Effect of intermittent Pringle maneuver on perioperative outcomes and long-term survival following liver resection in patients with hepatocellular carcinoma: a meta-analysis and systemic review

Abstract Background Intermittent Pringle maneuver (IPM) is commonly used to control bleeding during liver resection. IPM can cause ischemia–reperfusion injury, which may affect the prognosis of patients with hepatocellular carcinoma (HCC). The present meta-analysis was conducted to evaluate the effect of IPM use on perioperative outcomes and long-term survival in patients with HCC. Methods A systemic literature search was performed in the PubMed, Embase, Web of Science, and Cochrane Library databases to identify randomized controlled trials and retrospective studies that compared the effect of IPM with no Pringle maneuver during liver resection in patients with HCC. Hazard ratio (HR), risk ratio, standardized mean difference, and their 95% confidence interval (CI) values were calculated based on the type of variables. Results This meta-analysis included nine studies comprising one RCT and eight retrospective studies and involved a total of 3268 patients. Perioperative outcomes, including operation time, complications, and length of hospital stay, except for blood loss, were comparable between the two groups. After removing the studies that led to heterogeneity, the results showed that IPM was effective in reducing blood loss. Five studies reported overall survival (OS) and disease-free survival (DFS) data and eight studies reported perioperative outcomes. No significant difference in OS and DFS was observed between the two groups (OS: HR, 1.01; 95% CI, 0.85–1.20; p = 0.95; DFS: HR, 1.01; 95% CI, 0.88–1.17; p = 0.86). Conclusion IPM is a useful technique to control blood loss during liver resection and does not affect the long-term survival of patients with HCC

Equilibrium Relationship between Ecosystem Service Supply and Consumption Driven by Economic Development and Ecological Restoration

Economic development and ecological restoration guided by policies has had a profound impact on the relationship between ecosystem service supply and consumption in the past few decades. Exploring the relationship in specific study areas is necessary to support regional planning for sustainability. In this study, we analyzed the equilibrium relationship between ecosystem service supply and consumption driven by economic development and ecological restoration from 1995 to 2018 in Northeast China. We quantified the supply and consumption of three critical ecosystem services, food production, water yield and atmospheric regulation, and identified the main areas for supply and consumption of ecosystem services. We measured the quantity and spatial equilibrium relationship between ecosystem service supply and consumption driven by economic development and ecological restoration at economic megaregion scale. We found the new industrial structure shaped by regional economic development policies formed the new ecosystem service consumer demand. The development of agricultural industry not only resulted in the increase of water consumption, but also the scope of water consumption was expanded in Northeast China. The development of tertiary industry causes the change of atmospheric regulation consumption in urban agglomeration. What’s more, economic development exacerbated imbalances in the supply and consumption of ecosystem services. The unbalanced areas where food supply was less than consumption were in the clusters of Harbin, Changchun, Shenyang, and coastal cities. Since food products can circulate freely through the market, this imbalance will not fundamentally affect regional food security, but will improve the level of agricultural industrialization and scale. The implementation of the grain economic policy caused the rapid expansion of unbalanced areas where water supply was less than consumption. The incredible space mismatch between the supply and consumption of ecosystem services still exists for a long time. However, the implementation of the ecological restoration didn’t effectively solve the problem of insatiable ecosystem service consumption. The ecosystem service consumption brought by economic development need to be given the utmost attention when formulating ecological restoration policies and economic development policies. The results can provide insights for enhancing economic sustainability in northeast china, as well other economic megaregion with similar characteristics throughout the world

Additional file 1 of Comparison of liver resection and radiofrequency ablation in long-term survival among patients with early-stage hepatocellular carcinoma: a meta-analysis of randomized trials and high-quality propensity score-matched studies

Additional file 1 PRISMA checklis

Additional file 2 of Comparison of liver resection and radiofrequency ablation in long-term survival among patients with early-stage hepatocellular carcinoma: a meta-analysis of randomized trials and high-quality propensity score-matched studies

Additional file 2 Supplementary material: Supplementary material S1: Search strategy. Supplementary material S2 NOS score for PSM studies. Supplementary material S3 Risk bias of RCTs. Supplementary material S4 1-,3-,and 5-year survival rate, disease-free survival rate, and recurrence rate. Supplementary material S5 Forest plot for sensitivity analysis of overall survival and disease-free survival. Supplementary material S6 Funnel plot for overall survival and disease-free survival. Supplementary material S7 Meta-regression. OS, overall survival; DFS, disease-free survival; RFA, radiofrequency ablation. Supplementary material S8 Subgroup analysis for OS and DFS based on modality of RF

Genetic programs in human and mouse early embryos revealed by single-cell RNA sequencing.

Mammalian pre-implantation development is a complex process involving dramatic changes in the transcriptional architecture. We report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos, using single-cell RNA sequencing. Based on single-nucleotide variants in human blastomere messenger RNAs and paternal-specific single-nucleotide polymorphisms, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25 to 53%). By weighted gene co-expression network analysis, we find that each developmental stage can be delineated concisely by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation and metabolism, acting in a step-wise fashion from cleavage to morula. Cross-species comparisons with mouse pre-implantation embryos reveal that the majority of human stage-specific modules (7 out of 9) are notably preserved, but developmental specificity and timing differ between human and mouse. Furthermore, we identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely to be key in driving mammalian pre-implantation development. Together, the results provide a valuable resource to dissect gene regulatory mechanisms underlying progressive development of early mammalian embryos

Genetic programs in human and mouse early embryos revealed by single-cell RNA sequencing.

Mammalian pre-implantation development is a complex process involving dramatic changes in the transcriptional architecture. We report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos, using single-cell RNA sequencing. Based on single-nucleotide variants in human blastomere messenger RNAs and paternal-specific single-nucleotide polymorphisms, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25 to 53%). By weighted gene co-expression network analysis, we find that each developmental stage can be delineated concisely by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation and metabolism, acting in a step-wise fashion from cleavage to morula. Cross-species comparisons with mouse pre-implantation embryos reveal that the majority of human stage-specific modules (7 out of 9) are notably preserved, but developmental specificity and timing differ between human and mouse. Furthermore, we identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely to be key in driving mammalian pre-implantation development. Together, the results provide a valuable resource to dissect gene regulatory mechanisms underlying progressive development of early mammalian embryos

Identification of Transcriptome-Derived Microsatellite Markers and Their Association with the Growth Performance of the Mud Crab (<i>Scylla paramamosain</i>)

<div><p>Microsatellite markers from a transcriptome sequence library were initially isolated, and their genetic variation was characterized in a wild population of the mud crab (<i>Scylla paramamosain</i>). We then tested the association between these microsatellite markers and the growth performance of <i>S. paramamosain</i>. A total of 129 polymorphic microsatellite markers were identified, with an observed heterozygosity ranging from 0.19 to 1.00 per locus, an expected heterozygosity ranging from 0.23 to 0.96 per locus, and a polymorphism information content (PIC) ranging from 0.21 to 0.95 per locus. Of these microsatellite markers, 30 showed polymorphism in 96 full-sib individuals of a first generation family. Statistical analysis indicated that three microsatellite markers were significantly associated with 12 growth traits of <i>S. paramamosain</i>. Of these three markers, locus Scpa36 was significantly associated with eight growth traits, namely, carapace length, abdomen width (AW), body height (BH), fixed finger length of the claw, fixed finger width of the claw, fixed finger height of the claw, meropodite length of pereiopod 2, and meropodite length of pereiopod 3 (MLP3) (<i>P</i><0.05). Locus Scpa75 was significantly associated with five growth traits, namely, internal carapace width, AW, carapace width at spine 8, distance between lateral spine 2 (DLS2), and MLP3 (<i>P</i><0.05). Locus Spm30 was significantly associated with BH, DLS2, and body weight (<i>P</i><0.05). Further analysis suggested a set of genotypes (BC at Scpa36, BC and BD at Scpa75, and AC at Spm30) that have great potential in the selection of <i>S. paramamosain</i> for growth traits. These findings will facilitate the development of population conservation genetics and molecular marker-assisted selective breeding of <i>S. paramamosain</i> and other closely related species.</p></div