenase, NADP) 和腺苷二磷酸- 核糖基 化因子鸟苷酸因子6 ( ADP-ribosylation factor guanine nucleotide factor 6) , 微管- 肌动蛋白交叉连接因子1 (microtubule-actin crosslinking factor 1) 特异表达, 肽基脯氨酰顺- 反异构酶PPI ( peptidyl prolyl cis/trans isomerase) 缺失。【结论】低剂量的三氯乙烯处理后, L-02 肝细胞中的蛋白表达谱发生明显变化, 这为三氯乙烯 毒作用机制的进一步研究提供了线索

重庆市主城区长江和嘉陵江水中有机污染物对斑马鱼胚胎仔鱼的毒性研究

目的以重庆市主城区大溪沟(嘉陵江)和寸滩(长江)两个点为代表,研究2004~2005年度重庆市水源水中有机污染物的组成和对斑马鱼胚胎仔鱼的毒性。方法固相萃取法萃取水中有机污染物,GC/MS检测有机污染物的种类;将萃取的有机污染物溶于DMSO中,并用斑马鱼胚胎仔鱼实验研究水中有机污染物对斑马鱼胚胎孵化率和仔鱼畸形率的影响。结果四个水样中均可检出有机污染物,污染物的种类以酞酸酯类和多环芳烃类为主;污染物可导致斑马鱼胚胎孵化率降低及仔鱼畸形率增加,其毒性呈现出时间和剂量依赖性;同一采样点中,枯水期水样毒性大于