Therapeutic effects comparison between platelet-rich plasma and sodium hyaluronate intra-articular injection in treatment of knee osteoarthritis

目的关节腔内注射自体富血小板血浆（PRP）与透明质酸钠（SH）,观察并比较其疗效。方法于2012年2月至12月,漳州市解放军第175医院骨科收治的81例Kellgren-Lawrence（KL）分级标准为Ⅱ、Ⅲ级膝关节骨关节炎的患者,年龄48～60岁,平均（52.6±3.6）岁;男性27例,女性53例;左膝38例,右膝43例。K-L分级标准,Ⅱ级47例,Ⅲ级34例,按就诊顺序序贯随机平行分为SH组（40例）和PRP组（41例）,分别采用SH和PRP关节腔内注射进行治疗。治疗前和治疗后3、6、9、12个月对患者进行随访并进行美国西部Ontario与Mc Master大学骨关节炎指数（WOMAC）评分评估,两组间各时间点WOMAC评分比较采用t检验,两组内各时间点WOMAC评分比较采用重复测量方差分析;分别于治疗前和治疗后12个月进行膝关节磁共振检查,评价关节腔内积液的吸收情况。结果治疗前及治疗后3个月PRP组WOMAC评分[（48.42±4.95）分,（25.38±5.12）分]和SH组[（47.17±5.12）分,（25.17±5.09）分]比较,t值分别为-0.625和0.413,差异无统计学意义（P均〉0.05）;治疗后6、9、12个月PRP组WOMAC评分[（21.44±4.73）分,（20.54±4.46）分,（16.53±7.35）分]和SH组[（25.87±5.16）分,（27.22±4.21）分,（29.63±8.21）分]比较,PRP组低于SH组,t值分别为-2.765、-3.447和-4.657,差异具有统计学意义（P均〈0.05）,治疗后12个月复查磁共振,可见PRP组的关节积液吸收例数高于SH组,差异有统计学意义（P〈0.05）。结论膝关节腔内注射自体PRP,临床效果优于透明质酸钠,中长期疗效好。Objective To compare the efficacy of platelet rich plasma （PRP） and sodium hyaluronate （SH） intra-articular injection in the patients with knee osteoarthritis of grade Ⅱand Ⅲ. Methods A total of 81 patients with knee osteoarthritis of grade Ⅱ and Ⅲ （ Kellgren-Lawrence standard） were randomly assigned to the PRP and SH group. All 81 patients were treated in the 175th hospital of PLA, Zhangzhon, with the average age of （52.6 ± 3.6） years. There were 27 males and 53 females. Forty cases in SH group and 41 cases in PRP group had got the complete follow-up. Both the two groups adopted 4 ml of 5% lidocaine for intra-articular injection. All the patients were prospectively evaluated before the treatment and at the 34 , 6th , 9th and 12th month after the treatment by the Western Ontario and McMaster Universities （WOMAC） osteoarthritis index. The swelling subsiding knee was observed. Between the two groups WOMAC scores were compared using t test at each time point, within the groups the scores were compared by repeated measures analysis of variance. The absorption of accumulated fluid in the joint cavity was observed and compared by MRI before and 12 months after the treatment. Results No serious adverse event was detected in the two groups. According to the follow-up evaluations, both the groups presented clinical improvements. The comparison between the two groups showed no statistically significant difference before therapy and three months after the treatment （ t =- 0. 625, 0. 413, P 〉 0. 05 ）. Six months, nine months and 12 months after the treatment, WOMAC score in the PRP group[ （21.44 ±4. 73）, （20. 54 ± 4. 46） ,（16. 53 ± 7. 35 ） pointsl were lower than the SH group[（25.87 ±5.16）, （27.22 ±4.21）, （29. 63 ± 8.21 ） points] ; the difference was statistically signifieant（ t = - 2. 765, - 3. 447, - 4. 657, P 〈 0. 05 ）. Conclusion PRP intra-artieular injection in the treatment of knee osteoarthritis of gradeⅡ and Ⅲ may be more suitable compared to南京军区医学科技创新重点课题项目（15ZD027

Medication rules in the treatment of digestive system neoplasms of WANG Yan-hui

目的:基于中医传承辅助平台软件,分析王彦晖教授治疗消化系统肿瘤的用药规律。方法:收集并筛选王教授于厦门燕来福国医堂治疗消化系统肿瘤病案,录入中医; 传承辅助平台软件,运用软件集成的数据分析方法,分析王教授治疗消化系统肿瘤的用药规律。结果:筛选出治疗消化系统肿瘤处方672首,分析得出其治疗消化; 系统肿瘤常用药物包括茯苓、陈皮、姜半夏、莪术、三棱、党参、白术、龙骨、牡蛎等,并演化出4首治疗消化系统肿瘤的新处方。结论:王教授治疗消化系统肿瘤; 以益气健脾、理气化痰、祛瘀散结为基本大法,扶正祛邪并重,同时根据闽南地区脾虚湿盛的致病特点重视健脾袪湿治法,并兼顾安神、止痛等治法以综合调理患者; 的机体状态。Objective: To study the medication rules in the treatment of digestive; system neoplasms of professor WANG Yan-hui using the traditional Chinese; medicine inheritance support system software (TCMISS, V2.5). Methods:; The prescriptions used by professor WANG Yan-hui in treating digestive; system neoplasms at the Yan-Lai-Fu Chinese Medicine Clinic were; collected as input data into TCMISS and were analyzed by software; integration method. Results: Totally of 672 prescriptions were selected; according to the collection standard in digestive system neoplasms; treatment. It was found that the most frequently used Chinese medicine; in these prescriptions including Tuckahoe, Pericarpium Citri; Reticulatae, Pinellia Tuber, Rhioxma Curcumae Aeruginosae, Rhizome of; Common Burreed, Root of Pilose Asiabell, Rhizome of Largehead; Atractylodes, Fossilizid, Oyster Shell, and so on. Furthermore, four new; prescriptions for treating digestive system neoplasms were created.; Conclusion: The core rules of professor WANG Yan-hui in treating; digestive system neoplasms were invigorating spleen and replenishing qi,; regulating qi-flowing for eliminating phlegm and dispelling stasis and; resolving hard mass, which means that strengthening vital qi and; eliminating pathogenic factor are equally emphasized in his; prescriptions. Besides, taking into consideration the climate; characteristics of the southern Fujian, professor WANG Yan-hui attaches; importance to invigorating spleen for eliminating dampness, combining; with tranquillization and relieving pain to treating patients; comprehensively.国家自然科学基金项目; 教育部新世纪优秀人才支持计划; 福建省自然科学基金项目; 厦门市科技计划项

溶菌酶1在小鼠超数排卵前后孵化及休眠囊胚中差异表达的研究

目的研究溶菌酶1(lysozyme 1,LYZ1)基因在超数排卵前后小鼠孵化囊胚和休眠胚胎中的分布以及表达,探究动物胚胎着床过程中新的调节机制。方法从妊娠5 d ICR小鼠体内获取的正常孵化囊胚和超排囊胚,利用小鼠延迟着床模型于妊娠第8天获取休眠胚胎和超排休眠胚胎。利用免疫荧光和Western Blot方法检测LYZ1蛋白在四组胚胎中的分布和差异表达变化。结果 LYZ1在超数排卵前、后小鼠孵化囊胚和休眠胚胎中均有表达,且主要集中在内细胞团中,滋养层细胞和胞质中少见分布;与未进行超排的小鼠相比,LYZ1蛋白在超排后小鼠胚胎中表达量显著上调,与未营造休眠模型的小鼠相比,LYZ1蛋白在休眠模型小鼠胚胎中的表达量显著上调。结论 LYZ1蛋白在囊胚内细胞团中表达,可能参与调节胚胎内细胞团的发育;LYZ1蛋白在超排-休眠胚胎中的高表达,说明LYZ1蛋白在休眠和超数排卵的双重影响下,会因为抵御不利环境而上调。2016年度科技创新服务能力建设-科技计划重点项目(KZ201610020018);;2018科技创新服务能力建设(KZ201610020018)~

内蒙古化德土城子地点晚中新世麝科化石新材料（英文）

Schlosser(1924)记述了最初发现于内蒙古化德境内或附近的二登图、敖兰卓蕾、哈尔敖包及华坝的古麝(Moschus grandaevus)。1959年中苏古生物考察队在化德的另一个地点土城子采集到不少古麝化石。2013年以来作者在土城子进行的野外发掘积累了更多的古麝化石标本。土城子标本在形态和测量数据方面和二登图等地点的古麝非常接近,可以归入同一种。根据现有资料归纳,古麝的地理分布范围自俄罗斯的西伯利亚到华北,很可能延伸到华南;其地质时代分布范围为晚中新世-上新世。食叶型古麝在土城子晚中新世地层中的出现说明当时土城子一带有森林环境。支序分析显示麝属(Moschus)化石种相互之间的系统关系非常近,可以归并为麝族(Moschini)或麝亚科(Moschinae);Micromeryx更接近麝属和鹿科,它与麝属之间的关系比以前认为的复杂,而Hispanomeryx更接近牛科。国家自然科学基金(批准号:41372027)资

可调谐相位板空域频域联合分析

<p> Wavefront coding technique is a powerful technique which overcomes the defects of traditional way to extend depth of field. By inserting a phase mask into the traditional incoherent imaging system, wavefront coding technique does not reduce the resolution and the light gathering power of the optical system but enlarges the depth of field of incoherent imaging system. Although several kinds of phase masks have been reported, cubic phase mask is still of a classical type which has been investigated widely both in spatial and frequency domain. Since the phase profiles of phase masks adopted in classical wavefront coding systems are predefined with specific optical systems, the extension of depth of field is not tunable. Tunable wavefront coding systems are introduced by using a pair of detachable phase masks, which is possible to control the depth of field and bandwidth of system by changing the position of each component with respect to the pupil center. Ojeda-Castaneda [Ojeda-Castaneda J, Rodriguez M, Naranjo R 2010 Proceedings of Progress in Electronics Research Symposium, Cambridge, July 5-8, 2010 p531] proposed to use a pair of cosine phase masks to make defocus sensitivity tunable. Zhao [Zhao H, Wei J X 2014 Opt. Commun. 326 35] investigated an improved version of Ojeda-Castaneda&#39;s design in frequency domain and found that the proposed system realized tunable bandwidth. The present study, based on the work of Zhao, analyzes the tunable characteristics of a pair of simple modified detachable cubic phase masks in spatial domain and frequency domain. Firstly, the ray aberration theory is adopted to give mathematical analyses and ray aberration maps of the proposed tunable phase mask. Based on the mathematical derivations, the size of point spread function (PSF) of system can be changed not only by profile of each cubic mask but also by the each mask displacement relative to pupil center. Secondly, a mathematical PSF based on the stationary phase method is derived in spatial domain. Simulations indicate that the positions of PSF translate in the image plane with the displacements of phase mask profile and the position of each component with respect to the pupil center. By analyzing the oscillations of PSF, the effective bandwidth is obtained. Through the expression, we can conclude that the effective bandwidth can be changed by the position, mask profile of each component and defocus. Only when the addition of two mask profiles is large enough, can the effective bandwidth be simplified without adding the influence of defocus. In addition, though the approximate expression of magnitude transfer of function (MTF) has been given by adopting stationary phase method in the appendix of previous work, it cannot give an intuitive grasp of the effective bandwidth in MTF map. Unlike the MTF expression derived before, the exact optical transfer function (OTF) expression is derived by adopting Fresnel integral in frequency domain. Exact MTF and phase transfer function (PTF) can be derived from OTF. Based on the exact MTF expression, simulations give an intuitive effective bandwidth in MTF map. Simulations also show the nonlinear property of PTF. The effective bandwidth and MTF can be changed by different phase mask profiles and positions, which indicate that the effective bandwidth and defocus sensitivity can be tuned. Analyses are conducted both in spatial domain and in frequency domain to verify the tunable property of the proposed phase mask, which provides theoretical foundation for tunable wavefront coding system design.</p