Cross testing instrument of nucleic acid amplification genechips

为了提高基因芯片技术的推广应用,研制了一种集PCR扩增、杂交、清洗和检测分析于一体的新型仪器;可以实现快速PCR,30个扩增循环可以在15min内完成。杂交及冲洗在计算机控制下自动完成,避免了人工干预以及环境变化所造成的误差。检测部分采用汞弧灯作为激发光源,CCD采集荧光信号,配合信号识别分析软件自动识别基因芯片信息

纤维连接素对髁突软骨细胞在Cytodex-3微载体表面贴附、铺展及增殖的影响

目的 :研究纤维连接素 (FN)对下颌骨髁突软骨 (MCC)细胞在Cytodex 3微载体表面生长的影响。方法 :采用机械分离及酶消化法获得MCC细胞 ,将细胞以 1× 10 8/L密度分别接种于含有空白及FN(2 0mg/L)包埋的微载体的自制转瓶细胞培养系统 ,利用环境扫描电镜对培养MCC细胞在两组微载体表面的生长方式进行动态观察。结果 :MCC细胞可快速贴附于FN包埋的Cytodex 3微载体表面 ,铺展速度快于空白微载体组 ,细胞形态不规则 ,更为扁平 ,有较多伪足。细胞在包埋微载体表面生长速度显著快于空白微载体组 ,细胞指数生长期提前 ,细胞倍增时间缩短 ,最终生长密度高于对照组。结论 :细胞在微载体表面的生长有赖于适当的细胞外基质成分 ,FN可促进MCC细胞在微载体表面的贴附及增殖 ,可用于MCC细胞大规模扩增的微载体表面处理

髁突软骨细胞分离及体外快速扩增

目的 探索在短期内获得大量成活率高、分化良好的下颌髁突软骨（ｍａｎｄｉｂｕｌａｒ ｃｏｎｄｙｌａｒ ｃａｒｔｉｌａｇｅ，ＭＣＣ）方法 采用细胞工程技术进行兔ＭＣＣ细胞的Ｃｙｔｏｄｅｘ－３微载体培养，应用先进的环境挪描电镜技术对培养ＭＣＣ细胞在微载体地面的生长方式进行了动态观察。结果 ＭＣＣ细胞可快速贴附于Ｃｙｔｏｄｅｘ－３微载体表面，但其 展相对较慢。细胞 展后生长加速，到培养后期，细胞密度可达

髁突软骨细胞分离及体外快速扩增

目的 探索在短期内获得大量成活率高、分化良好的下颌髁突软骨（ｍａｎｄｉｂｕｌａｒ ｃｏｎｄｙｌａｒ ｃａｒｔｉｌａｇｅ，ＭＣＣ）方法 采用细胞工程技术进行兔ＭＣＣ细胞的Ｃｙｔｏｄｅｘ－３微载体培养，应用先进的环境挪描电镜技术对培养ＭＣＣ细胞在微载体地面的生长方式进行了动态观察。结果 ＭＣＣ细胞可快速贴附于Ｃｙｔｏｄｅｘ－３微载体表面，但其 展相对较慢。细胞 展后生长加速，到培养后期，细胞密度可达

纤维连接素对髁突软骨细胞在Cytodex-3微载体表面贴附、铺展及增殖的影响

目的 :研究纤维连接素 (FN)对下颌骨髁突软骨 (MCC)细胞在Cytodex 3微载体表面生长的影响。方法 :采用机械分离及酶消化法获得MCC细胞 ,将细胞以 1× 10 8/L密度分别接种于含有空白及FN(2 0mg/L)包埋的微载体的自制转瓶细胞培养系统 ,利用环境扫描电镜对培养MCC细胞在两组微载体表面的生长方式进行动态观察。结果 :MCC细胞可快速贴附于FN包埋的Cytodex 3微载体表面 ,铺展速度快于空白微载体组 ,细胞形态不规则 ,更为扁平 ,有较多伪足。细胞在包埋微载体表面生长速度显著快于空白微载体组 ,细胞指数生长期提前 ,细胞倍增时间缩短 ,最终生长密度高于对照组。结论 :细胞在微载体表面的生长有赖于适当的细胞外基质成分 ,FN可促进MCC细胞在微载体表面的贴附及增殖 ,可用于MCC细胞大规模扩增的微载体表面处理

Design of coaxial intervention catheter robot for Endoscopic Retrograde Cholangio-Pancreatography

Due to the high incidence of biliary and pancreatic diseases, ERCP (Endoscopic Retrograde Cholangio-Pancreatography) is widely used as an effective method for treating biliary and pancreatic diseases. However, the location of biliary and pancreatic diseases is special, and the currently used ERCP surgical instruments are not flexible enough to make the intubation process difficult and cause damage to human tissues. This paper presents a new type of coaxial catheter intervention robot, which is composed of a flexible mechanical arm, a driving mechanism, and a guide wire conveying mechanism. The robot is coaxially transported to the nipple through the duodenoscope, which can provide the doctor with a clear surgical field of vision. The 2-degree-of-freedom flexible manipulator can bend in four directions, so that the direction of the catheter is as consistent as possible with the axis of the cholangiopancreatic duct, thus simplifying insertion Tube process. After the prototype was manufactured, the biliary and pancreatic duct model intervention experiment was carried out. The experiment verified the feasibility of the flexible robot system. The experimental results showed that compared with manual intubation, the efficiency is higher and the operation difficulty is lower

萌育对芝麻籽粒含氮物质的影响Effect of germination on nitrogen-containing substances in sesame seeds

为探究芝麻籽粒萌育过程中含氮物质的变化，采用去离子水、0.5%氯化钙溶液、1%氯化钙溶液对芝麻籽粒浸泡30 min，然后在30 ℃下进行萌育处理，测定芝麻籽粒萌育0～72 h 期间粗蛋白质、可溶性蛋白质、游离氨基酸、蛋白质水解度以及蛋白质分子质量的变化。结果表明：萌育过程中，粗蛋白质含量先减少后增加再减少；可溶性蛋白质含量在0～4 h略微增加，随后下降，72 h时降低了约25%，3种浸种处理方式变化趋势一致；游离氨基酸含量和蛋白质水解度逐渐增大，3种浸种处理方式游离氨基酸含量有显著性差异；SDS-PAGE结果显示，50～95 kDa蛋白质亚基的含量减少，11S蛋白的含量呈波动性变化，最终呈增加趋势，17 kDa蛋白质亚基的含量增加，2S蛋白的含量明显减少。研究说明萌育可以显著改变芝麻籽粒中含氮物质的含量，大分子蛋白质降解为小分子蛋白质，同时2S等小分子蛋白质也发生了降解。 In order to explore the changes of nitrogen-containing substances during sesame seed germination, sesame seeds were soaked respectively in deionized water, 0.5% calcium chloride solution and 1% calcium chloride solution for 30 min and then germinated at 30 ℃. The changes of crude protein, soluble protein, free amino acids, degree of hydrolysis of protein and molecular weight of protein of sesame seed were measured during 0-72 h germination. The results showed that the content of crude protein decreased first, then increased and then decreased. The soluble protein content increased slightly at 0-4 h, then decreased, and decreased by about 25% at 72 h, and variation trend of three soaking methods was consistent.The content of free amino acids and the degree of hydrolysis of protein increased gradually, and there was significant difference in the content of free amino acids among the three soaking treatments. The results of SDS-PAGE showed that the content of 50-95 kDa protein subunit decreased, the content of 11S protein fluctuated during germination and finally increased, the content of 17 kDa protein subunit increased, and the content of 2S protein decreased significantly. The germination can significantly change the nitrogen content in sesame seeds, macromolecular proteins are degraded into small molecular proteins, and small molecular proteins such as 2S are also degraded

基于叶绿体InDel标记对玉米杂交种正反交的鉴定

玉米的正反交杂交种在有些表型性状上存在差异,在有些品种中甚至直接影响总体产量。为了避免之前的鉴定正反交方法在取样类型和时间上存在的一定的局限性,本研究以玉米叶绿体基因组Insertion/Deletion(In Del)标记为基础开发引物,使用玉米叶片材料进行检测。结果显示CPMIDP01和CPMIDP03两个位点可分别鉴定玉米杂交种郑单958和京科968的正反交组合,并可组合成效果稳定的二重PCR,从而得到一种对玉米正反交种的快速鉴定方法。此方法可应用于中国主要玉米品种正反交鉴定中,作为核基因组检测的补充,进一步完善品种真实性及品系溯源信息

2002–2010年中国典型生态系统辐射及光能利用效率数据集

辐射是陆地生态系统能量的主要来源,其利用效率表现为光能利用率,反映了生态系统转化光能、生成有机物质的能力。揭示典型生态系统的辐射及光能利用效率可以为评估区域光能资源及其利用效率提供参考,也为评估区域有机物质固定能力及碳吸收能力提供依据。基于中国陆地生态系统通量观测研究联盟(China FLUX)的长期观测结果及已发表文献的公开数据,构建了2002–2010年中国典型生态系统辐射及光能利用效率数据集,包含51个生态系统126个站点年辐射、光能利用效率及吸收光能利用效率的观测记录。另外,本数据集还包含生态系统代码、年份、经度、纬度、海拔、生态系统类型、年均气温、年总降水量、年均CO2质量浓度、年均叶面积指数、最大叶面积指数等生物气候信息。本数据集可以为评估生态系统生产能力、应对气候变化等方面的研究提供数据支持