Response mechanism of phytoplankton to reduced seawater acidity/salinity and petroleum hydrocarbon pollution

浮游植物是海洋主要初级生产者，在海洋生态系统的物质循环和能量流动中起十分重要作用。由于温室效应气体CO2的加剧排放，全球变暖、海冰融化、海平面上升，导致海洋酸度和盐度下降。同时，随着海上石油开发及运输的日益发展，海洋石油泄露事故在全球各地频发，石油烃污染已成为全球主要的海洋污染物之一。 本文针对海水酸度和盐度下降及石油烃污染胁迫下，海洋浮游植物的生理生态响应及其对微量元素生物地球化学循环的影响的科学问题，以浮游植物生物量、抗氧化能力、脂质过氧化、光致产生活性氧等4种指标来探讨浮游植物的生理生态响应；首次以碳量子点(CDs)为示踪剂标记浮游植物细胞和荧光检测剂检测胞内活性氧和铜的含量；探讨海水...Phytoplankton is the main primary productivity in the marine ecosystem environment. It occupies a fundamental role in the marine ecosystem energy flow and material circulation. Increased greenhouse gas CO2 emissions lead to increased global temperatures, decreased sea ice coverage, and rose sea levels, which may lead to a decrease in seawater acidity and salinity. With the development of the petro...学位：理学博士院系专业：环境与生态学院_环境科学学号：3332013015390

Design and Implementation of a Commercial Bank CRM System Based on J2EE

国内商业银行对客户关系管理系统的建设及研究是最近这些年集中出现的，其研究背景主要是随着中国经济快速发展，各类私人业务的迅速增加而出现。长期以来，无论是银行领导还是客户经理等人员都将客户关系管理的建设停留在管理、组织及人员培训的基础上，很少从技术实现的角度来探讨客户关系管理系统的建设。 本文以客户关系管理理论为基础，借鉴国外银行的成功经验，结合国内某商业银行的实际情况，在综合银行现有业务系统的基础上，设计和实现了一个针对银行业的客户关系管理系统。论文对银行客户管理系统主要用户特点进行了分类分析，获取了系统主要功能需求及非功能性需求。结合银行客户管理的目标及原则，利用UML建模语言分别对系统分层...With China's economic development and various types of private business increased rapidly, the domestic commercial banks has become more and more focus on customer relationship management system construction in recent years. The bank leadership or customer manager staff always still put eyes on the customer relationship management theory for a long time, rarely on the design based on the mode info...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201323056

Study on the meachanism of inhibition to tyrosinase by active molecules from Amomum

酪氨酸酶(EC.1.14.18.1)是一种多亚基的含铜氧化还原酶，广泛存在于自然界中，其异常表达可引起各种各样的病变，如老年斑、雀斑、光照损伤、果蔬褐变及与人脑中的神经黑色素形成及神经退行性病变相关的帕金森病等。 砂仁为历代常用中药，是四大南药之一，在治疗胃肠道疾病中应用普遍，现代临床还用于对妇产科疾病、心力衰竭和抗肿瘤等方面的医疗，而其作用于酪氨酸酶的研究鲜有报道。本论文以砂仁为研究对象，经分级萃取得到五相萃取物，测定提取物对酪氨酸酶单酚酶和二酚酶的抑制作用机理。检测了砂仁提取物对DPPH自由基、羟基自由基和超氧阴离子自由基的清除能力及总还原力，同时研究了提取物对细菌的抑制作用。以小鼠B1...Abstract Tyrosinase (EC 1.14.18.1) is a multi-subunit of redoxidase containing copper, widely distributed in nature. Its abnormal expression is results in the various disease, such as melasama age spots, freckle, actinic damages, browning in fruits and vegetables. It also contributes to neuromelanin formation in the human brain and the neurodegeneration associated with Parkinsons disease....学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：2172008115264

Research on the System of Invalidity of the Parties in the Civil Litigation

本论文从分析民事诉讼当事人失权制度之基本理论入手，考察了该制度构建之理论基础，梳理了域外民事诉讼当事人失权制度之具体规范，并对两大法系主要国家和地区的相关规定进行了比较，最后落脚于对我国现行民事诉讼当事人失权制度进行评析，并提出了如何进行完善之建议。 本文包括引言，正文和结语三个部分。 引言部分阐释了本论文写作之意义，认为对民事诉讼当事人失权制度进行全面而深入的研究可以对民事诉讼理论的发展、规则的完善和实务的改进发挥显著的促进作用。 正文分为四章:第一章民事诉讼当事人失权制度概述，主要明确了民事诉讼当事人失权制度的定义、构成要件及其分类等要素；第二章主要从诉讼促进义务、程序安定、公正与效...ABSTRACT This essay begins with the basic theories of the system of invalidity of the parties in the civil litigation, then probes into the specific norms of the stem of invalidity of the parties in the civil litigation of foreign countries and areas, and compares the relevant provisions of the Roman law system and the common law system, analysis the existing rules of our country and put forward ...学位：法律硕士院系专业：法学院_法律硕士(JM)学号：1302010115029

传播的艺术:中美比喻说服的逻辑间距

比喻是传播中广泛使用的一种艺术.比喻中本体和喻体之间的关系体现了中美文化不同的思维方式.相对而言,中国比喻典型为直觉推理,美国比喻典型为形式逻辑.对中关大学生施测的结果,对两种比喻类型,整体思维高的中国大学生更易理解直觉推理比喻;分析思维高的美国大学生更易理解形式逻辑比喻.对中国大学生的思维方式启动实验表明,思维方式可以通过相关材料启动,并可以影响到广告中比喻的说服效果.启动整体思维的材料尚需进一步的研究.国家自然科学基金项目“东西方不同文化思维方式对广告说服的影响”(项目(71372076)的研究成果

Direct Reductive Cyanation of A 2-Pyrrolidinone Chiral Building Block Bearing An Unprotected Hydroxyl Group: A Stereoselective Synthesis of N-Methyl-2-epi-bulgecinine

报道手性合成砌块(4S,5R)-N-芐氧基-5-羟甲基-2-吡咯烷酮(3a)的直接还原氰基化及产物的立体化学与转化研究.含未保护羟基的内酰胺用L; iAlH_4/KCN体系直接还原氰基化,生成比例为69:; 31的2,5-反式/顺式非对映立体异构体.与文献类似结果对比显示,氰基负离子对5-羟甲基-△-1-吡咯啉鎗中间体的加成主要受立体电子效应和C(5; )位取代基(羟甲基)与进攻试剂间烯丙型1,3-位阻控制.该混合物在碱性条件下水解,生成比例为10:; 90的2,5-反式/2,5-顺式-bulgecinine衍生物.这一结果表明氰基水解反应伴随着在C(2)位发生了有合成价值的串联差向异构化.由此; 建立了立体选择性地合成2,5-顺式(-)-N-甲基-2-别-bulgecinine的简便方法.The direct reductive cyanation of N-benzyl-4-benzyloxy-5-hydroxymethyl-2-pyrrolidinone (3a), a lactam bearing a free hydroxyl group, has been achieved with the LiAlH4/KCN combination. The reaction afforded 2,5-trans-2-cyano-5hydroxylmethyl-4-benzyloxy-pyrrolidine (5a) and its cis-diastereomer 5b in a ratio of 69: 31 with a combined yield of 63%. The observed 2,5-trans-stereoselectivity is suggested to be resulted from both stereoelectronic effect and allylic 1,3-strain between the hydroxymethyl group at C(5) and the incoming cyanide anion on the presumed Delta-1 pyrrolinium ion intermediate. The subsequent hydrolysis of the cyano group of the diastereomeric mixture 5a/5b (trans: cis=69: 31) under basic conditions afforded the corresponding 5-hydroxymethyl-4-benzyloxyproline with 2,5-cis-diastereomer as the major diastereomer (trans: cis= 10: 90). This result implies that a synthetically useful epimerization at C(2) has occurred concomitantly. This unexpected result afforded a concise and highly stereoselective synthesis of 2,5-cis-(-)-N-methyl-2-epi-bulgecinine.National Natural Science Foundation of China [21332007]; Program for; Changjiang Scholars and Innovative Research Team in University of; Ministry of Educatio

中央苏区图书馆文献资源的采集工作研究

中央苏区时期,面对敌人的经济封锁和刚起步的图书出版发行工作,图书馆文献资源采集工作异常艰难。在党和苏维埃政府颁布的一系列法规和指令下,中央苏区的工作者明确了文献资源的采集原则,开拓多方渠道加强了文献资源采集工作,为当今图书馆文献资源采集工作提供借鉴和参考

Effects of electropuncture of acupoints on the foot Yangming stomach meridian on metabolic profiling of gastric mucosa in gastric ulcer rats

目的:采用核磁共振氢谱技术(1H; NMR)研究电针胃经穴对应激性胃溃疡大鼠胃黏膜组织代谢物谱的表达。方法:40只SD大鼠随机分为正常组、模型组、胃经组和胆经组。利用束缚-冷应激法; 制备应激性胃溃疡模型大鼠,光镜下观察大鼠胃黏膜组织病理形态学的变化,采用1H NMR技术获取胃黏膜组织1H; NMR谱,并利用模式识别方法分析各组间的代谢物谱的差异。结果:与正常组比较,模型组大鼠胃黏膜损伤和胃黏膜组织代谢轮廓差异明显,胃黏膜组织乙酸、肌; 醇、磷酸胆碱、谷氨酸、甘氨酸、乙醇胺、赖氨酸、3-羟基丁酸、缬氨酸、异亮氨酸、牛磺酸、亮氨酸和甘露醇含量上升(P<0.05),乳酸、甲硫氨酸和天; 冬氨酸含量下降(P<0.05);与模型组比较,胃经组和胆经组大鼠胃黏膜得到明显修复,胃经组大鼠胃黏膜组织代谢轮廓更接近正常大鼠,胃黏膜组织甲硫氨; 酸、天冬氨酸含量上升(P<0.05),乙酸、肌醇、磷酸胆碱、谷氨酸、甘氨酸、赖氨酸、异亮氨酸、牛磺酸、乳酸、亮氨酸和甘露醇含量下降(P<0.05; );胆经组大鼠胃黏膜组织3-羟基丁酸、甲硫氨酸含量上升(P<0.05),肌醇、磷酸胆碱、谷氨酸、乙醇胺、异亮氨酸、乳酸、亮氨酸和甘露醇含量下降(; P<0.05)。结论:电针胃经穴可调节胃溃疡大鼠胃黏膜组织特异代谢物的表达,促进胃黏膜损伤的修复。Objective: To research the effects of electropuncture of acupoints on; the foot Yangming stomach meridian on metabolic profiling of gastric; mucosa in rats of gastric ulcer induced by stressusing the 1H nuclear; magnetic resonance (1H NMR). Methods: Forty SD rats were randomly; divided into the control group, the model group, the stomach meridian; group and the gallbladder meridian group. The rat model of gastric ulcer; was induced by the restrained plus cold stress. The pathological changes; in the rat gastric mucosa tissue were observed by light microscope. The; 1H NMR spectroscopy was determined with the 1H NMR technique. And,; meanwhile the differences in the metabolic profiling were identified; with the pattern recognition method. Results: Compared to normal group,; the gastric mucosa damage and gastric mucosa metabolic profiling are; obvious, the concentration of acetic acid, choline, inositol phosphate,; glutamic acid, glycine, ethanolamine, lysine, 3-hydroxy butyric acid,; valine, isoleucine, taurine, leucine and mannitol increase and the; concentration of lactic acid, methionine and aspartic acid decrease in; model group rats (P<0.05). Compared to model group, the gastric mucosa; damage have been recovered in stomach meridian group and gallbladder; meridian group, the metabolic profiling of gastric mocusa tissue in; stomach meridian group are similar to the normal group. The; concentration of methionine, aspartic acid increase and acetic acid,; choline, inositol phosphate, glutamic acid, glycine, lysine, lactic; acid, leucine, isoleucine, taurine, and mannitol decrease in stomach; meridan group (P<0.05). The concentration of 3-hydroxy butyric acid,; methionine increase and choline, inositol phosphate, lactic acid,; leucine, isoleucine, glutamic acid, ethanol amine and mannitol decrease; in gallbladder meridian group (P<0.05). Conclusion: The electropuncture; of acupoints on the foot Yangming stomach meridian could regulate the; metabolic profiling of gastric mucosa, resulting in improving the; gastric mucosa repair of gastric ulcer rats.国家自然科学基金项目; 国家重点基础研究发展计划(973计划)项目; 广东省自然科学基金项目; 深圳市科技计划项

电针促进胃黏膜损伤修复的时效关系及分子机制

目的:动态观察电针对胃溃疡模型大鼠胃黏膜损伤修复的影响,探讨电针治疗胃溃疡的时效关系和分子机制。方法:72只SD大鼠分为空白组、模型组、胃经穴组、对照点组,并按干预时间1、4、7 d分为3个亚组,每个亚组6只。采用乙醇灌胃方法制备胃溃疡大鼠模型,胃经穴组电针\"足三里\"\"梁门\",对照点组电针\"梁门\"\"足三里\"外旁开5 mm处,每日1次,每次电针30 min。空白组和模型组用鼠板束缚但不进行电针处理,每日1次,每次30 min。用逆转录-聚合酶链反应(PR-PCR)法检测胃增殖细胞核抗原(PCNA)、P物质(SP)的表达,Western blot检测胃神经降压素(NT)的表达。结果:干预1 d后,模型组溃疡指数显著高于空白组(P0.05),PCNA m RNA、SP m RNA低于模型组(P0.05)。干预7 d后,各组以上指标组间比较差异均无统计学意义(均P>0.05)。结论:电针胃经穴能在胃溃疡发展的不同病理状态下对PCNA m RNA、SP m RNA进行双向调节的平衡作用同时促进NT蛋白高表达,进而促进溃疡的修复。深圳市科技计划项目:JCYJ 20160406140612883;;国家自然科学基金项目:8147375