Determination of Chlorpromazine and Promethazine and Their Metabolites in Animal Food Using Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry

建立了动物源食品中吩噻嗪类药物氯丙嗪、异丙嗪及其代谢物氯丙嗪亚砜和异丙嗪亚砜同时测定的固相萃取超高效液相色谱-电喷雾电离串联四极杆质谱(uPlC-ESIMS/MS)方法。样品用乙酸乙酯和氢氧化钠溶液提取,OASIS Hlb柱富集净化,超高效液相色谱-电喷雾串联四极杆质谱仪检测,采用多反应监测正离子模式,可以一次性对动物源性食品中的吩噻嗪类药物氯丙嗪、异丙嗪及其代谢物氯丙嗪亚砜和异丙嗪亚砜进行定性和定量测定。方法在1--100μg/l范围具有良好的线性,回收率为76%--96%,定量下限(S/n>10)为异丙嗪5μg/kg,氯丙嗪、氯丙嗪亚砜、异丙嗪亚砜1μg/kg。检出限(S/n=3)为异丙嗪1.5μg/kg,氯丙嗪、氯丙嗪亚砜、异丙嗪亚砜0.3μg/kg。A method for the simultaneous detection residues of chlorpromazine and promethazine and their metabolites chlorpromazine sulphoxide and promethazine sulphoxide in animal foods was developed by using solid phase extraction(SPE) combination with ultra performance liquid chromatography-electrospray ionization triple-quadruple tandem mass spectrometry(UPLC-ESI MS/MS).The chlorpromazine and promethazine and their metabolites in the test sample was extracted with ethyl acetate and sodium hydroxide solution.The homogenate was centrifuged and the supernatant was collected.The extract was cleaned up and concentrated on an Oasis HLB cartridge,and then analyzed by UPLC-ESI MS/MS under the positive ion mode using multiple reaction monitoring(MRM) mode.Extraction and chromatographic conditions were optimized and the best separation was obtained.The cabalibration curves were linear in the range of 1-100 μg/L.The average recoveries were between 76% and 96%(n=6) in the spiked range of 5-100 μg/kg in pork,fish,dullfrog and milk.The limits of quantification(S/N>10) were in the range of 1-5 μg/kg and the limits of detection(S/N=3) were 0.3-1.5 μg/kg.国家质量监督检验检疫总局科研计划资助项目(2007IK137

儿童骶尾部肿瘤的多排螺旋CT诊断

【目的】探讨多排螺旋CT（MDCT）在儿童骶尾部肿瘤（SCT）的应用价值，以期提高对儿童SCT的诊断能力。 【方法】纳入经穿刺活检或手术病理证实的54例儿童SCT（男22例，女32例，年龄1 d至16岁），所有病例术前均行64排螺 旋CT平扫及单期增强扫描。回顾性分析患儿的CT特点及临床资料。【结果】儿童SCT好发于4岁以下儿童，女性多见，其中 生殖细胞肿瘤最常见，其次为神经源性肿瘤。54例儿童SCT中，恶性39例，良性15例。在CT检查中，以实性成分为主的肿 瘤37例（68%），31例为恶性肿瘤。以囊性成分为主的肿瘤8例（15%），均为良性肿瘤。囊实性或实性肿块伴有明显坏死的 肿瘤9例（17%），8例为恶性肿瘤。【结论】儿童恶性SCT发病率高于良性，大部分恶性SCT以实性成分为主、囊实性或实性 肿块中伴有明显坏死，而大部分的良性肿瘤以囊性成分为主；大多数SCT在结合CT表现及患儿临床资料的基础上于术前可 做出明确诊断

基于UPLC-oaTOF-MS的糖尿病及糖尿病肾病的代谢组学研究

Ultra performance liquid chromatography(UPLC) coupled with orthogonal acceleration time-of-flight(oaTOF) mass spectrometry is becoming an effective and high-throughput analytical technique in metabonomics research.In this paper,an UPLC-oaTOF-MS system was employed to investigate the serum profiles of 40 diabete mellitus(DM)/diabetic nephropathy(DN) patients and 25 healthy volunteers in an effort to find potential biomarkers.The UPLC system produced information-rich chromatograms with typical measured peak widths of 4 s,and generated peak capacities of 225 in 15 min.After the UPLC-oaTOF-MS analysis,principal component analysis(PCA) was used for group differentiation and marker selection.In the projection of positive mode,DM/DN patients and the controls were obviously divided into three groups.Furthermore,two potential markers were identified as phytosphingosine and sphinganine,respectively.Phytosphingosine and sphinganine with decreased concentrations in the patients,were closely related to the sphingolipid me..

圈养豚鹿繁殖成活率低的病因学调查研究

豚鹿是十分濒危的动物,在中国野外已宣布绝迹。为了弄清圈养豚鹿繁殖成活率低的原因,分别对成都动物园近年来圈养豚鹿的繁殖情况及死亡规律,豚鹿血清中的部分矿物质元素及维生素,以及豚鹿死亡个体的器官组织进行了切片分析。结果发现天气寒冷及母体的弃子行为是引起幼子死亡的一个重要因素,肺炎是导致豚鹿幼体死亡的直接原因,矿物质元素铜（Cu）、硒（Se）及维生素E测定值偏低与豚鹿繁殖成活率也有一定的关系。为切实提高豚鹿繁殖成活率应适当改善现有的环境条件,采取防寒保暖措施;加强对豚鹿产子期间的观察,及时发现弃子幼体施行人工辅助育幼;加强消毒工作,保持环境的清洁卫生;加强营养研究,适当调整饲料矿物质及维生素水平