红树林共生真菌Paecilomyces sp· Tree 1-7代谢产物的研究

用硅胶柱色谱及重结晶等方法,并根据理化性质和光谱数据从一株红树林共生真菌Paecilomycessp.Tree 1-7中分离鉴定了四个化合物,分别为secalon ic ac id A(1),tenellic ac id A(2),大黄素(3),大黄酚(4)。并首次对化合物1和2进行了抗人的肝癌细胞hepG2的活性测试。结果显示,化合物1有很强的抗肿瘤活性,IC50=2.0μg/m l,化合物2的抗肿瘤活性IC50=62.1μg/m l

400 Gbit/s PAM4 CFP8光收发模块技术研究

针对日益增长的以太网带宽需求,提出一种四阶脉冲幅度调制（PAM4）的400Gbit/s外形封装可插拔（CFP8）光收发模块的解决方案。阐述了400Gbit/s标准主要测试指标、技术方案、CFP8的基本原理和结构框图,通过直接调制激光器和电吸收调制激光器两种方案对比,测试得到了优良的光眼图和电眼图,表明PAM4CFP8光模块是实现400Gbit/s以太网传输的良好解决方案,在高速光通信系统中具有广阔的应用前景

New Anthraquinone Derivative Produced by Cultivation of Mangrove Endophytic Fungus Fusarium sp. ZZF60 from the South China Sea

通过人工发酵培养,从南海红树林内源真菌Fusarium sp.ZZF60的培养液中分离得到:1种新蒽醌衍生物,6,8-二甲氧基-1-甲基-2-(3-氧丁基)蒽醌(1),以及5种已知化合物:7-羟基-3-(4-甲氧基苯基)苯并-γ-吡喃酮(2),2,4-二羟基-6-[(1′E,3′E)-1′,3′-戊二烯基]苯甲醛(3),(E)-4-羟基肉桂酸甲酯(4),4-(4-羟基苯基)-2-丁醇(5),4-羟基苯甲酸(6)。它们的结构通过MS、NMR等波谱分析推导确定。初步药理活性显示,化合物1抑制体外培养人喉癌细胞Hep2和人肝癌细胞HepG2的IC50分别为16和23μmol/L。 A new anthraquinone derivative,6,8-dimethoxy-1-methyl-2-（3-oxobutyl） anthrakunthone（1）,together with five known compounds,7-hydroxy-3-（4-methoxyphenyl）-chromen-4-one（2）,2,4-dihydroxy-6-[（1′E,3′E）-penta-1′,3′-dienyl]-benzaldehyde（3）,（E）-4hydroxycinnamic acid methyl ester（4）,4-（4-hydroxyphenyl）-butan2-ol（5）,and 4-hydroxybenzoic acid（6）,were isolated from the marine mangrove endophytic fungus Fusarium sp.ZZF60 from the South China Sea.Their structures were elucidated by spectral data of IR,MS and NMR.In the preliminary bioassay,compound 1 showed cytotoxicity towards Hep2 and HepG2 with IC_50 values of 16 μmol/L and 23 μmol/L,respectively.国家“八六三”计划(2006AA09Z422);国家自然科学基金(20072058和20572136);广西自然科学基金(0832081);广西民族大学科学研究基金(200702YJ18)资助项

Alkaloid Metabolites of Mangrove Endophytic Fungus ZZF42 from the South China Sea

从南海红树林内生真菌ZZF42的代谢产物中分离得到4种生物碱:Apicidin(1),N-methylharman(2),环苯丙-酪肽(3),吲哚-3-乙酸(4)。它们的结构通过波谱方法得到确定。化合物1为首次从该海洋真菌中分离发现,化合物2为首次从该海洋微生物中分离发现。药理实验结果表明,化合物1对人鼻咽癌细胞株KB和人鼻咽癌细胞耐药株KBv200的IC50均小于0.78μg/ml。Four compounds, apicidin（ 1 ） , N-methylharman（ 2 ） , cylo （Phe-Tyr） （ 3 ） and indole-3-acetic acid （4） were isolated from the mangrove endophytic fungus ZZF42 from the South China Sea. Their structures were elucidated by spectral data. Compound 1 was first isolated from the marine fungus. Compound 2 was firstly isolated from marine microorganism. Compound 1 exhibited selective in vitro cytotoxicity towards KB and KBv200 with ICs0 values of less than 0. 78μg/ml.国家863科技攻关计划(2001AA624010);国家自然科学基金(20072058);广东省自然科学重点基金(021732

A New Chromone Derivative Isolated from the Marine Mangrove Endophytic Fungus ZZF41

1个新的苯并-γ-吡喃酮衍生物,3,5-二羟基-2-甲基-6-(3-甲基-2-丁烯基)苯并-γ-吡喃酮(1),以及1个结构独特的环四肽(2)和3个环二肽,从南海红树林内生真菌ZZF41代谢产物中分离得到。它们的结构通过MS、NMR等波谱分析推导确定。化合物1对人鼻咽癌细胞株KB和人鼻咽癌细胞耐药株KBV200显示了微弱的细胞毒活性(IC50均大于50μg/mL),而2对同样细胞株有细胞毒活性(IC50均小于3.56μg/mL)。A new chromone derivative named as 5-dihydroxy-2-methyl-6- （3-methylbut-2- enyl） -4H- chromen-4-one （1）, a novel cyclic tetrapeptide （2） and three cyclic dipeptides were isolated from the mangrove endophytie fungus ZZF41 from the South China Sea. Their structures were characterized by MS, NMR. Compound 1 exhibited weak cytotoxicity towards KB and KBv200 with ICs0values of more 50 μg/mL. Compound 2 showed cytotoxicity towards KB and KBv200 with IC50 values of less 3.56 μg/mL, respectively.国家863计划资助项目(2007AA09Z448);国家自然科学基金资助项目(20772162,20972197);广西自然科学基金资助项目(0832081);广西民族大学科学研究基金资助项目(200702YJ18

Metabolites of Mangrove Endophytic Fungus ZSU-H19 from South China Sea

对南海红树林内生真菌ZSU-H19代谢产物进行研究,从菌体中分离得到6个化合物,现代谱学方法分析表明它们是:ophioglonin(1)、ophioglonin-7-O-β-D-glucopyranoside(2)、吲哚-3-乙酸(3)、尿囊素(4)、环(酪氨酰-亮氨酸)(5)、环(色氨酰-丙氨酸)(6),其中1和2为首次从微生物中分离发现。初步药理活性显示,1和2对人鼻咽癌细胞株KB显示微弱抑制活性,IC50分别为3.0×10-2和1.0×10-2g/L。国家自然科学基金资助项目(20072058,20572136);广西自然科学基金资助项目(0832081);广西民族大学科学研究基金资助项

Studies on the Secondary Metabolites of a Marine Mangrove Fungus Paecilomyces sp. Tree 1-7

Paecilomyces sp.Tree 1-7是一株采自台湾海峡的红树林共生真菌。用硅胶柱色谱及重结晶等方法,并根据理化性质和光谱数据对该菌的代谢产物进行了研究,从菌丝体中分离鉴定出三个化合物,分别为alatern in(1),5,8-二羟基-4-甲基-香豆素(2),3-羟基-3,6-二甲基-2,5-哌嗪(3)。对1进行了抗人的肝癌细胞hepG2及抑制乙酰胆碱酯酶(AChE)的活性测试,结果显示,化合物1有较强的抗肿瘤活性(IC50=7.0μg/mL),及很好地抑制AChE活性(IC50=0.87μg/mL)。The metatrophic fungus Paecilomyces sp.(Tree1-7) was isolated from an estuarine mangrove from the Taiwan Strait.Alaternin(1),5,8-dihydroxy-4-methyl-coumarin(2) and 3-hydroxy-3,6-dimethyl-2,5-piperazine(3) were confirmed by spectroscopic experiments.It was found that alaternin(1) exhibited strong antitumor activity(IC50=7.0 μg/mL),and good in vitro activity against acetylcholineesterase(AChE)(IC50=0.87 μg/mL) firstly.国家“863”基金资助项目(2003AA624010);; 国家自然科学基金资助项目(20572136);; 广东省自然科学基金资助项目(980317