113 research outputs found

    r DNA sequence analysis and morphological redescription of Empoasca onukii from the tea growing area of Fujian

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    重新描述了小贯小绿叶蝉的形态结构,尤其是头部色斑、翅脉、腹部内突、下生殖板及其刚毛着生位置,并增加了足部(刺毛列)特征描述。通过PCr技术克隆得到该物种的r dnA序列,包括部分18S(1 843bP)和28S序列(667 bP)以及完整的5.8S(155 bP)、ITS1(3 114 bP)和ITS2序列(1 008 bP)。序列分析表明,18S,5.8S,28S与其他物种间具有90%~95%的序列一致性;而ITS1和ITS2序列变异性非常大。碱基组成比率分析显示ITS1以及ITS2具有AT偏好性,前者A+T占66.0%,后者占65.1%。与亲缘种的比较分析显示ITS1和ITS2具有丰富的多态位点,并且ITS2更适用于近缘种的分子鉴定。The morphological structures of Empoasca onukii,the cephalic splashes,vein,abdominal apodemes,subgenital plate and the arrangement of its bristles,and the characteristics of legs were redescribed,especially the arrangement of spines were added.Ribosomal DNA( r DNA) including partial of 18S( 1 843 bp),28S( 667 bp) and complete 5.8S( 155 bp),ITS1( 3 114 bp),ITS2( 1 008 bp) were isolated by PCR technology.Sequence analysis indicated that 18 S,5.8S and 28 S had high sequence identity( 90%- 95%) with other insects; however,ITS1 and ITS2 showed much variability.The analysis of base composition showed that ITS1 and ITS2 were more preferences for AT( contained 66.0% and 65.1% A + T,respectively).Comparison with the phylogenetic species revealed that ITS1 and ITS2 from E.onukii had rich polymorphic loci and ITS2 was more suitable for molecular identification of closely related species.国家自然科学基金(81171595

    Hydrothermal synthetic ZnAl_2O_4 and Humidity sensing properties research

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    以Al2(SO4)3和zn(nO3)2为原料,采用水热法合成铝酸锌微粉体,通过扫描电镜和Xrd分析发现znAl2O4晶型良好,粒径小,呈纤维状均匀分布;进一步研究湿敏特性,结果表明:测试频率对阻抗-相对湿度(rH)特性曲线影响较大,阻抗随频率的增加而迅速减小,10kHz时在33%~95%的范围内曲线的线性度好,在相同的湿度下,试样的电容随频率的增加而减小,在低频100Hz下,电容随相对湿度的升高而增大。A simple hydrothermal was developed to fabricate ZnAl2O4 micro powder with Al2(SO4)3 and Zn(NO3)2 as raw materials,through the scanning electron microscopy(SEM)and XRD analysis found that the particles have good crystal shapesmall size,fibrous evenly distributed;Further researching the humidity sensing properties,the results show that test frequency have big effect on impedance-relative humidity(RH)characteristic curve,impedance decreases as frequency increases,the good linearity of the curve of impedance versus relative humidity appears at the frequency of 10KHZ,the capacitance of the sensor decreases as frequency increases in the same humidity and at low measurement frequency 100HZ,the capacitance increases as RH increases

    超滤膜平均孔径测算的新探讨

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    利用扫描电镜法测定超滤膜底表面上总孔面积与该底总表面积之比,再结合哈根——泊肃叶定律来推算超滤膜皮层的平均孔径。这样所得的超滤膜平均孔径比起原先单独使用扫描电镜法或哈根—泊肃叶法,可以更客观地反映超滤膜的实际参数

    Silicon-glass-based single piezoresistive pressure sensors for harsh environment applications

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    National Natural Science Foundation of China [51075344, 61274120, 51175444]; Fujian Province Major Projects on University-Industry Cooperation in Science and Technology [2013H6023]; Science and Technology Program of Xiamen [3502Z20123008, 3502Z20126006]Silicon-glass (Si-glass)-based single piezoresistive pressure sensors were designed and fabricated by standard MEMS technology. The single piezoresistive sensing element was designed to be on the lower surface of the silicon diaphragm and be vacuum-sealed in a Si-glass cavity, which form a self-packaging protection structure helpful to the applications of sensors in harsh media. The pressure sensors were fabricated using a Si-glass anodic bonding technique, and the embedded Al feedthrough lines at the Si-glass interface are used to realize the electrical connections between the piezo-sensing element and the electrode-pads, and two larger-size electrode-pads are fabricated for realizing the soldered electrical connection between the sensor and the external circuit. The performance of the pressure sensors was characterized by a pressure test system at different temperature conditions. The temperature compensation was performed by the difference between the output voltage at zero-pressure and the output at operation pressure. The measurement results show that the sensitivity is 24 mV V-1 MPa-1, the coefficient of sensitivity is 0.14% FS degrees C-1, and both the zero-point offset and the temperature coefficient of offset are equal to zero, which are able to meet the commercial application requirements. However, a nonlinearity of 5.2% FS caused by the balloon effect would considerably worsen the accuracy of the pressure sensor. It is suggested to reduce the balloon effect by using a bossed-diaphragm structure in the pressure sensor

    光子晶体光纤压力传感器稳定性研究

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    光子晶体光纤传感器的稳定性在实际工程应用中具有重要影响。文章分析了光子晶体光纤压力传感器的基本原理,介绍了光子晶体光纤压力传感器的系统组成,从理论上分析了传感器系统相对于光源波长变化、输出信号光强起伏及环境温度波动的稳定性。光子晶体光纤压力传感器激光光源的输出功率及波长的波动、敏感元件长度的改变和环境温度的变化等都对整个传感器系统的稳定性有影响

    基于分布式LiNbO<sub>3</sub>的自适应PMDC的方案设计

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    文章设计了一种快速实用的自适应偏振模色散(PMD)补偿器,主要利用x切、y向传播的L iNbO3器件的双折射特性进行了光纤链路PMD的补偿,采用一定频率范围内的功率谱密度作为补偿器的反馈控制信号,并使用了多维梯度搜索算法优化补偿量

    表达lac和glk基因的蓝藻的异养生长

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    蓝藻是一类光合放氧的原核生物,除利用二氧化碳作为碳源外,约有一半种类还能吸收利用碳水化合物。具有异养生活能力和遗传操作系统的蓝藻常被用于进行光合作用和光合膜的发生等研究中。其中,集胞藻(Synechocystis sp.) PCC6803已成为研究光合系统的模式种,但是该种不能进行转座诱变。随着蓝藻分子遗传学的不断发展,有希望通过遗传改造的方法获得在完全黑暗条件下异养生长且具有高效基因转移和转座子诱变系统的蓝藻

    表达lac和glk基因的蓝藻的异养生长

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    将来自Brucella abortu的葡萄糖激酶基因glk和大肠杆菌乳糖操纵子基因lacZYA装置于蓝藻glnA启动子下游,以广宿主质粒导入3种蓝藻。基因的表达通过β-半乳糖苷酶和葡糖激酶活性测定得到确证。基因工程藻株均获得了利用乳糖异养生长的能力。集胞藻6803转基因株可在光照加DCMU的条件下利用乳糖生长,而鱼腥藻转基因株依赖乳糖可在完全黑暗条件下生长,并可在黑暗或加DCMU条件下诱导产生异形胞

    一种河道疏浚淤泥复合固化剂

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    本发明公开了一种河道疏浚淤泥复合固化剂。它包括粉剂和液剂:所述的粉剂,按总质量分数100%计,由下列组份组成:水泥15%~25%、火山灰质材料45%~65%、碱类激发剂10%~20%和盐类激发剂5%~10%;所述的液剂,按总质量分数100%计,由下列组份组成:聚乙烯醇2%~7%、聚丙烯酰胺2%~7%、三聚氰胺磺酸盐甲醛缩合物0.5%~1%、余量为水。疏浚淤泥经本发明的河道淤泥复合固化剂固化后,其抗压强度高,能达到筑路要求
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