例谈趣味物理学实验

啤酒是生活中常见的物品,它蕴含丰富的物理知识,启迪科学家展开不少有益的研究。引导学生关注生活中的物理现象与实验,有利于贯彻物理与社会密切联系的新课程理念

PEG定点修饰重组人睫状神经营养因子及其生物活性评价

采用分子质量为40kDa的马来酰亚胺聚乙二醇(mPEG-MAL),对重组人睫状神经营养因子突变体CNTF-C~(17)的第17位半胱氨酸巯基进行定点修饰,通过离子交换层析获得单修饰产物Mono-PEG-CNTF-C~(17),并对其结构及体内外活性进行评价。实验结果表明,在pH 7.5的Tris-HCl缓冲液体中,蛋白质与修饰剂的为1: 3,4℃下反应12h,修饰率可达到90%以上,修饰混合物通过一步阴离子交换层析可获得纯度98%以上的单修饰产物。荧光光谱(FL)及圆二色(CD)图谱显示Mono-PEG-CNTF-C~(17)与原蛋白二、三级结构一致。TF-1. CN5a. 1细胞增殖活性检测表明,Mono-PEG-CNTF-C~(17)的比活达到了6.51 × 10~5 IU/mg,体内循环半衰期相对原蛋白显著提高了30.3倍。该研究可为开发CNTF长效产品提供基础

Effect of PRECEDE-based nursing combined with individualized exercise guidance on pulmonary function and exercise capacity in patients with pulmonary tuberculosis (PRECEDE护理模式配合个体化运动指导对肺结核患者肺功能与运动能力的影响)

Objective To investigate the effect of PRECEDE-based nursing combined with individualized exercise guidance on pulmonary function and exercise capacity in patients with pulmonary tuberculosis. Methods Totally 90 patients with pulmonary tuberculosis were randomly divided into the control group and observation group, with 45 cases in each group. The control group received individualized exercise guidance, and the observation group received PRECEDE-based nursing ombined with individualized exercise guidance. The pulmonary function, exercise capacity, quality of life and satisfaction with nursing service were evaluated and compared between two groups. Results The forced expiratory volume in 1 second(FEV1), forced vital capacity (FVC) and FEV1/FVC% were increased in both two groups after intervention, and FEV1, FVC and FEV1/FVC% in the observation group were higher than those in the control group (P＜0. 01). The outcomes of 6-minute walking test (6MWT), 30-seconds sit-to-stand test (STS-30) and 30-second arm curl test(30-ACT) were increased in both two groups after intervention, and outcomes of 6MWT, STS-30 and 30-ACT in the observation group were higher than those in the control group (P＜0. 01). The St George's Respiratory Questionnaire (SGRQ) score were decreased in both two groups after intervention, and SGRQ score in the observation group were lower than that in the control group (P＜0. 01). The satisfaction degree in the observation group was higher than that in the control group(P＜0. 05). Conclusion The PRECEDE-based nursing interventions combined with individualized exercise guidance are effective to improve the pulmonary function, exercise capacity, quality of life and satisfaction of patients with pulmonary tuberculosis. (目的 探讨PRECEDE护理模式配合个体化运动指导对肺结核患者肺功能与运动能力的影响。方法 选择医院2021年10月—2023年4月收治的肺结核患者90例作为研究对象, 按随机数字表法分为对照组和观察组, 各45例。对照组给予个体化运动指导, 观察组给予PRECEDE护理模式配合个体化运动指导。比较两组的护理前后肺功能、运动能力和生活质量相关指标和量表评分变化, 评价患者护理满意度。结果 干预后两组1s用力呼气容积(FEV1), 用力肺活量(FVC)、FEV1/FVC%值均高于干预前, 且观察组高于对照组, 差异有统计学意义(P＜0. 01)。干预后两组6分钟步行试验(6MWT)、30 s坐立试验(30 s-STS)、30 s前臂屈曲试验(30-ACT)值均高于干预前, 且观察组高于对照组, 差异有统计学意义(P＜0. 01)。与干预前相比, 治疗后两组圣乔治呼吸问卷(SGRQ)评分降低, 且观察组低于对照组, 差异有统计学意义(P＜0. 01)。观察组护理满意度高于对照组(P＜0. 05)。结论 与单一个体化运动指导相比, PRECEDE护理模式配合个体化运动指导在提升肺结核患者肺功能、运动能力方面效果更佳, 能够进一步提升患者护理满意度, 改善患者生活质量。