Silicon effects on phenols metabolism of Avicennia marina under cadmium stress

采用水培方式研究硅对重金属镉胁迫下白骨壤幼苗酚类代谢影响,探讨硅对白骨壤耐受重金属胁迫的影响,结果表明,低浓度镉胁迫(Cd 0.5 Mg·l-1)下,随着外源硅的增加,叶片和根系丙二醛含量降低,但SI 50 Mg·l-1处理中各器官总酚含量最高,硅对各器官单宁含量影响不显著;高浓度镉(Cd 5 Mg·l-1)导致白骨壤叶片和根系膜脂过氧化,使得丙二醛含量显著高于对照,外源硅的添加显著降低了白骨壤叶片和根系丙二醛积累,SI 100Mg·l-1显著增加了各器官中总酚含量和单宁含量,根系丙二醛含量与根系总酚含量呈显著负相关,叶片丙二醛含量与叶片单宁含量呈显著负相关,表明硅显著刺激高镉胁迫下白骨壤植物组织中酚类物质代谢,增加根系和叶片的抗氧化性,进而增加白骨壤对镉的耐受性。The effects of silicon on the phenols metabolism in Avicennia marina seedlings were studied under the stress of cadmium by adopting the hydroponic method,and the effects of silicon on the resistance of A.marina seedling to cadmium were further explored.The results showed that under the stress of cadmium of low concentration( Cd 0.5 mg·L-1),the content of malondialdehyde in the leaves and roots decreased with the increase of the exogenous silicon level,and the total phenol content in each organ was the highest at Si 50 mg·L-1,while the effect of silicon on the tannin content in each organ was not significant.The high-concentration cadmium( Cd 5 mg·L-1) led to the membrane lipid peroxidation of the leaves and roots in A.marina,making the content of malondialdehyde significantly higher than that of the control; the addition of the exogenous silicon significantly reduced the accumulation of malondialdehyde in the leaves and roots of A.marina,and Si 100 mg·L-1significantly increased the contents of total phenol and tannin in each organ.Significant negative correlations between the malondialdehyde content and total phenol content in roots and between the malondialdehyde content and tannin content in leaves were found,indicating that silicon significantly stimulated phenolic metabolism in A.marina organs under the stress of high-concentration cadmium,increased the oxidation resistance of the root system and leaves,and thus increased the tolerance of A.marina to cadmium.国家自然科学基金项目(31370516;31170471); 闽南师范大学博士科研启动项目(2006L21432); 闽南师范大学园林植物生长发育与生态配置校级创新团队项目资

针刺治疗特发性血小板减少性紫癜并荨麻疹验案1则

特发性血小板减少性紫癜为临床常见自身免疫性出血性疾病,以皮肤黏膜及内脏出血为主要临床表现,且易并发自身免疫系统相关疾病,甚者可引发颅内出血导致死亡,现代医学一般采用糖皮质激素进行治疗,但疗效有限,毒副作用大。本案患者诊断为特发性血小板减少性紫癜2年余,近期

The mechanism study of ATM / NF-kappaB activation in pro-inflammatory cytokine TNF-alpha and IL-6 increased migration abilities in lung cancer cells

【背景和目的】免疫微环境在肺癌的侵袭转移过程中起着重要作用，TNF-α和IL-6作为两种重要的促炎因子，参与了免疫、炎症反应及肿瘤发生发展的全过程，为肿瘤相关炎症的关键调节分子且可通过上调p38、ERK、NF-κB通路诱导MMPs的表达最终促进肿瘤细胞的侵袭和迁移，但这一机制在肺癌中尚不明确。ATM作为DNA损伤信号传导通路中最早的传感和中枢调控基因，可以多种途径激活NF-κB，从而调控下游靶基因的表达。此外，最近研究发现TGF-β1可激活ATM调控乳腺癌细胞的迁移。这些研究表明，ATM除参与DNA损伤修复外，还与肿瘤细胞的转移相关。因此，炎症因子TNF-α、IL-6对肺癌细胞迁移能力的影响及...Inflammation was reported to have close relationship with carcinogenesis and metastasis. As the important proinflammatory cytokines, TNF-alpha and IL-6 are involved in lung cancer development. ATM, which is activated by DNA double break, was found to be activated by the treatment with TNF-alpha and IL-6, but the exact roles of ATM activation in proinflammatory cytokines such as TNF-alpha and IL-6 ...学位：理学硕士院系专业：医学院_微生物学学号：2452011115339

中国石松类和蕨类植物的多样性与地理分布

物种编目及其科属系统排列是了解生物多样性的基础,本文采用Flora of China使用的分类系统,结合最新分子分类学研究成果以及近几年发表的新资料,对中国石松类和蕨类植物多样性和地理分布数据进行了统计和分析。结果表明中国共有石松类和蕨类植物40科178属2,147种5个亚种118个变种,其中特有种839个,占总种数的39.08%。种数最多的5个科依次为鳞毛蕨科(505种,含种下单位,下同)、蹄盖蕨科(323种)、水龙骨科(280种)、凤尾蕨科(266种)和金星蕨科(209种);种数最多的5个属依次为耳蕨属(Polystichum,209种)、鳞毛蕨属(Dryopteris,176种)、蹄盖蕨属(Athyrium,137种)、双盖蕨属(Diplazium,98种)和凤尾蕨属(Pteris,97种)。在地理分布上,种数排名前5的省份为云南(1,365种)、四川(875种)、贵州(838种)、广西(785种)和台湾(779种)。含中国特有石松类和蕨类植物的科属中,排前3位的科分别为鳞毛蕨科(257种)、蹄盖蕨科(169种)和凤尾蕨科(113种);排前3位的属为耳蕨属(140种)、蹄盖蕨属(82种)和鳞毛蕨属(61种)

Dynamics of Phenolic Acids Extracted fromKandelia obovata(S.L.)Yong Leaf Litters and Their Allelopathic Effects on Nitzschia closterium

采用生物测试法,以九龙江口的优势藻种新月菱形藻(nITzSCHIA ClOSTErIuM)为受体研究了秋茄[kAndElIA ObOVATA(S.l.)yOng]凋落叶的抑藻作用,并利用高效液相色谱法对其分解过程中的酚酸化感物质进行了分析.通过酚酸对新月菱形藻密度以及生理生化的影响,探讨了秋茄凋落叶对底栖硅藻的化感效应,丰富了安全、有效防治藻类的爆发的理论依据与实践经验,为揭示红树植物与林内微藻间相互关系提供新的科学依据.对新月菱形藻密度测定的研究发现:未分解秋茄凋落叶水提液的抑藻作用存在浓度效应,随着水提液浓度的增加,抑制作用增强;半分解秋茄凋落叶(半分解期T50=42d)水提液对新月菱形藻的生长则无明显影响.高效液相色谱检测结果确定秋茄凋落叶中存在对羟基苯甲酸、香草酸、丁香酸、绿原酸和咖啡酸;随着凋落叶的分解,酚酸的含量下降.标准酚酸物质及混合酸均对新月菱形藻产生化感抑制作用,且随着浓度增大,抑制作用增强.这表明在红树林根际-沉积物-水环境界面上,红树植物凋落物对微藻的繁殖起到了一定的调控作用,而酚酸是其中起重要作用的一类物质.In order to explore the allelopathy of mangrove leaf litters,Nitzschia closterium was used to examine the allelopathy effects of Kandelia obovata(S.L.)Yong leaf litters on algagrowth.The results showed that water extract of undecomposed K.obovataleaf litters significantly inhibited the growth of N.closterium,the inhibition effects increased at higher concentrations of leaf litters.Water extract of half-decomposed(t50=42d)K.obovataleaf litters had no significant effect on the growth of N.closterium.Hydroxybenzoate,vanilloid,clove acid,chlorogenic acid and caffeic acid were determined by high performance liquid chromatography in K.obovata leaf litters,and the concentration of these components decreased with the decomposition of leaf litters.Our data showed that these phenolic substances had significant allelopathy effect on N.closterium.Therefore,mangrove plants controlled the growth of microalgae in the rhizosphere-sediment-water interface,and allelochemicals was an important reason.This study enriched the theory and practical experience in preventing algal outbreaks safely and effectively,providing a new basis for revealing the relationship between mangroves and microalgae.国家自然科学基金(31170471

Responses of Phenolic Compounds in Kandelia obovata(S.L.)Yong to Cadmium-Pyrene Contamination

以红树植物秋茄[kAndElIA ObOVATA(S.l.)yOng)]为研究试材,通过镉(Cd)和芘(Pyr)交互胁迫盆栽实验,研究秋茄体内酚类化合物对Cd-Pyr复合污染的响应,旨在探讨红树植物通过酚类化合物的调节耐受重金属和PAHS复合污染的作用机制.Cd胁迫含量为0,5,10,20,40Mg/kg,Pyr胁迫含量为2,10,50Mg/kg,二者交互胁迫.结果显示:Cd和Pyr胁迫下秋茄根、茎、叶合成大量的总酚、单宁和缩合单宁,含量均随着胁迫含量增大呈现上升趋势.各组织总酚、单宁和缩合单宁含量均表现出茎>叶>根.高含量Cd-Pyr复合胁迫下根、茎和叶的总酚、单宁和缩合单宁含量显著高于同一水平Cd胁迫组.根和叶中的总酚、单宁及缩合单宁的含量与相同器官中Cd的含量均呈显著正相关,可推测复合污染背景下总酚、单宁和缩合单宁参与了秋茄根和叶对Cd的螯合,增强了秋茄对Cd的耐性.In recent years,with the rapid urbanization and industrialization,many pollutants are discharged into mangrove ecosystems.Heavy metals and polycyclic aromatic hydrocarbons are particularly common in mangrove ecosystems.It is meaningful to study the response of mangrove plants to heavy metals-polycyclic aromatic hydrocarbons,which can evaluate correctly the tolerance mechanisms of mangrove plant under combined pollutions.To explore the tolerance mechanisms of phenolic compounds of mangrove plant under combined contaminations of heavy metals and polycyclic aromatic hydrocarbons,the responses of phenolic compounds in Kandelia obovata(S.L.)Yong under Cd and pyrene(Pyr)combined contamination were studied by apot experiment.The results showed that a large amount of total phenols,tannins,and condensed tannins were generated in the roots,stems,leaves of K.obovata under Cd/Pyr single stress and Cd and Pyr co-stress,and their contents increased with increasing stress.The order of the contents of total phenols,tannins,and condensed tannins in the plant tissues was as follows:stem>leaf>root.Additionally,the contents of total phenols,tannins,and condensed tannins in roots,stems,and leaves were significantly higher under high levels of co-stress than under single Cd-stress at the same level.There were significant positive correlations between the content of total phenol and Cd concentration in the root and leaf,between the content of tannin and Cd concentration in the root and leaf,and between the content of condensed tannin and Cd concentration in the root and leaf.Therefore,it was suggested that total phenols,tannins and condensed tannins may be involved in Cd tolerance in the roots and leaves of K.obovata under Cd-Pyr combined contamination.国家自然科学基金重点项目(2013CB956504);国家自然科学基金(30970527;31170471;31370516

Expressions of Epstein-Barr Virus Latent Membrane Lmpl and P53 Protein in Nasonpharyngeal Carcinoma and their Clinical Significance

目的　探讨 EB病毒潜伏感染和抑癌基因 P5 3异常表达与鼻咽癌的关系。方法　用免疫组化 S- P方法对 5 9例鼻咽癌 (NPC)和 12例鼻咽部正常或慢性炎症组织进行单克隆抗体 L MP1、P5 3标记 ,并结合组织学类型、分化程度、发病年龄进行分组研究。结果　正常和慢性炎症的鼻咽粘膜均呈阴性表达。NPC中 ,L MP1阳性率 6 9.4 9%(4 1/ 5 9) ,P5 3阳性率 4 4 .0 6 % (2 6 / 5 9) ,双阳性率 30 .5 1% (18/ 5 9)。L MP1在非角化性癌、低分化癌及青年组 NPC中高表达 ;在鳞癌、高分化癌及老年组中低表达。P5 3在低分化癌中高表达 ;但与 NPC组织学类型、发病年龄不存在明显相关性。结论　在 NPC发生、发展过程中 EBV潜伏感染起主导作用 ,P5 3基因异常表达可能起协同作用。EB病毒潜伏感染是 NPC发病年龄年轻化的主要原因之一Objective To study the correlation of Epstein Barr Virus Latent infection and suppressor gene P53 mutation with the human nasonpharyngeal Carcinoma(NPC).Methods\ The nasonpharyngeal bioptic tissue of 59 cases of NPC and 12 cases of normal and chronic inflammation were examined to observe the Lmp 1 and P53 protein expression by using immunohistochemistry technique(S P methods).Results\ In 59 cases of NPC,Lmp 1 positive rate is 69 49%,P53 44 06% and both P53 and Lmp 1 30 51%.Statistically positive expression of Lmpl was correlated with hostologica type and differentiation degree of NPC,and age of illness.Statistically positive expression of P53 was correlated with low differentiation tumor.There are no significant differences in positive expression of P53 among various ages and hostological type.Conclusions In the course of tumorigenesis of NPC,Epstein Barr Virus latent infection plays the major role and P53 mutation plays the coordination role.Epstein Barr Virus latent infection is the main course that young people get ill

Effects of Si on the Low Molecular Weight Organic Acid Metabolism of Avicennia marina Under Cd Stress

以红树植物白骨壤为实验材料,通过水培实验,施加不同浓度的SI和Cd,对比研究Cd胁迫下外源SI对白骨壤幼苗低分子质量有机酸代谢的影响,以探讨外源SI对红树植物抗重金属的影响效应和作用机理.结果表明:Cd胁迫显著降低白骨壤幼苗各器官和根系分泌物中低分子质量有机酸含量;SI对有机酸代谢的影响随Cd处理水平不同而不同,在对照(Cd 0Mg/l)和低质量浓度(Cd 0.5Mg/l)处理下,SI对各器官有机酸含量影响不显著或降低其含量,高质量浓度Cd(Cd 5Mg/l)处理下,SI显著增加叶片和茎部有机酸总量,显著增加根系分泌物中柠檬酸含量;白骨壤各器官和根系分泌物有机酸含量两两间显著相关.由此推测SI是白骨壤生长的有益元素,SI缺乏造成的营养胁迫促使植物体内有机酸代谢做出响应;SI促进高质量浓度Cd处理下植物体内的有机酸代谢,对缓解白骨壤Cd毒害效应具有重要意义.Mangrove plants seem to be highly tolerant of high levels of heavy metal pollution.Some researchers have focused on the mechanisms involved in their metal uptake and tolerance.However the important mechanisms involved are still only partially understood.There are higher concentrations of available Si in mangrove sediments.There is increasing evidence that Si has many beneficial effects on mangrove growth and development and can improve heavy metal resistance of mangrove.In order to explore the Si alleviation mechanisms of the toxicity of heavy metal on mangrove,we investigated the effects of Si and Cd on low molecular weight organic acid metabolism of Avicennia marina by pot experiment.The concentration of low molecular weight organic acid in different organs and root exudates were decreased by Cd addition.The effect of Si on low molecular weight organic acid metabolism varied with Cd treatments.Si did not affect or decreased the concentrations of low molecular weight organic acid in different organs and root exudates in 0mg/L and 0.5mg/L Cd treatments.Si increased the total concentrations of low molecular weight organic acid in the stem and leaf and the concentration of citric acid in root exudates under 5mg/L Cd treatments.The correlation analysis showed that there was a significantly positive correlation between the total concentration of low molecular weight organic acid in different organs and root exudates of A.marina.This suggested that Si was beneficial for the healthy growth and development of A.marinaand organic acid metabolism was enhanced by the lack of Si nutrition stress.Si enhanced low molecular weight organic acid metabolism of A.marina under high Cd stress,which may be related to the amelioration by Si of Cd toxicity.国家自然科学基金重点项目(2013CB956504);国家自然科学基金项目(30970527;31170471;31370516