艾纳香油通过NF-κB非经典信号影响花生四烯酸代谢缓解LPS所致巨噬细胞的炎症反应

目的本文旨在探讨艾纳香油（BBO）通过核转录因子κB（NF-κB）非经典通路影响花生四烯酸（AA）代谢通路发挥抗炎作用。方法采用豚鼠离体回肠法检测BBO对慢反应物质（SRS-A）生成的影响，ELISA法检测BBO对脂多糖（LPS） 诱导巨噬细胞前列腺素E2（PGE2）及白三烯 B4（LTB4）产生的影响，qRT-PCR检测BBO对LPS诱导巨噬细胞COX-2、5-LOX、FLAP和RelB表达的影响，Western blot检测BBO对NF-κB非经典通路蛋白肿瘤坏死因子受体作用因子3（TRAF3）、肿瘤坏死因子受体作用因子2（TRAF2）、NF-κB 诱导激酶（NIK）、p100和RelB浓度的影响。结果在1mg·mL-1的BBO药物浓度下减弱了豚鼠回肠的收缩张力（P＜0.001），对SRS-A的生成抑制率达到65.34%；与LPS模型组相比，BBO在40-80 μg· mL-1浓度下降低AA代谢通路中PGE2（P<0.05）和LTB4（P<0.05）的浓度，降低COX-2（P<0.05）、5-LOX（P<0.05）和FLAP（P<0.05）的表达；此外，40-80 μg· mL-1浓度下BBO还降低LPS导致的NF-κB非经典通路中TRAF3（P<0.05）、TRAF2（P<0.05）和NIK（P<0.05）的浓度，进一步抑制p100蛋白的磷酸化（P<0.05），同时抑制转录因子RelB的表达（P<0.05）和RelB蛋白的水平（P<0.05），而BBO自身不引起这些基因与蛋白的变化。结论BBO可能通过抑制NF-κB非经典信号中调节蛋白TRAF3和TRAF2，转录因子RelB的浓度，造成NIK蛋白的诱导激酶作用受到抑制，进一步抑制了p100蛋白的磷酸化及其与转录因子RelB的结合，从而影响到下游AA通路中重要限速酶COX-2、5-LOX和 FLAP的表达与炎症介质PGE2和LTB4的水平发挥抗炎作用

干扰素调节因子5下调PARP-1抑制鼻咽癌细胞的侵袭力

【目的】探讨干扰素调节因子5（IFR5）是否通过下调DNA修复酶PARP-1，进而抑制鼻咽癌细胞的侵 袭能力。【方法】本研究使用的临床标本是经病理确诊的鼻咽癌患者组织标本46例和正常组织51例。免疫组化 检测鼻咽癌组织和正常组织中IRF5和PARP-1的表达。构建IFR5过表达质粒，上调鼻咽癌细胞CNE-2中IFR5 表达，荧光定量PCR（QPCR）和免疫印迹确定建立IRF5过表达细胞。上调IRF5表达后，划痕和transwell实验检 测细胞侵袭能力的改变，QPCR 和免疫印迹检测PARP-1表达量的变化。上调IFR5表达的同时加入PARP-1过 表达质粒，观察PARP-1是否可逆转IFR5对侵袭能力的影响。【结果】免疫组化结果表明IRF5在癌组织中的表达 量低于正常组织，而PARP-1则相反。QPCR和免疫印迹确定建立IRF5 mRNA和蛋白过表达的细胞株CNE-2/ IFR5。细胞划痕实验显示CNE-2/IFR5愈合率较对照组降低（P<0.01），transwell实验表明CNE-2/IFR5细胞穿过 基底膜的数目小于对照组（P<0.01），提示上调 IFR5 可抑制鼻咽癌细胞的侵袭能力。上调 IFR5 后，PARP-1 mRNA和蛋白的表达量降低（P<0.01）；上调IFR5表达的同时过表达PARP-1，可逆转单独上调IRF5对细胞侵袭 能力的改变。提示 IFR5 可通过下调 PARP-1 进而抑制鼻咽癌癌细胞的侵袭能力。【结论】IFR5 可通过下调 PARP-1进而抑制鼻咽癌癌细胞的侵袭能力。本研究可能为降低鼻咽癌侵袭能力提供新的靶点

Physical health care monitoring for people with serious mental illness

Background Current guidance suggests that we should monitor the physical health of people with serious mental illness and there has been a significant financial investment over recent years to provide this. Objectives To assess the effectiveness of physical health monitoring as a means of reducing morbidity, mortality and reduction in quality of life in people with serious mental illness. Search methods We searched the Cochrane Schizophrenia Group Trials Register (October 2009) which is based on regular searches of CINAHL, EMBASE, MEDLINE and PsycINFO. Selection criteria All randomised or quasi-randomised clinical trials focusing on physical health monitoring versus standard care or comparing i) self monitoring vs monitoring by health care professional; ii) simple vs complex monitoring; iii) specific vs non-specific checks iv) once only vs regular checks or v) comparison of different guidance. Data collection and analysis The authors (GT, AC, SM) independently screened search results and identified three studies as possibly fulfilling the review's criteria. On examination, however, all three were subsequently excluded. Main results We did not identify any randomised trials which assessed the effectiveness of physical health monitoring in people with serious mental illness. Authors' conclusions There is no evidence from randomised trials to support current guidance and practice. Guidance and practice are based on expert consensus, clinical experience and good intentions rather than high quality evidenc

CMOS集成电路用Φ150-200mm外延硅材料

报道了Φ150mm CMOS硅外延材料的研究开发及集成电路应用成果，对Φ200mmP/P~-硅外延材料进行了初步探索研究。Φ150mm P/P~+硅外延片实现了批量生产，并成功应用于集成电路生产线，芯片成品率大于80%。硅外延片的参数指标能满足集成电路制造要求

Application of team tutorial system in the training of undergraduate nursing students (导师团队制在护理本科生培养中的应用研究)

Objective To evaluate the application effect of the team tutorial system in the training of undergraduate nursing students. Methods Totally 230 undergraduate nursing students form 4 classes of academic year 2019 were selected as the research objects, and they were divided into observation group (n=117)and control group (n=113) by using the cluster random sampling method. The control group adopted the routine education method in module teaching, and the observation group adopted the team tutorial system. A 1- academic year intervention was carried out, and the comprehensive test outcomes, humanistic quality, communication skills, attitudes and professional identity of the two groups were evaluated at the end of the semester. Results The scores of comprehensive test, humanistic quality assessment and communication skills assessment in the observation group were higher than those of the control group, and the difference was statistically significant (P<0. 01). The score of attitudes and professional identity scale in the observation group was higher than that of the control group, and the difference was statistically significant (P<0. 01). Conclusion The team tutorial system is conducive to improving the comprehensive performance, humanistic quality, communication skills, attitude and professional identity of undergraduate nursing students, and improving the comprehensive ability of nursing students. (目的 评价导师团队制在护理本科生培养中的应用效果。方法 选取2019级4个班共230名护理本科生作为研究对象, 采用整群随机抽样分为观察组(n=117)和对照组(n=113)。观察组采用导师团队制; 对照组采用传统教育法。干预1个学年, 学期末对两组护生综合成绩、人文素质、沟通技能态度及其专业认同感进行评价。结果 观察组护生综合成绩、人文素质、沟通技能态度得分均高于对照组, 差异有统计学意义(P＜0. 01); 观察组护生专业认同得分高于对照组, 差异有统计学意义(P＜0. 01)。结论 导师团队制有利于提高护生综合成绩、人文素质、沟通技能态度及其专业认同感, 提高护生的综合能力。

气脉冲除灰装置

气脉冲除灰装置包括混合器(1)和与之相连接的高效点火器(2)，通过管道(3)与燃烧室(4)相连，后边接的是输出管(5)，整个连接方式都是用法兰连接或焊接在燃烧室的内侧焊接有扰动器件，它使燃烧加速，并产生较强的气脉冲，并伴随有较大声响的声输出。在气脉冲的动能和声振动的反复循环作用下，达到清除空气预热器内积灰之目的

水煤浆直流型低压雾化喷嘴

一种单孔直流型低压雾化喷嘴，由喷嘴、外套和输浆管组成，在喷嘴的周圈有用于注入空气射流的排孔。当煤浆射流由输浆管输入后，经高速气流多次雾化形成雾炬，达到利于燃烧的目的。特点是煤浆通道直径大不易堵塞，低压运行磨损小、用碳钢材料就可制造，降低了喷嘴成本，结构简单，雾化效果好，运行稳定、操作和维修方便，调节范围大，出力可由每小时几百公斤到每小时三吨