Method for extracting phycocyanin

一种提取藻蓝胆素的方法，包括以下步骤：往藻蓝蛋白粉末中加入醇类溶液，避光加热回流，得到第一产物；将第一产物离心分离，取上清液，得到第二产物；将第二产物过滤后旋蒸，得到第三产物；将第三产物过滤后离心，取上清液，加入氯仿和水，静置，收集下层液体，得到第四产物；往第四产物中加入盐酸溶液，接着收集下层液体，得到第五产物；往第五产物加入盐酸溶液，混合均匀后静置，接着收集下层液体，得到第六产物；将第六产物中的氯仿吹干，得到藻蓝胆素。与从螺旋藻中天然提取藻蓝胆素相比，上述提取藻蓝胆素的方法，周期短，工艺较简单。与从表达藻蓝胆素的大肠杆菌中发酵生产藻蓝胆素相比，上述提取藻蓝胆素的方法提高了提取效率

A directional heavy groups phycocyanin trimer as application of optical sensitizing material

本发明属于生物技术，具体涉及一种定向重组别藻蓝蛋白三聚体作为光学敏化材料的应用。首先带有有利于分离纯化，和定向组装组氨酸标签；其次通过不同浓度蛋白溶液处理电极，而后采用氮气吹干或者真空冷冻干燥，依赖静电力吸附和氢键相互作用在电极表面形成定向或非定向的单层或多层吸附；敏化半导体电极或者金属电极使之在光照条件下可以直接完成太阳能到电能的转化。实现其作为新型光学敏化材料的功能。将在太阳能利用，转换和存储方面具有应用广阔的前景。并且在转化太阳能作为新一代的替代能源，为人类提供充足的电力和社会可持续发展方面有着广泛的应用

一种定向重组别藻蓝蛋白三聚体作为光学敏化材料的应用

本发明属于生物技术，具体涉及一种定向重组别藻蓝蛋白三聚体作为光学敏化材料的应用。首先带有有利于分离纯化，和定向组装组氨酸标签；其次通过不同浓度蛋白溶液处理电极，而后采用氮气吹干或者真空冷冻干燥，依赖静电力吸附和氢键相互作用在电极表面形成定向或非定向的单层或多层吸附；敏化半导体电极或者金属电极使之在光照条件下可以直接完成太阳能到电能的转化。实现其作为新型光学敏化材料的功能。将在太阳能利用，转换和存储方面具有应用广阔的前景。并且在转化太阳能作为新一代的替代能源，为人类提供充足的电力和社会可持续发展方面有着广泛的应用

RESPONSE OF GUT BACTERIAL COMMUNITY IN BLACK SEA BREAM TO DIFFERENT FEATHER MEAL IN FEED

Feather meal is a potential protein source of high nutritional value. Adding a moderate amount of feather meal into animal feed not only reduces the cost for feed but also helps re-utilizing wastes. The microbial community in the animal gut may be functionally adapted to available resources, thus play an important role in degradation and absorption of feather meal. However, little is known about the effect of feather meal amendment on the diversity and community structure of intestinal microbes in mariculture. We investigated the influence of the feather-amended meal(5%)on gut bacteria of black sea bream(Acanthopagrus schlegelii). Before adding to the meal, feather was hydrolyzed(FH),acid-treated(FA), puffed(FP), or used as raw feed(FO). Fish meal(FN)and commercially-sold feed(FS)were taken as the control. After feeding for 12 weeks, the gut bacterial diversity and communities were assessed using 16S rRNA high throughput sequencing. In general, the feather-amended treatments had significantly lower bacterial alpha diversities(OTU richness, Simpson and Shannon indices), and significantly different community structure, compared with the control. The relative abundances of Bacteroidia, Clostridia, Deltaproteobacteria, Oxalobacteraceae, and Ralstonia were significantly lower in the feather-amended treatments than in the control. There were also differences between feather-amended treatments of various styles:the proportions of Proteobacteria, Gammaproteobacteria, Oceanospirillales, Halomonadaceae,and Halomonas in the FO treatment were significantly lower than that in the FH; the proportion of Bacillales in the FO was significantly higher than that in the FN, while the proportions of Bacteroidetes, Oxalobacteraceae and Ralstonia were significantly lower. In addition, The FO treatment tended to have a higher abundance ratio of Firmicutes to Proteobacteria(7.42)than the FN(0.97)and FH(0.74). The proportions of Porphyromonadaceae and Treponema in the FN treatment were significantly higher than that in the FH. Nevertheless, Halomonas appeared to be much lower in the FN than in the FH.</p

Intelligent manufacturing process model of electric vehicle battery pack and experimental verification

According to the manufacturing and assembly process of battery pack system, this paper proposes a common process model to realize the intelligent manufacturing of electric vehicle battery pack. And the experimental verification platform is designed and built. Several key links from the actual production links were extracted to the experimental verification platform. These not only represent the typical and key process in the production and assembly of electric vehicle battery pack, but also fully reflect the automation, information and intelligence of the manufacturing. It can simulate the intelligent manufacturing process of battery pack system in a small environment and verify the process model. It can simulate the intelligent manufacturing process of electric vehicle battery pack in small environment and verify the process this paper proposes

塔克拉玛干沙漠腹地高大复杂纵向沙垄区简单横向沙丘形态特征的空间变异

为了探讨小尺度风沙地貌形态的空间变异过程,对塔克拉玛干沙漠腹地高大复杂纵向沙垄横断面上简单横向沙丘形态特征进行了研究。沙丘形态野外测量采用RTK(real-tim e k inem atic)技术,并通过南方测绘软件(South Survey)量算形态特征参数。结果表明:简单横向沙丘形态特征参数及其相互关系具有空间变异性。在8个地貌部位之间,沙丘形态特征参数(不包括迎风坡度)存在显著性差异,主要体现在:垄顶、沙垄背风坡、其他地貌部位(包括垄间地和沙垄迎风坡)三者之间的差异。地貌部位、输沙风强度以及沙丘活动强度是驱动沙丘形态空间变异的因素。沙丘高度与翼展度余弦有负对数关系,发育指数与高度、迎风坡度成负线性相关,这些相关性的显著程度在垄顶、背风坡较高,在垄间和迎风坡较低

TiN coating for inner surface modification by grid enhanced plasma source ion implantation

Using the new inner surface modification technique named GEPS II (grid-enhanced plasma source ion implantation), which is designed for inner surface modification of tubular work piece, has successfully produced TiN coating on 0.45% C steel (45 # steel). By comparing with the uncoated 45 # steel sample, the electrochemical corrosion test on the coated 45 # steel samples presents about 5-10 times improvement in their corrosion resistance. Structural analysis shows that the TiN coatings have preferred crystal orientation(111) and (2000). And the depth of coatings is about 20 nm, with part of which embedding in the substrate