Identification and preliminary analysis of a novel full-length cDNA encoding retinoid X receptor 2 from Schistosoma japonicum

通信作者 E-mail: [email protected][中文文摘]目的克隆编码日本血吸虫视黄酸X受体2(SjRXR2)蛋白的全长cDNA,并对其进行初步研究。方法利用cDNA末端快速扩增技术(RACE)获得SjRXR2蛋白全长编码cDNA。利用生物信息学技术,对基因结构进行初步分析。利用实时荧光定量(Real time)PCR技术对该基因在日本血吸虫不同时期虫体中的转录情况进行分析。应用在线抗体表位预测软件获得SjRXR2配体结合区抗原性较强的一个多肽序列,合成该多肽片段,并免疫小鼠制备抗血清。利用Western blot技术分析该蛋白在日本血吸虫中的表达。结果采用RACE技术成功获得了SjRXR2蛋白全长编码cDNA,总长度为5 960bp,其完整开放阅读框为4 308 bp,编码1 435个氨基酸,预测分子量为159 kDa。生物信息学分析表明该基因编码的蛋白质序列具有核受体家族2的典型结构域特征,且与曼氏血吸虫RXR2有较高的相似性。Real time PCR分析表明,该基因在21、42 d龄日本血吸虫虫体内有较高的转录水平。Western blot分析表明,小鼠SjRXR2多肽免疫血清可特异性识别日本血吸虫虫体150 kDa蛋白。结论成功获得了编码SjRXR2蛋白的全长 cDNA，并制备了针对该蛋白的特异性多克隆抗体，为进一步研究该蛋白的功能奠定了基础。 .国家自然科学基金（31172315）；公益性行业（农业）科研专项（20090303036

Study on the Coating Film of Oxidative Polymerization of Urushiol with Copper(Ⅱ) -Amine Complexes as Catalyst

用红外光谱法研究了Cu(Ⅱ)-胺配合物催化生漆漆酚的氧化聚合过程,考察了生漆漆酚成膜性能、涂膜的热稳定性和耐化学介质性能。结果表明,Cu(Ⅱ)-胺配合物催化生漆漆酚氧化聚合过程具有类似漆酶的催化反应特征,它能够催化生漆漆酚氧化聚合干燥成膜,所得的涂膜具有与天然生漆涂膜相似的硬度、附着力、抗冲击力、热稳定性以及耐化学介质性能。Abstract :The oxidative polyme rization process of urushio l wi th copper(Ⅱ) – amine complex es as cataly st were studied by IR , and the properties of coating film w ere determined. The experimental result s show ed as fo llow s :in the catalyst reaction there w ere similarit ies between copper(Ⅱ) – amine complexes and o riginal laccase , the coat ing film w as manufactured by o xidat ive po lymerizat ion wi th copper(Ⅱ) – amine complexes as cataly st , and it had good proper ties w hich we re comparable to natural urushio l co ating fi lm , such as hardness , adhesion , impact resistance , heat stability and anticorro sive to chemicals.广西教育厅资助项目(自筹);广西民族学院重点资助项目(2003年