Diverse Biological Activity of Odontioda Marie Noel ‘Velano’ Extracts

Background: Several pharmacologically active substances have been isolated from orchid plants other than Odontioda Marie Noel ‘Velano’. Whether or not MeOH extract fractions from O. Marie Noel ‘Velano’ bulbs exert various biological activities was investigated. Materials and Methods: The MeOH extract was stepwise separated by organic solvents into n-hexane, EtOAc, n-BuOH and H2O layer fractions. Cytotoxic activity against human tumor and normal cells was determined by the MTT method. Nitric oxide (NO) was determined by the Griess method. Osteoclastogenesis was monitored by tartrate-resistant acid phosphatase (TRAP) activity. Result: The EtOAc fraction showed the highest tumor-specific cytotoxicity, followed by the n-hexane and other fractions. The EtOAc and n-BuOH fractions protected the cells from the cytotoxicity induced by UV irradiation. The EtOAc and n-hexane fractions inhibited NO production by lipopolysaccharide (LPS)-stimulated mouse macrophage-like cells. The EtOAc fraction most strongly inhibited the receptor activator for nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis, followed by the n-BuOH, n-hexane and H2O fractions. Conclusion: Most of the biological activities tested were concentrated in the EtOAc fraction, and separation from cytotoxic substances is needed to identify the active principle(s)

Influence of siblings on multifaceted child development in Theory of Mind

Article信州大学教育学部研究論集 8: 29-43(2015)departmental bulletin pape

キョウトシリツ トウジキ シケンジョ ニオケル セイヨウ ユウヤク ノ ケンキュウ ト オウヨウ ジギョウホウコク ヲ テガカリ ニ

研究例会発表要旨第228回意匠学会研究例会（2016年11月19日　於：兵庫県立大学

Lipid Transfer Proteinに対するモノクローナル抗体を用いたリンゴ使用量の評価系の確立

In order to establish evaluation system for apple content in foods, monoclonal antibodies were raisedagainst apple lipid transfer protein (LTP). A sandwich-type of enzyme-linked immunosorbent assay (ELISA)was developed by using two of the five monoclonal antibodies obtained. This ELISA showed a practicalworking range of 0.03-10 ng/mL for apple LTP and no cross-reactivity with other fruits tested except plum.LTP could be determined with this ELISA in peel selectively and in foods with apple labeling such as applejuice, vegetable juice and even in curry roux